##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938156_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1669049 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.02843954850936 33.0 31.0 34.0 30.0 34.0 2 32.127135871984585 33.0 31.0 34.0 30.0 34.0 3 32.12568774194167 34.0 31.0 34.0 30.0 34.0 4 35.662840935167274 37.0 35.0 37.0 35.0 37.0 5 35.68816254046466 37.0 35.0 37.0 35.0 37.0 6 35.736113798935804 37.0 36.0 37.0 35.0 37.0 7 35.67737615851901 37.0 35.0 37.0 35.0 37.0 8 35.67686029589305 37.0 35.0 37.0 35.0 37.0 9 37.30973686212927 39.0 37.0 39.0 34.0 39.0 10-11 37.225565276993066 39.0 37.0 39.0 34.0 39.0 12-13 37.072106930353755 39.0 37.0 39.0 33.5 39.0 14-15 38.22187485208643 40.0 38.0 41.0 33.5 41.0 16-17 38.06124295931396 40.0 38.0 41.0 33.0 41.0 18-19 38.301367425402134 40.0 38.0 41.0 34.0 41.0 20-21 38.33544042146156 40.0 38.0 41.0 34.0 41.0 22-23 38.223496134625165 40.0 38.0 41.0 34.0 41.0 24-25 38.10553375005767 40.0 38.0 41.0 33.0 41.0 26-27 37.92681131590504 40.0 38.0 41.0 33.0 41.0 28-29 37.747720708019955 40.0 38.0 41.0 33.0 41.0 30-31 37.474085242554295 40.0 37.0 41.0 32.5 41.0 32-33 37.13379415463537 40.0 36.0 41.0 31.5 41.0 34-35 36.8827637774565 39.0 35.0 41.0 31.0 41.0 36-37 36.62340021173735 39.0 35.0 41.0 30.5 41.0 38-39 36.331469896929335 39.0 35.0 40.5 30.0 41.0 40-41 36.1693952664062 39.0 35.0 41.0 30.0 41.0 42-43 35.91157719156238 39.0 35.0 41.0 29.5 41.0 44-45 35.50427968262166 39.0 35.0 40.0 27.5 41.0 46-47 34.995306009589896 38.0 35.0 40.0 25.0 41.0 48-49 34.50159671765179 38.0 34.0 40.0 23.0 41.0 50-51 33.74426514739831 37.5 33.5 39.5 21.5 40.5 52-53 33.80600060273845 38.0 33.5 40.0 22.0 40.5 54-55 33.88844305949077 38.0 34.0 40.0 20.5 41.0 56-57 33.60219022928626 38.0 33.5 40.0 18.5 41.0 58-59 33.22913347660854 37.5 33.0 40.0 15.5 41.0 60-61 32.7542972075715 37.0 32.5 40.0 10.5 41.0 62-63 32.24045938735172 36.0 31.5 39.0 7.5 41.0 64-65 31.74037880253965 35.5 31.0 39.0 4.0 40.5 66-67 31.206475663686327 35.0 30.5 39.0 2.0 40.0 68-69 30.573674289969915 35.0 29.5 38.0 2.0 40.0 70-71 30.028243928129132 35.0 29.0 37.5 2.0 40.0 72-73 29.362130470705175 34.0 28.5 37.0 2.0 39.0 74-75 28.728449254635425 34.0 27.5 36.0 2.0 39.0 76-77 28.005551664450834 34.0 26.0 35.5 2.0 38.5 78-79 27.268070020712393 33.0 25.5 35.0 2.0 37.0 80-81 26.566752384142106 32.5 23.5 35.0 2.0 37.0 82-83 25.86401807256707 32.0 21.0 35.0 2.0 36.0 84-85 25.143731550122254 31.5 19.0 35.0 2.0 36.0 86-87 24.401625117057677 31.0 13.0 34.0 2.0 35.0 88-89 23.55405922773987 31.0 4.5 34.0 2.0 35.0 90-91 22.67484717345027 30.0 2.0 34.0 2.0 35.0 92-93 21.752738835109096 29.0 2.0 34.0 2.0 35.0 94-95 20.677124817785455 29.0 2.0 34.0 2.0 35.0 96-97 19.410107492350434 26.5 2.0 33.5 2.0 35.0 98-99 17.859939402617897 24.0 2.0 33.0 2.0 35.0 100-101 13.8546435724775 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2692510718091796 1101 2 0.2633526941888036 1101 3 0.1911929826808887 1101 4 0.21651687669954356 1101 5 0.22423606921869776 1101 6 0.1744200381980079 1101 7 0.24429654650966626 1101 8 0.3301739441431337 1101 9 0.3461607177895445 1101 10-11 0.4224156969998134 1101 12-13 0.40186196811863084 1101 14-15 0.517249945074461 1101 16-17 0.4479897614466779 1101 18-19 0.5378056147736103 1101 20-21 0.6430177750807289 1101 22-23 0.6240861841277692 1101 24-25 0.6410091617779372 1101 26-27 0.6255031920219736 1101 28-29 0.6538335583081007 1101 30-31 0.6986356101535804 1101 32-33 0.6003451270406615 1101 34-35 0.6481143863655134 1101 36-37 0.6342611760406172 1101 38-39 0.6934840132397966 1101 40-41 0.7239814763285821 1101 42-43 0.8141807441929743 1101 44-45 1.002277148696102 1101 46-47 0.8828655524619222 1101 48-49 0.9454519244203965 1101 50-51 1.0601360165491869 1101 52-53 0.9895303536201325 1101 54-55 0.9047724254854828 1101 56-57 0.9983456947558409 1101 58-59 1.0758260637254864 1101 60-61 0.9868583515129004 1101 62-63 0.8589578331107148 1101 64-65 0.9574887714016249 1101 66-67 0.9312143389325982 1101 68-69 0.7655121711243211 1101 70-71 0.7660098790600713 1101 72-73 0.6651175010631079 1101 74-75 0.6524611075528135 1101 76-77 0.3860158121504291 1101 78-79 0.19060762047100255 1101 80-81 0.5033093581526487 1101 82-83 0.36860810070106353 1101 84-85 0.4505523611981026 1101 86-87 0.2859401515461393 1101 88-89 0.18197480863711846 1101 90-91 0.3718030955682181 1101 92-93 0.28896467156664585 1101 94-95 0.02217355944776145 1101 96-97 -0.31336221045365065 1101 98-99 -0.3977432824040257 1101 100-101 -0.9148267537132693 1102 1 0.25535688289785696 1102 2 0.23741258341318883 1102 3 0.23719079680755328 1102 4 0.1829864134055441 1102 5 0.19417614294560792 1102 6 0.18549852587005944 1102 7 0.29449614638370036 1102 8 0.21563464473405958 1102 9 0.2872472635539509 1102 10-11 0.31637027529298223 1102 12-13 0.3953846974964037 1102 14-15 0.40293062232470334 1102 16-17 0.3990662577510733 1102 18-19 0.32556148236155025 1102 20-21 0.3758269464865407 1102 22-23 0.4174929047620495 1102 24-25 0.3807779315758353 1102 26-27 0.27717501463279604 1102 28-29 0.31325203264261603 1102 30-31 0.3555250475690599 1102 32-33 0.290266954282572 1102 34-35 0.3382267828607084 1102 36-37 0.29098482052128816 1102 38-39 0.1683742564645172 1102 40-41 0.2912308881804577 1102 42-43 0.35071933041455594 1102 44-45 0.24264471811610377 1102 46-47 0.36980426464909044 1102 48-49 0.1458018030859023 1102 50-51 0.299887188214349 1102 52-53 0.19478084914650395 1102 54-55 -8.024777247825909E-4 1102 56-57 0.09013222940563992 1102 58-59 0.007743750775112801 1102 60-61 -0.01756433711129546 1102 62-63 0.12390443667108997 1102 64-65 0.15207225138606262 1102 66-67 0.09743912266080912 1102 68-69 -0.07439854150743486 1102 70-71 -0.2585448418705951 1102 72-73 -0.25571243613934413 1102 74-75 -0.24881244152745197 1102 76-77 -0.4781490638290471 1102 78-79 -0.5563400297151659 1102 80-81 -0.6383574628836044 1102 82-83 -0.45519777337040424 1102 84-85 -0.3873813401523236 1102 86-87 -0.6438106496320088 1102 88-89 -0.615662178933043 1102 90-91 -0.5089193077809853 1102 92-93 -0.6914733830060911 1102 94-95 -0.8860262441941238 1102 96-97 -1.0143405277016733 1102 98-99 -0.9507238503606068 1102 100-101 -1.5484262202119812 1103 1 0.1394758582700959 1103 2 0.19405114103086163 1103 3 0.24141068248957964 1103 4 0.16054798280784155 1103 5 0.1898063031319026 1103 6 0.1332015814060128 1103 7 0.16107401668219978 1103 8 0.13121752526472363 1103 9 0.08092978419470143 1103 10-11 0.2647895823284827 1103 12-13 0.03515165113410745 1103 14-15 0.09767517105097312 1103 16-17 0.1845092398977073 1103 18-19 0.21633489284775465 1103 20-21 0.24203875228712235 1103 22-23 0.25922425752472833 1103 24-25 0.2094729777969988 1103 26-27 0.16316400623536254 1103 28-29 0.12380968559583039 1103 30-31 0.2629565979183681 1103 32-33 0.20245734310481112 1103 34-35 0.019081168578019003 1103 36-37 -0.056186338888778664 1103 38-39 -0.04462235633626932 1103 40-41 0.004058141020763628 1103 42-43 0.015166969960112908 1103 44-45 0.12394543789593371 1103 46-47 0.11382285896142008 1103 48-49 0.2217729766092873 1103 50-51 0.21294642891719207 1103 52-53 0.010503980894441156 1103 54-55 0.010553460458829989 1103 56-57 -0.011908028868290899 1103 58-59 0.19187066489549665 1103 60-61 -0.12083862968062675 1103 62-63 -0.049321694159466745 1103 64-65 -0.21934825528248325 1103 66-67 -0.4338880596128476 1103 68-69 -0.4133530965847072 1103 70-71 -0.4738692720448938 1103 72-73 -0.5766133958457864 1103 74-75 -0.5522384523947146 1103 76-77 -0.5182759073827796 1103 78-79 -0.7044012110008921 1103 80-81 -0.7659366739484526 1103 82-83 -0.8462366908779906 1103 84-85 -1.0443145135345837 1103 86-87 -1.064870384301404 1103 88-89 -1.0597478115630636 1103 90-91 -1.043690142584456 1103 92-93 -0.8492930499315001 1103 94-95 -0.8179329550825578 1103 96-97 -1.170697700801668 1103 98-99 -1.2214104638670626 1103 100-101 -1.336826827085174 1104 1 0.2667271378408884 1104 2 0.23049361340348185 1104 3 0.33284467374687665 1104 4 0.2010636115867115 1104 5 0.2697381416172462 1104 6 0.3612567983201984 1104 7 0.2461212592091968 1104 8 0.30549950959892413 1104 9 0.3779798203073028 1104 10-11 0.2859874349857563 1104 12-13 0.3870375270745612 1104 14-15 0.3873206834633507 1104 16-17 0.4000836030737105 1104 18-19 0.46474087914964457 1104 20-21 0.38741029259256976 1104 22-23 0.5356884632695156 1104 24-25 0.5424643107716705 1104 26-27 0.4870031792813734 1104 28-29 0.3556439756252985 1104 30-31 0.37019725758626265 1104 32-33 0.41568376996403344 1104 34-35 0.39105231633369186 1104 36-37 0.26884356300668344 1104 38-39 0.2356182490393266 1104 40-41 0.3111098881634291 1104 42-43 0.2996240716397196 1104 44-45 0.3477697628205405 1104 46-47 0.013665493552849739 1104 48-49 0.15973106788945302 1104 50-51 0.17652698964148072 1104 52-53 0.11957967916745105 1104 54-55 0.18092660485495315 1104 56-57 0.1654636808949448 1104 58-59 0.2520341423008503 1104 60-61 0.17781961016301295 1104 62-63 0.3475251982644423 1104 64-65 0.021652796013867714 1104 66-67 0.17639461406903933 1104 68-69 0.12577005809405506 1104 70-71 0.14071668335974152 1104 72-73 0.09092770589304777 1104 74-75 0.14817745952672112 1104 76-77 -0.15259834770649405 1104 78-79 -0.15724135637263714 1104 80-81 0.08087821892296887 1104 82-83 0.06042600257945807 1104 84-85 0.052761969508946294 1104 86-87 0.04091384329191783 1104 88-89 -0.1981629452948681 1104 90-91 -0.3394386030687109 1104 92-93 -0.2064500089704282 1104 94-95 -0.48100316818806377 1104 96-97 -1.0857039279639409 1104 98-99 -1.5932645952642979 1104 100-101 -1.348681674153637 1105 1 0.02106767565782519 1105 2 0.08537899244517178 1105 3 0.1918126317572586 1105 4 0.0345260678140491 1105 5 0.09826752941404493 1105 6 0.07029069508845254 1105 7 0.02243679194729964 1105 8 0.06263369296261345 1105 9 0.1099424997737799 1105 10-11 0.09220266505980135 1105 12-13 0.2562802241706166 1105 14-15 0.08041794571366978 1105 16-17 0.11670992600949148 1105 18-19 0.19461865428758074 1105 20-21 0.05954201401308268 1105 22-23 0.21215354901285366 1105 24-25 -0.026878700466035355 1105 26-27 -0.03518816822219861 1105 28-29 0.05479624078518697 1105 30-31 0.01859446944995824 1105 32-33 0.03783173217522062 1105 34-35 0.10584050313836713 1105 36-37 0.07771642917911947 1105 38-39 0.13557096136613467 1105 40-41 0.2256288106931379 1105 42-43 0.31355901436079847 1105 44-45 0.31544025955918187 1105 46-47 0.28966080151382556 1105 48-49 0.2081619090935476 1105 50-51 0.22460145165811696 1105 52-53 0.2170984536362468 1105 54-55 0.19870768817882123 1105 56-57 0.12206805867754156 1105 58-59 0.19151338106113514 1105 60-61 0.2357504530887411 1105 62-63 0.10441835222599849 1105 64-65 0.07809499544159237 1105 66-67 3.409999479302428E-4 1105 68-69 0.09296882585993416 1105 70-71 -0.3898320717233972 1105 72-73 -0.45377750940933836 1105 74-75 -0.362395696913989 1105 76-77 -0.4082633994761693 1105 78-79 -0.5542449018003666 1105 80-81 -0.4877675286871117 1105 82-83 -0.6427761304159745 1105 84-85 -0.5910723262203597 1105 86-87 -0.5895582414861558 1105 88-89 -0.6265519621588922 1105 90-91 -0.7532786261157121 1105 92-93 -0.8678163243080483 1105 94-95 -0.8423423964119188 1105 96-97 -1.3432782769263092 1105 98-99 -1.5765767524430139 1105 100-101 -1.4803778439668847 1106 1 0.19057360551915536 1106 2 0.2891629632170023 1106 3 0.23694884771064295 1106 4 0.3025434965122429 1106 5 0.19904841632933312 1106 6 0.2186971304427061 1106 7 0.3261075461248737 1106 8 0.17850436733660757 1106 9 0.36848167284179567 1106 10-11 0.4196084544853633 1106 12-13 0.3425795415423849 1106 14-15 0.42001147833685337 1106 16-17 0.012373692527972935 1106 18-19 0.49445554991974205 1106 20-21 0.5510334941167514 1106 22-23 0.6415391751823663 1106 24-25 0.5114714720976181 1106 26-27 0.46055367434733085 1106 28-29 0.44166581248769177 1106 30-31 0.3628887870892825 1106 32-33 0.221143597754633 1106 34-35 0.2587322032707178 1106 36-37 0.2936042946102262 1106 38-39 0.33014254800379916 1106 40-41 0.2757634950413177 1106 42-43 0.21256850609960765 1106 44-45 0.18340493530761393 1106 46-47 0.39530574635286087 1106 48-49 0.2895971685415688 1106 50-51 0.13994265832706532 1106 52-53 0.1554742021108524 1106 54-55 0.20917464026759802 1106 56-57 0.016134489472790392 1106 58-59 0.10155927865552883 1106 60-61 0.37585145897625694 1106 62-63 0.2464143772153875 1106 64-65 -0.03129890603089436 1106 66-67 -0.18413968897525734 1106 68-69 -0.23950347989481457 1106 70-71 -0.20959774244639462 1106 72-73 -0.20655199012775327 1106 74-75 -0.043304817317537214 1106 76-77 -0.4134504217449084 1106 78-79 -0.21898937232634097 1106 80-81 -0.24577348404008248 1106 82-83 -0.21244710285255763 1106 84-85 -0.195888476712895 1106 86-87 -0.27406905689423056 1106 88-89 -0.48155087297059396 1106 90-91 -0.39514297940542065 1106 92-93 -0.273215369614487 1106 94-95 -0.9510777287112582 1106 96-97 -1.0030432397822473 1106 98-99 -1.2173658219644103 1106 100-101 -1.4599478737183684 1107 1 0.0941942050685114 1107 2 0.06634530401257877 1107 3 -0.009977583694158909 1107 4 0.175848214600677 1107 5 0.04928958972572417 1107 6 0.0742372646087972 1107 7 0.08488274510363425 1107 8 0.14388334798276503 1107 9 0.04072574203243562 1107 10-11 0.08542266101203211 1107 12-13 0.09920859715362695 1107 14-15 0.05088767028175312 1107 16-17 0.131240477425294 1107 18-19 0.14289927469934582 1107 20-21 0.18784692100651768 1107 22-23 0.16907706467953432 1107 24-25 0.20145656475447282 1107 26-27 0.24757571836682501 1107 28-29 0.22153190342248763 1107 30-31 0.18982866937268028 1107 32-33 0.2184769824926036 1107 34-35 0.21931127644520387 1107 36-37 0.2805967809665759 1107 38-39 0.18811369287062263 1107 40-41 0.3206868593030947 1107 42-43 0.39549402935592326 1107 44-45 0.44952242916047425 1107 46-47 0.37264915499397944 1107 48-49 0.2945448643289552 1107 50-51 0.10944257693640225 1107 52-53 0.2797191965848569 1107 54-55 0.15352912660681994 1107 56-57 0.04759840770515211 1107 58-59 0.08476281529417662 1107 60-61 0.07503583882221676 1107 62-63 0.16919176945842906 1107 64-65 0.11683853281901335 1107 66-67 0.168189797578151 1107 68-69 0.1704099033010138 1107 70-71 0.05989746407171026 1107 72-73 -0.08600598726622977 1107 74-75 -0.10889070160571279 1107 76-77 -0.3560978459444968 1107 78-79 -0.33172829677010185 1107 80-81 -0.32390209842823836 1107 82-83 -0.43147367047963314 1107 84-85 -0.5317127928171104 1107 86-87 -0.27811502976972236 1107 88-89 -0.4903539161247821 1107 90-91 -0.5632361245978004 1107 92-93 -0.718587239286613 1107 94-95 -0.9317795732807888 1107 96-97 -0.8629099304377448 1107 98-99 -1.189251772221862 1107 100-101 -1.3831579216103496 1108 1 -0.15297996174188455 1108 2 -0.04937637703946507 1108 3 -0.07723766283248779 1108 4 -0.045113568536308435 1108 5 0.05177109870314922 1108 6 -0.00297134712097602 1108 7 0.031483746455933215 1108 8 -0.046393586169450884 1108 9 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-0.7965067117480302 2316 46-47 -0.6679811758874621 2316 48-49 -0.6555493819248142 2316 50-51 -0.7106606380461855 2316 52-53 -0.76233104487455 2316 54-55 -0.5787916190078732 2316 56-57 -0.5846580150485607 2316 58-59 -0.5005089073482978 2316 60-61 -0.7216143989900132 2316 62-63 -0.4823308421823498 2316 64-65 -0.3270393565290881 2316 66-67 -0.3370501117730349 2316 68-69 -0.3334494683574718 2316 70-71 -0.4315246348097652 2316 72-73 -0.365181709696504 2316 74-75 -0.5483579989016647 2316 76-77 -0.6265493659036494 2316 78-79 -0.4863402293239325 2316 80-81 -0.3086051108269672 2316 82-83 -0.24734699423084905 2316 84-85 -0.4078602015839188 2316 86-87 -0.38467339199032224 2316 88-89 -0.6351002469863261 2316 90-91 -0.34544521699891817 2316 92-93 -0.339488107479454 2316 94-95 -0.5218846284276957 2316 96-97 -0.5390592456811021 2316 98-99 -0.7241419508835811 2316 100-101 -0.33139293877692744 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5337.0 3 1373.0 4 1262.0 5 1418.0 6 1633.0 7 2177.0 8 2536.0 9 3445.0 10 4220.0 11 5666.0 12 7637.0 13 9823.0 14 11659.0 15 15251.0 16 19316.0 17 26417.0 18 24740.0 19 17350.0 20 17191.0 21 17731.0 22 19290.0 23 21708.0 24 24087.0 25 27457.0 26 31324.0 27 36024.0 28 41291.0 29 48669.0 30 57502.0 31 68670.0 32 84393.0 33 103966.0 34 137010.0 35 185626.0 36 220919.0 37 235232.0 38 122575.0 39 7124.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.99623185831787 19.130279298501623 14.84389813056971 21.029590712610794 2 15.515681733847837 20.823054987228375 40.40401618318725 23.257247095736535 3 18.830296782308036 26.57347189335087 30.368670618323478 24.227560706017613 4 12.36525466029296 17.68577578759431 37.223010136497145 32.72595941561558 5 13.041137008299442 38.24976362353859 33.2067055874893 15.50239378067266 6 29.734866676912436 36.943726706881314 17.77625158489261 15.545155031313637 7 27.186529825195244 35.09778491819099 20.24097094149229 17.474714315121478 8 26.868676694019445 33.85820445390979 19.610897461264223 19.662221390806543 9 27.771805509897035 16.28691441446846 20.2594657113171 35.681814364317404 10-11 25.00886145718941 27.569447734795627 25.902504178829304 21.51918662918565 12-13 26.39399326417486 25.417343624594015 27.207812957087242 20.98085015414388 14-15 23.34129809040824 25.840311870487948 26.946288138211784 23.872101900892023 16-17 21.048392370637597 29.50102323594543 27.387925515862705 22.062658877554263 18-19 21.014574268474316 26.65457768818535 31.62776745365017 20.70308058969017 20-21 22.43648046268021 25.650824029133496 31.637049213767614 20.27564629441868 22-23 21.923548291916564 25.77885128833774 31.550684803218694 20.746915616527005 24-25 21.221198592476576 26.135852249068357 31.840939405702322 20.80200975275274 26-27 20.955934192596878 26.517156570090023 31.685638186486308 20.841271050826794 28-29 20.830912513028938 26.423599811033544 32.03179435650172 20.713693319435798 30-31 20.95842589083093 26.291836788546703 31.858030792260195 20.891706528362175 32-33 20.85395935663604 26.386692645657917 31.505595721144964 21.25375227656108 34-35 21.30173824366955 26.429273713333572 31.366796070683556 20.902191972313318 36-37 21.34085461762041 26.170183548679766 31.27565402971775 21.213307803982076 38-39 20.991139694653512 26.3375955875014 31.397270243059687 21.2739944747854 40-41 21.04775813559759 26.397159727555426 31.44617325206279 21.108908884784196 42-43 21.205248343030835 26.282723454769464 31.072301111241014 21.439727090958687 44-45 21.04547356256526 26.61662333054016 30.91903756636781 21.41886554052677 46-47 21.309203847457674 26.93397211849057 30.227588193384776 21.52923584066698 48-49 21.299821947536113 26.89737063174309 30.10549181411215 21.69731560660865 50-51 21.513056713624536 27.245715477812983 29.703742491951303 21.53748531661118 52-53 21.595137359583134 27.34977527009092 29.476671030713035 21.578416339612904 54-55 21.17077179832102 27.4256124726944 29.576700260647087 21.826915468337493 56-57 21.279293290242737 27.650408806303574 29.428270487885637 21.64202741556805 58-59 21.35933155313427 27.033588693687765 29.609142498151414 21.997937255026553 60-61 21.343213132440756 27.220265076308714 29.807943298544252 21.62857849270628 62-63 21.16984613503214 27.36311041386541 29.67740929927806 21.789634151824387 64-65 21.001853041214357 27.19391178708637 29.779897784532334 22.024337387166934 66-67 21.08560237235766 27.30665779579923 29.66277217604476 21.944967655798354 68-69 21.204675890743996 27.158417607585505 29.51099200050362 22.125914501166875 70-71 21.096375737538832 27.50000374670477 29.42152376984442 21.98209674591197 72-73 21.308397545106097 27.432833906213844 29.116077540885925 22.14269100779413 74-75 21.172808203741265 27.800105735617798 28.87616345144074 22.150922609200197 76-77 21.373367615786428 27.452760000155813 29.023516400474264 22.150355983583495 78-79 21.406228697193384 27.605530826612572 28.8444983137541 22.14374216243994 80-81 21.193402813153618 28.0251589665526 28.844982889745236 21.936455330548547 82-83 21.484047969057787 27.896112986535893 28.49632549250626 22.12351355190006 84-85 21.476620268965636 27.508181572479952 28.617705276417592 22.397492882136817 86-87 21.63316295312117 27.425580763996138 28.588756823748472 22.35249945913422 88-89 21.610856554446897 27.31276595425774 28.695495770961955 22.380881720333406 90-91 21.478563303311933 27.65472878320801 28.623928929921107 22.242778983558956 92-93 21.689592727621605 27.183692315072438 28.58402091528351 22.54269404202245 94-95 21.527798805416907 27.221090810320437 28.610094206155072 22.641016178107584 96-97 21.738907556702173 27.055102408502652 28.485550872697374 22.720439162097808 98-99 21.703988506549894 27.01719131660612 28.234158962951728 23.044661213892255 100-101 22.462471758581014 26.068824473056818 28.405618167616346 23.063085600745826 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3196.0 1 2109.5 2 1399.0 3 1844.5 4 1511.5 5 1356.0 6 2653.0 7 5591.5 8 8925.5 9 10140.5 10 7709.5 11 5045.5 12 4603.5 13 4616.0 14 4691.5 15 4755.0 16 4777.0 17 4810.5 18 4872.5 19 4997.5 20 5256.0 21 5753.5 22 6515.5 23 7529.0 24 8751.5 25 10263.0 26 12297.5 27 14717.0 28 16585.0 29 18687.0 30 21589.0 31 23671.0 32 26048.5 33 28632.0 34 31345.0 35 33444.0 36 35686.5 37 39009.0 38 40934.0 39 41900.0 40 43615.5 41 46871.0 42 49813.0 43 52260.0 44 54359.0 45 56134.5 46 56299.5 47 56495.0 48 60102.0 49 61059.5 50 58701.0 51 55920.0 52 53906.0 53 53513.0 54 52016.0 55 48101.5 56 43129.5 57 39934.0 58 37739.0 59 34320.0 60 29993.0 61 25133.0 62 19726.5 63 14633.0 64 10869.0 65 8485.5 66 6711.0 67 5558.0 68 4372.5 69 3027.5 70 2173.5 71 1668.0 72 1397.0 73 1190.0 74 950.5 75 804.5 76 574.0 77 328.5 78 193.5 79 131.0 80 73.0 81 46.0 82 33.0 83 17.0 84 12.5 85 9.5 86 6.0 87 4.5 88 4.5 89 4.5 90 3.0 91 2.0 92 1.0 93 1.0 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14649060632731573 2 0.053803093857639886 3 0.15427947292140615 4 0.1318715028737922 5 0.1961596094542461 6 0.1989755842998019 7 0.16716105998086336 8 0.07255628804187295 9 0.12492143729752692 10-11 0.10281303904199338 12-13 0.15496848804319108 14-15 0.13969032664709063 16-17 0.13648490847183037 18-19 0.13429803438964344 20-21 0.13414824849360324 22-23 0.19642922406711846 24-25 0.21392421672461384 26-27 0.14547206223424236 28-29 0.12501130883515102 30-31 0.09688151755880145 32-33 0.123483492695541 34-35 0.09181875427264269 36-37 0.08543787510133016 38-39 0.05263476386852632 40-41 0.06231093275272326 42-43 0.06554630810719157 44-45 0.04164047909917564 46-47 0.052874421302190645 48-49 0.04316829523878568 50-51 0.05527099563883385 52-53 0.02938799280308727 54-55 0.05350352206555949 56-57 0.028249619993181745 58-59 0.05293433566060673 60-61 0.03963334809223695 62-63 0.053803093857639886 64-65 0.042569151654624875 66-67 0.03993291988431735 68-69 0.06779309654779458 70-71 0.05491150948833737 72-73 0.05218540618040573 74-75 0.044156882152651 76-77 0.02171895492582902 78-79 0.024055614904056142 80-81 0.02750069051298075 82-83 0.023366599782271222 84-85 0.02156916902978882 86-87 0.025643345402082265 88-89 0.06689438117155339 90-91 0.11081160589054007 92-93 0.08202275667161359 94-95 0.07612119236762971 96-97 0.0686318975656197 98-99 0.05802705612597353 100-101 0.1300740721213098 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1669049.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.75927695609709 #Duplication Level Percentage of deduplicated Percentage of total 1 74.4903284509687 34.831138985894846 2 13.236986967063332 12.379038793143241 3 4.756987691732564 6.67299914863404 4 2.2458759401666044 4.200621403811408 5 1.2618225162595123 2.9500954253608933 6 0.8047819833759119 2.257861418997083 7 0.5367012358637526 1.756703321130296 8 0.39089794041173515 1.4622484045824207 9 0.29345371167169476 1.2349515040066297 >10 1.7369570844205218 15.438468353330284 >50 0.15739348911347043 5.050342858789464 >100 0.08230078805090242 6.873860916165995 >500 0.0037175308404076807 1.1121463003015621 >1k 0.0014100979049822238 1.2087113369397975 >5k 1.281907186347476E-4 0.5540351543433945 >10k+ 2.563814372694952E-4 2.01677667456863 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18587 1.1136281798796799 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15059 0.9022503233877495 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9243 0.553788414839828 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3321 0.1989755842998019 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2966 0.17770598706209345 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2921 0.17500984093336985 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 5.9914358416080055E-5 0.0 3 0.0 0.0 0.0 5.9914358416080055E-5 0.0 4 0.0 0.0 0.0 5.9914358416080055E-5 0.0 5 0.0 0.0 0.0 5.9914358416080055E-5 0.0 6 0.0 0.0 0.0 5.9914358416080055E-5 0.0 7 0.0 0.0 0.0 5.9914358416080055E-5 0.0 8 0.0 0.0 0.0 1.1982871683216011E-4 0.0 9 0.0 0.0 0.0 1.1982871683216011E-4 0.0 10-11 0.0 0.0 0.0 1.1982871683216011E-4 0.0 12-13 0.0 0.0 0.0 1.4978589604020014E-4 0.0 14-15 0.0 0.0 0.0 1.7974307524824018E-4 0.0 16-17 0.0 0.0 0.0 2.097002544562802E-4 1.1982871683216011E-4 18-19 0.0 0.0 0.0 2.3965743366432022E-4 1.1982871683216011E-4 20-21 0.0 0.0 0.0 3.2952897128844035E-4 1.1982871683216011E-4 22-23 0.0 0.0 0.0 6.590579425768806E-4 1.1982871683216011E-4 24-25 0.0 0.0 0.0 0.001378030243569841 1.1982871683216011E-4 26-27 0.0 0.0 0.0 0.003385161250508523 1.1982871683216011E-4 28-29 0.0 0.0 0.0 0.009077025300036129 1.1982871683216011E-4 30-31 0.0 0.0 0.0 0.02534377361000186 1.1982871683216011E-4 32-33 0.0 0.0 0.0 0.05047784696554745 1.1982871683216011E-4 34-35 0.0 0.0 0.0 0.08882303635183869 1.1982871683216011E-4 36-37 0.0 0.0 0.0 0.15460900189269458 1.1982871683216011E-4 38-39 0.0 0.0 0.0 0.2525989350821935 1.1982871683216011E-4 40-41 0.0 0.0 0.0 0.35777859128162204 1.1982871683216011E-4 42-43 0.0 0.0 0.0 0.46047180160678325 1.1982871683216011E-4 44-45 0.0 0.0 0.0 0.5852134958290619 1.1982871683216011E-4 46-47 0.0 0.0 0.0 0.7252932658058571 1.1982871683216011E-4 48-49 0.0 0.0 0.0 0.87564834825101 1.1982871683216011E-4 50-51 0.0 0.0 0.0 1.0353500706090715 1.1982871683216011E-4 52-53 0.0 0.0 0.0 1.1862743394591773 1.1982871683216011E-4 54-55 0.0 0.0 0.0 1.345676490025158 1.1982871683216011E-4 56-57 0.0 0.0 0.0 1.5283853260149942 1.1982871683216011E-4 58-59 0.0 0.0 0.0 1.728888726454406 1.1982871683216011E-4 60-61 0.0 0.0 0.0 1.9457787039206158 1.1982871683216011E-4 62-63 0.0 0.0 0.0 2.1537713991620375 1.1982871683216011E-4 64-65 0.0 0.0 0.0 2.361704180045044 1.1982871683216011E-4 66-67 0.0 0.0 0.0 2.5860235379548473 1.1982871683216011E-4 68-69 0.0 0.0 0.0 2.8413485763449726 1.1982871683216011E-4 70-71 0.0 0.0 0.0 3.109794859228219 1.1982871683216011E-4 72-73 0.0 0.0 0.0 3.3750956382946216 2.3965743366432022E-4 74-75 2.9957179208040027E-5 0.0 0.0 3.6421638909342984 2.3965743366432022E-4 76-77 5.9914358416080055E-5 0.0 0.0 3.9265473931562225 2.3965743366432022E-4 78-79 5.9914358416080055E-5 0.0 0.0 4.234866681565371 2.3965743366432022E-4 80-81 5.9914358416080055E-5 0.0 0.0 4.572633877136022 2.3965743366432022E-4 82-83 5.9914358416080055E-5 0.0 0.0 4.912018760383907 2.995717920804003E-4 84-85 5.9914358416080055E-5 0.0 0.0 5.246610494958507 2.995717920804003E-4 86-87 5.9914358416080055E-5 0.0 0.0 5.586864136403426 2.995717920804003E-4 88-89 5.9914358416080055E-5 0.0 0.0 5.954888082974197 2.995717920804003E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6930 0.0 54.6364 1 GTATCAA 12245 0.0 42.706272 1 TATCAAC 15735 0.0 33.18717 2 ATCAACG 15720 0.0 33.03731 3 TCAACGC 15870 0.0 32.784985 4 CAACGCA 16065 0.0 32.387035 5 AACGCAG 16540 0.0 31.450321 6 ACGCAGA 18740 0.0 27.425037 7 CGCAGAG 18760 0.0 27.265808 8 GCAGAGT 19465 0.0 25.911942 9 GAGTACT 10905 0.0 25.195038 12-13 GTACATG 12505 0.0 24.946278 1 TACATGG 12280 0.0 24.941473 2 GTACACG 175 1.4492616E-7 24.493599 1 GTACTTT 11645 0.0 23.367716 14-15 TGGTATC 2915 0.0 23.330929 2 ACATGGG 12560 0.0 23.173754 3 GTGTAAG 785 0.0 22.448132 1 CAGAGTA 19190 0.0 22.429174 10-11 AGTACTT 11230 0.0 22.09549 12-13 >>END_MODULE