FastQCFastQC Report
Thu 26 May 2016
SRR938157_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938157_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1631138
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180851.1087351284808522No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149050.9137792142663588No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97920.6003170792416093No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34860.2137158229407935No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30750.18851869063193918No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28000.17165929553477388No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70400.050.2691231
GTATCAA122350.040.7664641
CGTATAG550.002579239234.547371
ATCAACG157250.031.5346243
TATCAAC157750.031.4375612
TCAACGC159700.031.109374
CAACGCA163500.030.3863375
AACGCAG167000.029.8063816
TGGTATC32300.026.7661912
ACGCAGA188750.026.3969047
CGCAGAG188350.026.2512258
GTGGTAT34900.025.4527021
GTACGGG950.001226378525.0013871
GCAGAGT196550.024.9385459
GAGTACT110350.024.2326312-13
GTACATG118150.023.8417821
TACATGG117850.023.620322
AGTACTT113150.023.02430512-13
GTACTTT117400.022.6560614-15
TATAACG1050.002195904522.6203042
Produced by FastQC (version 0.11.5)