FastQCFastQC Report
Thu 26 May 2016
SRR938154_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938154_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2330488
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93720.40214753304887213No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74980.3217351902262531No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46350.1988853836621343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41550.054.484261
GTATCAA76100.043.4922941
ATCAACG93650.034.613493
TCAACGC94350.034.2559364
TATCAAC95400.034.1280142
CAACGCA95350.033.9465185
AACGCAG99350.032.7655036
ACGCAGA116000.027.9697787
CGCAGAG116750.027.7087218
GTACATG85050.026.6175291
TGGTATC20400.026.0949612
TACATGG85650.025.6379572
GCAGAGT126200.025.1429029
GTGGTAT22800.025.1368081
ACATGGG87600.023.7107943
GAGTACT76400.023.47249212-13
GTACTTT81800.021.66418814-15
CAGAGTA124350.021.44945710-11
CATGGGG51700.021.4207254
AGTACTT79100.020.32908412-13
Produced by FastQC (version 0.11.5)