FastQCFastQC Report
Thu 26 May 2016
SRR938155_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938155_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2370128
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94920.40048469956053007No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77410.32660683304867927No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47840.20184563871655878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41450.053.8493771
GTATCAA73050.042.8947831
ATCAACG93350.033.14583
TCAACGC94050.032.949644
TATCAAC95600.032.8131452
CAACGCA96600.031.9814415
AACGCAG98250.031.5841396
ACGCAGA114800.026.9820527
CGCAGAG115900.026.6439868
GTGGTAT22100.025.896931
GTACATG86850.025.7550491
TACATGG86700.025.3270552
GCAGAGT124950.024.294419
TGGTATC21000.023.9910032
ACATGGG91550.023.6738193
GAGTACT74000.022.08259812-13
AGTACTT77050.020.74606912-13
CATGGGG54550.020.7369084
CAGAGTA124500.020.52859710-11
GTACTTT80200.020.04990414-15
Produced by FastQC (version 0.11.5)