FastQCFastQC Report
Thu 26 May 2016
SRR938156_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938156_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1669049
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180671.0824727135033183No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149510.8957795726788129No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94940.568826918802264No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36970.22150338306424797No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30030.17992281832348841No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28470.17057617841057993No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72700.051.9468151
GTATCAA127150.041.6193281
TATCAAC161250.032.579362
ATCAACG161800.032.3160253
TCAACGC163400.031.9986274
CAACGCA165800.031.4781555
AACGCAG169050.030.9010776
ACCGTAC350.00838706727.14733578-79
ACGCAGA192700.026.9352827
CGCAGAG192200.026.8818158
GAGTACT110400.025.4222812-13
GCAGAGT201250.025.3898129
GTACTTT115550.024.1248314-15
GTGTACG1601.8591818E-623.7517831
GTAAGGT6750.023.2170074
TAAGGTG7850.022.9884685
AGTACTT112200.022.81350512-13
GTACATG122200.022.741071
TACATGG120850.022.5213872
TGGTATC33850.022.0306932
Produced by FastQC (version 0.11.5)