##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938155_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2370128 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49431549688455 33.0 31.0 34.0 31.0 34.0 2 32.71986955978749 34.0 31.0 34.0 31.0 34.0 3 32.78930125292811 34.0 31.0 34.0 31.0 34.0 4 36.24210084856176 37.0 35.0 37.0 35.0 37.0 5 36.165585993667854 37.0 35.0 37.0 35.0 37.0 6 36.21401586749745 37.0 37.0 37.0 35.0 37.0 7 36.18734431220592 37.0 37.0 37.0 35.0 37.0 8 36.18518240365077 37.0 37.0 37.0 35.0 37.0 9 37.88114144046229 39.0 38.0 39.0 35.0 39.0 10-11 37.851646830888455 39.0 38.0 39.0 35.0 39.0 12-13 37.76656661581147 39.0 38.0 39.0 35.0 39.0 14-15 39.03149935362141 40.0 38.0 41.0 36.0 41.0 16-17 38.94519705264863 40.0 38.0 41.0 36.0 41.0 18-19 39.18080057279607 40.0 39.0 41.0 36.0 41.0 20-21 39.15150848392998 40.0 39.0 41.0 36.0 41.0 22-23 39.045343542627236 40.0 39.0 41.0 35.5 41.0 24-25 38.94389670093767 40.0 38.0 41.0 35.0 41.0 26-27 38.79385607022068 40.0 38.0 41.0 35.0 41.0 28-29 38.66967923251403 40.0 38.0 41.0 34.5 41.0 30-31 38.45387738552517 40.0 38.0 41.0 34.0 41.0 32-33 38.25589736081764 40.0 38.0 41.0 34.0 41.0 34-35 38.17620145409868 40.0 38.0 41.0 34.0 41.0 36-37 38.14322243355633 40.0 38.0 41.0 34.0 41.0 38-39 37.97914268765231 40.0 38.0 41.0 33.0 41.0 40-41 37.77540200360487 40.0 37.0 41.0 33.0 41.0 42-43 37.532180962378405 40.0 37.0 41.0 33.0 41.0 44-45 37.276300056368264 40.0 37.0 41.0 32.0 41.0 46-47 36.9175580390595 40.0 36.0 41.0 31.0 41.0 48-49 36.53531096210837 39.0 36.0 41.0 30.5 41.0 50-51 36.17801907745067 39.0 35.0 40.5 30.0 41.0 52-53 35.661610680942125 38.5 35.0 40.0 28.0 41.0 54-55 35.28716782384748 38.0 34.5 40.0 27.5 41.0 56-57 34.915900955560204 38.0 34.0 40.0 26.5 41.0 58-59 34.41809872715735 37.5 33.5 40.0 26.0 41.0 60-61 33.94932383398702 37.0 33.0 40.0 24.5 41.0 62-63 33.39792386740294 36.5 32.5 39.0 22.5 40.5 64-65 32.820736264032995 36.0 31.5 39.0 21.5 40.0 66-67 32.25550075776498 35.0 31.0 38.5 19.5 40.0 68-69 31.633623584886557 35.0 30.5 38.0 16.5 40.0 70-71 31.01544304780164 35.0 30.0 37.5 10.5 39.5 72-73 30.331053006419907 34.0 29.0 37.0 7.5 39.0 74-75 29.65972280821964 34.0 28.5 36.0 6.0 39.0 76-77 28.33836126150149 32.5 27.0 34.5 2.0 37.0 78-79 29.008152724241054 34.0 28.5 35.0 2.0 37.0 80-81 28.716965919140232 34.0 28.0 35.0 2.0 37.0 82-83 28.15906503783762 33.5 27.0 35.0 2.0 36.5 84-85 27.49712483882727 33.0 26.0 35.0 2.0 36.0 86-87 26.771623515691978 32.5 25.0 35.0 2.0 35.5 88-89 25.977708165972473 32.0 23.5 34.5 2.0 35.0 90-91 25.319766696144683 32.0 20.5 34.0 2.0 35.0 92-93 24.36347488405689 31.0 15.0 34.0 2.0 35.0 94-95 23.443236399046803 31.0 4.5 34.0 2.0 35.0 96-97 22.37453757771732 30.0 2.0 34.0 2.0 35.0 98-99 20.943005187905463 29.5 2.0 34.0 2.0 35.0 100-101 17.0591609398311 22.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21978939432286637 1101 2 0.249071005134347 1101 3 0.2618710068170884 1101 4 0.08124392906568545 1101 5 -0.02069097370898021 1101 6 0.008371293225280851 1101 7 0.14155042584326338 1101 8 0.18591786117984554 1101 9 0.040794653809804515 1101 10-11 0.2282459746467964 1101 12-13 0.3041235527198083 1101 14-15 0.4681443268416814 1101 16-17 0.43805816439556367 1101 18-19 0.33747290250107653 1101 20-21 0.276971223452982 1101 22-23 0.36101637642630635 1101 24-25 0.39854644016796925 1101 26-27 0.4608280169189314 1101 28-29 0.49315313873960065 1101 30-31 0.5227249169902635 1101 32-33 0.5139859798012836 1101 34-35 0.4567825291627585 1101 36-37 0.458463584166104 1101 38-39 0.5843436929050867 1101 40-41 0.5359797686912415 1101 42-43 0.6508657366137953 1101 44-45 0.6489258135036948 1101 46-47 0.7129658727623891 1101 48-49 0.7615721375515108 1101 50-51 0.7408578413454308 1101 52-53 0.8018718717788076 1101 54-55 0.8614845037945642 1101 56-57 0.9448055951782806 1101 58-59 1.177521473838084 1101 60-61 1.110976442034577 1101 62-63 1.2959741628248267 1101 64-65 1.3520749559640919 1101 66-67 1.5088094109590315 1101 68-69 1.7324078585872122 1101 70-71 2.0205889900085445 1101 72-73 2.071666425076895 1101 74-75 2.1607893414114763 1101 76-77 2.1586215405692606 1101 78-79 2.25918945630006 1101 80-81 2.394214079024845 1101 82-83 2.4593036214757653 1101 84-85 2.6221052922818515 1101 86-87 2.908075040316188 1101 88-89 3.315541995993943 1101 90-91 3.5954882925762597 1101 92-93 3.807904172807987 1101 94-95 4.195450422484772 1101 96-97 4.360069838700639 1101 98-99 4.577674564416498 1101 100-101 4.544779848662845 1102 1 0.14913324313807408 1102 2 0.11708243193137235 1102 3 0.14815457324721848 1102 4 0.07776383720990765 1102 5 -0.030234333818611958 1102 6 -0.0034731661511173684 1102 7 0.054102238001703995 1102 8 0.10509346028900524 1102 9 0.10349062684331756 1102 10-11 0.17006601585272563 1102 12-13 0.23538216847600069 1102 14-15 0.25036238978658787 1102 16-17 0.2924512965124535 1102 18-19 0.24498026024460273 1102 20-21 0.2282097323919814 1102 22-23 0.29222355961989166 1102 24-25 0.3044944609873639 1102 26-27 0.37632249877168533 1102 28-29 0.3639152987447005 1102 30-31 0.35523076704327394 1102 32-33 0.31484369530586065 1102 34-35 0.4206582646697967 1102 36-37 0.4070285622051486 1102 38-39 0.5422710012647869 1102 40-41 0.6004566343031925 1102 42-43 0.6703734339619274 1102 44-45 0.504676294788446 1102 46-47 0.504276806448388 1102 48-49 0.717099865583819 1102 50-51 0.7216688975713765 1102 52-53 0.6780617176008761 1102 54-55 0.8975287858408976 1102 56-57 1.0480033746602615 1102 58-59 1.1897354777807507 1102 60-61 1.0206953999702293 1102 62-63 1.2916880446552526 1102 64-65 1.1562717247587884 1102 66-67 1.2914356196242522 1102 68-69 1.3855016395028876 1102 70-71 1.3119723142244517 1102 72-73 1.7644977951334333 1102 74-75 1.861196588933197 1102 76-77 1.838831830246864 1102 78-79 1.9770127746477364 1102 80-81 2.0759555834561674 1102 82-83 2.0576185153621545 1102 84-85 2.2491220154566136 1102 86-87 2.430140892815725 1102 88-89 2.559583196791376 1102 90-91 2.651093201531001 1102 92-93 2.815742024864413 1102 94-95 3.0493186430635326 1102 96-97 3.240421765742962 1102 98-99 3.775511680805497 1102 100-101 3.5388284275201904 1103 1 0.16576109381462345 1103 2 0.21779967521878518 1103 3 0.12458146989120422 1103 4 0.08413420181237541 1103 5 0.025647645138327846 1103 6 0.059037835624486945 1103 7 0.10223917402221616 1103 8 0.11462446676146953 1103 9 0.19489783108556225 1103 10-11 0.21370014515201063 1103 12-13 0.28565391492220016 1103 14-15 0.28542434971589614 1103 16-17 0.35477415498188236 1103 18-19 0.2866505337678973 1103 20-21 0.340536981217042 1103 22-23 0.3434347800434949 1103 24-25 0.3462505742469375 1103 26-27 0.3793760739500982 1103 28-29 0.38801928307527334 1103 30-31 0.3351728474837117 1103 32-33 0.397494935809128 1103 34-35 0.5164055011273092 1103 36-37 0.536595308657553 1103 38-39 0.4982477456338472 1103 40-41 0.5266722554370418 1103 42-43 0.6683336788774952 1103 44-45 0.6360627608130969 1103 46-47 0.7779818275655828 1103 48-49 0.7228881974267836 1103 50-51 0.8279432542082503 1103 52-53 0.883669837770718 1103 54-55 0.8518144149229485 1103 56-57 1.0346352594937755 1103 58-59 1.078828053399448 1103 60-61 1.1358408636352735 1103 62-63 1.2809735059925842 1103 64-65 1.382078483669865 1103 66-67 1.521911790969071 1103 68-69 1.7055044103971504 1103 70-71 1.7187118414659857 1103 72-73 1.9488671557526906 1103 74-75 1.885408468324325 1103 76-77 1.9811657101656692 1103 78-79 2.0331050557812596 1103 80-81 2.3248187505676015 1103 82-83 2.365297537290324 1103 84-85 2.604343515110976 1103 86-87 2.7480224178264656 1103 88-89 2.8647773188507166 1103 90-91 3.173698562817922 1103 92-93 3.3647147851928274 1103 94-95 3.349485807925113 1103 96-97 3.6430417104659014 1103 98-99 3.938782483325234 1103 100-101 3.932239497760495 1104 1 0.23504176348625805 1104 2 0.18961090595534813 1104 3 0.20376433503429325 1104 4 0.13963971423478228 1104 5 -0.04364313670504316 1104 6 0.025553064257721303 1104 7 0.08728208234625612 1104 8 0.13805833424878955 1104 9 0.12757535373038564 1104 10-11 0.2675061187489334 1104 12-13 0.30612461867185203 1104 14-15 0.4910938280289585 1104 16-17 0.3913031857381526 1104 18-19 0.35881743536080535 1104 20-21 0.4167584671233584 1104 22-23 0.4320281115222713 1104 24-25 0.4281184192658074 1104 26-27 0.4066411657220286 1104 28-29 0.3974286588826317 1104 30-31 0.4500353209566086 1104 32-33 0.5006212425842662 1104 34-35 0.5697431423779875 1104 36-37 0.550058574805746 1104 38-39 0.5739122689827596 1104 40-41 0.6031441663024069 1104 42-43 0.7031147910062217 1104 44-45 0.6775952346647074 1104 46-47 0.8401314333792556 1104 48-49 0.9162538394792961 1104 50-51 0.9100857140872165 1104 52-53 1.0499855195482013 1104 54-55 1.0755483958053915 1104 56-57 1.1196871498056638 1104 58-59 1.294712213118629 1104 60-61 1.4443707580899812 1104 62-63 1.5038992071081125 1104 64-65 1.7535612076192777 1104 66-67 1.6630650187247156 1104 68-69 1.7540647444715738 1104 70-71 1.9684021809910668 1104 72-73 2.115810783567664 1104 74-75 2.034820366226292 1104 76-77 2.063187914512035 1104 78-79 2.183014631155306 1104 80-81 2.3785319301179335 1104 82-83 2.4373151356416045 1104 84-85 2.473721122836835 1104 86-87 2.7149469911374275 1104 88-89 2.951630861094909 1104 90-91 3.0048206351707094 1104 92-93 3.4084757041076834 1104 94-95 3.776104355194658 1104 96-97 3.9453546020770993 1104 98-99 4.354944846734082 1104 100-101 3.658363222759391 1105 1 0.23894292745649892 1105 2 0.20118757370719464 1105 3 0.18705959281099638 1105 4 0.13209556404629552 1105 5 0.05364156182807989 1105 6 0.034059530496918455 1105 7 0.07884544488500467 1105 8 0.12483918040372544 1105 9 0.13502212108259926 1105 10-11 0.20906584830396469 1105 12-13 0.2041361970381388 1105 14-15 0.32021563678303266 1105 16-17 0.3068755694001055 1105 18-19 0.3188398938056025 1105 20-21 0.28520548453808203 1105 22-23 0.2830976713650628 1105 24-25 0.41889054625982425 1105 26-27 0.3031255698419244 1105 28-29 0.377316932837914 1105 30-31 0.4747519718174331 1105 32-33 0.537871498544483 1105 34-35 0.5013668973733374 1105 36-37 0.523949311640898 1105 38-39 0.5174280535892848 1105 40-41 0.5962147924495085 1105 42-43 0.6294924231501042 1105 44-45 0.6241897134730934 1105 46-47 0.607154604266313 1105 48-49 0.6664411032763837 1105 50-51 0.7983942063046143 1105 52-53 0.8265811778967489 1105 54-55 0.9741125651496176 1105 56-57 0.8536924435819415 1105 58-59 1.0140968706037512 1105 60-61 1.0644667254780487 1105 62-63 1.2027222147468137 1105 64-65 1.2987563743354897 1105 66-67 1.384030260035047 1105 68-69 1.6527962197932418 1105 70-71 1.8008295331121253 1105 72-73 1.947811613963946 1105 74-75 1.8861715058980018 1105 76-77 1.8200643977837956 1105 78-79 1.9008477686849687 1105 80-81 2.129531566819562 1105 82-83 2.1888123495677263 1105 84-85 2.4843428348686167 1105 86-87 2.511812339550005 1105 88-89 2.697258211328691 1105 90-91 2.9153619497446357 1105 92-93 3.2216576788466504 1105 94-95 3.3317896916231042 1105 96-97 3.8354090024696497 1105 98-99 3.7879295079937343 1105 100-101 3.780721925642659 1106 1 0.15943330482791396 1106 2 0.11847269116489656 1106 3 0.085406281393972 1106 4 0.1171431635257818 1106 5 0.008754225344276279 1106 6 0.06141656083730851 1106 7 0.07673326078802489 1106 8 0.13578911533969062 1106 9 0.19248667830470367 1106 10-11 0.14109242182632187 1106 12-13 0.09678875508210893 1106 14-15 0.023733204072939884 1106 16-17 0.04646463864194317 1106 18-19 0.0037354489045711148 1106 20-21 0.10229883430864817 1106 22-23 0.014073631915707097 1106 24-25 -0.05638785932882939 1106 26-27 -0.1048903194807167 1106 28-29 0.05374046547267142 1106 30-31 0.12564163690885977 1106 32-33 0.25243625438312023 1106 34-35 0.13281816783426592 1106 36-37 0.23744427773911525 1106 38-39 0.13668563868619543 1106 40-41 0.2529972046130595 1106 42-43 0.22393937883806814 1106 44-45 0.4205680437244368 1106 46-47 0.5016563507220297 1106 48-49 0.4654192554577392 1106 50-51 0.5932123694441174 1106 52-53 0.7165640557818236 1106 54-55 0.7404545965173313 1106 56-57 0.7911515217272651 1106 58-59 0.9811635098750315 1106 60-61 0.9351577740363624 1106 62-63 0.8070287690924118 1106 64-65 0.8613520869579077 1106 66-67 1.1147363760544948 1106 68-69 1.054986480734268 1106 70-71 1.0451114316122236 1106 72-73 1.3499799585978742 1106 74-75 1.516891319609389 1106 76-77 1.5583699932909525 1106 78-79 1.6763878001896622 1106 80-81 1.5086864192269296 1106 82-83 1.9712628666556 1106 84-85 2.1358447867897183 1106 86-87 2.186645570150649 1106 88-89 2.2175623000897353 1106 90-91 2.4478802704076053 1106 92-93 2.63966055536196 1106 94-95 2.8762852741174534 1106 96-97 3.093057108763741 1106 98-99 3.4368371170616108 1106 100-101 3.3630875170405083 1107 1 0.24035790509471155 1107 2 0.18535542983051556 1107 3 0.22497376161868488 1107 4 0.17249141522147937 1107 5 0.1007821080265714 1107 6 0.10061296088991867 1107 7 0.15064961274112676 1107 8 0.1544680699269918 1107 9 0.19407245888828584 1107 10-11 0.19158844798042907 1107 12-13 0.22160979110037005 1107 14-15 0.38159540650421775 1107 16-17 0.2517119001642172 1107 18-19 0.2695716475405021 1107 20-21 0.24524878076037737 1107 22-23 0.26242443889765354 1107 24-25 0.3447126987005831 1107 26-27 0.3554416798428832 1107 28-29 0.315281058825164 1107 30-31 0.3895759322323116 1107 32-33 0.3758950973645341 1107 34-35 0.46011463740569525 1107 36-37 0.5588203745393727 1107 38-39 0.4854906665876655 1107 40-41 0.5037078936009038 1107 42-43 0.5343601555699351 1107 44-45 0.6496968977912942 1107 46-47 0.819789488651125 1107 48-49 0.8205918312238509 1107 50-51 0.9346141130512109 1107 52-53 1.0202518411377852 1107 54-55 1.1902216145358508 1107 56-57 1.247253676810594 1107 58-59 1.2266156640189152 1107 60-61 1.216516397188009 1107 62-63 1.2795772913478203 1107 64-65 1.5070625880570603 1107 66-67 1.654637358712371 1107 68-69 1.846634358531464 1107 70-71 1.9015160874940769 1107 72-73 2.0775399420636695 1107 74-75 1.979756528259788 1107 76-77 2.017540370759342 1107 78-79 2.1133361120097405 1107 80-81 2.2861078347803563 1107 82-83 2.4531395928829802 1107 84-85 2.479384703742916 1107 86-87 2.692120646715292 1107 88-89 2.8996100050293308 1107 90-91 3.0114129536460545 1107 92-93 3.2135708009324837 1107 94-95 3.1947519144797063 1107 96-97 3.549133429151542 1107 98-99 3.7967195926830506 1107 100-101 3.530860378397442 1108 1 0.23278553646039768 1108 2 0.2211645322325353 1108 3 0.19124900554879787 1108 4 0.12188523673919605 1108 5 0.05342887129280882 1108 6 0.08653146716240911 1108 7 0.1289730592061673 1108 8 0.13184497031274134 1108 9 0.16393858096679992 1108 10-11 0.18583501999248853 1108 12-13 0.3216843478121021 1108 14-15 0.3123703100667399 1108 16-17 0.32588124114771233 1108 18-19 0.3098351082685582 1108 20-21 0.26746384445773685 1108 22-23 0.3079340178143113 1108 24-25 0.2974020079164532 1108 26-27 0.3725046316403393 1108 28-29 0.343734574336203 1108 30-31 0.32998445438862944 1108 32-33 0.43930922748220524 1108 34-35 0.46841433917884956 1108 36-37 0.37309352763995207 1108 38-39 0.3448993994477192 1108 40-41 0.48907370772777625 1108 42-43 0.44856928714632005 1108 44-45 0.6561890045046326 1108 46-47 0.7046625801613828 1108 48-49 0.6545868553737435 1108 50-51 0.7180852142214249 1108 52-53 0.6912444178825723 1108 54-55 0.7899748480280238 1108 56-57 0.8607593820198431 1108 58-59 1.036245635817032 1108 60-61 0.9195290556210267 1108 62-63 1.1044772070663242 1108 64-65 1.0818879470638265 1108 66-67 1.193407254008818 1108 68-69 1.4489075278400314 1108 70-71 1.4698614957731202 1108 72-73 1.506854506555225 1108 74-75 1.4122373218025253 1108 76-77 1.4810829491249322 1108 78-79 1.5759325494955583 1108 80-81 1.78654828665886 1108 82-83 2.057194424482063 1108 84-85 2.231504302433308 1108 86-87 2.1920817475613497 1108 88-89 2.303354453795901 1108 90-91 2.3752387664762935 1108 92-93 2.771605097019876 1108 94-95 2.898456653693291 1108 96-97 3.2258952366655613 1108 98-99 3.285372790517666 1108 100-101 3.023618652571642 1109 1 0.04767678556217447 1109 2 0.026330857135704377 1109 3 0.037019463161364285 1109 4 0.037438981240306646 1109 5 -0.033251991703849626 1109 6 0.0012833819143196479 1109 7 0.022369136812770307 1109 8 0.02667382566791332 1109 9 0.041683642922386355 1109 10-11 0.026182172839646967 1109 12-13 0.04474637350570276 1109 14-15 0.07345790668064467 1109 16-17 0.12450586214075798 1109 18-19 0.07444878406644762 1109 20-21 0.13436996465333095 1109 22-23 0.07184773567539793 1109 24-25 0.13967924822000555 1109 26-27 0.0943726758768122 1109 28-29 0.15331863063079254 1109 30-31 0.1664407612396701 1109 32-33 0.2128635240628185 1109 34-35 0.2674606875672936 1109 36-37 0.130473209361746 1109 38-39 0.09621387444641272 1109 40-41 0.27512537535839243 1109 42-43 0.4223663234433559 1109 44-45 0.2982618439459017 1109 46-47 0.36202495462902107 1109 48-49 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0.6634156570843501 2316 62-63 0.6748629700671742 2316 64-65 0.8381196203351422 2316 66-67 1.0435104223664595 2316 68-69 1.1392549042487907 2316 70-71 1.2922672975587126 2316 72-73 1.3993017864705344 2316 74-75 1.7528650444634977 2316 76-77 1.7983543392863446 2316 78-79 1.8848749391604507 2316 80-81 1.8845283649778146 2316 82-83 2.1678580042952547 2316 84-85 2.2307109248816204 2316 86-87 2.5102009684938658 2316 88-89 2.874803518371568 2316 90-91 3.1408305383020796 2316 92-93 3.1730213491394537 2316 94-95 3.631647003411775 2316 96-97 4.213677807888033 2316 98-99 4.568322843448534 2316 100-101 4.709622652833957 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 3.0 4 12.0 5 45.0 6 84.0 7 236.0 8 501.0 9 981.0 10 1691.0 11 2762.0 12 4164.0 13 6717.0 14 8810.0 15 12310.0 16 16356.0 17 22740.0 18 23893.0 19 19505.0 20 19484.0 21 21289.0 22 23407.0 23 26157.0 24 29546.0 25 33647.0 26 38365.0 27 44279.0 28 53512.0 29 64267.0 30 77233.0 31 92860.0 32 112759.0 33 140888.0 34 182772.0 35 249005.0 36 346782.0 37 443262.0 38 240312.0 39 9487.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.03716930056098 19.001674171184003 14.478711698271148 23.482444829983866 2 15.794670673767708 19.82725917411352 39.750602689770794 24.627467462347976 3 19.452536142605915 23.57471722187155 29.487995220366102 27.484751415156435 4 13.22491223924931 16.498810166745425 36.07928846283669 34.19698913116857 5 14.258569990491049 36.82156838144626 33.87227251279231 15.04758911527038 6 32.99679721803937 35.43173002965664 17.01828636187006 14.55318639043393 7 29.484188195743016 32.24661284116301 19.827199206118827 18.44199975697515 8 26.59240029078733 35.299565380105705 19.140616264935446 18.967418064171515 9 26.407940836950576 16.758208839353824 20.00685195061195 36.82699837308365 10-11 25.54741769220903 26.420134271229234 26.620756347336517 21.411691689225222 12-13 25.862548351818976 23.602501637042387 27.5612751716363 22.97367483950234 14-15 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28.612059026125884 22.610415543673067 40-41 22.66597014654561 26.21961524155373 28.57024406124117 22.544170550659494 42-43 22.57318222449321 26.18920785158934 28.444996532687988 22.792613391229462 44-45 22.54364844204402 26.42631815562957 28.353876899779728 22.676156502546682 46-47 22.55741040146355 26.74153041523496 27.88805077194143 22.813008411360062 48-49 22.738244516751838 26.57369137869347 27.92960970884273 22.75845439571196 50-51 22.645949923379664 26.76055892339992 27.783668223825885 22.809822929394528 52-53 22.85931392734907 26.831377883388573 27.543301458824164 22.76600673043819 54-55 22.65983103022284 26.66229418833076 27.731223798883438 22.946650982562964 56-57 22.596986323101536 27.00149527789216 27.708946521031773 22.692571877974522 58-59 22.813831151735265 26.576243983447306 27.693377741624083 22.916547123193347 60-61 22.71227545516529 26.67480406121526 27.85750389852362 22.755416585095826 62-63 22.56705123098837 26.76935591664248 27.736961885602803 22.92663096676635 64-65 22.498848163474715 26.527195999540954 27.75759368270406 23.216362154280276 66-67 22.43500978428995 26.794398272496906 27.64426870132643 23.126323241886716 68-69 22.374568611885394 26.624454851366636 27.766237059411363 23.234739477336603 70-71 22.367230799349233 26.752099464670266 27.649139624526608 23.231530111453896 72-73 22.654904146486665 26.673127642527174 27.39355317246713 23.278415038519032 74-75 22.470073346249652 26.84302282408376 27.287154955344185 23.3997488743224 76-77 22.425919612780408 26.751550971086797 27.521087468693672 23.301441947439123 78-79 22.480747875220242 26.81817184557121 27.472566882463735 23.22851339674482 80-81 22.22569601784884 27.062189942644398 27.471957187086254 23.240156852420505 82-83 22.453559204037827 26.966135216526816 27.289224553945797 23.291081025489564 84-85 22.483894732223025 26.66567371242245 27.32967318584728 23.52075836950725 86-87 22.43631179268728 26.558208181895665 27.573920007155934 23.431560018261123 88-89 22.39790424820938 26.751171244759774 27.28502426873148 23.565900238299367 90-91 22.445365652985664 26.73522989778804 27.509488096368255 23.309916352858032 92-93 22.43937880148245 26.414143033625187 27.38442818278169 23.76204998211067 94-95 22.27704579668271 26.52449572343772 27.379744891415147 23.818713588464423 96-97 22.44903168588823 26.49166742084646 27.25063598979716 23.808664903468152 98-99 22.325714054262047 26.39908055598685 27.057378335684824 24.21782705406628 100-101 22.582282475883158 25.99515722357611 27.201463380880696 24.221096919660035 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 764.0 1 520.0 2 417.5 3 609.0 4 509.0 5 439.5 6 955.0 7 2083.5 8 3259.0 9 3748.5 10 3190.0 11 2532.0 12 2536.0 13 2652.0 14 2759.5 15 2871.5 16 2952.5 17 3053.0 18 3165.5 19 3318.0 20 3611.0 21 4194.5 22 5001.0 23 5988.5 24 7161.5 25 8852.0 26 11321.5 27 14101.5 28 16446.5 29 19359.0 30 23340.0 31 26819.5 32 30639.5 33 34644.5 34 38559.5 35 42391.0 36 46896.5 37 51766.0 38 55848.5 39 59035.5 40 62268.0 41 68438.5 42 74556.5 43 80263.0 44 86317.5 45 90275.5 46 91614.0 47 93373.5 48 96825.0 49 97785.5 50 96298.0 51 92836.5 52 88874.0 53 86831.0 54 85518.5 55 79927.5 56 71164.0 57 65046.5 58 60112.5 59 52968.0 60 44080.0 61 35796.0 62 28521.0 63 22214.5 64 17571.5 65 13649.5 66 9951.0 67 7922.0 68 5964.0 69 3858.5 70 2800.0 71 2142.0 72 1712.5 73 1372.0 74 1100.0 75 930.5 76 610.5 77 305.0 78 170.5 79 98.5 80 58.0 81 34.0 82 13.0 83 6.5 84 5.0 85 6.0 86 4.5 87 3.0 88 2.0 89 0.5 90 0.0 91 0.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.014134257727852675 3 0.0030378106161355 4 0.0020252070774236667 5 0.12172338371598496 6 4.219181411299305E-5 7 0.0 8 4.641099552429236E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.1095907056496527E-5 18-19 0.0 20-21 0.0 22-23 2.5315088467795833E-4 24-25 5.906853975819027E-4 26-27 0.006096717139327496 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010948775762321698 36-37 7.172608399208819E-4 38-39 0.0067717861651353855 40-41 0.010611241249417752 42-43 0.005759182626423552 44-45 0.0035230164784349196 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 8.43836282259861E-5 68-69 7.383567469773784E-4 70-71 0.0 72-73 4.219181411299305E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 8.43836282259861E-5 82-83 0.0017087684715762184 84-85 0.004050414154847333 86-87 0.0027846597314575418 88-89 0.0 90-91 0.002236166147988632 92-93 0.0 94-95 0.0 96-97 1.4767134939547568E-4 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2370128.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.491894653289215 #Duplication Level Percentage of deduplicated Percentage of total 1 75.516744854206 38.88500270592096 2 13.495484073815698 13.898160884481205 3 4.501373768571008 6.953527916590134 4 2.0381742932871 4.197978239799262 5 1.0977923066067108 2.8263702901492063 6 0.6977976742193409 2.1558554598127517 7 0.48480106441515625 1.7474327735667396 8 0.3153222014627582 1.298923006365087 9 0.23174548166407483 1.0739712535400079 >10 1.4190785601695033 13.541725994067665 >50 0.12601425327047516 4.508486615222345 >100 0.07213816652298805 6.642463963466752 >500 0.002547264080527881 0.8251027452845863 >1k 8.216980904926337E-4 0.7294380456747346 >5k 1.6433961809852673E-4 0.7155601060586492 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9335 0.39386058474479013 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7577 0.3196873755341484 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4942 0.20851194534641168 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 4.219181411299305E-5 0.0 0.0 0.0 0.0 7 4.219181411299305E-5 0.0 0.0 0.0 0.0 8 8.43836282259861E-5 0.0 0.0 0.0 0.0 9 8.43836282259861E-5 0.0 0.0 0.0 0.0 10-11 8.43836282259861E-5 0.0 0.0 2.1095907056496527E-5 0.0 12-13 1.2657544233897917E-4 0.0 0.0 8.43836282259861E-5 0.0 14-15 1.2657544233897917E-4 0.0 0.0 8.43836282259861E-5 0.0 16-17 1.2657544233897917E-4 0.0 0.0 8.43836282259861E-5 0.0 18-19 1.2657544233897917E-4 0.0 0.0 1.0547953528248263E-4 0.0 20-21 1.2657544233897917E-4 0.0 0.0 2.742467917344548E-4 0.0 22-23 1.2657544233897917E-4 0.0 0.0 4.430140481864271E-4 0.0 24-25 1.2657544233897917E-4 0.0 0.0 6.539731187513924E-4 0.0 26-27 1.2657544233897917E-4 0.0 0.0 0.001202466702220302 0.0 28-29 1.476713493954757E-4 0.0 0.0 0.004029318247790837 0.0 30-31 1.687672564519722E-4 0.0 0.0 0.013142750096197336 0.0 32-33 1.687672564519722E-4 0.0 0.0 0.03147509332829282 0.0 34-35 1.687672564519722E-4 0.0 0.0 0.06027100646041058 0.0 36-37 1.687672564519722E-4 0.0 0.0 0.11094337521011524 0.0 38-39 1.687672564519722E-4 0.0 0.0 0.18212096561873453 0.0 40-41 1.687672564519722E-4 0.0 0.0 0.2607454112182971 0.0 42-43 1.687672564519722E-4 0.0 0.0 0.3386104041638257 0.0 44-45 1.687672564519722E-4 0.0 0.0 0.4294704758561563 0.0 46-47 1.8986316350846872E-4 0.0 0.0 0.5395067270628422 0.0 48-49 2.5315088467795833E-4 0.0 0.0 0.6540575023796183 0.0 50-51 2.5315088467795833E-4 0.0 0.0 0.7736712953899536 0.0 52-53 2.742467917344548E-4 0.0 0.0 0.8904793327617748 0.0 54-55 2.9534269879095135E-4 0.0 0.0 1.0161898429114378 0.0 56-57 3.375345129039444E-4 0.0 0.0 1.1612452998319078 0.0 58-59 3.375345129039444E-4 0.0 0.0 1.315519668136067 4.219181411299305E-5 60-61 3.375345129039444E-4 0.0 0.0 1.472641983892853 4.219181411299305E-5 62-63 3.375345129039444E-4 0.0 0.0 1.6325067675669838 4.219181411299305E-5 64-65 3.375345129039444E-4 0.0 0.0 1.797497856655843 4.219181411299305E-5 66-67 3.375345129039444E-4 0.0 0.0 1.979344575482843 4.219181411299305E-5 68-69 3.797263270169375E-4 0.0 0.0 2.18180199550404 4.219181411299305E-5 70-71 3.797263270169375E-4 0.0 0.0 2.3956090135216326 4.219181411299305E-5 72-73 4.219181411299305E-4 0.0 0.0 2.6060406864101857 4.219181411299305E-5 74-75 4.641099552429236E-4 0.0 0.0 2.8201430471265687 6.328772116948958E-5 76-77 4.641099552429236E-4 0.0 0.0 3.0538646014054938 8.43836282259861E-5 78-79 4.641099552429236E-4 0.0 0.0 3.3134286418286267 8.43836282259861E-5 80-81 7.59452654033875E-4 0.0 0.0 3.5884770780312287 8.43836282259861E-5 82-83 7.59452654033875E-4 0.0 0.0 3.86768140792396 8.43836282259861E-5 84-85 7.805485610903715E-4 0.0 0.0 4.140957787933816 8.43836282259861E-5 86-87 8.016444681468681E-4 0.0 0.0 4.421343488621711 8.43836282259861E-5 88-89 8.016444681468681E-4 0.0 0.0 4.729449211181843 8.43836282259861E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3990 0.0 56.50241 1 GTATCAA 7365 0.0 43.655148 1 ATCAACG 9255 0.0 34.169083 3 TCAACGC 9405 0.0 33.673973 4 TATCAAC 9495 0.0 33.511406 2 CAACGCA 9530 0.0 33.182392 5 AACGCAG 9830 0.0 32.08135 6 TGGTATC 1825 0.0 29.449444 2 GTGGTAT 2105 0.0 28.921404 1 ACGCAGA 11540 0.0 27.1629 7 GTACATG 9165 0.0 26.726143 1 CGCAGAG 11650 0.0 26.702593 8 TACATGG 9240 0.0 26.354788 2 GCAGAGT 12595 0.0 24.397434 9 ACATGGG 9505 0.0 24.214827 3 GAGTACT 7275 0.0 22.784027 12-13 CATGGGG 5295 0.0 21.643625 4 GTATAGG 455 0.0 20.90644 1 CAGAGTA 12375 0.0 20.83976 10-11 AGTACTT 7615 0.0 20.737663 12-13 >>END_MODULE