FastQCFastQC Report
Thu 26 May 2016
SRR938156_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938156_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1669049
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185871.1136281798796799No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150590.9022503233877495No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92430.553788414839828No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33210.1989755842998019No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29660.17770598706209345No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29210.17500984093336985No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69300.054.63641
GTATCAA122450.042.7062721
TATCAAC157350.033.187172
ATCAACG157200.033.037313
TCAACGC158700.032.7849854
CAACGCA160650.032.3870355
AACGCAG165400.031.4503216
ACGCAGA187400.027.4250377
CGCAGAG187600.027.2658088
GCAGAGT194650.025.9119429
GAGTACT109050.025.19503812-13
GTACATG125050.024.9462781
TACATGG122800.024.9414732
GTACACG1751.4492616E-724.4935991
GTACTTT116450.023.36771614-15
TGGTATC29150.023.3309292
ACATGGG125600.023.1737543
GTGTAAG7850.022.4481321
CAGAGTA191900.022.42917410-11
AGTACTT112300.022.0954912-13
Produced by FastQC (version 0.11.5)