FastQCFastQC Report
Thu 26 May 2016
SRR938155_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938155_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2370128
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93350.39386058474479013No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75770.3196873755341484No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49420.20851194534641168No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39900.056.502411
GTATCAA73650.043.6551481
ATCAACG92550.034.1690833
TCAACGC94050.033.6739734
TATCAAC94950.033.5114062
CAACGCA95300.033.1823925
AACGCAG98300.032.081356
TGGTATC18250.029.4494442
GTGGTAT21050.028.9214041
ACGCAGA115400.027.16297
GTACATG91650.026.7261431
CGCAGAG116500.026.7025938
TACATGG92400.026.3547882
GCAGAGT125950.024.3974349
ACATGGG95050.024.2148273
GAGTACT72750.022.78402712-13
CATGGGG52950.021.6436254
GTATAGG4550.020.906441
CAGAGTA123750.020.8397610-11
AGTACTT76150.020.73766312-13
Produced by FastQC (version 0.11.5)