##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938157_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1631138 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.951780290815368 33.0 31.0 34.0 30.0 34.0 2 32.121288327535744 34.0 31.0 34.0 30.0 34.0 3 32.16642307395205 34.0 31.0 34.0 30.0 34.0 4 35.704278853168766 37.0 35.0 37.0 35.0 37.0 5 35.739035569032175 37.0 35.0 37.0 35.0 37.0 6 35.78944822571726 37.0 36.0 37.0 35.0 37.0 7 35.7358365754461 37.0 36.0 37.0 35.0 37.0 8 35.729141862920244 37.0 36.0 37.0 35.0 37.0 9 37.383302945550895 39.0 37.0 39.0 35.0 39.0 10-11 37.31158246573864 39.0 37.0 39.0 34.0 39.0 12-13 37.215320224285136 39.0 37.0 39.0 34.0 39.0 14-15 38.42940327550458 40.0 38.0 41.0 34.0 41.0 16-17 38.36434808091038 40.0 38.0 41.0 34.0 41.0 18-19 38.37456793968383 40.0 38.0 41.0 34.0 41.0 20-21 38.3125495819483 40.0 38.0 41.0 34.0 41.0 22-23 38.164753380768516 40.0 38.0 41.0 33.5 41.0 24-25 38.10227889976201 40.0 38.0 41.0 33.5 41.0 26-27 38.10018067140855 40.0 38.0 41.0 33.5 41.0 28-29 37.99949329854371 40.0 38.0 41.0 33.0 41.0 30-31 37.7619609744853 40.0 37.5 41.0 33.0 41.0 32-33 37.48834218809199 40.0 36.5 41.0 33.0 41.0 34-35 37.26301422687719 40.0 36.0 41.0 32.0 41.0 36-37 37.03762128035764 40.0 36.0 41.0 31.5 41.0 38-39 36.80536686656801 39.5 35.0 41.0 31.0 41.0 40-41 36.55771645317564 39.0 35.0 41.0 30.5 41.0 42-43 36.240468311080974 39.0 35.0 41.0 30.0 41.0 44-45 35.9136841885849 39.0 35.0 41.0 29.5 41.0 46-47 35.60200332528578 39.0 35.0 41.0 27.5 41.0 48-49 35.25778842746598 39.0 35.0 41.0 25.5 41.0 50-51 34.581737106241164 38.5 34.0 40.5 24.0 40.5 52-53 34.650412472764415 38.5 34.5 40.0 24.0 41.0 54-55 34.78261312041042 39.0 35.0 40.5 24.0 41.0 56-57 34.59472129274163 38.5 35.0 40.0 23.0 41.0 58-59 34.353747812876655 38.0 34.5 40.0 23.0 41.0 60-61 34.00347426152784 38.0 34.0 40.0 22.0 41.0 62-63 33.611253002504995 37.0 34.0 40.0 20.5 41.0 64-65 33.20618580402149 36.5 33.0 40.0 18.5 41.0 66-67 32.794230163235724 36.0 33.0 39.0 16.0 41.0 68-69 32.33617357942737 35.5 32.5 39.0 11.5 40.5 70-71 31.88456034989069 35.0 32.0 38.5 8.0 40.0 72-73 31.422515139736795 35.0 31.0 37.5 6.5 40.0 74-75 30.926516640529496 35.0 31.0 37.0 2.0 39.0 76-77 30.414007582436312 35.0 30.5 36.5 2.0 39.0 78-79 29.867037920764524 34.5 30.0 36.0 2.0 38.5 80-81 29.3600777493995 34.0 29.0 35.5 2.0 37.0 82-83 28.859933371670575 34.0 29.0 35.0 2.0 37.0 84-85 28.389975894130355 34.0 28.5 35.0 2.0 36.0 86-87 27.89981748938471 34.0 27.0 35.0 2.0 36.0 88-89 27.37353124015258 33.5 26.0 35.0 2.0 35.5 90-91 26.797830103890657 33.0 25.0 35.0 2.0 35.0 92-93 26.220843975187876 33.0 24.5 35.0 2.0 35.0 94-95 25.671182021386294 32.5 21.5 35.0 2.0 35.0 96-97 24.87724766390091 32.0 17.0 34.5 2.0 35.0 98-99 23.849532657567906 31.5 2.0 34.0 2.0 35.0 100-101 20.993837124755846 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3857750480103448 1101 2 0.3113611920379853 1101 3 0.346073587223394 1101 4 0.11126811103424927 1101 5 0.1894610958316818 1101 6 0.2801498282770183 1101 7 0.22995019113119497 1101 8 0.24719197322864517 1101 9 0.3004403074637949 1101 10-11 0.3721949067816226 1101 12-13 0.28395447811124797 1101 14-15 0.3577094268947292 1101 16-17 0.4494499010780473 1101 18-19 0.5028898041265677 1101 20-21 0.4406989387487883 1101 22-23 0.5132854869832784 1101 24-25 0.5242678906474865 1101 26-27 0.5168432150048687 1101 28-29 0.4703218467040031 1101 30-31 0.4236094663663934 1101 32-33 0.3537299110848622 1101 34-35 0.4433875105137446 1101 36-37 0.5774265510155203 1101 38-39 0.5079515902017633 1101 40-41 0.5078686126555354 1101 42-43 0.4853174225047425 1101 44-45 0.575954915448385 1101 46-47 0.6345690885629338 1101 48-49 0.8336759550955506 1101 50-51 0.767526440729533 1101 52-53 0.8425422153304041 1101 54-55 0.8614086496934945 1101 56-57 0.7228732233806525 1101 58-59 0.6273539447563579 1101 60-61 0.7122700357971397 1101 62-63 0.8142874690269153 1101 64-65 0.6580115750565838 1101 66-67 0.5465335012577697 1101 68-69 0.60165941785462 1101 70-71 0.6442741537952799 1101 72-73 0.8553036014580222 1101 74-75 0.891593757565385 1101 76-77 0.9767740912610599 1101 78-79 1.0437516314779849 1101 80-81 1.1643263245211877 1101 82-83 1.048016739758669 1101 84-85 1.0490190891356725 1101 86-87 0.974369775510727 1101 88-89 1.0490273240488435 1101 90-91 0.9810463527848974 1101 92-93 1.2833967024571997 1101 94-95 1.2148607615216456 1101 96-97 1.2468460065789628 1101 98-99 1.4309087218363175 1101 100-101 1.111069817348703 1102 1 0.4444136316489278 1102 2 0.3078660760428704 1102 3 0.15619263484244073 1102 4 0.21744179845793354 1102 5 0.21481976494035138 1102 6 0.30814128126847606 1102 7 0.2232346844156865 1102 8 0.22677835906503674 1102 9 0.25909415361763877 1102 10-11 0.32878831337502845 1102 12-13 0.22537312890489858 1102 14-15 0.2844455380058406 1102 16-17 0.32095096945411683 1102 18-19 0.3125166356283984 1102 20-21 0.3274701536450024 1102 22-23 0.4062914393642316 1102 24-25 0.4133704547500443 1102 26-27 0.39157230535896304 1102 28-29 0.354998281380432 1102 30-31 0.37695577283770376 1102 32-33 0.3923135496685006 1102 34-35 0.3858821686334082 1102 36-37 0.5021403788543495 1102 38-39 0.5705126402628125 1102 40-41 0.6721123550242751 1102 42-43 0.7772702613325748 1102 44-45 0.586795116913585 1102 46-47 0.6006252546191035 1102 48-49 0.6174709795155735 1102 50-51 0.7061673137454036 1102 52-53 0.7196865987247847 1102 54-55 0.8163765984114448 1102 56-57 0.6693475823550088 1102 58-59 0.8518235723509875 1102 60-61 0.9437375266396302 1102 62-63 0.8054771820916287 1102 64-65 0.5564433516133533 1102 66-67 0.6080223596216285 1102 68-69 0.6590197658399717 1102 70-71 0.7225174382885626 1102 72-73 0.5168573255117401 1102 74-75 0.6676239773456025 1102 76-77 0.6301928946798618 1102 78-79 0.7036989758003287 1102 80-81 0.8702519960718611 1102 82-83 0.9546330401249676 1102 84-85 1.069093112334695 1102 86-87 1.0252473701383202 1102 88-89 0.8317005535970701 1102 90-91 1.2120979400864869 1102 92-93 1.3246779906134876 1102 94-95 1.0262618604227427 1102 96-97 0.9474794009623544 1102 98-99 1.1819923604199545 1102 100-101 1.1450556232095117 1103 1 0.2539457446136808 1103 2 0.2523729939953867 1103 3 0.13167375993496222 1103 4 0.14224465104575756 1103 5 0.12145231632678843 1103 6 0.14408189505882518 1103 7 0.12466809556412528 1103 8 0.157687799365668 1103 9 0.1626164732669011 1103 10-11 0.19194879279198318 1103 12-13 0.12824600085159688 1103 14-15 0.16333154836853225 1103 16-17 0.1078929782225515 1103 18-19 0.20273328426473114 1103 20-21 0.17007249948569125 1103 22-23 0.3440227495048731 1103 24-25 0.45883179392900075 1103 26-27 0.29659458230481306 1103 28-29 0.3486105628813192 1103 30-31 0.4532205055143592 1103 32-33 0.5173669289317218 1103 34-35 0.5695335237549628 1103 36-37 0.4425442107737254 1103 38-39 0.48776232710446976 1103 40-41 0.4577732614753458 1103 42-43 0.5497874138691614 1103 44-45 0.5957681710615077 1103 46-47 0.6149807178087556 1103 48-49 0.5845413120327905 1103 50-51 0.44177982299780894 1103 52-53 0.60001918712085 1103 54-55 0.5559142628195772 1103 56-57 0.6977328952286612 1103 58-59 0.6301910921369043 1103 60-61 0.6501640340700234 1103 62-63 0.6972987672422235 1103 64-65 0.4996620500133986 1103 66-67 0.6282566590010106 1103 68-69 0.6822023798580759 1103 70-71 0.8058638198862411 1103 72-73 0.7084088115904343 1103 74-75 0.6988152601560564 1103 76-77 0.7165445287970407 1103 78-79 0.6953006383864455 1103 80-81 0.9134393786374808 1103 82-83 0.8169394513303416 1103 84-85 0.9552564955835727 1103 86-87 0.7990171560518924 1103 88-89 0.7777963942848807 1103 90-91 0.9275633360894453 1103 92-93 0.8728954790835672 1103 94-95 1.0317972900190533 1103 96-97 0.9604434446906502 1103 98-99 1.028224496159865 1103 100-101 1.144840326847838 1104 1 0.49324691861512093 1104 2 0.42336892762167366 1104 3 0.3453879332149512 1104 4 0.3256053121594249 1104 5 0.3610240353534806 1104 6 0.3694606580941766 1104 7 0.25206692812134435 1104 8 0.3468315653524172 1104 9 0.30676942138784113 1104 10-11 0.4054227548828848 1104 12-13 0.3089061307132184 1104 14-15 0.45311642408179154 1104 16-17 0.5263794017812842 1104 18-19 0.6320169138312082 1104 20-21 0.4127497474689008 1104 22-23 0.42991259668792026 1104 24-25 0.546683502461832 1104 26-27 0.6387094245688658 1104 28-29 0.6850546174494241 1104 30-31 0.47234803879958775 1104 32-33 0.49268912346180116 1104 34-35 0.6509077285165645 1104 36-37 0.7201348843488589 1104 38-39 0.6194406900611185 1104 40-41 0.6974827125148906 1104 42-43 0.6785004393823755 1104 44-45 0.7927929956171553 1104 46-47 0.8879270351170803 1104 48-49 0.7066497595962531 1104 50-51 0.8288617079587866 1104 52-53 0.8293741421939771 1104 54-55 0.8870679323939186 1104 56-57 0.9560747001738932 1104 58-59 0.9612103090320261 1104 60-61 0.8395775252486146 1104 62-63 0.8392127503208613 1104 64-65 0.8422810898245032 1104 66-67 0.9811810047739442 1104 68-69 0.9690776667997625 1104 70-71 1.0050775996461994 1104 72-73 0.8915817308532361 1104 74-75 0.8081285676919663 1104 76-77 0.995242797308876 1104 78-79 0.9952416103809405 1104 80-81 1.1632934482905277 1104 82-83 1.2377583009401043 1104 84-85 1.0940305167024782 1104 86-87 1.0370631651028503 1104 88-89 1.0555190610389964 1104 90-91 1.185933835204029 1104 92-93 1.2111964563249913 1104 94-95 1.3864588627874674 1104 96-97 1.5038519840754425 1104 98-99 1.4428109721879352 1104 100-101 1.5558351127073529 1105 1 0.5423910852298306 1105 2 0.37877361677799826 1105 3 0.3901827826256934 1105 4 0.28538893752575945 1105 5 0.3357699354594885 1105 6 0.33845579434160555 1105 7 0.2894055824831341 1105 8 0.44681909419197297 1105 9 0.4335683862815287 1105 10-11 0.48377126146142047 1105 12-13 0.3662290402349484 1105 14-15 0.4962681981043602 1105 16-17 0.4864966306894374 1105 18-19 0.4881987121725402 1105 20-21 0.5338930411023739 1105 22-23 0.41552789256620315 1105 24-25 0.4861726525522485 1105 26-27 0.5131263978182261 1105 28-29 0.5316040032447802 1105 30-31 0.6536467542700635 1105 32-33 0.7493853571713487 1105 34-35 0.7402659314223357 1105 36-37 0.6825117316394937 1105 38-39 0.5022694610282556 1105 40-41 0.57489276427021 1105 42-43 0.8815356080548185 1105 44-45 0.9047777808014175 1105 46-47 1.1134141898218317 1105 48-49 0.8677670196822973 1105 50-51 0.8170047521690478 1105 52-53 0.7943005623473738 1105 54-55 0.8438491258839704 1105 56-57 0.937169200392141 1105 58-59 0.7502107455226437 1105 60-61 0.8617107172421257 1105 62-63 0.841830914566053 1105 64-65 0.9157650643833435 1105 66-67 1.0174814350289836 1105 68-69 0.9680028086592145 1105 70-71 1.08984407716693 1105 72-73 1.1482873179331143 1105 74-75 1.1898388314570063 1105 76-77 1.246844561978083 1105 78-79 1.3306409992940758 1105 80-81 1.2965735193761425 1105 82-83 1.1778710090525912 1105 84-85 1.3654411608379142 1105 86-87 1.4181163546021338 1105 88-89 1.4816508188490616 1105 90-91 1.3310942455052093 1105 92-93 1.610498850787799 1105 94-95 1.679054111275338 1105 96-97 1.5260366196057795 1105 98-99 1.4885715570853577 1105 100-101 1.4710195299923825 1106 1 0.021643440674850467 1106 2 0.1371163160940796 1106 3 -0.0016610406083259477 1106 4 0.09952997726941248 1106 5 0.008629110934165851 1106 6 0.06462933205263965 1106 7 0.20014975963172787 1106 8 0.0703783270582079 1106 9 0.1823577831919465 1106 10-11 -0.3784725689465347 1106 12-13 -0.051499270540318776 1106 14-15 -0.1163432969456153 1106 16-17 -0.00942617767060483 1106 18-19 0.08578826690973784 1106 20-21 0.08651541663444817 1106 22-23 0.0019485128732483759 1106 24-25 -0.0033817857279174746 1106 26-27 0.06652296149893999 1106 28-29 -0.07691385987637034 1106 30-31 -0.03334509134665353 1106 32-33 0.1758240319047104 1106 34-35 -0.07509617341289498 1106 36-37 -0.32974375642784537 1106 38-39 -0.2022088736601546 1106 40-41 0.09209528471594552 1106 42-43 0.059397115061372574 1106 44-45 -0.04772024636391592 1106 46-47 0.12850786957695703 1106 48-49 0.23578288605563813 1106 50-51 0.24209831235844348 1106 52-53 0.10715925955910421 1106 54-55 -0.3299546189149183 1106 56-57 -0.19346981869055213 1106 58-59 -0.07394392471505284 1106 60-61 -0.18899412277891514 1106 62-63 0.028328056944928903 1106 64-65 -0.01643219516996197 1106 66-67 -0.21841728083499845 1106 68-69 -0.6303086242165783 1106 70-71 -0.36604812236437567 1106 72-73 -0.049800228099694976 1106 74-75 -0.011500902628792886 1106 76-77 0.13448714411974194 1106 78-79 0.3092148191802515 1106 80-81 0.26642651330220346 1106 82-83 0.2503427915386034 1106 84-85 0.2240561711205693 1106 86-87 0.34179156623348916 1106 88-89 0.18127179546200267 1106 90-91 -0.02026162995511882 1106 92-93 0.15589198293184836 1106 94-95 0.3090355188031957 1106 96-97 0.6501159634290161 1106 98-99 0.7403814834328664 1106 100-101 0.16494319922572842 1107 1 0.3303105536873119 1107 2 0.3048088851534274 1107 3 0.29652889338602506 1107 4 0.2016029046287855 1107 5 0.30203205796333776 1107 6 0.2708894751891435 1107 7 0.1918603440484361 1107 8 0.2267503672694673 1107 9 0.3299146134295299 1107 10-11 0.2551836840999897 1107 12-13 0.15391555148526948 1107 14-15 0.26716851496981064 1107 16-17 0.34846812152826345 1107 18-19 0.3607278381883816 1107 20-21 0.4073498132647657 1107 22-23 0.3689159014349457 1107 24-25 0.35444853644390406 1107 26-27 0.3660091023186496 1107 28-29 0.393479292395412 1107 30-31 0.43412232158724606 1107 32-33 0.5007683700991095 1107 34-35 0.4017776545709708 1107 36-37 0.5614963728308382 1107 38-39 0.614234405060337 1107 40-41 0.5754867051278083 1107 42-43 0.5521028379408435 1107 44-45 0.5390818843399003 1107 46-47 0.6384519067029899 1107 48-49 0.5484576132303474 1107 50-51 0.6370908645006139 1107 52-53 0.6216481745087279 1107 54-55 0.599987848354651 1107 56-57 0.5031953832546421 1107 58-59 0.5740619991633977 1107 60-61 0.43879900311353737 1107 62-63 0.3444880088313411 1107 64-65 0.335249343318047 1107 66-67 0.34692584391052605 1107 68-69 0.5051855795006119 1107 70-71 0.3816974490633811 1107 72-73 0.5697425494136503 1107 74-75 0.5395817965494345 1107 76-77 0.5576245703579907 1107 78-79 0.6317973835945416 1107 80-81 0.7827893996015831 1107 82-83 0.5763847748049358 1107 84-85 0.4076697011327859 1107 86-87 0.5627549563205996 1107 88-89 0.6870874326660257 1107 90-91 1.0056886708265118 1107 92-93 0.8170907941419117 1107 94-95 0.7470041460461552 1107 96-97 0.8139946414808428 1107 98-99 0.9639557455286436 1107 100-101 0.8711954572384464 1108 1 0.2322660177786986 1108 2 0.1763150593328433 1108 3 0.044822114903013244 1108 4 0.11877449108921212 1108 5 0.15699397765343548 1108 6 0.23535202694211677 1108 7 0.10544096459016572 1108 8 0.16207958610654316 1108 9 0.24296091994906988 1108 10-11 0.24168842857195472 1108 12-13 0.1136082374359404 1108 14-15 0.22466933070313644 1108 16-17 0.18048027995515525 1108 18-19 0.22269619399699536 1108 20-21 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-0.6916642540047206 2316 54-55 -0.8186851108177677 2316 56-57 -0.734772673020295 2316 58-59 -0.8117651409034679 2316 60-61 -0.5329042410276159 2316 62-63 -0.765292991226481 2316 64-65 -0.5890799570658558 2316 66-67 -0.6741067751185028 2316 68-69 -0.7414273211295708 2316 70-71 -0.7066389250252172 2316 72-73 -0.7200166418428005 2316 74-75 -0.6256300063094891 2316 76-77 -0.47013069066516877 2316 78-79 -0.8086775225751595 2316 80-81 -0.7681391284443926 2316 82-83 -0.7102430146981114 2316 84-85 -0.7333832962214295 2316 86-87 -0.7665120498060141 2316 88-89 -0.8182738728342187 2316 90-91 -0.6290752976243397 2316 92-93 -0.9293552176011062 2316 94-95 -1.1750925684480826 2316 96-97 -1.2387038028167119 2316 98-99 -1.0754246675571117 2316 100-101 -0.9043827978547938 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4635.0 3 1054.0 4 1046.0 5 1113.0 6 1318.0 7 1675.0 8 2088.0 9 2640.0 10 3397.0 11 4425.0 12 5716.0 13 7633.0 14 9107.0 15 11757.0 16 16013.0 17 23564.0 18 20117.0 19 13040.0 20 12041.0 21 12137.0 22 12879.0 23 13484.0 24 15014.0 25 16873.0 26 19440.0 27 22525.0 28 26393.0 29 30759.0 30 37195.0 31 45915.0 32 57226.0 33 74946.0 34 104667.0 35 161355.0 36 231496.0 37 320199.0 38 255857.0 39 30397.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.98918675793741 19.14757148754475 14.882163387779496 20.981078366738345 2 15.48321088008087 20.87325996361366 40.40546359464191 23.238065561663564 3 18.837637855750344 26.550014538575073 30.336239821463845 24.27610778421074 4 12.353474196059762 17.68475272744897 37.250299539326 32.71147353716527 5 13.022013219221623 38.23204569049939 33.218141700434664 15.527799389844319 6 29.687780028232986 37.0511876265881 17.744000491008407 15.5170318541705 7 27.185306480042552 35.15926630563155 20.214878679806596 17.440548534519305 8 26.805894967220194 33.88416166136693 19.627338260118556 19.68260511129431 9 27.77463924797964 16.301544218000032 20.296391713054547 35.627424820965786 10-11 24.902467450035758 27.56791232025465 25.930236731753816 21.59938349795578 12-13 26.32356948563348 25.47439337549345 27.29758111239861 20.904456026474456 14-15 23.32217091202646 25.828041458201955 26.92381540107424 23.92597222869734 16-17 21.023624462452993 29.465796367071757 27.444036832322144 22.06654233815311 18-19 20.971649711417747 26.69685658978808 31.643196577930087 20.688297120864082 20-21 22.409813454539208 25.695434655095823 31.64934803576293 20.245403854602035 22-23 21.93898788389471 25.739614931223258 31.609387734694135 20.712009450187896 24-25 21.234665931936405 26.167708727724143 31.87353762189169 20.724087718447766 26-27 20.924692505876568 26.502547100734635 31.74248561571371 20.830274777675083 28-29 20.790387810342754 26.50017914418162 32.00125523628627 20.70817780918936 30-31 20.92129929434992 26.31547463656858 31.8810699082253 20.88215616085619 32-33 20.81297969851466 26.4695109605934 31.47852975281147 21.23897958808047 34-35 21.330484479061536 26.421671334656526 31.399691391163238 20.848152795118704 36-37 21.349091486903752 26.171539248244297 31.309391394080016 21.169977870771934 38-39 21.008748687451543 26.39406139292058 31.3831562006261 21.214033719001776 40-41 21.063728453239538 26.375623819867595 31.49544722283813 21.065200504054733 42-43 21.205104961071235 26.318806143715644 31.10780452081496 21.368284374398158 44-45 20.99020519842552 26.64154754553543 31.01824115460064 21.350006101438407 46-47 21.296937934485584 26.936235791064256 30.334268739605463 21.4325575348447 48-49 21.302780840617714 26.892088960407396 30.16969223466846 21.635437964306433 50-51 21.467632516959874 27.22410026445901 29.798811850829786 21.509455367751332 52-53 21.521345591553995 27.37666555466159 29.53826850997861 21.563720343805805 54-55 21.16947896966441 27.406156404765586 29.699385291643054 21.724979333926946 56-57 21.289787483251008 27.65051097844784 29.545807155844596 21.51389438245656 58-59 21.316614323948027 27.056305394085022 29.715068172264626 21.912012109702324 60-61 21.350648092273424 27.164651497088244 29.925452217299796 21.559248193338533 62-63 21.161188095497977 27.28053726503546 29.818405693478816 21.73986894598774 64-65 21.003328853776026 27.138544030430328 29.93819069423327 21.919936421560376 66-67 21.061220860804823 27.32019811663496 29.72470004276891 21.893880979791305 68-69 21.106454969613463 27.170670478243782 29.6893405223504 22.033534029792357 70-71 21.01747433712612 27.534202087254233 29.516882004907323 21.931441570712323 72-73 21.23297374972171 27.443135436618405 29.253687679278155 22.070203134381725 74-75 21.11891671445086 27.792155624173997 29.063378705319227 22.025548956055918 76-77 21.309853411456654 27.54491017964072 29.134204708861844 22.01103170004078 78-79 21.321986787980723 27.661481172397323 29.084653620612638 21.931878419009315 80-81 21.13325682876825 28.07547891745325 29.02278243812466 21.76848181565384 82-83 21.41027150464445 27.986299673036687 28.640379601217592 21.963049221101276 84-85 21.37383984459314 27.635889027333555 28.76842022643879 22.221850901634518 86-87 21.490871890708554 27.553183564344963 28.763316009377192 22.19262853556929 88-89 21.461148565764812 27.441002787018775 28.869625911423835 22.22822273579258 90-91 21.41331504553649 27.7535821610557 28.81330927948248 22.019793513925325 92-93 21.550210219252964 27.261593336520377 28.880148194530776 22.308048249695887 94-95 21.36849417794197 27.289066369774677 28.924978032139787 22.41746142014357 96-97 21.664354841232335 27.190256537487606 28.73967537125422 22.405713250025837 98-99 21.635971690707485 27.148648317142577 28.48806544990678 22.727314542243153 100-101 21.92932492204616 26.745989464908877 28.855556254960874 22.469129358084096 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3315.0 1 2164.0 2 1466.0 3 1904.0 4 1475.5 5 1327.0 6 2675.0 7 5777.5 8 9417.0 9 10513.5 10 7874.0 11 5171.5 12 4625.5 13 4685.5 14 4755.0 15 4698.0 16 4647.5 17 4614.5 18 4645.5 19 4841.5 20 5084.0 21 5521.0 22 6271.0 23 7223.5 24 8371.5 25 9768.0 26 11813.5 27 14432.0 28 16069.5 29 17972.5 30 20926.0 31 23115.5 32 25501.5 33 27854.0 34 30342.5 35 32498.5 36 34862.5 37 38066.0 38 39831.0 39 41006.5 40 42844.5 41 46070.0 42 49021.0 43 51684.0 44 54136.0 45 55524.0 46 55460.5 47 55950.5 48 59795.5 49 60154.0 50 57455.5 51 54651.0 52 52664.5 53 52358.5 54 50611.5 55 46913.0 56 41984.0 57 38859.0 58 36762.0 59 33560.5 60 29207.5 61 23950.0 62 18415.0 63 13304.5 64 9889.0 65 7771.5 66 6176.5 67 5151.5 68 4030.0 69 2738.5 70 1949.0 71 1585.5 72 1288.5 73 1062.5 74 804.5 75 664.5 76 467.5 77 274.0 78 177.5 79 106.5 80 63.5 81 38.0 82 25.0 83 21.5 84 14.0 85 9.0 86 5.5 87 3.5 88 3.0 89 3.5 90 2.5 91 1.5 92 0.5 93 2.5 94 2.0 95 0.5 96 1.0 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.49830241218094357 2 0.052478698920630874 3 0.06081643613231989 4 0.06958332158284584 5 0.08521657885476275 6 0.11268206614032657 7 0.073261735058591 8 0.05309176783325506 9 0.052907847159467815 10-11 0.07954569141298896 12-13 0.10998456292478014 14-15 0.05922245695949699 16-17 0.06424962204301536 18-19 0.062134534294461904 20-21 0.07933111729357051 22-23 0.06851045098575351 24-25 0.09287994026256516 26-27 0.07034965772362609 28-29 0.07148383521198083 30-31 0.07494767456830753 32-33 0.07405872464500245 34-35 0.07620446583918712 36-37 0.07154514210324325 38-39 0.04426357549146669 40-41 0.046562583913807416 42-43 0.04438618927399154 44-45 0.022990084223407217 46-47 0.02905946645838672 48-49 0.02942730780596124 50-51 0.027618754513719868 52-53 0.027588101068088657 54-55 0.027342873503038982 56-57 0.027158952829251723 58-59 0.023265965234088103 60-61 0.03102128697878414 62-63 0.022070480854470928 64-65 0.02430818238554923 66-67 0.017196582999108596 68-69 0.019434284530186897 70-71 0.04404900137204822 72-73 0.039297717299210735 74-75 0.03328964195549365 76-77 0.023480539353506573 78-79 0.016828741651534085 80-81 0.018300107041832145 82-83 0.0229287773321448 84-85 0.019740818986498997 86-87 0.009778449156355869 88-89 0.04426357549146669 90-91 0.055605350375014254 92-93 0.03546603659530953 94-95 0.05612645895074482 96-97 0.04613343567497048 98-99 0.03543538314967832 100-101 0.16985074224253252 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1631138.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.62465612423169 #Duplication Level Percentage of deduplicated Percentage of total 1 74.18632738622588 34.58912003502454 2 13.511226575003258 12.599125857522154 3 4.783088731423205 6.690296019428837 4 2.241752969293228 4.1808384523508835 5 1.256467154048949 2.929117449446215 6 0.794197112017391 2.221750034560127 7 0.5484422387609674 1.789965155036372 8 0.3881348820735133 1.4477324325197425 9 0.2847128443531494 1.1947174615905745 >10 1.7619068495031924 15.567412079307264 >50 0.15659526812192384 5.019518148188908 >100 0.0818743955620547 6.831487054126644 >500 0.0034278358486913136 1.024641299545024 >1k 0.001450238243677094 1.25054307984546 >5k 1.318398403342813E-4 0.5791691357797839 >10k+ 2.636796806685626E-4 2.0845663057274497 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18900 1.1587002448597237 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15012 0.9203390516314377 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9422 0.5776335294745141 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3415 0.2093630336611617 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2997 0.18373675311347046 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2898 0.17766737087849097 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1666 0.10213728084319046 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 6.130689126241924E-5 9 0.0 0.0 0.0 0.0 6.130689126241924E-5 10-11 0.0 0.0 0.0 0.0 6.130689126241924E-5 12-13 0.0 0.0 0.0 0.0 6.130689126241924E-5 14-15 0.0 0.0 0.0 0.0 6.130689126241924E-5 16-17 0.0 0.0 0.0 0.0 6.130689126241924E-5 18-19 0.0 0.0 0.0 2.4522756504967696E-4 6.130689126241924E-5 20-21 0.0 0.0 0.0 3.371879019433058E-4 6.130689126241924E-5 22-23 0.0 0.0 0.0 7.663361407802405E-4 6.130689126241924E-5 24-25 0.0 0.0 0.0 0.0014713653902980618 9.196033689362886E-5 26-27 0.0 0.0 0.0 0.0030346911174897523 1.2261378252483848E-4 28-29 0.0 0.0 0.0 0.00812316309227055 1.2261378252483848E-4 30-31 0.0 0.0 0.0 0.022285054973889395 1.2261378252483848E-4 32-33 0.0 0.0 0.0 0.04919878023809145 1.2261378252483848E-4 34-35 0.0 0.0 0.0 0.08892564577613911 1.2261378252483848E-4 36-37 0.0 0.0 0.0 0.15780393810946713 1.2261378252483848E-4 38-39 0.0 0.0 0.0 0.255465815890501 1.2261378252483848E-4 40-41 0.0 0.0 0.0 0.3607910550793373 1.2261378252483848E-4 42-43 0.0 0.0 0.0 0.46311225659631494 1.2261378252483848E-4 44-45 0.0 0.0 0.0 0.588883344021168 1.2261378252483848E-4 46-47 0.0 0.0 0.0 0.7286937095451151 1.2261378252483848E-4 48-49 0.0 0.0 0.0 0.8769950795089072 1.2261378252483848E-4 50-51 0.0 0.0 0.0 1.0309673369144732 1.2261378252483848E-4 52-53 0.0 0.0 0.0 1.1817516359743934 1.2261378252483848E-4 54-55 0.0 0.0 0.0 1.343264641005237 1.2261378252483848E-4 56-57 0.0 0.0 0.0 1.5238134357730615 1.2261378252483848E-4 58-59 0.0 0.0 0.0 1.7216507738768885 1.2261378252483848E-4 60-61 0.0 0.0 0.0 1.9305233524079508 1.2261378252483848E-4 62-63 0.0 0.0 0.0 2.1357788243545306 1.2261378252483848E-4 64-65 0.0 0.0 0.0 2.3469810647535647 1.2261378252483848E-4 66-67 0.0 0.0 0.0 2.582951289222616 1.2261378252483848E-4 68-69 0.0 0.0 0.0 2.8362407104733016 1.2261378252483848E-4 70-71 0.0 0.0 0.0 3.115033798489153 1.2261378252483848E-4 72-73 0.0 0.0 0.0 3.3926927090166497 1.2261378252483848E-4 74-75 0.0 0.0 0.0 3.663270673603337 1.2261378252483848E-4 76-77 0.0 0.0 0.0 3.953650764067786 1.2261378252483848E-4 78-79 0.0 0.0 0.0 4.268032502461471 1.2261378252483848E-4 80-81 0.0 0.0 0.0 4.603595771786323 1.2261378252483848E-4 82-83 0.0 0.0 0.0 4.947098283529659 1.2261378252483848E-4 84-85 0.0 0.0 0.0 5.283121354538978 1.2261378252483848E-4 86-87 0.0 0.0 0.0 5.6293520229434915 1.2261378252483848E-4 88-89 0.0 0.0 0.0 5.995905925801496 1.8392067378725772E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6680 0.0 53.397762 1 GTATCAA 12045 0.0 43.13214 1 TATCAAC 15645 0.0 33.07653 2 ATCAACG 15790 0.0 32.712605 3 TCAACGC 15975 0.0 32.39326 4 CAACGCA 16295 0.0 31.669645 5 AACGCAG 16660 0.0 31.089891 6 ACGCAGA 18975 0.0 27.209196 7 CGCAGAG 18985 0.0 27.06966 8 TGGTATC 2925 0.0 25.668407 2 GCAGAGT 19845 0.0 25.344803 9 GAGTACT 10870 0.0 24.84009 12-13 GTGGTAT 3135 0.0 24.218014 1 GTACATG 12270 0.0 23.58343 1 GTACTTT 11450 0.0 23.381346 14-15 TACATGG 12060 0.0 23.207891 2 AGTACTT 11005 0.0 22.872322 12-13 GTAAGGT 560 0.0 22.062416 4 CAGAGTA 19335 0.0 21.858076 10-11 ACATGGG 12405 0.0 21.528175 3 >>END_MODULE