##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938158_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1621052 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.53143575900094 34.0 31.0 34.0 31.0 34.0 2 32.750516948253356 34.0 31.0 34.0 31.0 34.0 3 32.68975887263333 34.0 31.0 34.0 31.0 34.0 4 36.215095505881365 37.0 35.0 37.0 35.0 37.0 5 36.19581481655123 37.0 35.0 37.0 35.0 37.0 6 36.26461828491622 37.0 37.0 37.0 35.0 37.0 7 36.22981187525138 37.0 37.0 37.0 35.0 37.0 8 36.230311550770736 37.0 37.0 37.0 35.0 37.0 9 37.9274915301915 39.0 38.0 39.0 35.0 39.0 10-11 37.90332204025534 39.0 38.0 39.0 35.0 39.0 12-13 37.84686117410176 39.0 38.0 39.0 35.0 39.0 14-15 39.08539578002433 40.0 38.5 41.0 36.0 41.0 16-17 39.03646705966249 40.0 38.0 41.0 36.0 41.0 18-19 39.09345412731979 40.0 38.5 41.0 36.0 41.0 20-21 39.13125889854243 40.0 39.0 41.0 36.0 41.0 22-23 39.13690307281938 40.0 39.0 41.0 35.5 41.0 24-25 39.08198873324236 40.5 39.0 41.0 35.0 41.0 26-27 38.911785988358176 40.0 38.0 41.0 35.0 41.0 28-29 38.820804329534155 40.0 38.0 41.0 35.0 41.0 30-31 38.55755922697113 40.0 38.0 41.0 35.0 41.0 32-33 38.2999879090862 40.0 37.5 41.0 34.0 41.0 34-35 38.08023616762448 40.0 37.0 41.0 34.0 41.0 36-37 37.97530153258501 40.0 37.0 41.0 34.0 41.0 38-39 37.904218371773396 40.0 37.0 41.0 33.0 41.0 40-41 37.751761201984884 40.0 37.0 41.0 33.0 41.0 42-43 37.524550723850936 40.0 37.0 41.0 33.0 41.0 44-45 37.19605355041047 40.0 36.0 41.0 32.5 41.0 46-47 36.716285473877456 40.0 36.0 41.0 31.0 41.0 48-49 36.25642206419042 40.0 35.0 41.0 30.0 41.0 50-51 35.93905223274762 39.0 35.0 41.0 29.0 41.0 52-53 35.50066715935084 39.0 35.0 40.5 28.0 41.0 54-55 35.20339106950301 39.0 35.0 40.0 27.0 41.0 56-57 34.958797743687434 38.0 35.0 40.0 26.0 41.0 58-59 34.618007010262474 38.0 34.0 40.0 26.0 41.0 60-61 34.246716021447796 37.5 34.0 40.0 25.0 41.0 62-63 33.843503477988364 37.0 34.0 40.0 23.5 41.0 64-65 33.43381088330294 36.5 33.0 39.5 22.5 41.0 66-67 32.95484444669265 36.0 33.0 39.0 21.5 40.5 68-69 32.46034334493896 35.0 32.0 38.5 19.5 40.0 70-71 31.91785735436001 35.0 31.5 38.0 18.0 40.0 72-73 31.479247735421197 35.0 31.0 37.0 16.0 39.5 74-75 30.9554853885008 35.0 30.5 36.5 10.5 39.0 76-77 29.705817271746987 33.5 29.5 35.0 9.0 37.5 78-79 30.39836908378016 34.0 30.5 36.0 7.5 38.0 80-81 30.244185257474776 34.0 30.5 35.5 6.5 37.0 82-83 29.833402938338807 34.0 30.0 35.0 2.0 37.0 84-85 29.37208491769542 34.0 29.5 35.0 2.0 36.0 86-87 28.868308974665833 34.0 29.0 35.0 2.0 36.0 88-89 28.30483568694897 34.0 28.5 35.0 2.0 35.5 90-91 27.827868569299444 33.0 27.0 35.0 2.0 35.0 92-93 27.270244261134128 33.0 26.0 35.0 2.0 35.0 94-95 26.60609283354266 33.0 25.0 35.0 2.0 35.0 96-97 25.875414545616056 32.0 23.5 34.5 2.0 35.0 98-99 24.953933618415697 32.0 17.5 34.0 2.0 35.0 100-101 21.557083609902705 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1260955113814859 1101 2 0.051933284693205906 1101 3 0.19689442748595098 1101 4 -0.005658106947905139 1101 5 0.04485907280399459 1101 6 0.10018007609565416 1101 7 0.05109016667636723 1101 8 0.10062744621346553 1101 9 0.22989332528138817 1101 10-11 0.2819207055642394 1101 12-13 0.11951025971524132 1101 14-15 0.29741393176747266 1101 16-17 0.3561909871947506 1101 18-19 0.3403205368107507 1101 20-21 0.3626468525553861 1101 22-23 0.28475467098297 1101 24-25 0.3092525755604001 1101 26-27 0.2854796158502211 1101 28-29 0.17044290273253182 1101 30-31 0.18221556166306385 1101 32-33 0.23563124072259 1101 34-35 0.32692974218708315 1101 36-37 0.34882286745666846 1101 38-39 0.40747552719049907 1101 40-41 0.4468274202078959 1101 42-43 0.47651237659917456 1101 44-45 0.31423768750735803 1101 46-47 0.253015078019736 1101 48-49 0.35917768927587446 1101 50-51 0.4229379186720408 1101 52-53 0.3316096891365987 1101 54-55 0.4316231379145492 1101 56-57 0.4958048296098667 1101 58-59 0.5805123249676711 1101 60-61 0.5112528931365858 1101 62-63 0.6708261391262198 1101 64-65 0.6728286163105679 1101 66-67 0.6619215916807661 1101 68-69 0.7914338777562406 1101 70-71 0.7289084665280257 1101 72-73 0.6291459187777271 1101 74-75 0.6018834106642714 1101 76-77 0.7309378752856581 1101 78-79 1.0425006266797752 1101 80-81 1.2738101033130533 1101 82-83 1.1838770466231239 1101 84-85 1.288329177669162 1101 86-87 1.4577034018321307 1101 88-89 1.7772142251024654 1101 90-91 1.8513070366512743 1101 92-93 2.002612154008535 1101 94-95 2.1137897099821252 1101 96-97 2.234939581343422 1101 98-99 2.507097172770788 1101 100-101 2.9781820119467284 1102 1 0.10620078572366509 1102 2 0.13253975603088008 1102 3 0.23883569477921185 1102 4 0.0922097956728507 1102 5 -0.0037161627423216714 1102 6 0.04954922452979815 1102 7 0.10109165035731138 1102 8 0.014107309791398848 1102 9 0.1288455593698714 1102 10-11 0.20146475605151437 1102 12-13 0.14399075601130562 1102 14-15 0.1952644131022936 1102 16-17 0.3056840947788473 1102 18-19 0.19841530860626477 1102 20-21 0.23929242223621827 1102 22-23 0.17229476626400242 1102 24-25 0.1265298487271238 1102 26-27 0.3024060224036873 1102 28-29 0.1469208164665332 1102 30-31 0.141093669137085 1102 32-33 0.18369738226191856 1102 34-35 0.1730275177186016 1102 36-37 0.2965602061542256 1102 38-39 0.2895994691106054 1102 40-41 0.0479733005045162 1102 42-43 0.1336765454171811 1102 44-45 -0.007676567220563868 1102 46-47 0.017776506909775946 1102 48-49 0.1250860992580769 1102 50-51 0.2678700474478859 1102 52-53 0.2808874236802339 1102 54-55 0.16434016932742423 1102 56-57 0.30616360280673405 1102 58-59 0.3871037450800685 1102 60-61 0.5788817029660223 1102 62-63 0.3425531150302845 1102 64-65 0.3267550104202641 1102 66-67 0.43626616365795456 1102 68-69 0.5756221941995321 1102 70-71 0.8494826701981708 1102 72-73 0.8204838065329518 1102 74-75 0.6868839706341738 1102 76-77 0.729582939557762 1102 78-79 0.8898578558912504 1102 80-81 0.8370936423994308 1102 82-83 0.8908261295647151 1102 84-85 1.0249387178333578 1102 86-87 0.7718637484965782 1102 88-89 1.0221299855272399 1102 90-91 1.1833658401918186 1102 92-93 1.245905437528478 1102 94-95 1.4335746432499832 1102 96-97 1.659715521590325 1102 98-99 1.8996671857601726 1102 100-101 2.434869536295846 1103 1 0.2627854451471663 1103 2 0.30638699089461596 1103 3 0.4169970226852442 1103 4 0.24317203579808933 1103 5 0.14398315681523854 1103 6 0.24183145306055565 1103 7 0.2583772794084709 1103 8 0.26220262361487556 1103 9 0.2681123778474941 1103 10-11 0.3064776757732872 1103 12-13 0.31305893447151334 1103 14-15 0.4982648789115487 1103 16-17 0.4380982725239946 1103 18-19 0.5300469719062448 1103 20-21 0.47294003583785127 1103 22-23 0.5288401758998305 1103 24-25 0.4406539073807423 1103 26-27 0.496158144204351 1103 28-29 0.4395451835919033 1103 30-31 0.39983520590161703 1103 32-33 0.4270999215427267 1103 34-35 0.44080383678779356 1103 36-37 0.4977971383998465 1103 38-39 0.4055527032141626 1103 40-41 0.5884643063254416 1103 42-43 0.6247810547440835 1103 44-45 0.6657739402633993 1103 46-47 0.6736308721516124 1103 48-49 0.7629952472782762 1103 50-51 0.7655751191295082 1103 52-53 0.7741763303142406 1103 54-55 0.5687745494345506 1103 56-57 0.6654502943090392 1103 58-59 0.711569220493125 1103 60-61 0.8824833221325434 1103 62-63 0.8000694493707954 1103 64-65 0.7513294378301509 1103 66-67 0.802439895850334 1103 68-69 0.7555054937532404 1103 70-71 0.8660916464441542 1103 72-73 0.8668025035302271 1103 74-75 0.6795398616551118 1103 76-77 0.4394015333551664 1103 78-79 0.9035462193442854 1103 80-81 1.0162525690756397 1103 82-83 1.0412458654407146 1103 84-85 1.1173042957695678 1103 86-87 1.115328151307672 1103 88-89 1.2546748267178636 1103 90-91 1.1778906075973516 1103 92-93 1.52545697486822 1103 94-95 1.5199441957911262 1103 96-97 1.6617279191094667 1103 98-99 1.9237519495114164 1103 100-101 2.2750498710243505 1104 1 0.14106985745922174 1104 2 0.13763624634273697 1104 3 0.19429717494923437 1104 4 0.1312830313402884 1104 5 -0.00933446626481782 1104 6 0.06336824818890818 1104 7 0.04176699012690932 1104 8 0.04872020304792812 1104 9 0.04822455866623443 1104 10-11 0.11400688473133158 1104 12-13 0.06831859913035032 1104 14-15 0.13457418403316979 1104 16-17 0.17726560574186578 1104 18-19 0.061079143555112125 1104 20-21 0.07245145964128596 1104 22-23 0.1193586643118465 1104 24-25 0.08952624288608746 1104 26-27 0.03371434222962222 1104 28-29 0.05560092562607366 1104 30-31 0.18451205286579153 1104 32-33 0.22021785956196993 1104 34-35 0.14826051763306225 1104 36-37 0.23192929873652446 1104 38-39 0.2950787227371876 1104 40-41 0.36447106560517994 1104 42-43 0.3941481631198016 1104 44-45 0.34266770484786235 1104 46-47 0.48292247921241227 1104 48-49 0.5651794496642495 1104 50-51 0.7006644603499979 1104 52-53 0.7463901546029987 1104 54-55 0.8680446132933284 1104 56-57 0.9130334413584151 1104 58-59 0.891402901367421 1104 60-61 0.8886779937939266 1104 62-63 0.9500171930907868 1104 64-65 1.041403347430787 1104 66-67 0.8119063412119942 1104 68-69 0.9223799675615325 1104 70-71 0.859644703086154 1104 72-73 0.9701272843191369 1104 74-75 0.8723460138810566 1104 76-77 0.7563806698852105 1104 78-79 1.0466558484147974 1104 80-81 1.2634608123536495 1104 82-83 1.1435775766937297 1104 84-85 1.2927507516024974 1104 86-87 1.3360964187316888 1104 88-89 1.4359450547534571 1104 90-91 1.4638746123704927 1104 92-93 1.5228296452342036 1104 94-95 1.7912010894656127 1104 96-97 1.8744592807859917 1104 98-99 1.8851150876111475 1104 100-101 2.072761256186169 1105 1 0.06568423047635719 1105 2 0.05561165860230233 1105 3 0.1305949001205633 1105 4 -0.007005140709196667 1105 5 0.009281632603752143 1105 6 0.004537328213800151 1105 7 -0.01569355566044095 1105 8 -0.03135170492070216 1105 9 -0.03923111952792624 1105 10-11 0.04140187976217646 1105 12-13 0.10249181171928967 1105 14-15 -0.005702591679117575 1105 16-17 0.02455596632744772 1105 18-19 0.041690775071536734 1105 20-21 0.011154392761156373 1105 22-23 0.00443719657074837 1105 24-25 -0.0014108553211826802 1105 26-27 0.0280005627278328 1105 28-29 0.05430522046830788 1105 30-31 -0.02073543076908635 1105 32-33 -0.07717797808160043 1105 34-35 -0.016675282047799556 1105 36-37 0.048134020527619725 1105 38-39 0.0813103178557526 1105 40-41 0.10666956259472471 1105 42-43 0.06919550366098548 1105 44-45 0.1400443580051416 1105 46-47 0.00846436571699627 1105 48-49 0.17322252673547212 1105 50-51 0.28304452533650704 1105 52-53 0.25179039941209425 1105 54-55 0.321878839771081 1105 56-57 0.28063377810256895 1105 58-59 0.24890411074239438 1105 60-61 0.3481688224809787 1105 62-63 0.407407320528435 1105 64-65 0.5249945929854363 1105 66-67 0.5795854095832453 1105 68-69 0.5646237173687396 1105 70-71 0.7038907490130626 1105 72-73 0.631829933809545 1105 74-75 0.44952576649898646 1105 76-77 0.4029678250064528 1105 78-79 0.7272595428202244 1105 80-81 0.8043529210597171 1105 82-83 0.8907790867761207 1105 84-85 1.1003057151557343 1105 86-87 1.0915322112653882 1105 88-89 1.1341153437771467 1105 90-91 0.9931828649346279 1105 92-93 1.1947508549251538 1105 94-95 1.4033462611065772 1105 96-97 1.232493894113265 1105 98-99 1.7365109282640532 1105 100-101 1.8281184299426947 1106 1 0.035113399829846514 1106 2 -6.549101445685324E-4 1106 3 0.10260416667736649 1106 4 0.050057026423189654 1106 5 0.07368927751311105 1106 6 -0.036690490562925504 1106 7 0.020572595136059135 1106 8 -0.06504298289353017 1106 9 -0.10972591306043711 1106 10-11 -0.04647442484004927 1106 12-13 -0.06745875072249419 1106 14-15 -0.10409930420091484 1106 16-17 -0.05494453819834888 1106 18-19 0.010424728762636448 1106 20-21 -0.0034742434070764716 1106 22-23 -0.03413701051768925 1106 24-25 4.079751342587201E-4 1106 26-27 -0.06441722046339748 1106 28-29 -0.13352839348884515 1106 30-31 0.043641614202378776 1106 32-33 0.01650111962324985 1106 34-35 -0.09762928362406598 1106 36-37 -0.14367795852709264 1106 38-39 -0.10677817119395172 1106 40-41 -0.03342777506140493 1106 42-43 -0.18164412878152092 1106 44-45 -0.111488251079912 1106 46-47 -0.4114264895288784 1106 48-49 -0.15374937804349287 1106 50-51 0.06474120979591191 1106 52-53 -0.002803322973328193 1106 54-55 0.10240750221047534 1106 56-57 0.2242892168310462 1106 58-59 0.05330669546690814 1106 60-61 0.15216614461324696 1106 62-63 0.08652480339247148 1106 64-65 -0.004226481863497611 1106 66-67 0.23491032121659572 1106 68-69 -0.19529812591180473 1106 70-71 -0.12155265155057293 1106 72-73 -0.034729259904480614 1106 74-75 -0.015761962478109126 1106 76-77 0.06667077573607472 1106 78-79 0.33102729078420623 1106 80-81 0.2912177111292209 1106 82-83 0.48379132973663985 1106 84-85 0.35097667476978955 1106 86-87 0.4892302749610238 1106 88-89 0.6598006331182198 1106 90-91 0.43667709423414536 1106 92-93 0.20586273725421478 1106 94-95 0.660988782183221 1106 96-97 0.6373963773355449 1106 98-99 0.8941569571384527 1106 100-101 1.4062105909849443 1107 1 0.06665571859221586 1107 2 0.09898081570540995 1107 3 0.12494406593982177 1107 4 0.09065560530427774 1107 5 0.02100444211106378 1107 6 0.03624304064772588 1107 7 0.05695503802147073 1107 8 0.030252933638223567 1107 9 -0.006728521443243096 1107 10-11 0.02834231378199803 1107 12-13 -0.005211718882236482 1107 14-15 0.13257208453806157 1107 16-17 0.06085023154440705 1107 18-19 -0.008786797591028517 1107 20-21 0.048662494315536264 1107 22-23 0.021192747532317924 1107 24-25 0.09298307857013555 1107 26-27 0.20966534834393968 1107 28-29 0.09539092653273684 1107 30-31 0.17235743539323067 1107 32-33 0.19500107465113814 1107 34-35 0.376669151346249 1107 36-37 0.35706689216547716 1107 38-39 0.21181273994115202 1107 40-41 0.36820415064465095 1107 42-43 0.3392510412130818 1107 44-45 0.3635606560976399 1107 46-47 0.395030382210642 1107 48-49 0.4744295884972516 1107 50-51 0.4833107591752963 1107 52-53 0.22252947069458884 1107 54-55 0.3638557168174188 1107 56-57 0.43489645284472545 1107 58-59 0.4426499901242167 1107 60-61 0.41728520829376237 1107 62-63 0.6968217317968595 1107 64-65 0.6889428156720285 1107 66-67 0.8516607484517138 1107 68-69 0.7110474398374294 1107 70-71 0.7850037209984464 1107 72-73 0.629829338764349 1107 74-75 0.8163065300565187 1107 76-77 0.7932403026293997 1107 78-79 0.9206314237217832 1107 80-81 0.8902210165798365 1107 82-83 0.9966168154355088 1107 84-85 1.1972844639405054 1107 86-87 1.2622082161374948 1107 88-89 1.2544894260665913 1107 90-91 1.337516386373018 1107 92-93 1.4454545028595405 1107 94-95 1.265891867270465 1107 96-97 1.2854361551732865 1107 98-99 1.6220391077051026 1107 100-101 2.233649766196315 1108 1 -0.0018400863839147519 1108 2 -0.02789200980370765 1108 3 0.11107502490017396 1108 4 0.030592614152254782 1108 5 -0.029928977017611658 1108 6 -0.012349428837026721 1108 7 -0.0019713622059143177 1108 8 -0.060244376704062574 1108 9 -0.04520944382214509 1108 10-11 -3.751116207908467E-4 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sequence quality scores pass #Quality Count 2 9.0 3 4.0 4 12.0 5 38.0 6 67.0 7 179.0 8 402.0 9 777.0 10 1214.0 11 2227.0 12 3433.0 13 5256.0 14 7114.0 15 9619.0 16 13712.0 17 20720.0 18 20885.0 19 12774.0 20 9942.0 21 9953.0 22 10187.0 23 10883.0 24 12310.0 25 14478.0 26 16631.0 27 19811.0 28 24409.0 29 29498.0 30 36138.0 31 44677.0 32 55979.0 33 74035.0 34 104944.0 35 164132.0 36 243462.0 37 357489.0 38 263128.0 39 20522.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.23633541675406 19.594559582295943 15.251824124087321 20.91728087686268 2 15.651877777428172 21.109533777660147 40.01982874724146 23.218759697670215 3 19.126485398033 26.34751558657722 30.548580890418307 23.977418124971468 4 12.546112030952738 17.942731016648448 36.92614425693932 32.58501269545949 5 13.035040338152031 38.353692615755506 33.23132101124829 15.379946034844169 6 29.867493291385212 37.059375096388145 17.573794762653836 15.49933684957281 7 27.274942444782766 35.09393899763857 20.048092226529437 17.58302633104922 8 26.85716048240338 34.16168532741125 19.408654884180006 19.572499306005366 9 27.475306159210195 16.340499872922027 20.27831309544666 35.90588087242112 10-11 25.025847412667822 27.66583058408984 25.791862321504798 21.516459681737537 12-13 26.330278115692774 25.389438463417584 27.234289831541492 21.045993589348154 14-15 23.201414883668136 25.92202470987976 26.88803320312982 23.98852720332229 16-17 21.016920504043366 29.501817031049608 27.446668858660217 22.034593606246812 18-19 20.983164019414552 26.655776619133746 31.608301275961537 20.752758085490164 20-21 22.32781551733072 25.686622020761828 31.674091886009826 20.311470575897626 22-23 21.845320199475402 25.786526280464784 31.627733101714195 20.740420418345618 24-25 21.255255162628583 26.17408738298299 31.848398389907622 20.72225906448081 26-27 20.90266969212235 26.556209768355192 31.73887088389685 20.802249655625605 28-29 20.80808859703371 26.490648793449918 32.045328714920444 20.655933894595933 30-31 20.99146262961125 26.28556376594299 31.841563135487227 20.881410468958535 32-33 20.819431082415353 26.420636024702183 31.54210342380168 21.217829469080787 34-35 21.282890302263933 26.42888278553825 31.45505682275662 20.8331700894412 36-37 21.354224343513454 26.194097736440497 31.349594258324203 21.102083661721842 38-39 20.963919427706198 26.36715110575268 31.479398484364225 21.1895309821769 40-41 21.04485048552675 26.342375417460453 31.54901159248147 21.063762504531326 42-43 21.173747432115135 26.306555552847605 31.159553302950105 21.360143712087158 44-45 20.99934696310079 26.577799768460324 31.049760671384863 21.373092597054022 46-47 21.24287637244342 26.978977356148683 30.34239679607949 21.435749475328407 48-49 21.2773045066875 26.861382446462823 30.217239295726557 21.644073751123116 50-51 21.45936712702615 27.189781697317546 29.86338501170845 21.487466163947854 52-53 21.610657770386144 27.270470040442873 29.535018000656365 21.58385418851462 54-55 21.182880006316886 27.354612930368678 29.63479888368788 21.827708179626566 56-57 21.236672235067104 27.59421042631575 29.53671442988874 21.632402908728405 58-59 21.285005046105862 27.104096598998673 29.657315126226674 21.95358322866879 60-61 21.26594335036754 27.28373303262326 29.785873617872838 21.664449999136362 62-63 21.087910813471748 27.443505822145127 29.665735584052825 21.802847780330303 64-65 20.954725696646374 27.29502199806052 29.71542553847748 22.034826766815623 66-67 20.991863604411833 27.4453062660949 29.53151646510486 22.03131366438841 68-69 21.0446072127964 27.382692508772866 29.423623399532218 22.149076878898516 70-71 20.99463804985898 27.661944218939304 29.26581010356238 22.07760762763934 72-73 21.196698193518777 27.603001014156238 28.979576225808916 22.220724566516065 74-75 21.110149458499787 27.94009692471309 28.66478064861584 22.284972968171285 76-77 21.15400986519865 27.730356583255812 28.959620049202616 22.156013502342923 78-79 21.149444928355166 27.923225164892923 28.831616752577954 22.095713154173957 80-81 20.88406951301287 28.262285717458262 28.854278926793697 21.999365842735166 82-83 21.16658549442225 28.14516743433299 28.534208163470513 22.154038907774247 84-85 21.074434463532313 27.848934874702575 28.603908862708614 22.4727217990565 86-87 21.210534124308737 27.773958205136033 28.62458679986539 22.390920870689833 88-89 21.11651638350675 27.8278413202299 28.556843677342663 22.498798618920688 90-91 20.972074721165168 28.10436730965989 28.618591209735555 22.30496675943939 92-93 21.06157971718104 27.707109708168286 28.590032183102544 22.641278391548127 94-95 20.857363525268482 27.702398012153846 28.6419119447815 22.79832651779617 96-97 21.04643512693806 27.670551091532538 28.484377221751505 22.798636559777897 98-99 20.883132665760872 27.69459253970041 28.337479315072507 23.08479547946621 100-101 21.01247464155802 27.61034257377429 28.470516965210237 22.906665819457455 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3167.0 1 2042.0 2 1405.0 3 1857.5 4 1417.0 5 1295.5 6 2680.5 7 5880.5 8 9278.0 9 10249.5 10 7774.5 11 5018.5 12 4511.0 13 4663.0 14 4693.0 15 4604.5 16 4537.5 17 4598.5 18 4742.5 19 4854.0 20 5033.5 21 5433.5 22 6087.5 23 7055.5 24 8194.0 25 9566.5 26 11642.5 27 14072.0 28 15666.5 29 17785.5 30 20831.0 31 22839.5 32 25310.5 33 27957.0 34 30499.0 35 32312.5 36 34474.0 37 37631.0 38 39411.0 39 40463.0 40 42292.0 41 45810.0 42 48955.0 43 51722.5 44 53929.5 45 55393.0 46 55311.5 47 55439.5 48 59884.5 49 60808.0 50 57930.0 51 55029.0 52 52725.0 53 52459.0 54 50991.5 55 47050.0 56 41918.0 57 38693.5 58 36716.5 59 33521.5 60 29067.5 61 23711.5 62 18022.0 63 12943.5 64 9437.0 65 7351.0 66 5633.0 67 4701.0 68 3662.0 69 2381.0 70 1693.0 71 1342.5 72 1174.5 73 1011.0 74 810.0 75 660.5 76 437.5 77 234.0 78 123.0 79 57.0 80 22.0 81 19.0 82 14.5 83 5.0 84 1.5 85 1.5 86 2.0 87 1.0 88 1.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01067208208003198 3 0.00653896358660919 4 0.0 5 3.701300143363692E-4 6 1.233766714454564E-4 7 0.0 8 1.233766714454564E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 6.16883357227282E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0016655850645136614 26-27 0.02217695669232079 28-29 2.7759751075227693E-4 30-31 1.5422083930682052E-4 32-33 0.0037938326469477844 34-35 0.016439941470107064 36-37 0.008112016147538759 38-39 0.02134416416006396 40-41 0.024027606764002635 42-43 0.019215916577629836 44-45 0.010086042890666062 46-47 0.0023441567574636717 48-49 3.3928584647500515E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.5422083930682052E-4 68-69 1.5422083930682052E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.850650071681846E-4 82-83 8.327925322568307E-4 84-85 0.004256495164868246 86-87 9.561692037022871E-4 88-89 6.16883357227282E-5 90-91 0.004040585989838697 92-93 9.25325035840923E-4 94-95 6.16883357227282E-5 96-97 3.3928584647500515E-4 98-99 2.7759751075227693E-4 100-101 0.0026834426039386767 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1621052.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.052058915243414 #Duplication Level Percentage of deduplicated Percentage of total 1 72.86168232468721 32.82568804755558 2 14.011534667207638 12.624969706400279 3 5.043923875433431 6.817174668000894 4 2.4602968915680044 4.433657620316478 5 1.3736495883119058 3.094287109076392 6 0.8386867123053707 2.267073790452802 7 0.5734002930932565 1.8083004650518664 8 0.39322982004119605 1.4172650415781223 9 0.29073290776840727 1.1788304480444083 >10 1.8910939654809513 16.120297730298848 >50 0.16966435536281216 5.309525732552824 >100 0.08578109515643437 6.944309929822989 >500 0.004261491545372237 1.2113884572206806 >1k 0.0016496096304666724 1.3267676338976808 >5k 1.3746746920555603E-4 0.6001822872661357 >10k+ 2.7493493841111206E-4 2.0202813324639988 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17899 1.1041595211011122 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14722 0.9081756785100046 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9691 0.597821661488959 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3532 0.217883201772676 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3052 0.18827280062576646 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2749 0.16958123490177981 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.16883357227282E-5 0.0 10-11 0.0 0.0 0.0 6.16883357227282E-5 0.0 12-13 0.0 0.0 0.0 9.25325035840923E-5 0.0 14-15 0.0 0.0 0.0 1.233766714454564E-4 0.0 16-17 0.0 0.0 0.0 1.233766714454564E-4 0.0 18-19 0.0 0.0 0.0 1.233766714454564E-4 0.0 20-21 0.0 0.0 0.0 2.159091750295487E-4 0.0 22-23 0.0 0.0 0.0 5.860391893659179E-4 0.0 24-25 0.0 0.0 0.0 0.0013879875537613847 0.0 26-27 0.0 0.0 0.0 0.0038246768148091485 0.0 28-29 0.0 0.0 0.0 0.009561692037022871 0.0 30-31 0.0 0.0 0.0 0.025076308471289013 0.0 32-33 0.0 0.0 0.0 0.05203411118212124 0.0 34-35 0.0 0.0 0.0 0.09071269768027182 0.0 36-37 0.0 0.0 0.0 0.15542376185341372 0.0 38-39 0.0 0.0 0.0 0.2567468532779948 0.0 40-41 0.0 0.0 0.0 0.36063001063506905 0.0 42-43 0.0 0.0 0.0 0.4648524538386184 0.0 44-45 0.0 0.0 0.0 0.5865018518838383 3.08441678613641E-5 46-47 0.0 0.0 0.0 0.7248687889099177 6.16883357227282E-5 48-49 0.0 0.0 0.0 0.8748948213875927 6.16883357227282E-5 50-51 0.0 0.0 0.0 1.0325393633270248 6.16883357227282E-5 52-53 0.0 0.0 0.0 1.1827813049797293 6.16883357227282E-5 54-55 0.0 0.0 0.0 1.345669355455593 6.16883357227282E-5 56-57 0.0 0.0 0.0 1.5298090375879367 6.16883357227282E-5 58-59 0.0 0.0 0.0 1.7219682033642352 6.16883357227282E-5 60-61 0.0 0.0 0.0 1.9341452340825587 6.16883357227282E-5 62-63 3.08441678613641E-5 0.0 0.0 2.1482346032082873 6.16883357227282E-5 64-65 6.16883357227282E-5 0.0 0.0 2.3576356588190874 6.16883357227282E-5 66-67 6.16883357227282E-5 0.0 0.0 2.592143867069039 6.16883357227282E-5 68-69 6.16883357227282E-5 0.0 0.0 2.845713771057313 6.16883357227282E-5 70-71 6.16883357227282E-5 0.0 0.0 3.116957383230149 6.16883357227282E-5 72-73 6.16883357227282E-5 0.0 0.0 3.3917172305391805 6.16883357227282E-5 74-75 6.16883357227282E-5 0.0 0.0 3.6590127892257622 6.16883357227282E-5 76-77 6.16883357227282E-5 0.0 0.0 3.9485778371082483 6.16883357227282E-5 78-79 6.16883357227282E-5 0.0 0.0 4.265748415226655 6.16883357227282E-5 80-81 6.16883357227282E-5 0.0 0.0 4.600839454872515 9.25325035840923E-5 82-83 6.16883357227282E-5 0.0 0.0 4.9520928384777285 1.233766714454564E-4 84-85 6.16883357227282E-5 0.0 0.0 5.295110829263959 1.233766714454564E-4 86-87 6.16883357227282E-5 0.0 0.0 5.6434340169223445 1.233766714454564E-4 88-89 6.16883357227282E-5 0.0 0.0 6.017296175569939 1.233766714454564E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7380 0.0 48.473034 1 GCGTATC 45 9.6188346E-4 42.22883 1 GTATCAA 12705 0.0 40.084984 1 CGTATCA 65 1.327203E-4 36.54418 2 TATCAAC 15960 0.0 31.87999 2 ATCAACG 16030 0.0 31.646984 3 TCAACGC 16200 0.0 31.429262 4 CAACGCA 16445 0.0 30.93214 5 AACGCAG 16830 0.0 30.224545 6 ACGCAGA 19105 0.0 26.476282 7 CGCAGAG 19095 0.0 26.415525 8 GCAGAGT 19795 0.0 25.169489 9 GAGTACT 10710 0.0 25.03392 12-13 GTACATG 12275 0.0 23.724691 1 GTACTTT 11255 0.0 23.716206 14-15 TACATGG 12110 0.0 23.537952 2 CCATATA 590 0.0 23.351112 2 TGGTATC 3610 0.0 23.16152 2 AGTACTT 10900 0.0 22.680292 12-13 ACATGGG 12255 0.0 22.519432 3 >>END_MODULE