FastQCFastQC Report
Thu 26 May 2016
SRR938153_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938153_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2381744
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99530.4178870609099886No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78700.3304301385875224No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49410.20745302601790955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41300.058.8409421
GTATCAA74650.046.2402761
ATCAACG96700.035.0383383
TATCAAC98050.034.7490462
TCAACGC98150.034.617544
CAACGCA98450.034.5120545
AACGCAG100650.033.7576946
ACGCAGA118250.028.7490027
CGCAGAG119600.028.2654768
GTACATG87300.025.5943151
GCAGAGT131750.025.3340369
TACATGG87100.025.0418192
GTGGTAT18950.023.9346071
GAGTACT77400.023.79992312-13
TGGTATC17400.023.2135222
ACATGGG90450.023.0640953
CAGAGTA127150.021.98626510-11
GTACTTT84300.021.72334514-15
AGTACTT81400.021.46237412-13
CATGGGG53350.020.4867884
Produced by FastQC (version 0.11.5)