FastQCFastQC Report
Thu 26 May 2016
SRR938153_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938153_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2381744
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95350.4003368959888216No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81120.34059076038398756No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50390.21156765798507313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39650.056.080261
GTATCAA71800.043.9390871
ATCAACG90850.034.246353
TCAACGC91550.033.9290544
TATCAAC92300.033.81982
CAACGCA93300.033.2926565
AACGCAG96500.032.2870866
TGGTATC17050.027.587872
ACGCAGA113100.027.4642337
CGCAGAG114900.026.951318
GTACATG88050.026.7645321
TACATGG88800.025.7359072
GTGGTAT21300.025.4291951
ACATGGG90800.024.7441863
GCAGAGT129400.023.527529
GAGTACT75250.021.93313212-13
CATGGGG50600.021.682724
CAGAGTA125050.020.5858110-11
GTACTTT82200.020.04979714-15
AGGACCG8200.019.693255
Produced by FastQC (version 0.11.5)