##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938154_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2330488 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.504856493575595 34.0 31.0 34.0 31.0 34.0 2 32.73522584111139 34.0 31.0 34.0 31.0 34.0 3 32.70172212858423 34.0 31.0 34.0 31.0 34.0 4 36.220006711040774 37.0 35.0 37.0 35.0 37.0 5 36.17869819539942 37.0 35.0 37.0 35.0 37.0 6 36.239465296538754 37.0 37.0 37.0 35.0 37.0 7 36.19865281434618 37.0 37.0 37.0 35.0 37.0 8 36.19800101952896 37.0 37.0 37.0 35.0 37.0 9 37.903796543899816 39.0 38.0 39.0 35.0 39.0 10-11 37.869709262609376 39.0 38.0 39.0 35.0 39.0 12-13 37.81159117747013 39.0 38.0 39.0 35.0 39.0 14-15 39.10939511381307 40.0 38.5 41.0 36.0 41.0 16-17 39.045732696327974 40.0 38.0 41.0 36.0 41.0 18-19 39.02740563349822 40.0 38.5 41.0 36.0 41.0 20-21 39.03086284932598 40.0 38.5 41.0 35.5 41.0 22-23 39.05537059191036 40.0 39.0 41.0 35.5 41.0 24-25 39.01021223881007 40.0 38.5 41.0 35.0 41.0 26-27 38.85277225199186 40.0 38.0 41.0 35.0 41.0 28-29 38.77614259331093 40.0 38.0 41.0 35.0 41.0 30-31 38.587104717981816 40.0 38.0 41.0 34.5 41.0 32-33 38.41947995441298 40.0 38.0 41.0 34.0 41.0 34-35 38.22045082403342 40.0 38.0 41.0 34.0 41.0 36-37 38.16239345579123 40.0 38.0 41.0 34.0 41.0 38-39 38.146018988297726 40.0 38.0 41.0 34.0 41.0 40-41 38.03903731750603 40.0 38.0 41.0 33.5 41.0 42-43 37.86838743645108 40.0 37.5 41.0 33.0 41.0 44-45 37.63794407008318 40.0 37.0 41.0 33.0 41.0 46-47 37.29854026281191 40.0 37.0 41.0 32.5 41.0 48-49 36.9631718764482 40.0 36.5 41.0 31.0 41.0 50-51 36.691456896581315 39.5 36.0 41.0 31.0 41.0 52-53 36.24783907919715 39.0 35.0 40.5 30.0 41.0 54-55 35.940469120630524 39.0 35.0 40.0 29.0 41.0 56-57 35.68211807999011 38.5 35.0 40.0 28.0 41.0 58-59 35.31431893234378 38.0 34.5 40.0 28.0 41.0 60-61 34.909180180288416 38.0 34.0 40.0 27.0 41.0 62-63 34.48463047224444 37.0 34.0 40.0 26.0 41.0 64-65 34.04338275931908 36.5 33.5 39.5 26.0 41.0 66-67 33.52968884628456 36.0 33.0 39.0 25.0 40.0 68-69 33.00307896886832 35.5 32.0 39.0 23.5 40.0 70-71 32.416307657452 35.0 31.5 38.0 22.0 40.0 72-73 31.93675230252205 35.0 31.0 37.0 20.5 39.0 74-75 31.367136840009472 34.5 30.5 36.5 19.5 39.0 76-77 30.142345723299155 33.5 29.5 35.0 19.0 37.5 78-79 30.842671792345637 34.0 30.5 36.0 19.5 38.0 80-81 30.646465890405786 34.0 31.0 35.5 18.0 37.0 82-83 30.19020093645623 34.0 30.0 35.0 14.0 37.0 84-85 29.685040858395325 34.0 29.5 35.0 7.5 36.0 86-87 29.13082131296106 34.0 29.0 35.0 4.5 36.0 88-89 28.525630468811684 33.5 28.5 35.0 2.0 35.5 90-91 27.998596860400056 33.0 27.0 35.0 2.0 35.0 92-93 27.3574195619115 33.0 26.0 35.0 2.0 35.0 94-95 26.60443091747308 32.5 25.0 34.5 2.0 35.0 96-97 25.781013461558267 32.0 23.5 34.0 2.0 35.0 98-99 24.750247587629715 32.0 11.0 34.0 2.0 35.0 100-101 21.215192268743714 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.00756698413904644 1101 2 0.0534488520423011 1101 3 0.15338057926057758 1101 4 0.049398700467783385 1101 5 0.07904485121343185 1101 6 0.06567867017649576 1101 7 0.05987958622898759 1101 8 0.06935086471570884 1101 9 0.021068449945538248 1101 10-11 0.11145642235203468 1101 12-13 0.0808375264278709 1101 14-15 0.1951275725878716 1101 16-17 0.20890889297486126 1101 18-19 0.19161493445671596 1101 20-21 0.1706821964374612 1101 22-23 0.1803787010550124 1101 24-25 0.22346150327470582 1101 26-27 0.27962226428296333 1101 28-29 0.19492510717684297 1101 30-31 0.12867448154486993 1101 32-33 0.23095298892230431 1101 34-35 0.17056289248077405 1101 36-37 0.17305867059288715 1101 38-39 0.2005385314453676 1101 40-41 0.20789580684000697 1101 42-43 0.18679043139284346 1101 44-45 0.41677202018550474 1101 46-47 0.37937114631012037 1101 48-49 0.30001072257162775 1101 50-51 0.5799953660287542 1101 52-53 0.40587002856224075 1101 54-55 0.5008260488359113 1101 56-57 0.5187455848939706 1101 58-59 0.6251629424298102 1101 60-61 0.6512767156268779 1101 62-63 0.633700820976216 1101 64-65 0.7093592145985212 1101 66-67 0.639738090365384 1101 68-69 0.6793887964141234 1101 70-71 0.5698154795603543 1101 72-73 0.6386123781695972 1101 74-75 0.709354936879329 1101 76-77 0.7650228679827542 1101 78-79 0.8894514792345198 1101 80-81 1.0301005219377046 1101 82-83 0.9916077027878494 1101 84-85 1.2017201270549727 1101 86-87 1.1334208294352628 1101 88-89 1.4783005471954702 1101 90-91 1.6268524254364891 1101 92-93 1.9027655054631971 1101 94-95 1.9254312410126424 1101 96-97 2.2565859089911946 1101 98-99 2.4239858666930374 1101 100-101 2.9512937809715076 1102 1 0.11722182169572903 1102 2 0.1439674643797062 1102 3 0.26698453397046507 1102 4 0.11505619050901572 1102 5 0.08179149386680251 1102 6 0.08439146970209066 1102 7 0.1453994960327094 1102 8 0.0630268349073333 1102 9 0.07043236606494929 1102 10-11 0.1304079719498219 1102 12-13 0.2322384239979911 1102 14-15 0.2612719175463454 1102 16-17 0.3026949647755899 1102 18-19 0.33058994826516397 1102 20-21 0.3286132338655037 1102 22-23 0.3473939346924979 1102 24-25 0.27353221315554066 1102 26-27 0.20628928480407893 1102 28-29 0.1608623455990852 1102 30-31 0.29418573208276655 1102 32-33 0.3441793972020122 1102 34-35 0.37456181383479503 1102 36-37 0.4523377877301016 1102 38-39 0.4500072413571061 1102 40-41 0.4266004311075875 1102 42-43 0.4134228189179723 1102 44-45 0.5237351029980317 1102 46-47 0.44942843005828337 1102 48-49 0.5324870977028979 1102 50-51 0.44788106127953853 1102 52-53 0.5966473003688293 1102 54-55 0.5014459042192598 1102 56-57 0.4920442160873364 1102 58-59 0.4616238203630374 1102 60-61 0.6783502417010681 1102 62-63 0.709746887945002 1102 64-65 0.735223534600081 1102 66-67 0.7423216142513667 1102 68-69 0.8262046351020444 1102 70-71 1.0884738790482018 1102 72-73 1.1243115742285568 1102 74-75 1.0565339441182928 1102 76-77 0.9354637787634417 1102 78-79 1.053359199687879 1102 80-81 1.309448681365808 1102 82-83 1.2783089170373678 1102 84-85 1.3583423097431115 1102 86-87 1.5623854635131558 1102 88-89 1.6469758462838264 1102 90-91 1.7594118953543862 1102 92-93 1.8983325633220574 1102 94-95 1.9920550180794585 1102 96-97 2.1897743246912675 1102 98-99 2.5514998602130277 1102 100-101 2.981692115333864 1103 1 0.29188948111838187 1103 2 0.26012611919534834 1103 3 0.3630654746067563 1103 4 0.20950761526896855 1103 5 0.14581960127059546 1103 6 0.1357665918576103 1103 7 0.1943477951624999 1103 8 0.22005756380739427 1103 9 0.22418312226579928 1103 10-11 0.27838290486123185 1103 12-13 0.2635020937825274 1103 14-15 0.4540434130401607 1103 16-17 0.40736246908011964 1103 18-19 0.4558590782076166 1103 20-21 0.4355156025192386 1103 22-23 0.5162597885402036 1103 24-25 0.4961891539159282 1103 26-27 0.4337533361791941 1103 28-29 0.41162087321787055 1103 30-31 0.33310642018675907 1103 32-33 0.40373312236372527 1103 34-35 0.4079405192189398 1103 36-37 0.4211365529898501 1103 38-39 0.4477673356689351 1103 40-41 0.5326011756745714 1103 42-43 0.6013728553707267 1103 44-45 0.600071057827634 1103 46-47 0.6814335644681364 1103 48-49 0.6870937181443608 1103 50-51 0.7463081635178952 1103 52-53 0.6636967210026441 1103 54-55 0.7466783487257516 1103 56-57 0.6958391809530866 1103 58-59 0.7984860909821094 1103 60-61 0.9323080805953836 1103 62-63 0.9930115815091511 1103 64-65 1.127969823398999 1103 66-67 1.0696034018326088 1103 68-69 1.0677952938887145 1103 70-71 1.1697926157662124 1103 72-73 1.0549501179796223 1103 74-75 0.9389183423375442 1103 76-77 0.8617497079985519 1103 78-79 1.1331213260470996 1103 80-81 1.0514833657779903 1103 82-83 1.1337461950245533 1103 84-85 1.4257136004810071 1103 86-87 1.521889163080374 1103 88-89 1.5978371396587328 1103 90-91 1.5953310477889673 1103 92-93 1.8197086161863183 1103 94-95 1.939203559193519 1103 96-97 2.242163531572313 1103 98-99 2.6736293993570897 1103 100-101 3.1116838165143115 1104 1 0.10748139178500793 1104 2 0.0866264901330922 1104 3 0.12797620867811332 1104 4 0.049339585285636645 1104 5 -0.006781298614754405 1104 6 0.050672508555585694 1104 7 0.0855700591352857 1104 8 -0.005828110287659172 1104 9 0.06785887449864703 1104 10-11 0.14207050783259234 1104 12-13 0.08139646805390299 1104 14-15 0.12272663220174707 1104 16-17 0.17237533146703043 1104 18-19 0.12896970436981547 1104 20-21 0.167216568884335 1104 22-23 0.1273163830426185 1104 24-25 -0.01610068817057453 1104 26-27 0.14402662577077763 1104 28-29 0.07774365843046382 1104 30-31 0.12149558687750783 1104 32-33 0.15344407996119713 1104 34-35 0.20478489878941986 1104 36-37 0.020320008202723727 1104 38-39 0.10783853644741725 1104 40-41 0.21099670129818549 1104 42-43 0.19810113624286885 1104 44-45 0.04904110339998624 1104 46-47 0.1604729320433549 1104 48-49 0.1010595244281447 1104 50-51 0.22481448872913745 1104 52-53 0.21869980412160572 1104 54-55 0.32080857196282864 1104 56-57 0.3828337180516712 1104 58-59 0.34232900607108974 1104 60-61 0.4094910733404262 1104 62-63 0.4298324522975605 1104 64-65 0.4289687057683764 1104 66-67 0.4714843452670152 1104 68-69 0.638245766473041 1104 70-71 0.623942060323067 1104 72-73 0.7540745603686503 1104 74-75 0.7607355037484496 1104 76-77 0.6793482955446812 1104 78-79 0.846219523385983 1104 80-81 0.9375693462579946 1104 82-83 0.9772665869660528 1104 84-85 1.2306492403727098 1104 86-87 1.1876720826013347 1104 88-89 1.2810873733013395 1104 90-91 1.3232544708217908 1104 92-93 1.5192148354153439 1104 94-95 1.618535341332624 1104 96-97 1.842604562104821 1104 98-99 2.03265643445647 1104 100-101 2.5829190392214834 1105 1 0.102614268226489 1105 2 0.12014635824525755 1105 3 0.15731262013698455 1105 4 0.09955324859848247 1105 5 0.049738530510289536 1105 6 0.07414727693035417 1105 7 0.07919810256693438 1105 8 0.08824884192249272 1105 9 0.03149782039845661 1105 10-11 0.0351706239584999 1105 12-13 0.052997835944673 1105 14-15 0.17055494547992822 1105 16-17 0.12514801152484267 1105 18-19 0.10270770538904372 1105 20-21 0.14468236465311435 1105 22-23 0.1872072051973177 1105 24-25 0.1360524448618179 1105 26-27 0.1257312259718475 1105 28-29 0.131994054567393 1105 30-31 0.1792285418501791 1105 32-33 0.09036675737345945 1105 34-35 0.14196360319190404 1105 36-37 0.1314226682378674 1105 38-39 0.2246065115959155 1105 40-41 0.22959825437774128 1105 42-43 0.09256075394635843 1105 44-45 0.11450951965983336 1105 46-47 0.1832264387950886 1105 48-49 0.30494942899326105 1105 50-51 0.2538567563311247 1105 52-53 0.20450275071203805 1105 54-55 0.31217745133391617 1105 56-57 0.349518010143143 1105 58-59 0.41007294705573827 1105 60-61 0.40250942095510567 1105 62-63 0.3846023517386499 1105 64-65 0.5729310637090776 1105 66-67 0.6082318243323215 1105 68-69 0.6956163501379962 1105 70-71 0.9064439122110173 1105 72-73 0.7600456806633957 1105 74-75 0.6288532336958497 1105 76-77 0.5847324857126885 1105 78-79 0.8456733556801233 1105 80-81 1.021878981923404 1105 82-83 1.0283417958952157 1105 84-85 1.0878775558787233 1105 86-87 1.1837199378923025 1105 88-89 1.3740147277749735 1105 90-91 1.525720649499739 1105 92-93 1.7188532299259585 1105 94-95 1.7628297859472504 1105 96-97 1.9271408103747696 1105 98-99 2.0943410119893073 1105 100-101 2.5323605836124905 1106 1 -0.003451577860325017 1106 2 0.04159399181641277 1106 3 0.15377114636104494 1106 4 0.01248010443422487 1106 5 -0.003327751282640179 1106 6 -0.012806048051942298 1106 7 0.02304103051203299 1106 8 -0.029579707720778003 1106 9 -0.08538443002363039 1106 10-11 0.07095639270747256 1106 12-13 -0.019138757922760874 1106 14-15 -0.01726570698998131 1106 16-17 0.04847326614060421 1106 18-19 -0.05204557602264259 1106 20-21 -0.03798402062002282 1106 22-23 0.0029259841309325907 1106 24-25 0.08665222156243146 1106 26-27 0.06917589129508883 1106 28-29 -2.5024040564858296E-4 1106 30-31 0.022224566031873394 1106 32-33 0.05215737335297632 1106 34-35 -0.029432290277945583 1106 36-37 0.1679709226902446 1106 38-39 0.196038486978523 1106 40-41 0.1382470207848101 1106 42-43 0.09870421018512587 1106 44-45 0.21064671262318768 1106 46-47 -0.08595620376250679 1106 48-49 0.16991593408557293 1106 50-51 0.14633878345382811 1106 52-53 0.10897418472979581 1106 54-55 0.03538901388497351 1106 56-57 0.2507713460180909 1106 58-59 0.18966283867383993 1106 60-61 0.14906189631047795 1106 62-63 0.09515733240419166 1106 64-65 0.2475736485356137 1106 66-67 0.3972956008150845 1106 68-69 -0.005904536523949844 1106 70-71 0.04134669528383483 1106 72-73 0.10177271078158157 1106 74-75 0.43519684243175405 1106 76-77 0.6518877332482234 1106 78-79 0.6164687916585585 1106 80-81 0.7384439838296188 1106 82-83 0.7515104686254581 1106 84-85 0.871914373045545 1106 86-87 1.065449555015551 1106 88-89 1.0503409082662323 1106 90-91 1.016046612137938 1106 92-93 0.9264685558885972 1106 94-95 1.202400751580928 1106 96-97 1.3931339480034381 1106 98-99 1.8404986286768548 1106 100-101 2.5128143987641707 1107 1 0.06859261513019987 1107 2 0.059163344952281705 1107 3 0.17380971726219485 1107 4 0.03428270303129466 1107 5 0.03007915067408362 1107 6 0.06028872431173227 1107 7 0.0022786479897405343 1107 8 -0.008361987775963087 1107 9 0.07296239718780129 1107 10-11 0.029374062778920518 1107 12-13 0.0466066546984365 1107 14-15 0.12089900144508903 1107 16-17 0.11096606040970158 1107 18-19 0.0890081447253337 1107 20-21 0.08922830069405308 1107 22-23 0.13631353586645645 1107 24-25 0.1363056178096187 1107 26-27 0.11444755544648899 1107 28-29 0.03316135742065285 1107 30-31 0.03365819882134247 1107 32-33 0.011421919213809417 1107 34-35 0.015761811062155573 1107 36-37 -0.04709666544637514 1107 38-39 -0.005309227590331034 1107 40-41 0.0687140654959606 1107 42-43 0.006154369196984533 1107 44-45 0.047190455457936764 1107 46-47 0.09504989439088263 1107 48-49 0.14278214714203585 1107 50-51 0.0369242936195775 1107 52-53 0.058907713886284796 1107 54-55 -0.008276986723871005 1107 56-57 0.04942233603694035 1107 58-59 0.12718720861768418 1107 60-61 0.2567089579915347 1107 62-63 0.34526217983700747 1107 64-65 0.11666664942296734 1107 66-67 0.31249669738159724 1107 68-69 0.4019398326426469 1107 70-71 0.4747348947255077 1107 72-73 0.44484314558671656 1107 74-75 0.350441092420418 1107 76-77 0.4792916136557608 1107 78-79 0.6226158468625513 1107 80-81 0.6352912741509442 1107 82-83 0.7716421472854869 1107 84-85 0.7173116921897957 1107 86-87 0.8669502901620341 1107 88-89 0.9381940700437958 1107 90-91 1.0834748462966672 1107 92-93 1.1979932885478846 1107 94-95 1.214066182208363 1107 96-97 1.3029539828500312 1107 98-99 1.5924744562833872 1107 100-101 2.0254500122390517 1108 1 0.14249362047616643 1108 2 0.06933757242916272 1108 3 0.11780763417088735 1108 4 0.090414136407162 1108 5 0.06535269740678018 1108 6 0.08772079318983828 1108 7 0.05711274705427627 1108 8 0.06168655536281875 1108 9 0.11086218382113344 1108 10-11 0.07452934289124613 1108 12-13 0.0738199468808105 1108 14-15 0.1876950899170069 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-3.719813591021996 2316 100-101 -4.375387825196846 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 6.0 4 13.0 5 48.0 6 92.0 7 207.0 8 420.0 9 829.0 10 1474.0 11 2374.0 12 3614.0 13 5521.0 14 7506.0 15 9849.0 16 13148.0 17 18765.0 18 19199.0 19 14232.0 20 13086.0 21 13869.0 22 14553.0 23 16022.0 24 18704.0 25 21701.0 26 25719.0 27 31485.0 28 38032.0 29 46335.0 30 56993.0 31 69935.0 32 87724.0 33 114722.0 34 159963.0 35 235569.0 36 365090.0 37 528906.0 38 353074.0 39 21706.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.04248723872425 18.993361047128328 14.475165716364986 23.48898599778244 2 15.872342872542852 19.831888705121614 39.73342682862982 24.56234159370572 3 19.72315592122513 23.507715836808153 29.416989903177093 27.35213833878963 4 13.223716759630069 16.5201522256936 36.12536778793445 34.13076322674188 5 14.28535874844506 36.780152732680904 33.89164535843949 15.042843160434538 6 32.9960643368502 35.44212275218367 16.976731013815154 14.585081897150978 7 29.45812207571976 32.23415010075143 19.863093051755683 18.44463477177312 8 26.5979714102552 35.30585465864202 19.124766250473076 18.971407680629707 9 26.391210767873506 16.70787405899537 20.088324848701216 36.812590324429905 10-11 25.532485041759497 26.44074974855052 26.564479199206346 21.46228601048364 12-13 25.887732526406488 23.59254799853078 27.558219566030807 22.96149990903193 14-15 22.745386374012654 25.091204073996522 25.67470847307517 26.48870107891566 16-17 22.70703018145345 28.493780807663633 26.181110175446126 22.61807883543679 18-19 22.4574852992163 26.825325854499145 28.632586823017327 22.08460202326723 20-21 23.02554228985517 26.093097239719746 28.848571629632936 22.032788840792144 22-23 22.872462763163767 25.85123802396751 28.767901830002984 22.508397382865734 24-25 22.642759491911033 26.139561408100896 28.748361909976495 22.46931719001158 26-27 22.437826553831865 26.572093558236134 28.60358349778156 22.386496390150445 28-29 22.240961792659586 26.526406667463355 28.927326820659164 22.305304719217894 30-31 22.41597519832224 26.261522535521003 28.818519730313934 22.503982535842823 32-33 22.257983608502432 26.334853162451072 28.72584309536218 22.681320133684316 34-35 22.763293445540146 26.398213171061318 28.486609189963875 22.351884193434664 36-37 22.69596720639651 26.153220624095493 28.436049362054554 22.714762807453443 38-39 22.359665860673992 26.42340537482951 28.599306623131042 22.617622141365448 40-41 22.567680982801026 26.247991678946743 28.637182893428676 22.54714444482356 42-43 22.540087455736746 26.159342137845996 28.504205626127373 22.796364780289885 44-45 22.511022214364367 26.423747389814334 28.371484456467726 22.693745939353573 46-47 22.549409166860478 26.739286777850346 27.895292644558662 22.816011410730518 48-49 22.717276754528243 26.562958595725178 27.979531290495878 22.7402333592507 50-51 22.599258181119147 26.7373185358603 27.854831262808478 22.808592020212075 52-53 22.823331422431696 26.766818795033487 27.621296483826562 22.78855329870825 54-55 22.61944708576058 26.691190857880407 27.74273027795037 22.94663177840864 56-57 22.587479532183817 27.010136932693925 27.766180302151312 22.636203232970946 58-59 22.78398344037815 26.617858577259355 27.721618819749338 22.876539162613152 60-61 22.713011180490952 26.67578635890852 27.86465753095489 22.746544929645637 62-63 22.53109649137863 26.753066310575296 27.784588463875377 22.931248734170698 64-65 22.472803979252415 26.535794219923037 27.753028550243553 23.23837325058099 66-67 22.37983642422085 26.840098786537276 27.673060415249306 23.107004373992563 68-69 22.32539119606639 26.62724991106986 27.830241911433735 23.217116981430014 70-71 22.313330941845656 26.749676462612122 27.706579051254497 23.23041354428772 72-73 22.601746930256667 26.640686414175914 27.45309136970454 23.304475285862875 74-75 22.453880903913685 26.884433646515237 27.326808805709362 23.334876643861715 76-77 22.444269183106712 26.75463250615322 27.48825567863898 23.312842632101088 78-79 22.437746943987698 26.84534312126902 27.435434123668518 23.281475811074763 80-81 22.24263526657474 27.021433124442307 27.558875708732295 23.177055900250657 82-83 22.46349759682947 26.972022297715085 27.36932532290794 23.195154782547505 84-85 22.45274440645121 26.689232318000606 27.363970467547105 23.49405280800108 86-87 22.410777577722584 26.613796027235733 27.505290782795562 23.470135612246125 88-89 22.383132795849107 26.764126982900997 27.344456330372154 23.508283890877742 90-91 22.33654731404573 26.83905257979185 27.5063224355221 23.31807767064032 92-93 22.389574991857877 26.482478020556343 27.44805453656833 23.679892451017455 94-95 22.19848610943943 26.58426752708824 27.48023963247143 23.7370067310009 96-97 22.41444266512879 26.52663566881386 27.358072400411242 23.700849265646106 98-99 22.31606775329336 26.46737191343688 27.16480199718127 24.051758336088483 100-101 22.423644407026433 26.267079208823613 27.326305012146907 23.982971372003046 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 822.0 1 560.0 2 434.0 3 596.5 4 481.0 5 457.0 6 951.5 7 2060.5 8 3328.0 9 3910.0 10 3393.0 11 2684.5 12 2542.5 13 2637.5 14 2738.0 15 2769.0 16 2842.0 17 2915.5 18 3025.5 19 3221.5 20 3501.0 21 3971.5 22 4722.5 23 5751.5 24 6904.5 25 8605.0 26 11063.0 27 13859.5 28 16126.5 29 18862.5 30 22723.5 31 26134.5 32 29950.0 33 34016.0 34 37632.5 35 41504.0 36 46016.5 37 51094.0 38 55559.5 39 58077.0 40 61177.0 41 67540.0 42 73607.5 43 79084.0 44 85200.0 45 89467.5 46 90563.5 47 92485.5 48 96302.0 49 96870.0 50 95217.5 51 91891.5 52 87371.5 53 85073.5 54 84087.5 55 78677.5 56 69890.5 57 63714.5 58 58982.5 59 51976.5 60 43092.5 61 34611.0 62 27493.0 63 21597.5 64 17030.0 65 13079.0 66 9347.0 67 7500.0 68 5654.5 69 3562.0 70 2568.5 71 1984.5 72 1656.0 73 1296.0 74 970.0 75 785.5 76 499.0 77 245.5 78 126.5 79 87.0 80 42.5 81 16.5 82 13.0 83 6.5 84 4.0 85 4.0 86 1.0 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01055572910051457 3 0.0064793296511288625 4 4.2909467888270614E-5 5 4.720041467709767E-4 6 1.7163787155308245E-4 7 0.0 8 8.581893577654123E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.1454733944135307E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0012872840366481184 26-27 0.02033908777904027 28-29 2.7891154127375895E-4 30-31 2.1454733944135307E-5 32-33 0.0031109364218996193 34-35 0.014009941265520355 36-37 0.00667242225662608 38-39 0.01896598480661561 40-41 0.021218731870749817 42-43 0.016949239815866893 44-45 0.008324436770324498 46-47 0.0021454733944135307 48-49 1.7163787155308245E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.647304770503002E-4 68-69 3.0036627521789425E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.7891154127375895E-4 82-83 0.0010727366972067653 84-85 0.0043982204585477375 86-87 8.581893577654122E-4 88-89 4.2909467888270614E-5 90-91 0.004140763651218114 92-93 6.436420183240592E-4 94-95 1.0727366972067653E-4 96-97 1.7163787155308245E-4 98-99 1.5018313760894713E-4 100-101 0.0025531133393521016 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2330488.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.47310606951907 #Duplication Level Percentage of deduplicated Percentage of total 1 75.42830009012985 38.82528891182769 2 13.553865167421517 13.953190788292952 3 4.478610687203071 6.91584008839456 4 2.086591830807623 4.2961345052381095 5 1.1137514122814136 2.8664122289718934 6 0.6749323732843971 2.0844519383892006 7 0.46966913114958275 1.6922730303668934 8 0.3266298571841494 1.3450122627449268 9 0.2553467810866965 1.1829142752647215 >10 1.4132841854441491 13.584152245583562 >50 0.12401584595509701 4.403361407748519 >100 0.07148601151018022 6.573841244080783 >500 0.0025118761017324577 0.8035195160649578 >1k 8.372920339106832E-4 0.7372778567539885 >5k 1.6745840678213664E-4 0.7363297002771902 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9304 0.39922968923246976 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7781 0.3338785696386336 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4919 0.21107167254240314 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 4.2909467888270614E-5 0.0 0.0 0.0 4.2909467888270614E-5 8 4.2909467888270614E-5 0.0 0.0 0.0 4.2909467888270614E-5 9 4.2909467888270614E-5 0.0 0.0 0.0 4.2909467888270614E-5 10-11 4.2909467888270614E-5 0.0 0.0 0.0 8.581893577654123E-5 12-13 4.2909467888270614E-5 0.0 0.0 0.0 8.581893577654123E-5 14-15 8.581893577654123E-5 0.0 0.0 0.0 8.581893577654123E-5 16-17 1.0727366972067653E-4 0.0 0.0 0.0 8.581893577654123E-5 18-19 1.2872840366481183E-4 0.0 0.0 0.0 8.581893577654123E-5 20-21 1.2872840366481183E-4 0.0 0.0 4.2909467888270614E-5 8.581893577654123E-5 22-23 1.2872840366481183E-4 0.0 0.0 2.1454733944135305E-4 8.581893577654123E-5 24-25 1.7163787155308245E-4 0.0 0.0 5.792778164916532E-4 8.581893577654123E-5 26-27 1.7163787155308245E-4 0.0 0.0 0.0010941914311509006 8.581893577654123E-5 28-29 1.7163787155308245E-4 0.0 0.0 0.004054944715441573 8.581893577654123E-5 30-31 1.7163787155308245E-4 0.0 0.0 0.01366666552241419 8.581893577654123E-5 32-33 1.7163787155308245E-4 0.0 0.0 0.033705387026236565 8.581893577654123E-5 34-35 2.1454733944135305E-4 0.0 0.0 0.06342019353886397 8.581893577654123E-5 36-37 2.5745680732962365E-4 0.0 0.0 0.11549083282128035 8.581893577654123E-5 38-39 2.5745680732962365E-4 0.0 0.0 0.18835110929556387 8.581893577654123E-5 40-41 2.5745680732962365E-4 0.0 0.0 0.27041546663188143 8.581893577654123E-5 42-43 3.003662752178943E-4 0.0 0.0 0.3489612476013607 8.581893577654123E-5 44-45 3.432757431061649E-4 0.0 0.0 0.4441344473775449 8.581893577654123E-5 46-47 3.432757431061649E-4 0.0 0.0 0.5591103665841661 8.581893577654123E-5 48-49 3.432757431061649E-4 0.0 0.0 0.6765535801943627 8.581893577654123E-5 50-51 3.432757431061649E-4 0.0 0.0 0.795841900923755 1.0727366972067653E-4 52-53 3.432757431061649E-4 0.0 0.0 0.9127702009192924 1.2872840366481183E-4 54-55 3.432757431061649E-4 0.0 0.0 1.0387953081071433 1.2872840366481183E-4 56-57 3.861852109944355E-4 0.0 0.0 1.1848591367988164 1.2872840366481183E-4 58-59 3.861852109944355E-4 0.0 0.0 1.3439674437285238 1.2872840366481183E-4 60-61 3.861852109944355E-4 0.0 0.0 1.5079888847314382 1.2872840366481183E-4 62-63 3.861852109944355E-4 0.0 0.0 1.667805197881302 1.2872840366481183E-4 64-65 3.861852109944355E-4 0.0 0.0 1.8337790196731327 1.2872840366481183E-4 66-67 5.149136146592473E-4 0.0 0.0 2.0180108200514226 1.2872840366481183E-4 68-69 5.149136146592473E-4 0.0 0.0 2.221358788373937 1.7163787155308245E-4 70-71 5.149136146592473E-4 0.0 0.0 2.4391028831729664 1.7163787155308245E-4 72-73 5.149136146592473E-4 0.0 0.0 2.656932796907772 1.7163787155308245E-4 74-75 5.149136146592473E-4 0.0 0.0 2.8788176553580196 1.7163787155308245E-4 76-77 5.149136146592473E-4 0.0 0.0 3.112502617477541 1.7163787155308245E-4 78-79 5.149136146592473E-4 0.0 0.0 3.3703670647521036 1.7163787155308245E-4 80-81 6.007325504357885E-4 0.0 0.0 3.645266570778309 1.7163787155308245E-4 82-83 6.865514862123298E-4 0.0 0.0 3.926259221244649 1.7163787155308245E-4 84-85 6.865514862123298E-4 0.0 0.0 4.195902317454542 1.7163787155308245E-4 86-87 6.865514862123298E-4 0.0 0.0 4.482988112361016 1.7163787155308245E-4 88-89 7.294609541006004E-4 0.0 0.0 4.792065009560229 1.7163787155308245E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3690 0.0 56.773872 1 GTATCAA 7145 0.0 46.407635 1 TCAACGC 8930 0.0 36.59306 4 ATCAACG 8950 0.0 36.248276 3 CAACGCA 9120 0.0 35.830704 5 TATCAAC 9245 0.0 35.506466 2 AACGCAG 9435 0.0 34.63445 6 ACGCAGA 11065 0.0 29.274853 7 CGCAGAG 11220 0.0 28.870428 8 GCAGAGT 12385 0.0 25.96297 9 GTACATG 8485 0.0 24.914057 1 GTGGTAT 1760 0.0 24.831984 1 TACATGG 8625 0.0 24.013954 2 CCGTGCG 185 2.5457666E-7 23.106413 9 TGGTATC 1740 0.0 22.933289 2 GAGTACT 7110 0.0 22.913227 12-13 ACATGGG 9120 0.0 21.97897 3 AGTACTT 7405 0.0 21.391071 12-13 CAGAGTA 12105 0.0 21.26651 10-11 GTACTTT 7650 0.0 21.171648 14-15 >>END_MODULE