FastQCFastQC Report
Thu 26 May 2016
SRR938158_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938158_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1621052
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178991.1041595211011122No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147220.9081756785100046No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96910.597821661488959No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35320.217883201772676No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30520.18827280062576646No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27490.16958123490177981No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73800.048.4730341
GCGTATC459.6188346E-442.228831
GTATCAA127050.040.0849841
CGTATCA651.327203E-436.544182
TATCAAC159600.031.879992
ATCAACG160300.031.6469843
TCAACGC162000.031.4292624
CAACGCA164450.030.932145
AACGCAG168300.030.2245456
ACGCAGA191050.026.4762827
CGCAGAG190950.026.4155258
GCAGAGT197950.025.1694899
GAGTACT107100.025.0339212-13
GTACATG122750.023.7246911
GTACTTT112550.023.71620614-15
TACATGG121100.023.5379522
CCATATA5900.023.3511122
TGGTATC36100.023.161522
AGTACTT109000.022.68029212-13
ACATGGG122550.022.5194323
Produced by FastQC (version 0.11.5)