##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938154_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2330488 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.8851090415398 33.0 31.0 34.0 30.0 34.0 2 32.07817804682968 34.0 31.0 34.0 30.0 34.0 3 32.16880842124053 34.0 31.0 34.0 30.0 34.0 4 35.67369323506493 37.0 35.0 37.0 35.0 37.0 5 35.68116205704556 37.0 35.0 37.0 35.0 37.0 6 35.70363374537865 37.0 35.0 37.0 35.0 37.0 7 35.644704456748975 37.0 35.0 37.0 33.0 37.0 8 35.61997315583689 37.0 35.0 37.0 33.0 37.0 9 37.261285190054615 39.0 37.0 39.0 34.0 39.0 10-11 37.171729697814364 39.0 37.0 39.0 34.0 39.0 12-13 37.06956418569845 39.0 37.0 39.0 33.0 39.0 14-15 38.28004370758399 40.0 38.0 41.0 33.5 41.0 16-17 38.13778594869401 40.0 38.0 41.0 33.0 41.0 18-19 38.263649072640575 40.0 38.0 41.0 33.5 41.0 20-21 38.24422717473765 40.0 38.0 41.0 34.0 41.0 22-23 38.15462233660933 40.0 38.0 41.0 33.5 41.0 24-25 38.05354243403099 40.0 38.0 41.0 33.0 41.0 26-27 37.8949385278963 40.0 38.0 41.0 33.0 41.0 28-29 37.69375255311334 40.0 38.0 41.0 32.5 41.0 30-31 37.49772172180247 40.0 37.0 41.0 32.0 41.0 32-33 37.27335712520296 40.0 37.0 41.0 31.5 41.0 34-35 37.05573424965071 40.0 36.5 41.0 31.0 41.0 36-37 36.81621038168829 39.5 36.0 41.0 30.0 41.0 38-39 36.55888123002564 39.0 36.0 41.0 30.0 41.0 40-41 36.36667642141903 39.0 35.0 41.0 30.0 41.0 42-43 36.218592414979184 39.0 35.0 41.0 30.0 41.0 44-45 35.97179131581025 39.0 35.0 41.0 28.5 41.0 46-47 35.60519942604296 39.0 35.0 40.0 27.0 41.0 48-49 35.24790816344044 39.0 35.0 40.0 26.0 41.0 50-51 34.57234858106972 38.0 33.5 39.5 24.5 40.5 52-53 34.7089931379179 38.0 34.0 40.0 26.0 40.5 54-55 34.827509731867316 38.0 34.0 40.0 25.5 41.0 56-57 34.54708284273508 38.0 34.0 40.0 24.0 41.0 58-59 34.2467133493071 38.0 34.0 40.0 23.0 41.0 60-61 33.840470322095626 37.5 33.5 40.0 22.5 41.0 62-63 33.30357118337447 37.0 33.0 40.0 20.5 41.0 64-65 32.7837661039233 36.0 32.0 39.0 18.5 41.0 66-67 32.26237959603311 36.0 31.5 39.0 15.0 40.0 68-69 31.674267792839956 35.0 31.0 38.5 10.0 40.0 70-71 31.135578471118496 35.0 30.5 38.0 7.5 40.0 72-73 30.493974223424452 35.0 29.5 37.0 2.0 39.0 74-75 29.802427860602585 34.0 29.0 36.5 2.0 39.0 76-77 29.1334407214283 34.0 28.5 36.0 2.0 38.5 78-79 28.40755712966555 34.0 26.5 35.5 2.0 37.5 80-81 27.74438079063269 33.0 26.0 35.0 2.0 37.0 82-83 27.025349197249675 33.0 25.5 35.0 2.0 36.0 84-85 26.368220089526314 32.0 24.0 35.0 2.0 36.0 86-87 25.682015311814524 32.0 21.5 34.5 2.0 35.5 88-89 24.89526206528418 31.0 19.0 34.0 2.0 35.0 90-91 24.07990236379677 31.0 11.0 34.0 2.0 35.0 92-93 23.186568864546825 30.5 3.5 34.0 2.0 35.0 94-95 22.233736668028328 30.0 2.0 34.0 2.0 35.0 96-97 21.03010592631243 29.0 2.0 34.0 2.0 35.0 98-99 19.541894444425374 28.0 2.0 34.0 2.0 35.0 100-101 15.80763814274092 13.0 2.0 30.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.45683653661415846 1101 2 0.33342347135481987 1101 3 0.2954621317676498 1101 4 0.28861957300568974 1101 5 0.3008883813167387 1101 6 0.38754475448697434 1101 7 0.19576310383113338 1101 8 0.17561520517808304 1101 9 0.33538475515183563 1101 10-11 0.3998842515289738 1101 12-13 0.4256039589965752 1101 14-15 0.47810383908070264 1101 16-17 0.5685973606844357 1101 18-19 0.5811360009574997 1101 20-21 0.5860501650643499 1101 22-23 0.6650509984908339 1101 24-25 0.6058834053055122 1101 26-27 0.6825408424633466 1101 28-29 0.7064437417747911 1101 30-31 0.8000828922509626 1101 32-33 0.6095090013208662 1101 34-35 0.6047428816693525 1101 36-37 0.80601433758806 1101 38-39 0.8630732670910248 1101 40-41 0.7902844928814545 1101 42-43 0.9743955251827359 1101 44-45 0.957997326229048 1101 46-47 1.0065035728177847 1101 48-49 1.1013241002838114 1101 50-51 0.9570538365818493 1101 52-53 1.0138447835198576 1101 54-55 1.228872075715806 1101 56-57 1.218913782375438 1101 58-59 1.080746221941851 1101 60-61 1.1802002925212491 1101 62-63 1.2799161561545844 1101 64-65 1.2570816864037013 1101 66-67 1.293316598695725 1101 68-69 1.4698522243152432 1101 70-71 1.7601894265542803 1101 72-73 1.9316531737736966 1101 74-75 1.9599124117336295 1101 76-77 1.962167048027279 1101 78-79 2.0376978103128813 1101 80-81 2.372939377985265 1101 82-83 2.4021214817307843 1101 84-85 2.397843570829288 1101 86-87 2.8167844088223006 1101 88-89 3.2145309715504027 1101 90-91 3.180771272443163 1101 92-93 3.355230725088248 1101 94-95 3.632273534190535 1101 96-97 4.06992683435578 1101 98-99 4.155865717748494 1101 100-101 3.9475406280820966 1102 1 0.43664588634356605 1102 2 0.368254747858316 1102 3 0.2944762200555573 1102 4 0.2797972087536138 1102 5 0.3787760166660661 1102 6 0.33443892922925045 1102 7 0.10655965053707916 1102 8 0.2886013381508903 1102 9 0.3981111508229347 1102 10-11 0.3421635744711935 1102 12-13 0.468335646215273 1102 14-15 0.5687689756319187 1102 16-17 0.5850551515426261 1102 18-19 0.5190909784694426 1102 20-21 0.5247369501868491 1102 22-23 0.5898720782919256 1102 24-25 0.6207666455385166 1102 26-27 0.5856223453116698 1102 28-29 0.624091032673249 1102 30-31 0.5851136694536265 1102 32-33 0.6665212577002038 1102 34-35 0.6749914298492783 1102 36-37 0.8163901025440978 1102 38-39 0.7187732522047341 1102 40-41 0.9491419578681644 1102 42-43 1.0719345894939565 1102 44-45 0.9143803927886651 1102 46-47 1.0627498563292264 1102 48-49 1.142238216147966 1102 50-51 1.2076419670833758 1102 52-53 1.1114600078839203 1102 54-55 1.2985630997705826 1102 56-57 1.3756722193637216 1102 58-59 1.3602178236985978 1102 60-61 1.2066101381349554 1102 62-63 1.40520645915975 1102 64-65 1.5136397888978408 1102 66-67 1.6231162682688733 1102 68-69 1.7324923527115423 1102 70-71 1.8313527088268238 1102 72-73 2.162318180171546 1102 74-75 2.0483462315964935 1102 76-77 2.2325248640213395 1102 78-79 2.352764322747813 1102 80-81 2.2745069328672756 1102 82-83 2.6235703082762107 1102 84-85 2.6666094485225464 1102 86-87 2.7807914118866037 1102 88-89 3.052226476134649 1102 90-91 3.3226334538623057 1102 92-93 3.641885775567413 1102 94-95 4.002649225935315 1102 96-97 3.938514849346195 1102 98-99 3.839419884730379 1102 100-101 4.032918386011486 1103 1 0.5416934300480953 1103 2 0.39037614330094783 1103 3 0.3683186510667724 1103 4 0.2761806297286853 1103 5 0.26859638988026546 1103 6 0.2888303232758105 1103 7 0.3118685682779372 1103 8 0.44973339020852876 1103 9 0.3540343363286951 1103 10-11 0.5480956792689895 1103 12-13 0.603948828153321 1103 14-15 0.7914038120598477 1103 16-17 0.7473885934586448 1103 18-19 0.7518053904941979 1103 20-21 0.7277303421548282 1103 22-23 0.6653087358066259 1103 24-25 0.7726373700952749 1103 26-27 0.8553890476555068 1103 28-29 0.8200056091232142 1103 30-31 0.7738679933712902 1103 32-33 0.9818469281982871 1103 34-35 0.9618374130655027 1103 36-37 1.099152601186816 1103 38-39 1.1915558386443124 1103 40-41 1.1456320225524195 1103 42-43 1.2302237141623849 1103 44-45 1.1492893384507923 1103 46-47 1.2552626923538597 1103 48-49 1.430457770338478 1103 50-51 1.3001520885408695 1103 52-53 1.4881728997510777 1103 54-55 1.5017182023812197 1103 56-57 1.4576164699105192 1103 58-59 1.6202574563788872 1103 60-61 1.6247306494042917 1103 62-63 1.67304038969786 1103 64-65 1.7095566921196408 1103 66-67 1.797168108745396 1103 68-69 1.9789572107008944 1103 70-71 1.9999490449367734 1103 72-73 2.275350041433896 1103 74-75 2.362748561470699 1103 76-77 2.408532744021958 1103 78-79 2.744676463843259 1103 80-81 2.979801114386504 1103 82-83 3.040339352903697 1103 84-85 2.890753196486621 1103 86-87 3.081458807608442 1103 88-89 3.450396470085046 1103 90-91 3.8260301529486647 1103 92-93 3.8088863339926533 1103 94-95 4.161457608518653 1103 96-97 4.420465692413192 1103 98-99 4.868519268911431 1103 100-101 4.670862217739346 1104 1 0.19806247938763377 1104 2 0.27001599646740715 1104 3 0.20949274130580875 1104 4 0.23419192165187752 1104 5 0.23855862167300046 1104 6 0.23344739026620687 1104 7 0.14710524583186668 1104 8 0.17029104935046036 1104 9 0.14437261228711407 1104 10-11 0.2044269122275324 1104 12-13 0.1998629729250183 1104 14-15 0.3893838874035751 1104 16-17 0.5157095461424035 1104 18-19 0.37593754068063134 1104 20-21 0.4060008569393503 1104 22-23 0.33906392652644257 1104 24-25 0.39746563073444463 1104 26-27 0.5211726897824747 1104 28-29 0.4684763339452189 1104 30-31 0.45204794153635675 1104 32-33 0.4096292021912191 1104 34-35 0.5705578223495422 1104 36-37 0.7151954180013789 1104 38-39 0.6907790371875322 1104 40-41 0.7358337259756453 1104 42-43 0.7406000955442025 1104 44-45 0.7560001804313075 1104 46-47 0.649979719841788 1104 48-49 0.8232307468007889 1104 50-51 0.9340023260373727 1104 52-53 0.7870774290455245 1104 54-55 1.012139144466758 1104 56-57 1.223999393285375 1104 58-59 1.0289545014020547 1104 60-61 1.1160336043811157 1104 62-63 1.3140752578463406 1104 64-65 1.36704843032431 1104 66-67 1.3901188462852616 1104 68-69 1.5435673406736292 1104 70-71 1.803742726469853 1104 72-73 1.8883393661345025 1104 74-75 1.9900647545895573 1104 76-77 2.084450212781295 1104 78-79 2.3952897154739397 1104 80-81 2.4541187334478884 1104 82-83 2.605956313911875 1104 84-85 2.6451261671680903 1104 86-87 2.8397531202174378 1104 88-89 2.9701431244192342 1104 90-91 3.193480847132168 1104 92-93 3.4771444199538664 1104 94-95 3.81512266618261 1104 96-97 3.8841111433675124 1104 98-99 3.955363503960715 1104 100-101 4.228851590613681 1105 1 0.25907801018327703 1105 2 0.20035437677557155 1105 3 0.2358664801422634 1105 4 0.15776882234334266 1105 5 0.24257011730227163 1105 6 0.23522548117858832 1105 7 0.17180288683294265 1105 8 0.26807073316496144 1105 9 0.289756259420308 1105 10-11 0.2782117674043221 1105 12-13 0.25735939446484224 1105 14-15 0.38234445054460053 1105 16-17 0.42656289750263454 1105 18-19 0.41483380153784566 1105 20-21 0.3720982537140003 1105 22-23 0.305395420039261 1105 24-25 0.3724473903343224 1105 26-27 0.4209444759214378 1105 28-29 0.5773198299197944 1105 30-31 0.44038000314713344 1105 32-33 0.5731980757142381 1105 34-35 0.6071346979778625 1105 36-37 0.6660378877794031 1105 38-39 0.5334836081482663 1105 40-41 0.7034326488173548 1105 42-43 0.6001524956188362 1105 44-45 0.7105363312334632 1105 46-47 0.83391431338719 1105 48-49 0.8838712006664977 1105 50-51 0.8935629412540465 1105 52-53 0.8555932800924637 1105 54-55 0.9984823410759915 1105 56-57 0.9294961239373265 1105 58-59 0.8099637037535317 1105 60-61 0.8894263953851222 1105 62-63 1.0691787407880895 1105 64-65 1.0837170579500182 1105 66-67 1.2143130661670227 1105 68-69 1.4277352303289597 1105 70-71 1.576763399387449 1105 72-73 1.7257519584156142 1105 74-75 1.7303111879647552 1105 76-77 1.862614922201974 1105 78-79 1.9627393385520797 1105 80-81 2.0723012098537197 1105 82-83 2.2251743855534833 1105 84-85 2.320787660151801 1105 86-87 2.601160854425565 1105 88-89 2.7152721717690866 1105 90-91 2.9224840231896216 1105 92-93 3.1049626313921266 1105 94-95 3.3365793292197665 1105 96-97 3.6053861682217985 1105 98-99 4.141407056745091 1105 100-101 4.098411669757523 1106 1 0.14761485458172885 1106 2 0.12288038298438408 1106 3 0.13250745830249855 1106 4 0.20379981194085417 1106 5 0.1451462297144488 1106 6 -0.05173873645661331 1106 7 0.017587430158471307 1106 8 0.10182025661139704 1106 9 0.04482951251109313 1106 10-11 0.15752661953656144 1106 12-13 0.23681108554920627 1106 14-15 0.36471264944443504 1106 16-17 0.1124324035208275 1106 18-19 0.1893831180238763 1106 20-21 0.2107433438507087 1106 22-23 0.09739136252605363 1106 24-25 0.16871801829576327 1106 26-27 0.06893954699599902 1106 28-29 0.16782688291255 1106 30-31 0.1619733259509033 1106 32-33 0.1556833706328078 1106 34-35 0.2876994820310159 1106 36-37 0.23460577623863088 1106 38-39 5.510336697440721E-4 1106 40-41 0.40337886634364395 1106 42-43 0.26066935199921204 1106 44-45 0.4957774673371702 1106 46-47 0.6163989275149717 1106 48-49 0.662839530278184 1106 50-51 0.6707121978304826 1106 52-53 0.6921864667380788 1106 54-55 0.1924144526167595 1106 56-57 0.5645200877737153 1106 58-59 0.8758656154141207 1106 60-61 0.8590815067625002 1106 62-63 0.5502827111600723 1106 64-65 0.8513788138456704 1106 66-67 1.2508048149824447 1106 68-69 1.1058273821725386 1106 70-71 1.0074391523420836 1106 72-73 1.2193273355737162 1106 74-75 1.3839493340352327 1106 76-77 1.7789232659740968 1106 78-79 2.02300225996159 1106 80-81 2.100290562285508 1106 82-83 2.1876471539212226 1106 84-85 2.2679766749113455 1106 86-87 1.9547935541695907 1106 88-89 2.3897336662410638 1106 90-91 2.51167646913483 1106 92-93 2.7513891604482765 1106 94-95 2.755585646958451 1106 96-97 2.6732473958237826 1106 98-99 2.8306730280974115 1106 100-101 2.9874699999138326 1107 1 0.25824678908568544 1107 2 0.11353722178975545 1107 3 -0.01491272810733335 1107 4 0.04731125366997446 1107 5 0.012931916703976754 1107 6 0.0842759923812082 1107 7 0.07894009967936455 1107 8 0.14316835980393705 1107 9 0.14604558080522878 1107 10-11 0.1789917632301723 1107 12-13 0.1477626630375397 1107 14-15 0.33405380275000596 1107 16-17 0.31098550894795807 1107 18-19 0.25602479402831335 1107 20-21 0.37045197323972445 1107 22-23 0.3493420105560503 1107 24-25 0.40002716196346455 1107 26-27 0.28011304480351384 1107 28-29 0.4031912338138355 1107 30-31 0.5292725856227776 1107 32-33 0.5403304679377001 1107 34-35 0.539833026084743 1107 36-37 0.4756684029461482 1107 38-39 0.5376726113523844 1107 40-41 0.556607762458043 1107 42-43 0.6826337152069115 1107 44-45 0.6506670820167741 1107 46-47 0.4814261959438255 1107 48-49 0.7029085757536322 1107 50-51 0.507889478241907 1107 52-53 0.5053345777030955 1107 54-55 0.6394889258697347 1107 56-57 0.7514093008755154 1107 58-59 0.7175284228316485 1107 60-61 0.8586133771435414 1107 62-63 0.8789244389810023 1107 64-65 0.984276942622607 1107 66-67 1.0776687513517942 1107 68-69 1.2302714883704375 1107 70-71 1.3740508719371398 1107 72-73 1.490041544951204 1107 74-75 1.5000400549969477 1107 76-77 1.7455164449806446 1107 78-79 1.8537360786427328 1107 80-81 1.976596256917965 1107 82-83 2.293327132028754 1107 84-85 2.375927267886322 1107 86-87 2.4713239056797143 1107 88-89 2.6784646317766203 1107 90-91 2.9417073647181233 1107 92-93 3.1720190060999016 1107 94-95 3.589606493380842 1107 96-97 3.841465183777739 1107 98-99 3.991182384212731 1107 100-101 3.9234584156893657 1108 1 0.21353741496021783 1108 2 0.11579012366530606 1108 3 0.1148021587310879 1108 4 0.16096478923930846 1108 5 0.11578301766800081 1108 6 0.1629218849426124 1108 7 0.15914080105216044 1108 8 0.17062724894246628 1108 9 0.2010778432796414 1108 10-11 0.1958295993531678 1108 12-13 0.25522064181979687 1108 14-15 0.35700175397330014 1108 16-17 0.36210730082335374 1108 18-19 0.3047079345673893 1108 20-21 0.30350078703324357 1108 22-23 0.11945812542523981 1108 24-25 0.19696077860048433 1108 26-27 0.3145799913613132 1108 28-29 0.40211528159497334 1108 30-31 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-0.7676839506363109 2316 54-55 -0.711035199730297 2316 56-57 -0.8607944061415651 2316 58-59 -0.8138683648781253 2316 60-61 -0.9717799282521682 2316 62-63 -1.0280026568812985 2316 64-65 -0.87534990871265 2316 66-67 -1.0649998759467678 2316 68-69 -1.3290546448381555 2316 70-71 -1.2820361557108662 2316 72-73 -1.4635762026617485 2316 74-75 -1.5872792040411952 2316 76-77 -1.6051600021544274 2316 78-79 -2.105672110414435 2316 80-81 -2.1075204169316564 2316 82-83 -2.0765721855063752 2316 84-85 -1.9431170482513132 2316 86-87 -2.0551257668098195 2316 88-89 -2.5663120121115774 2316 90-91 -2.5111973770581635 2316 92-93 -2.8119836297449154 2316 94-95 -3.0250276122004394 2316 96-97 -3.230987815407989 2316 98-99 -3.349022513166098 2316 100-101 -3.207975444337647 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6301.0 3 2007.0 4 2037.0 5 2266.0 6 2573.0 7 3333.0 8 3919.0 9 4904.0 10 6107.0 11 7649.0 12 9550.0 13 11548.0 14 13480.0 15 16115.0 16 19243.0 17 24417.0 18 23795.0 19 20090.0 20 20599.0 21 22291.0 22 24639.0 23 27627.0 24 31191.0 25 35470.0 26 40154.0 27 46208.0 28 53289.0 29 62401.0 30 73859.0 31 88887.0 32 109646.0 33 137923.0 34 181193.0 35 246466.0 36 332466.0 37 390280.0 38 214758.0 39 11807.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.80850151204571 18.557230337478256 14.110845991192846 23.523422159283186 2 15.606286300552478 19.556473249739213 40.321827337079476 24.515413112628835 3 19.4441474963411 23.553193709050678 29.286753552966164 27.71590524164206 4 12.93447109966189 16.22240111629904 36.42333494338003 34.41979284065905 5 14.249179187325664 36.77584352057239 33.81314459831178 15.161832693790167 6 32.8762499291399 35.25298342475001 17.185376881228173 14.68538976488191 7 29.50849863890412 32.15324467142016 19.980226438616743 18.358030251058977 8 26.540115904700578 35.105430349860484 19.31899549259498 19.03545825284396 9 26.552838694527214 16.801717894548855 20.09575149944427 36.54969191147966 10-11 25.528943482551224 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22.389145489347463 36-37 22.75171922618401 26.127623056841898 28.395426100641757 22.725231616332337 38-39 22.41227050794977 26.391073403333653 28.570143998756997 22.626512089959576 40-41 22.646092608584432 26.256109725257655 28.54145170677439 22.556345959383524 42-43 22.574076311482216 26.167496061545688 28.45148839344327 22.806939233528826 44-45 22.53513268892021 26.47843114366341 28.315495634257392 22.67094053315898 46-47 22.58525370247686 26.684552126328775 27.88638033191756 22.843813839276805 48-49 22.7856636688787 26.554373733007875 27.888548902086807 22.771413696026617 50-51 22.65706877061612 26.75235419686931 27.74714238996367 22.843434642550903 52-53 22.816538249514025 26.829346275258825 27.582339224278428 22.771776250948726 54-55 22.65381279427524 26.64672894468838 27.75902551361772 22.94043274741866 56-57 22.60888970906953 26.989262309504703 27.784417903017456 22.617430078408315 58-59 22.7905388847904 26.5588957168429 27.763272130660688 22.88729326770601 60-61 22.762427738222456 26.623170119352636 27.89143673795208 22.722965404472827 62-63 22.581349133544098 26.709935279476742 27.832228335705555 22.876487251273605 64-65 22.509556078240852 26.516317273170685 27.841282300917808 23.132844347670652 66-67 22.457431371047047 26.713511094117347 27.76787547690361 23.061182057932 68-69 22.44653387780956 26.570552922604396 27.844654761059235 23.138258438526808 70-71 22.41421303283369 26.629393907075467 27.84435654383065 23.112036516260197 72-73 22.716257715117198 26.53787877649931 27.576832514013976 23.169030994369518 74-75 22.520577010311357 26.71005158897564 27.597205157266767 23.172166243446235 76-77 22.634277218549386 26.674775973079218 27.529686262535968 23.161260545835425 78-79 22.726790899438747 26.683502550488342 27.45783304193478 23.13187350813813 80-81 22.483237606472258 26.938667428876506 27.53358594409517 23.044509020556063 82-83 22.74666489280664 26.853808899484367 27.315370139498356 23.08415606821064 84-85 22.823440905391422 26.553719474657374 27.334562282781828 23.288277337169383 86-87 22.770836511454213 26.43520720704484 27.53418799842859 23.259768283072358 88-89 22.81663027207417 26.48580610892624 27.46037997027626 23.237183648723327 90-91 22.794572609409403 26.602807269515 27.462853073666306 23.139767047409293 92-93 22.949935999708163 26.193039472032464 27.399592501075613 23.45743202718376 94-95 22.833019949336315 26.258345311033526 27.390669906315317 23.51796483331484 96-97 23.08466876581659 26.189914122563344 27.19957919712666 23.525837914493405 98-99 22.98636344517437 26.097680000206015 26.987498251008407 23.928458303611205 100-101 23.72889149798058 25.305942741133503 27.08190618787351 23.883259573012403 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 793.0 1 565.5 2 444.5 3 572.0 4 465.0 5 433.0 6 935.0 7 1989.5 8 3219.5 9 3792.5 10 3199.5 11 2489.0 12 2423.0 13 2592.0 14 2701.0 15 2774.0 16 2872.0 17 2967.5 18 3105.5 19 3271.0 20 3552.0 21 4117.0 22 4924.5 23 5886.0 24 7079.5 25 8784.0 26 11338.0 27 14177.5 28 16411.5 29 19226.5 30 22853.5 31 26039.5 32 30099.5 33 34352.5 34 37955.0 35 41705.0 36 46026.0 37 50929.5 38 55214.5 39 58011.5 40 61188.0 41 67076.5 42 73113.0 43 78423.5 44 84093.0 45 88467.5 46 89905.0 47 91452.5 48 94952.0 49 95806.0 50 93765.5 51 90668.0 52 87328.0 53 85003.5 54 83027.0 55 77929.5 56 70087.5 57 64102.5 58 58781.5 59 51721.0 60 43654.0 61 35658.5 62 28493.5 63 22325.5 64 17680.5 65 14120.0 66 10633.0 67 8314.5 68 6265.5 69 4181.5 70 3065.5 71 2322.5 72 1847.0 73 1484.5 74 1174.5 75 919.0 76 632.0 77 360.0 78 185.0 79 126.0 80 83.5 81 50.5 82 29.0 83 22.0 84 18.0 85 12.0 86 8.5 87 8.0 88 8.5 89 6.0 90 4.0 91 2.0 92 1.0 93 2.0 94 2.0 95 1.0 96 0.5 97 2.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.462692792239222 2 0.04196545959472866 3 0.05307901177779075 4 0.05848560473171284 5 0.08594766418020604 6 0.08393091918945732 7 0.04784405669542173 8 0.042394554273611364 9 0.04827315137430444 10-11 0.051426997264092326 12-13 0.04602040431017023 14-15 0.039541074659041366 16-17 0.04106436076907498 18-19 0.049152795466013983 20-21 0.05799214585099773 22-23 0.04932443333756707 24-25 0.0675180477221938 26-27 0.058850335208763135 28-29 0.04670695579638256 30-31 0.048895338658684354 32-33 0.04730768834681835 34-35 0.05181318247508677 36-37 0.04707168627343286 38-39 0.0275264236503256 40-41 0.025574042861409286 42-43 0.024737308237588004 44-45 0.015340134770056742 46-47 0.02842752247597928 48-49 0.028277339338370334 50-51 0.01690633034797862 52-53 0.01658450933881659 54-55 0.017120877687419975 56-57 0.01621977886176629 58-59 0.015490317907665689 60-61 0.017936157577297118 62-63 0.016841966146146217 64-65 0.018300888054347413 66-67 0.00950444713725194 68-69 0.012765566696760508 70-71 0.019631081558883805 72-73 0.017313970292917192 74-75 0.014546309614123737 76-77 0.010448455430793894 78-79 0.014846675889341633 80-81 0.01806488598096193 82-83 0.015147042164559527 84-85 0.013452118182972837 86-87 0.004355310990659467 88-89 0.030058082255733563 90-91 0.033748296494124835 92-93 0.018043431247017794 94-95 0.0348210331913316 96-97 0.028148610934705522 98-99 0.02463003456786733 100-101 0.15370171397578533 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2330488.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.20170052974592 #Duplication Level Percentage of deduplicated Percentage of total 1 76.81959421762598 40.86933046382739 2 12.766061672822099 13.58352380123497 3 4.300794955867631 6.864288158457345 4 1.9198013001680285 4.0854677539262525 5 1.0583756172073358 2.8153691317324836 6 0.659283323802661 2.104499637432281 7 0.43052771183747335 1.6033364476453804 8 0.30303114345685606 1.2897417716302504 9 0.2336940708365834 1.1189629775002623 >10 1.3256406423289062 13.128211561472355 >50 0.11552305152113482 4.283352138238379 >100 0.06466915740067217 6.146049856969045 >500 0.0020291453545661794 0.6757487550224152 >1k 8.116581418264014E-4 0.7036440643548755 >5k 1.6233162836528026E-4 0.7284734805564129 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9372 0.40214753304887213 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7498 0.3217351902262531 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4635 0.1988853836621343 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 8.581893577654123E-5 0.0 0.0 0.0 0.0 8 8.581893577654123E-5 0.0 0.0 0.0 0.0 9 8.581893577654123E-5 0.0 0.0 0.0 0.0 10-11 8.581893577654123E-5 0.0 0.0 0.0 0.0 12-13 8.581893577654123E-5 0.0 0.0 0.0 0.0 14-15 1.2872840366481183E-4 0.0 0.0 0.0 0.0 16-17 1.2872840366481183E-4 0.0 0.0 0.0 0.0 18-19 1.2872840366481183E-4 0.0 0.0 0.0 0.0 20-21 1.2872840366481183E-4 0.0 0.0 4.2909467888270614E-5 0.0 22-23 1.7163787155308245E-4 0.0 0.0 2.1454733944135305E-4 0.0 24-25 1.7163787155308245E-4 0.0 0.0 5.792778164916532E-4 2.1454733944135307E-5 26-27 1.7163787155308245E-4 0.0 0.0 0.0011156461650950358 4.2909467888270614E-5 28-29 2.7891154127375895E-4 0.0 0.0 0.004119308917273979 4.2909467888270614E-5 30-31 3.0036627521789425E-4 0.0 0.0 0.01362375605452592 4.2909467888270614E-5 32-33 3.0036627521789425E-4 0.0 0.0 0.033598113356515885 4.2909467888270614E-5 34-35 3.432757431061649E-4 0.0 0.0 0.06318419146547848 4.2909467888270614E-5 36-37 3.432757431061649E-4 0.0 0.0 0.11495446447267696 4.2909467888270614E-5 38-39 3.647304770503002E-4 0.0 0.0 0.18732128206624535 4.2909467888270614E-5 40-41 3.861852109944355E-4 0.0 0.0 0.26919254679706567 4.2909467888270614E-5 42-43 3.861852109944355E-4 0.0 0.0 0.3476095993628802 4.2909467888270614E-5 44-45 4.720041467709767E-4 0.0 0.0 0.44265407073539964 4.2909467888270614E-5 46-47 5.578230825475179E-4 0.0 0.0 0.5569863479236967 4.2909467888270614E-5 48-49 6.007325504357885E-4 0.0 0.0 0.6742150141944521 4.2909467888270614E-5 50-51 6.007325504357885E-4 0.0 0.0 0.7934818801899002 4.2909467888270614E-5 52-53 6.007325504357885E-4 0.0 0.0 0.9098738118368341 4.2909467888270614E-5 54-55 6.436420183240592E-4 0.0 0.0 1.0356843716852435 4.2909467888270614E-5 56-57 6.436420183240592E-4 0.0 0.0 1.1816838361750843 4.2909467888270614E-5 58-59 6.436420183240592E-4 0.0 0.0 1.3402772294901326 4.2909467888270614E-5 60-61 6.436420183240592E-4 0.0 0.0 1.5034190264013374 4.2909467888270614E-5 62-63 6.865514862123298E-4 0.0 0.0 1.6629778827438717 4.2909467888270614E-5 64-65 6.865514862123298E-4 0.0 0.0 1.8287371571962612 4.2909467888270614E-5 66-67 6.865514862123298E-4 0.0 0.0 2.0128402291708865 6.436420183240593E-5 68-69 7.294609541006004E-4 0.0 0.0 2.215673283878741 8.581893577654123E-5 70-71 7.509156880447358E-4 0.0 0.0 2.4333959239438263 8.581893577654123E-5 72-73 7.72370421988871E-4 0.0 0.0 2.650668014596085 8.581893577654123E-5 74-75 7.72370421988871E-4 0.0 0.0 2.8713728626794044 1.2872840366481183E-4 76-77 7.72370421988871E-4 0.0 0.0 3.101968343110971 1.2872840366481183E-4 78-79 7.72370421988871E-4 0.0 0.0 3.355992393009533 1.2872840366481183E-4 80-81 8.581893577654122E-4 0.0 0.0 3.62612894810014 2.1454733944135305E-4 82-83 8.581893577654122E-4 0.0 0.0 3.9025517402363796 2.1454733944135305E-4 84-85 9.225535595978182E-4 0.0 0.0 4.16605878253825 2.1454733944135305E-4 86-87 9.440082935419534E-4 0.0 0.0 4.446407790986266 2.1454733944135305E-4 88-89 9.440082935419534E-4 0.0 0.0 4.746173333653724 2.1454733944135305E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4155 0.0 54.48426 1 GTATCAA 7610 0.0 43.492294 1 ATCAACG 9365 0.0 34.61349 3 TCAACGC 9435 0.0 34.255936 4 TATCAAC 9540 0.0 34.128014 2 CAACGCA 9535 0.0 33.946518 5 AACGCAG 9935 0.0 32.765503 6 ACGCAGA 11600 0.0 27.969778 7 CGCAGAG 11675 0.0 27.708721 8 GTACATG 8505 0.0 26.617529 1 TGGTATC 2040 0.0 26.094961 2 TACATGG 8565 0.0 25.637957 2 GCAGAGT 12620 0.0 25.142902 9 GTGGTAT 2280 0.0 25.136808 1 ACATGGG 8760 0.0 23.710794 3 GAGTACT 7640 0.0 23.472492 12-13 GTACTTT 8180 0.0 21.664188 14-15 CAGAGTA 12435 0.0 21.449457 10-11 CATGGGG 5170 0.0 21.420725 4 AGTACTT 7910 0.0 20.329084 12-13 >>END_MODULE