FastQCFastQC Report
Thu 26 May 2016
SRR938154_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938154_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2330488
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93040.39922968923246976No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77810.3338785696386336No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49190.21107167254240314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36900.056.7738721
GTATCAA71450.046.4076351
TCAACGC89300.036.593064
ATCAACG89500.036.2482763
CAACGCA91200.035.8307045
TATCAAC92450.035.5064662
AACGCAG94350.034.634456
ACGCAGA110650.029.2748537
CGCAGAG112200.028.8704288
GCAGAGT123850.025.962979
GTACATG84850.024.9140571
GTGGTAT17600.024.8319841
TACATGG86250.024.0139542
CCGTGCG1852.5457666E-723.1064139
TGGTATC17400.022.9332892
GAGTACT71100.022.91322712-13
ACATGGG91200.021.978973
AGTACTT74050.021.39107112-13
CAGAGTA121050.021.2665110-11
GTACTTT76500.021.17164814-15
Produced by FastQC (version 0.11.5)