FastQCFastQC Report
Thu 26 May 2016
SRR938157_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938157_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1631138
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189001.1587002448597237No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150120.9203390516314377No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94220.5776335294745141No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34150.2093630336611617No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29970.18373675311347046No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28980.17766737087849097No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16660.10213728084319046No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66800.053.3977621
GTATCAA120450.043.132141
TATCAAC156450.033.076532
ATCAACG157900.032.7126053
TCAACGC159750.032.393264
CAACGCA162950.031.6696455
AACGCAG166600.031.0898916
ACGCAGA189750.027.2091967
CGCAGAG189850.027.069668
TGGTATC29250.025.6684072
GCAGAGT198450.025.3448039
GAGTACT108700.024.8400912-13
GTGGTAT31350.024.2180141
GTACATG122700.023.583431
GTACTTT114500.023.38134614-15
TACATGG120600.023.2078912
AGTACTT110050.022.87232212-13
GTAAGGT5600.022.0624164
CAGAGTA193350.021.85807610-11
ACATGGG124050.021.5281753
Produced by FastQC (version 0.11.5)