##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938153_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2381744 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.93221647666584 33.0 31.0 34.0 30.0 34.0 2 32.119820182185826 34.0 31.0 34.0 30.0 34.0 3 32.195046570916105 34.0 31.0 34.0 30.0 34.0 4 35.701428449069255 37.0 35.0 37.0 35.0 37.0 5 35.72140414754902 37.0 35.0 37.0 35.0 37.0 6 35.74632412215587 37.0 36.0 37.0 35.0 37.0 7 35.68572357062724 37.0 35.0 37.0 33.0 37.0 8 35.67055023545772 37.0 35.0 37.0 33.0 37.0 9 37.337198288313104 39.0 37.0 39.0 34.0 39.0 10-11 37.259469111709734 39.0 37.0 39.0 34.0 39.0 12-13 37.16366704398122 39.0 37.0 39.0 34.0 39.0 14-15 38.44053538079659 40.0 38.0 41.0 34.0 41.0 16-17 38.36870608260166 40.0 38.0 41.0 34.0 41.0 18-19 38.29560649675196 40.0 38.0 41.0 33.5 41.0 20-21 38.19251334316367 40.0 38.0 41.0 33.5 41.0 22-23 38.04162160164988 40.0 38.0 41.0 33.0 41.0 24-25 37.99963996970287 40.0 38.0 41.0 33.0 41.0 26-27 38.03012099537146 40.0 38.0 41.0 33.0 41.0 28-29 37.937896978012745 40.0 38.0 41.0 33.0 41.0 30-31 37.774577578446724 40.0 38.0 41.0 33.0 41.0 32-33 37.5860547985006 40.0 37.0 41.0 32.5 41.0 34-35 37.39041937336674 40.0 37.0 41.0 32.0 41.0 36-37 37.19441950940151 40.0 37.0 41.0 31.0 41.0 38-39 36.983662811788335 40.0 36.0 41.0 31.0 41.0 40-41 36.77749056993531 39.5 36.0 41.0 30.0 41.0 42-43 36.510753674618265 39.0 36.0 41.0 30.0 41.0 44-45 36.30924503221169 39.0 35.5 41.0 30.0 41.0 46-47 36.18263801651227 39.0 35.5 41.0 29.5 41.0 48-49 35.96011955944887 39.0 35.0 41.0 28.0 41.0 50-51 35.3369801708328 38.5 34.5 40.5 27.5 40.5 52-53 35.42423220127772 39.0 35.0 40.0 28.0 41.0 54-55 35.57008771723577 39.0 35.0 41.0 27.5 41.0 56-57 35.381362144714124 39.0 35.0 41.0 27.0 41.0 58-59 35.13768965094485 38.5 35.0 40.0 26.5 41.0 60-61 34.78550570506318 38.0 34.5 40.0 26.0 41.0 62-63 34.37876362866874 37.5 34.0 40.0 25.5 41.0 64-65 33.9534910133079 37.0 34.0 40.0 23.5 41.0 66-67 33.530826990642154 36.5 33.0 39.5 22.5 41.0 68-69 33.0599611041321 36.0 33.0 39.0 22.0 41.0 70-71 32.593139732901605 35.5 32.0 39.0 20.5 40.0 72-73 32.09880742850617 35.0 32.0 38.0 18.5 40.0 74-75 31.564730298470366 35.0 31.0 37.0 17.0 39.0 76-77 31.029271407842323 35.0 31.0 36.5 10.5 39.0 78-79 30.471116333241525 34.5 30.5 36.0 7.0 38.5 80-81 29.945155314760946 34.0 30.0 35.5 4.0 37.0 82-83 29.430672440027138 34.0 29.0 35.0 2.0 37.0 84-85 28.94647262678105 34.0 29.0 35.0 2.0 36.0 86-87 28.45187056207552 34.0 28.5 35.0 2.0 36.0 88-89 27.928469432483087 34.0 27.0 35.0 2.0 35.5 90-91 27.343251625699487 33.0 26.0 35.0 2.0 35.0 92-93 26.746688980847647 33.0 25.0 35.0 2.0 35.0 94-95 26.175237766947244 32.5 24.5 35.0 2.0 35.0 96-97 25.355261942509355 32.0 21.0 34.5 2.0 35.0 98-99 24.309508074755307 32.0 9.0 34.0 2.0 35.0 100-101 21.430029423817167 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3835738941462772 1101 2 0.3488399498193573 1101 3 0.34093229335247344 1101 4 0.24777348593304538 1101 5 0.21085585853840172 1101 6 0.16337875121109846 1101 7 0.26295537705586014 1101 8 0.21301892486970786 1101 9 0.2996241549836185 1101 10-11 0.27401258757844005 1101 12-13 0.26720435343619897 1101 14-15 0.3418576334665673 1101 16-17 0.5282376482990898 1101 18-19 0.3741713069137518 1101 20-21 0.4732985918081525 1101 22-23 0.3749078797932768 1101 24-25 0.4667766113916869 1101 26-27 0.4775741451786004 1101 28-29 0.47941330683953964 1101 30-31 0.46840022414000515 1101 32-33 0.5126870487878179 1101 34-35 0.5229708669471975 1101 36-37 0.4038807032588707 1101 38-39 0.4557079865775293 1101 40-41 0.5520087185118498 1101 42-43 0.4588697907686168 1101 44-45 0.4176535145068172 1101 46-47 0.5035304334204298 1101 48-49 0.5133949840180421 1101 50-51 0.4308151886440612 1101 52-53 0.5538892835295783 1101 54-55 0.3981903337345116 1101 56-57 0.38362603711293275 1101 58-59 0.27182878143707256 1101 60-61 0.31551540370552544 1101 62-63 0.3772412218633434 1101 64-65 0.39394977750567506 1101 66-67 0.19757004653407506 1101 68-69 0.32520454396238563 1101 70-71 0.5098294278220905 1101 72-73 0.5735933559366657 1101 74-75 0.5162726057727518 1101 76-77 0.5361047838167003 1101 78-79 0.7070541121487892 1101 80-81 0.7084177362360684 1101 82-83 0.5648371551107481 1101 84-85 0.5602317315436984 1101 86-87 0.5541075519837619 1101 88-89 0.6023843225958103 1101 90-91 0.7259538227399567 1101 92-93 0.8503584799292874 1101 94-95 0.98712112602934 1101 96-97 1.0690134144249974 1101 98-99 1.0552221376266573 1101 100-101 1.2257775980535293 1102 1 0.31955628233483324 1102 2 0.20105816762734463 1102 3 0.16487264089993658 1102 4 0.09148952460611781 1102 5 0.13292607311739602 1102 6 0.1583585031001391 1102 7 0.07115823885711592 1102 8 0.1394483934741615 1102 9 0.09625962028861323 1102 10-11 0.30305991164486556 1102 12-13 0.2056238374474404 1102 14-15 0.15758387955754216 1102 16-17 0.20171021163139358 1102 18-19 0.2294227553234407 1102 20-21 0.2714898931023626 1102 22-23 0.2956410478190392 1102 24-25 0.28804158636229715 1102 26-27 0.2579127009163571 1102 28-29 0.2238292551820038 1102 30-31 0.1627427210402388 1102 32-33 0.26752556289358154 1102 34-35 0.4012786669726722 1102 36-37 0.342669363686845 1102 38-39 0.44603272994037724 1102 40-41 0.48878142571567906 1102 42-43 0.4747794144921187 1102 44-45 0.47421880184536036 1102 46-47 0.49802999023157213 1102 48-49 0.5274060705305104 1102 50-51 0.5557667902161896 1102 52-53 0.5569281241168156 1102 54-55 0.5377068356546104 1102 56-57 0.6353505171410916 1102 58-59 0.7704543349802506 1102 60-61 0.6467887897176112 1102 62-63 0.6662951361864629 1102 64-65 0.5737497155793108 1102 66-67 0.5873080444621195 1102 68-69 0.5216682059207969 1102 70-71 0.45716642673965424 1102 72-73 0.5172416417317649 1102 74-75 0.5166177675145356 1102 76-77 0.3901491011277187 1102 78-79 0.3039609039122304 1102 80-81 0.3650006015292888 1102 82-83 0.5142735680899371 1102 84-85 0.5071693388592742 1102 86-87 0.550799628932289 1102 88-89 0.45985968737773675 1102 90-91 0.4849929365433354 1102 92-93 0.5377507952726752 1102 94-95 0.39494433944086893 1102 96-97 0.4834084798423497 1102 98-99 0.6673242149550909 1102 100-101 0.6673794201768324 1103 1 0.2731616048210377 1103 2 0.27437190748290874 1103 3 0.17121968306740598 1103 4 -0.006997213966158711 1103 5 -0.08643469674221649 1103 6 -0.06339157728214673 1103 7 0.1456611651496189 1103 8 0.14034339487437109 1103 9 0.2760896529978396 1103 10-11 0.13954621082538665 1103 12-13 0.030458711952071837 1103 14-15 0.06507720617742052 1103 16-17 0.049445694024136344 1103 18-19 0.15321214146676 1103 20-21 0.11005208259335575 1103 22-23 0.08354287676364436 1103 24-25 0.23795799821003527 1103 26-27 -0.0234765124875409 1103 28-29 0.07808725357340762 1103 30-31 0.22988753268166562 1103 32-33 0.08620197538248675 1103 34-35 0.04121532289969565 1103 36-37 0.11988935280576385 1103 38-39 0.12911388446578087 1103 40-41 0.07624678589905187 1103 42-43 0.12945568366011173 1103 44-45 0.08117608938388798 1103 46-47 0.153786540798869 1103 48-49 0.22663694638159626 1103 50-51 0.12736360208014474 1103 52-53 0.1507694735209455 1103 54-55 0.1647794965299738 1103 56-57 0.1400096726603124 1103 58-59 0.2955358723233701 1103 60-61 0.226392620534817 1103 62-63 0.1851170382624474 1103 64-65 0.18191947920352192 1103 66-67 0.16321193270198364 1103 68-69 0.29656818772281923 1103 70-71 0.3065094606359935 1103 72-73 0.2707400392719279 1103 74-75 0.15536844286720708 1103 76-77 0.2772232517862854 1103 78-79 0.18568343273434706 1103 80-81 0.20996414427144927 1103 82-83 0.2741162733853564 1103 84-85 0.2643700362286765 1103 86-87 0.45716581198515627 1103 88-89 0.49820246386217093 1103 90-91 0.49992065848567435 1103 92-93 0.5933066965585105 1103 94-95 0.5570835738243076 1103 96-97 0.5190189185080705 1103 98-99 0.7513452529568205 1103 100-101 0.9450623735683017 1104 1 0.37083380325072923 1104 2 0.36398914517027237 1104 3 0.32456550747853896 1104 4 0.1682624220081479 1104 5 0.27572715193730346 1104 6 0.23857041415254088 1104 7 0.25039260697688803 1104 8 0.3462686268515256 1104 9 0.4014126365809929 1104 10-11 0.36444806664266594 1104 12-13 0.3342087119520656 1104 14-15 0.35357738462138855 1104 16-17 0.5258094520541121 1104 18-19 0.4388219737294321 1104 20-21 0.4179906086461713 1104 22-23 0.3967468382197481 1104 24-25 0.5381690082028854 1104 26-27 0.5098322848001118 1104 28-29 0.45558591524364545 1104 30-31 0.4219583749371978 1104 32-33 0.4382977997936308 1104 34-35 0.5911550980602982 1104 36-37 0.6702007375309691 1104 38-39 0.5191758937448654 1104 40-41 0.5809420928226885 1104 42-43 0.6595047557514846 1104 44-45 0.49096775605055853 1104 46-47 0.3806062231685985 1104 48-49 0.4963242233266314 1104 50-51 0.5457442230651637 1104 52-53 0.5695252729499245 1104 54-55 0.5528317806127703 1104 56-57 0.6229410609543891 1104 58-59 0.6451218823162321 1104 60-61 0.6483764713556539 1104 62-63 0.6289379697399653 1104 64-65 0.6194226019729712 1104 66-67 0.622863966963223 1104 68-69 0.6186180628120397 1104 70-71 0.665645345718076 1104 72-73 0.648993072819394 1104 74-75 0.6361411052512231 1104 76-77 0.7229325665614468 1104 78-79 0.6407646247400578 1104 80-81 0.7065090229295556 1104 82-83 0.7746806024360389 1104 84-85 0.6955593652793546 1104 86-87 0.7440033387517531 1104 88-89 0.6682510898436149 1104 90-91 0.7907673751166513 1104 92-93 0.6567564645813526 1104 94-95 0.8760138022325847 1104 96-97 0.933778911370311 1104 98-99 0.9910084399660981 1104 100-101 0.8940934228188375 1105 1 0.45177829315798945 1105 2 0.35737498805080037 1105 3 0.3398478315374547 1105 4 0.31183648092795124 1105 5 0.30631703191547643 1105 6 0.2721444730723448 1105 7 0.2108679206702888 1105 8 0.2583846934090843 1105 9 0.3176387686050006 1105 10-11 0.3796621964844462 1105 12-13 0.2535356350289888 1105 14-15 0.2877270027337673 1105 16-17 0.3694806877333292 1105 18-19 0.35825766658267355 1105 20-21 0.31437801999369697 1105 22-23 0.26284953871074634 1105 24-25 0.39583096946857665 1105 26-27 0.3058548570859827 1105 28-29 0.5269330920030555 1105 30-31 0.52152943331145 1105 32-33 0.5250019732797142 1105 34-35 0.42540734846946293 1105 36-37 0.4968121668817531 1105 38-39 0.5051350454088137 1105 40-41 0.5509622101167366 1105 42-43 0.5103592292921206 1105 44-45 0.4821171695802917 1105 46-47 0.44090116315769023 1105 48-49 0.5272833749905743 1105 50-51 0.39081173534011526 1105 52-53 0.2807299237955334 1105 54-55 0.30800294809777284 1105 56-57 0.4796285268572831 1105 58-59 0.49154304980122987 1105 60-61 0.4168274397135363 1105 62-63 0.30700773242409696 1105 64-65 0.4067892822784813 1105 66-67 0.5101021011694371 1105 68-69 0.4650545739958929 1105 70-71 0.5808229916537044 1105 72-73 0.5723986920228867 1105 74-75 0.5749367953766971 1105 76-77 0.4615966418478621 1105 78-79 0.6009971670204699 1105 80-81 0.7163920698471742 1105 82-83 0.773471859937402 1105 84-85 0.717285360914925 1105 86-87 0.8056594353147659 1105 88-89 0.74017791744863 1105 90-91 0.7960776588045952 1105 92-93 0.7938824193003917 1105 94-95 0.8328052643166046 1105 96-97 0.8155580984952415 1105 98-99 0.9100629271455851 1105 100-101 0.9220107714276757 1106 1 0.0212312469307534 1106 2 0.0828210957975557 1106 3 -0.02475157062749389 1106 4 0.033053498495398514 1106 5 0.06270222950374205 1106 6 0.1192214663581268 1106 7 0.1876145481612923 1106 8 0.0013588399906083737 1106 9 0.13721359435787406 1106 10-11 -0.42676163253445765 1106 12-13 -0.2537177348306088 1106 14-15 -0.14710435691375068 1106 16-17 0.0473981307267195 1106 18-19 0.041781386247976116 1106 20-21 0.03545528219265037 1106 22-23 -0.0550383701978987 1106 24-25 -0.06718552100255692 1106 26-27 -0.029450478588522344 1106 28-29 -0.13261496496679825 1106 30-31 -0.05244959079866618 1106 32-33 -0.023488420360159523 1106 34-35 -0.04677868380938577 1106 36-37 -0.302579682002289 1106 38-39 0.04069897819110935 1106 40-41 0.253643344003045 1106 42-43 0.04419600760633102 1106 44-45 0.29287175915320773 1106 46-47 0.3889541423646108 1106 48-49 0.32448227337385305 1106 50-51 0.29241004661837877 1106 52-53 0.2873251684038607 1106 54-55 -0.11947312293504098 1106 56-57 -0.13162245921289184 1106 58-59 0.008927359181200245 1106 60-61 -0.11459963480918844 1106 62-63 0.08207966338626704 1106 64-65 0.09618040920350523 1106 66-67 -0.1149941876300602 1106 68-69 -0.4528974336131597 1106 70-71 -0.1537249328466075 1106 72-73 0.14439001063134782 1106 74-75 0.2701877125626986 1106 76-77 0.27372121488196655 1106 78-79 0.5520159737215771 1106 80-81 0.4498218686060689 1106 82-83 0.4099796547765209 1106 84-85 0.3330541379759673 1106 86-87 0.22937742818922757 1106 88-89 0.11798188371923501 1106 90-91 0.034462066874372255 1106 92-93 0.28583232792952273 1106 94-95 0.325052484277645 1106 96-97 0.4992552704691917 1106 98-99 0.5144624228340327 1106 100-101 0.32231377894995816 1107 1 0.25008223646655736 1107 2 0.2531102771718423 1107 3 0.17484459090979243 1107 4 0.04606157986165016 1107 5 0.09678913783624665 1107 6 0.10625206319288338 1107 7 0.03196379773286395 1107 8 0.16344792571560873 1107 9 0.20483703924453067 1107 10-11 0.1668423087108195 1107 12-13 0.12501413694227637 1107 14-15 0.16038163452346765 1107 16-17 0.12908966161150204 1107 18-19 0.27904940224490105 1107 20-21 0.30423350915538094 1107 22-23 0.36867838933607544 1107 24-25 0.38648251192400096 1107 26-27 0.41219592561484575 1107 28-29 0.46839608954838496 1107 30-31 0.4149817787758181 1107 32-33 0.3863799580388303 1107 34-35 0.4648074188593583 1107 36-37 0.41303416878830745 1107 38-39 0.4902542819156466 1107 40-41 0.4001437673232715 1107 42-43 0.4486317631937027 1107 44-45 0.4659391132773507 1107 46-47 0.5276276195649956 1107 48-49 0.4804793839220096 1107 50-51 0.41633127751091337 1107 52-53 0.5341406763968521 1107 54-55 0.6017462929511979 1107 56-57 0.5448498447381667 1107 58-59 0.43579559951168534 1107 60-61 0.5108992386882036 1107 62-63 0.4929124605351092 1107 64-65 0.4205264503865962 1107 66-67 0.3843631835711392 1107 68-69 0.34222484894599603 1107 70-71 0.3728410468539991 1107 72-73 0.5257370994547301 1107 74-75 0.5256583398771504 1107 76-77 0.5636381090604701 1107 78-79 0.41837185153206846 1107 80-81 0.46100985528364546 1107 82-83 0.5545119283271482 1107 84-85 0.5467241713898154 1107 86-87 0.5717121617051433 1107 88-89 0.5459016480104779 1107 90-91 0.7716070735106975 1107 92-93 0.814777175509672 1107 94-95 0.6711562816685444 1107 96-97 0.7616681593139063 1107 98-99 0.8715222962136515 1107 100-101 1.0021809668846444 1108 1 0.2972546581902371 1108 2 0.20128421896365722 1108 3 0.2405956231546469 1108 4 0.17221286077958808 1108 5 0.20250035363920205 1108 6 0.24859584295190018 1108 7 0.22559819798872383 1108 8 0.22743452899219818 1108 9 0.29944780477671173 1108 10-11 0.30364664395951024 1108 12-13 0.09611487469639002 1108 14-15 0.25458273630334816 1108 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66-67 -1.3676160552343362 2316 68-69 -1.192948021168725 2316 70-71 -1.310006013882365 2316 72-73 -1.19730640923758 2316 74-75 -1.2210314807643208 2316 76-77 -1.1782850619994107 2316 78-79 -1.147463732640631 2316 80-81 -1.1475108161381478 2316 82-83 -1.3875222739975541 2316 84-85 -1.302558929208253 2316 86-87 -1.253782686035521 2316 88-89 -1.3333496778219427 2316 90-91 -1.333669824291416 2316 92-93 -1.4125339072277505 2316 94-95 -1.5955691611414409 2316 96-97 -1.5241238818224367 2316 98-99 -1.614991143829684 2316 100-101 -1.5059907429229291 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6327.0 3 1694.0 4 1735.0 5 2037.0 6 2268.0 7 2747.0 8 3295.0 9 4097.0 10 5133.0 11 6286.0 12 7768.0 13 9802.0 14 11115.0 15 13204.0 16 16554.0 17 22150.0 18 19920.0 19 15593.0 20 15527.0 21 16531.0 22 17967.0 23 19408.0 24 21971.0 25 24823.0 26 28288.0 27 32586.0 28 38055.0 29 45761.0 30 55066.0 31 67370.0 32 84585.0 33 109842.0 34 151140.0 35 222654.0 36 341886.0 37 494871.0 38 396826.0 39 44862.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.80576528558493 18.555277728316465 14.177009238060437 23.461947748038167 2 15.59295320803444 19.581399822985876 40.30171372691182 24.523933242067862 3 19.426124794310905 23.599938330029286 29.298529288825094 27.675407586834723 4 12.92745936575838 16.159166657213667 36.43328682457796 34.48008715244998 5 14.253509645484838 36.77717690462084 33.80814918461933 15.161164265274993 6 32.87253399148201 35.32075290885714 17.18170695263406 14.625006147026792 7 29.453824922974665 32.18846071592283 20.00431921702847 18.35339514407404 8 26.562779619150838 35.06168949829235 19.3240830592278 19.051447823329006 9 26.556852570963844 16.779233676173323 20.023289146686338 36.640624606176495 10-11 25.510518570184555 26.370281822259432 26.696884293101874 21.422315314454142 12-13 25.962511456280563 23.606148153114123 27.52506301085659 22.906277379748722 14-15 22.881275473467266 25.06841235446884 25.690929072120948 26.359383099942953 16-17 22.64305195427177 28.468183322942593 26.226576718127447 22.66218800465819 18-19 22.498856256309494 26.766059988883946 28.69143538677862 22.04364836802794 20-21 23.06881870515507 26.039209128376513 28.87735204140156 22.01462012506686 22-23 22.914171740543786 25.8537820121647 28.73123488208684 22.50081136520467 24-25 22.587719943336136 26.15450947136277 28.79162329538793 22.46614728991317 26-27 22.397340559616836 26.518695907906896 28.614864297117887 22.46909923535837 28-29 22.244729648996177 26.512161572841297 28.915802104191158 22.32730667397137 30-31 22.430670863696253 26.206884092070236 28.82476169028476 22.537683353948747 32-33 22.292674769725 26.277630794478696 28.735017892188065 22.694676543608246 34-35 22.80156139019272 26.37613739326155 28.45955256982129 22.36274864672444 36-37 22.693118317563076 26.116198173531135 28.456307573440764 22.734375935465017 38-39 22.36817732579019 26.396372973206827 28.608136028625758 22.627313672377227 40-41 22.674088798860005 26.23078966740069 28.55765121229209 22.537470321447213 42-43 22.57664467508051 26.17266174358681 28.46758113693994 22.783112444392735 44-45 22.551350997223864 26.461651139736908 28.311989973061735 22.67500788997749 46-47 22.559205380064153 26.745806668381622 27.867178353746567 22.827809597807654 48-49 22.787995391888934 26.550880106374624 27.935098375947444 22.726026125789 50-51 22.631052874721767 26.778652723531142 27.746797698542693 22.8434967032044 52-53 22.79706717292798 26.803004726146213 27.64828081628128 22.75164728464453 54-55 22.62069249357659 26.699820337704637 27.77214554073815 22.907341627980617 56-57 22.607424971452595 26.987913706408772 27.76634761606584 22.638313706072797 58-59 22.798494723089775 26.53963950457391 27.789081492997507 22.87278427933881 60-61 22.758852694861766 26.595347706181393 27.95889024147216 22.68690935748468 62-63 22.60957078773046 26.67513142279294 27.86578959082766 22.849508198648945 64-65 22.48867896557473 26.515935119753298 27.907279016725695 23.088106897946275 66-67 22.444080971047875 26.773839993785025 27.75773774904518 23.024341286121917 68-69 22.388339554334568 26.546167444003117 27.943735300929255 23.121757700733063 70-71 22.34399801410206 26.661120881456423 27.863811420341257 23.131069684100257 72-73 22.678586616517194 26.593500519240237 27.63011194378555 23.097800920457015 74-75 22.481780825599916 26.80658779810904 27.592893430009475 23.118737946281577 76-77 22.595613154656665 26.723997463474454 27.56774916953986 23.112640212329026 78-79 22.61361450812189 26.761590733292408 27.571911132462283 23.05288362612342 80-81 22.430084917919814 27.009223325331398 27.59766253677876 22.96302921997002 82-83 22.690920452724832 26.960292611042043 27.38102912356744 22.96775781266569 84-85 22.76078615159719 26.694252775093634 27.330551625581467 23.214409447727704 86-87 22.65160323472861 26.554683986632117 27.62796505942191 23.16574771921736 88-89 22.68005664517026 26.678841920030177 27.464527988766736 23.176573446032833 90-91 22.76953490184267 26.736050069301488 27.54702135922493 22.94739366963091 92-93 22.815182794082165 26.408526939528677 27.52859494538259 23.247695321006574 94-95 22.75496555762702 26.434888834185116 27.52728630731889 23.282859300868978 96-97 22.9322998770555 26.38686396591997 27.439053843979355 23.24178231304518 98-99 22.89257298663074 26.293267825403277 27.185062982363593 23.62909620560239 100-101 23.17046602531373 25.986464968822574 27.432621351383407 23.410447654480286 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 902.0 1 615.0 2 480.0 3 656.0 4 531.5 5 488.5 6 990.5 7 2090.5 8 3471.5 9 4122.0 10 3479.0 11 2705.5 12 2631.5 13 2749.5 14 2845.5 15 2942.5 16 3012.0 17 3084.5 18 3208.0 19 3412.5 20 3711.5 21 4239.5 22 5116.0 23 6200.0 24 7286.5 25 8895.5 26 11419.5 27 14347.0 28 16610.0 29 19421.0 30 23326.5 31 26765.5 32 30842.0 33 35116.5 34 39024.0 35 42867.0 36 47269.5 37 52133.0 38 56573.0 39 59537.5 40 62437.5 41 68980.5 42 75371.0 43 80640.5 44 86551.5 45 90776.0 46 92137.0 47 93855.5 48 97525.0 49 98249.0 50 96291.0 51 92920.5 52 88697.5 53 86340.5 54 85236.5 55 79941.5 56 71347.0 57 65295.5 58 59821.0 59 52558.0 60 43960.0 61 35478.5 62 28310.5 63 22083.0 64 17545.0 65 14010.0 66 10447.0 67 8302.5 68 6253.0 69 4003.5 70 2856.5 71 2175.5 72 1805.0 73 1464.5 74 1071.0 75 859.0 76 599.5 77 341.0 78 203.5 79 118.5 80 61.0 81 42.0 82 34.5 83 22.5 84 9.0 85 7.0 86 7.0 87 5.0 88 5.5 89 6.5 90 4.0 91 1.5 92 2.5 93 2.0 94 1.0 95 0.5 96 1.0 97 1.5 98 0.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4940077523025144 2 0.047990044270081085 3 0.05588341988055811 4 0.06495240462451043 5 0.07868183986188272 6 0.10685447302480872 7 0.07066250612996192 8 0.05349021557312625 9 0.05189474603483834 10-11 0.0750080613197724 12-13 0.10876483786670607 14-15 0.05567348967815181 16-17 0.05962017748339032 18-19 0.05890641479520889 20-21 0.07292975231594999 22-23 0.06283210958020677 24-25 0.08804472688920388 26-27 0.06482644650306665 28-29 0.06474247442210414 30-31 0.06919299471311778 32-33 0.06948689699648661 34-35 0.07076747123116506 36-37 0.0662329788591889 38-39 0.039928724497679015 40-41 0.0430986705540142 42-43 0.04135624987404188 44-45 0.023449203608784153 46-47 0.03071278861204227 48-49 0.03043987934891407 50-51 0.02703901006993195 52-53 0.02638822644247241 54-55 0.025611484693569084 56-57 0.025905386976937905 58-59 0.02187472709073687 60-61 0.029537179478566968 62-63 0.020909048159667872 64-65 0.02338622454806226 66-67 0.016752430152023056 68-69 0.017991018346220248 70-71 0.04018064074056658 72-73 0.036170973874606174 74-75 0.03090172579420794 76-77 0.022420545616993262 78-79 0.01687838827346684 80-81 0.01866279499392042 82-83 0.023617147770709196 84-85 0.019859397147636355 86-87 0.009026998703471071 88-89 0.04024361980128847 90-91 0.050530199719197366 92-93 0.03243421627177396 94-95 0.05231460643965095 96-97 0.041776110278854486 98-99 0.0319093907657582 100-101 0.1705472964348813 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2381744.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.102309393061 #Duplication Level Percentage of deduplicated Percentage of total 1 76.76005180371496 40.76136019908262 2 12.959110070292882 13.7631734482285 3 4.229141064355397 6.737314717988986 4 1.9293631780168223 4.098145616425149 5 1.0088799389429675 2.6786927329100974 6 0.6306316907732158 2.0092799493905082 7 0.43257296432738007 1.6079436370750253 8 0.3219241880214614 1.3675934266740468 9 0.21862177506682423 1.044838902569282 >10 1.3224163430701437 13.090592324926353 >50 0.11823341203921782 4.36110437524042 >100 0.06556091982314725 6.169831974039827 >500 0.002540110222365435 0.8351533590538203 >1k 7.937844444888979E-4 0.7237040337955123 >5k 1.5875688889777957E-4 0.7512713025998844 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9953 0.4178870609099886 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7870 0.3304301385875224 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4941 0.20745302601790955 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 4.1986040481260794E-5 0.0 0.0 0.0 0.0 8 4.1986040481260794E-5 0.0 0.0 0.0 0.0 9 4.1986040481260794E-5 0.0 0.0 0.0 0.0 10-11 4.1986040481260794E-5 0.0 0.0 0.0 0.0 12-13 4.1986040481260794E-5 0.0 0.0 0.0 0.0 14-15 4.1986040481260794E-5 0.0 0.0 0.0 0.0 16-17 4.1986040481260794E-5 0.0 0.0 4.1986040481260794E-5 0.0 18-19 4.1986040481260794E-5 0.0 0.0 4.1986040481260794E-5 0.0 20-21 4.1986040481260794E-5 0.0 0.0 6.297906072189119E-5 0.0 22-23 4.1986040481260794E-5 0.0 0.0 1.8893718216567357E-4 0.0 24-25 8.397208096252159E-5 0.0 0.0 6.087975869782814E-4 0.0 26-27 8.397208096252159E-5 0.0 0.0 0.001385539335881606 0.0 28-29 1.0496510120315197E-4 0.0 0.0 0.0034428553194633844 0.0 30-31 1.2595812144378237E-4 0.0 0.0 0.012322902881250042 0.0 32-33 1.8893718216567357E-4 0.0 0.0 0.030502858409635965 0.0 34-35 2.729092631281951E-4 0.0 0.0 0.05943124030122465 0.0 36-37 2.9390228336882555E-4 0.0 0.0 0.11086413989076913 0.0 38-39 2.9390228336882555E-4 0.0 0.0 0.18398282938888477 0.0 40-41 3.3588832385008635E-4 0.0 0.0 0.26255970414956437 0.0 42-43 3.568813440907167E-4 0.0 0.0 0.33954110937195603 0.0 44-45 3.778743643313471E-4 0.0 0.0 0.4330230285034832 0.0 46-47 5.038324857751295E-4 0.0 0.0 0.5479598143209345 0.0 48-49 5.458185262563902E-4 0.0 0.0 0.6629175931586266 0.0 50-51 5.458185262563902E-4 0.0 0.0 0.7847610826352455 0.0 52-53 5.458185262563902E-4 0.0 0.0 0.8984802732787402 0.0 54-55 5.458185262563902E-4 0.0 0.0 1.0248582551273353 0.0 56-57 5.878045667376511E-4 0.0 0.0 1.1726701106416137 0.0 58-59 5.878045667376511E-4 0.0 0.0 1.3337075689074895 0.0 60-61 5.878045667376511E-4 0.0 0.0 1.4972431965820006 0.0 62-63 5.878045667376511E-4 0.0 0.0 1.6564542620869414 0.0 64-65 6.087975869782814E-4 0.0 0.0 1.8251331797204067 0.0 66-67 6.297906072189118E-4 0.0 0.0 2.0144062502099302 0.0 68-69 6.297906072189118E-4 0.0 0.0 2.218962239434633 0.0 70-71 6.297906072189118E-4 0.0 0.0 2.435526236236976 0.0 72-73 6.297906072189118E-4 0.0 0.0 2.6506627076629563 0.0 74-75 6.297906072189118E-4 0.0 0.0 2.86325902363982 0.0 76-77 6.297906072189118E-4 0.0 0.0 3.0977930457681433 4.1986040481260794E-5 78-79 6.297906072189118E-4 0.0 0.0 3.350276100202205 4.1986040481260794E-5 80-81 6.717766477001727E-4 0.0 0.0 3.61986846613238 4.1986040481260794E-5 82-83 6.717766477001727E-4 0.0 0.0 3.894625115041751 4.1986040481260794E-5 84-85 7.137626881814334E-4 0.0 0.0 4.171292128793018 4.1986040481260794E-5 86-87 7.557487286626942E-4 0.0 0.0 4.460428996567222 4.1986040481260794E-5 88-89 7.557487286626942E-4 0.0 0.0 4.77574416058149 4.1986040481260794E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4130 0.0 58.840942 1 GTATCAA 7465 0.0 46.240276 1 ATCAACG 9670 0.0 35.038338 3 TATCAAC 9805 0.0 34.749046 2 TCAACGC 9815 0.0 34.61754 4 CAACGCA 9845 0.0 34.512054 5 AACGCAG 10065 0.0 33.757694 6 ACGCAGA 11825 0.0 28.749002 7 CGCAGAG 11960 0.0 28.265476 8 GTACATG 8730 0.0 25.594315 1 GCAGAGT 13175 0.0 25.334036 9 TACATGG 8710 0.0 25.041819 2 GTGGTAT 1895 0.0 23.934607 1 GAGTACT 7740 0.0 23.799923 12-13 TGGTATC 1740 0.0 23.213522 2 ACATGGG 9045 0.0 23.064095 3 CAGAGTA 12715 0.0 21.986265 10-11 GTACTTT 8430 0.0 21.723345 14-15 AGTACTT 8140 0.0 21.462374 12-13 CATGGGG 5335 0.0 20.486788 4 >>END_MODULE