##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938153_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2381744 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.523688943899934 34.0 31.0 34.0 31.0 34.0 2 32.73984105764515 34.0 31.0 34.0 31.0 34.0 3 32.801300223701624 34.0 31.0 34.0 31.0 34.0 4 36.24947265533156 37.0 35.0 37.0 35.0 37.0 5 36.21232382657414 37.0 37.0 37.0 35.0 37.0 6 36.24874545711042 37.0 37.0 37.0 35.0 37.0 7 36.215922869964196 37.0 37.0 37.0 35.0 37.0 8 36.21180403939298 37.0 37.0 37.0 35.0 37.0 9 37.92461532389711 39.0 38.0 39.0 35.0 39.0 10-11 37.911460467623726 39.0 38.0 39.0 35.0 39.0 12-13 37.85700268374771 39.0 38.0 39.0 35.0 39.0 14-15 39.201905830349524 40.5 39.0 41.0 36.0 41.0 16-17 39.155741129189366 40.0 39.0 41.0 36.0 41.0 18-19 39.172736238655375 40.0 39.0 41.0 36.0 41.0 20-21 39.099141007597794 40.0 39.0 41.0 36.0 41.0 22-23 38.955718792615826 40.0 38.0 41.0 35.0 41.0 24-25 38.92353859188897 40.0 38.0 41.0 35.0 41.0 26-27 38.905663455014476 40.0 38.0 41.0 35.0 41.0 28-29 38.85040709664851 40.0 38.0 41.0 35.0 41.0 30-31 38.68373553161045 40.0 38.0 41.0 35.0 41.0 32-33 38.540605119609836 40.0 38.0 41.0 34.5 41.0 34-35 38.352685469135224 40.0 38.0 41.0 34.0 41.0 36-37 38.2417734651583 40.0 38.0 41.0 34.0 41.0 38-39 38.25876647532228 40.0 38.0 41.0 34.0 41.0 40-41 38.217204493849884 40.0 38.0 41.0 34.0 41.0 42-43 38.08166851685152 40.0 38.0 41.0 33.5 41.0 44-45 37.89563718854755 40.0 38.0 41.0 33.0 41.0 46-47 37.59602417388267 40.0 37.0 41.0 33.0 41.0 48-49 37.29595645039937 40.0 37.0 41.0 32.0 41.0 50-51 37.02917672932104 40.0 37.0 41.0 31.5 41.0 52-53 36.639835557473845 39.0 36.0 41.0 31.0 41.0 54-55 36.43053493574456 39.0 35.5 41.0 30.0 41.0 56-57 36.207223152446275 39.0 35.0 41.0 30.0 41.0 58-59 35.89179378640189 38.5 35.0 40.0 29.5 41.0 60-61 35.55014098912393 38.0 35.0 40.0 28.5 41.0 62-63 35.198677103836516 38.0 34.5 40.0 28.0 41.0 64-65 34.81313776795491 37.0 34.0 40.0 28.0 41.0 66-67 34.399148061252596 36.5 34.0 39.5 26.5 41.0 68-69 33.950629034858494 36.0 33.5 39.0 26.0 40.5 70-71 33.45039559247341 35.5 33.0 38.5 26.0 40.0 72-73 33.026347290053 35.0 33.0 38.0 25.5 40.0 74-75 32.57272842925184 35.0 32.0 37.0 24.5 39.0 76-77 31.248745667040623 34.0 30.5 36.0 23.0 38.5 78-79 31.972202512108773 35.0 32.0 36.0 24.5 39.0 80-81 31.80416745040609 35.0 32.0 36.0 24.0 37.5 82-83 31.444135473837658 35.0 32.0 35.5 23.0 37.0 84-85 31.071774926272514 34.5 31.0 35.0 21.0 36.5 86-87 30.639349149194878 34.0 31.0 35.0 19.5 36.0 88-89 30.201255886442876 34.0 31.0 35.0 18.0 36.0 90-91 29.875567651267303 34.0 30.0 35.0 13.5 35.0 92-93 29.47423316695665 34.0 30.0 35.0 7.0 35.0 94-95 29.07546256020798 34.0 29.5 35.0 2.0 35.0 96-97 28.508078114188592 34.0 29.0 35.0 2.0 35.0 98-99 27.83241187969824 34.0 29.0 35.0 2.0 35.0 100-101 25.381926437098194 31.0 22.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15451275710375256 1101 2 0.11929839054637625 1101 3 0.1147514280585824 1101 4 0.05513333821149047 1101 5 0.06335095926475276 1101 6 0.05207641078623482 1101 7 0.05731032492849408 1101 8 0.058220245734744935 1101 9 -0.02020410718278498 1101 10-11 0.06178697816299916 1101 12-13 0.11912959777421861 1101 14-15 0.17200328718340785 1101 16-17 0.1138395519401314 1101 18-19 0.14351731105645626 1101 20-21 0.10033458609908052 1101 22-23 0.13420478457741325 1101 24-25 0.19843257979803042 1101 26-27 0.26399055807659266 1101 28-29 0.16765392624460418 1101 30-31 0.08490712030744163 1101 32-33 0.06964726020202505 1101 34-35 -0.042284081679156316 1101 36-37 0.04029056654596275 1101 38-39 0.1751130086122643 1101 40-41 0.1847361170052153 1101 42-43 0.2654732839702376 1101 44-45 0.23066846603740743 1101 46-47 0.17300690174806732 1101 48-49 0.16158268204489445 1101 50-51 0.16240374634542576 1101 52-53 0.12250326227797359 1101 54-55 0.2111485529189565 1101 56-57 0.1966923347655367 1101 58-59 0.16912926803573924 1101 60-61 0.2201715835274598 1101 62-63 0.18780140183488925 1101 64-65 0.24006069924060114 1101 66-67 0.2794750060948985 1101 68-69 0.25735963229679015 1101 70-71 0.18883440395143225 1101 72-73 0.170861952846586 1101 74-75 0.2485740299867487 1101 76-77 0.17444720348709808 1101 78-79 0.15210670046895203 1101 80-81 0.21161953662352673 1101 82-83 0.18513341271211914 1101 84-85 0.4638137047790458 1101 86-87 0.40786166037805316 1101 88-89 0.3249478821373195 1101 90-91 0.33269965569900606 1101 92-93 0.364812434839358 1101 94-95 0.30406396311184736 1101 96-97 0.4522130986363848 1101 98-99 0.5175705066824605 1101 100-101 0.6433576393279274 1102 1 0.15001079291987196 1102 2 0.09271119276957762 1102 3 0.08862188699673368 1102 4 0.00912397681878474 1102 5 0.0811446523418553 1102 6 0.013999765785925433 1102 7 0.026720746943105667 1102 8 0.03919314320566514 1102 9 -0.003494027101723418 1102 10-11 0.07156203349024537 1102 12-13 -0.02018543677049678 1102 14-15 0.0566645434619133 1102 16-17 0.05038815498484439 1102 18-19 0.026889746973196793 1102 20-21 0.13013384506919579 1102 22-23 0.1311209656850636 1102 24-25 0.027119467214667736 1102 26-27 -0.09763954231065242 1102 28-29 -0.012057531530807353 1102 30-31 0.03233358373122286 1102 32-33 -0.022685326511123094 1102 34-35 -0.04504163562310026 1102 36-37 -0.006595428902663514 1102 38-39 0.0182662771571529 1102 40-41 0.001784548108787476 1102 42-43 -0.0972190720996764 1102 44-45 -0.051536049626953684 1102 46-47 -0.0466267878488793 1102 48-49 -0.11130364764076717 1102 50-51 -0.09373411914759089 1102 52-53 -0.28170357729828766 1102 54-55 -0.1881588992874157 1102 56-57 -0.11417379385431303 1102 58-59 -0.20668710788604727 1102 60-61 -0.16872058605472517 1102 62-63 -0.220331148147622 1102 64-65 -0.2053245168137252 1102 66-67 -0.3165904846794305 1102 68-69 -0.04585457446995633 1102 70-71 -0.003019227785884482 1102 72-73 -0.1272181329448614 1102 74-75 0.033688258150178285 1102 76-77 0.06300513603617475 1102 78-79 -0.010613882686090648 1102 80-81 0.035507032201969935 1102 82-83 -0.04366079274366186 1102 84-85 -0.030464700496938946 1102 86-87 -0.057048864289445333 1102 88-89 -0.26658424821827253 1102 90-91 -0.2039160309622403 1102 92-93 -0.08702160722214813 1102 94-95 -0.33257884832157814 1102 96-97 -0.24074983392548788 1102 98-99 -0.27392886923125204 1102 100-101 -0.09873547973938912 1103 1 0.09629449101436194 1103 2 0.12799981940634098 1103 3 0.13987627502114464 1103 4 0.05460743503932264 1103 5 0.03395341226706705 1103 6 -0.0015356556954273515 1103 7 0.04776308601586976 1103 8 -0.01396307564914423 1103 9 -0.009712080445389404 1103 10-11 0.01018749162215471 1103 12-13 0.049653902369215075 1103 14-15 0.11470941988062577 1103 16-17 0.04803005447728026 1103 18-19 0.06525307869416963 1103 20-21 0.0563877852157475 1103 22-23 0.22088662655949776 1103 24-25 0.13949437964276257 1103 26-27 0.11086457392260485 1103 28-29 0.15063386553696034 1103 30-31 0.13265039838616133 1103 32-33 0.1732578429743441 1103 34-35 0.157517962776609 1103 36-37 0.13182787130656237 1103 38-39 0.16170458363195905 1103 40-41 0.23556953090555766 1103 42-43 0.1568691098650845 1103 44-45 0.23234534477240487 1103 46-47 0.3502177004718874 1103 48-49 0.2736744346310971 1103 50-51 0.3334009617580094 1103 52-53 0.3067649350023274 1103 54-55 0.3259858378648559 1103 56-57 0.3980937221773573 1103 58-59 0.2870793751978411 1103 60-61 0.21144172488188673 1103 62-63 0.39787617121380947 1103 64-65 0.3903309736502507 1103 66-67 0.4157875958952104 1103 68-69 0.4336318652027842 1103 70-71 0.19421845015126848 1103 72-73 0.15953675151679647 1103 74-75 0.20011014777986702 1103 76-77 0.22777478066630863 1103 78-79 0.2439599874595011 1103 80-81 0.3413053061278397 1103 82-83 0.3542229607933933 1103 84-85 0.35598383550819435 1103 86-87 0.39753848933699487 1103 88-89 0.23677294993326115 1103 90-91 0.18152048965852785 1103 92-93 0.16872626865816898 1103 94-95 0.17266264270624987 1103 96-97 0.32172507312806786 1103 98-99 0.37990180653629224 1103 100-101 0.4265827256941712 1104 1 0.14645509058609463 1104 2 0.08769468022004645 1104 3 0.08298120007467702 1104 4 0.05501785616708332 1104 5 0.04377675273816095 1104 6 0.10282462267716141 1104 7 0.036729003217871536 1104 8 0.016071720924728083 1104 9 -0.0201474837287563 1104 10-11 0.09464189918818278 1104 12-13 0.09598420215507986 1104 14-15 0.20970808155086473 1104 16-17 0.06820849844586974 1104 18-19 0.15873942773057337 1104 20-21 0.13820077593665303 1104 22-23 0.184706665817167 1104 24-25 0.14100624616667545 1104 26-27 0.08669032338727334 1104 28-29 0.10436557503017951 1104 30-31 0.1411840350742395 1104 32-33 0.08378291435193574 1104 34-35 0.13646871224128176 1104 36-37 0.1511921646684513 1104 38-39 0.1664190732822064 1104 40-41 0.14263769650155922 1104 42-43 0.16073777510419518 1104 44-45 0.006324199162129673 1104 46-47 0.0405380073955115 1104 48-49 -0.10936892725327141 1104 50-51 -0.053831818399018516 1104 52-53 0.03903563450268166 1104 54-55 -0.02928450474755806 1104 56-57 0.14048219469698608 1104 58-59 0.03442859004437793 1104 60-61 0.010638727023213335 1104 62-63 0.11030568584621392 1104 64-65 0.12491876808279301 1104 66-67 0.09387021545267515 1104 68-69 0.23342933129843146 1104 70-71 0.13099084486933066 1104 72-73 0.1316254381691877 1104 74-75 0.014549387608568054 1104 76-77 0.08181430600535222 1104 78-79 0.21766716090704463 1104 80-81 0.12580343246617076 1104 82-83 0.23183761104321476 1104 84-85 0.2656394386488081 1104 86-87 0.18286994900866205 1104 88-89 0.15350519475838809 1104 90-91 0.19182785939443292 1104 92-93 0.29148813518207817 1104 94-95 0.31834117589682975 1104 96-97 0.6092331031066571 1104 98-99 0.6341121919752659 1104 100-101 0.6801917549589831 1105 1 0.0721097550705494 1105 2 0.09316522031824093 1105 3 0.08577989074570525 1105 4 0.06051431006779495 1105 5 0.03618673637154757 1105 6 0.02476202038583608 1105 7 9.890959620051376E-4 1105 8 0.036094225016384485 1105 9 0.03174013220141347 1105 10-11 0.05125366951005361 1105 12-13 0.11779885027291925 1105 14-15 0.10234412901403545 1105 16-17 0.11325214274483386 1105 18-19 0.08105018877803616 1105 20-21 0.12013749170316146 1105 22-23 0.1277280106071217 1105 24-25 0.11160192265331403 1105 26-27 0.08615738558039965 1105 28-29 -0.11522150898509409 1105 30-31 0.10690382503604923 1105 32-33 0.15373347354451283 1105 34-35 0.1833426893280219 1105 36-37 0.22308557710707788 1105 38-39 0.05378132096360133 1105 40-41 0.10816824751083232 1105 42-43 0.03588234684451663 1105 44-45 0.12786572944035868 1105 46-47 0.1394175764080572 1105 48-49 0.22898009074999948 1105 50-51 0.18786655857860524 1105 52-53 0.17771744028555503 1105 54-55 0.2480006834684758 1105 56-57 0.1882474702016239 1105 58-59 0.3521747711682153 1105 60-61 0.34316736859440056 1105 62-63 0.35394793898315413 1105 64-65 0.2746200774117682 1105 66-67 0.2643046126157955 1105 68-69 0.3331504169503674 1105 70-71 0.4311828115905527 1105 72-73 0.308425083559257 1105 74-75 0.296221850783688 1105 76-77 0.2076547997314826 1105 78-79 0.233458895767928 1105 80-81 0.296792589585646 1105 82-83 0.4337016999684771 1105 84-85 0.42035438682120585 1105 86-87 0.4141550690304818 1105 88-89 0.3814610730999064 1105 90-91 0.3465520137861411 1105 92-93 0.30413168400913904 1105 94-95 0.3504575834254702 1105 96-97 0.5531590441868524 1105 98-99 0.4489873965579143 1105 100-101 0.5625805983850576 1106 1 -0.11049553669030843 1106 2 -0.015545910633861126 1106 3 -0.03486177847651817 1106 4 -0.02628188752642302 1106 5 -0.09852899392311087 1106 6 -0.04791242710025756 1106 7 0.0062506206467105585 1106 8 -0.10260138712868638 1106 9 -0.06541120961571067 1106 10-11 -0.046949646070153506 1106 12-13 -0.13694629157215132 1106 14-15 -0.29218867077613453 1106 16-17 -0.13385679225964964 1106 18-19 -0.09472298627186149 1106 20-21 -0.004246276538722782 1106 22-23 0.03197438806186881 1106 24-25 0.01173528636632426 1106 26-27 -0.06506529174830433 1106 28-29 0.07589321228905277 1106 30-31 0.0053452215034894834 1106 32-33 -0.02598952932808629 1106 34-35 0.06232464101505286 1106 36-37 0.06247884010350191 1106 38-39 0.10222576423053908 1106 40-41 0.04380692623311688 1106 42-43 -0.06426694633111651 1106 44-45 -0.0018812338609421886 1106 46-47 -0.027262309615146307 1106 48-49 -0.005622974197827091 1106 50-51 0.05779918456873645 1106 52-53 0.01786387543078405 1106 54-55 -0.4601035051522544 1106 56-57 -0.2547992365724028 1106 58-59 -0.11415784594644407 1106 60-61 -0.14327669183555258 1106 62-63 -0.10214254698799152 1106 64-65 -0.2759240081211658 1106 66-67 -0.3327746988870004 1106 68-69 -0.39553481951770664 1106 70-71 -0.37519904114172675 1106 72-73 -0.20113474664128006 1106 74-75 -0.18128655937645988 1106 76-77 -0.2158076925107686 1106 78-79 -0.2684422077696098 1106 80-81 -0.4358874315564911 1106 82-83 -0.39926256769683377 1106 84-85 -0.22469612050403143 1106 86-87 0.03317456589790879 1106 88-89 0.05932006390717959 1106 90-91 -0.24343156661393195 1106 92-93 -0.01451643449281903 1106 94-95 -0.16521381196233875 1106 96-97 0.030899938396014903 1106 98-99 0.20011330488501855 1106 100-101 0.2089815499137906 1107 1 0.09072653594449775 1107 2 0.07109753960116194 1107 3 0.0417865271408715 1107 4 0.022046841674331574 1107 5 -0.0028987270894944572 1107 6 0.0029857076752008993 1107 7 0.00912961034673998 1107 8 0.00510472131642814 1107 9 -0.020045642757352766 1107 10-11 0.07216613538088978 1107 12-13 0.06026592953463705 1107 14-15 0.18288742937694735 1107 16-17 0.04717050392961397 1107 18-19 0.10999114140076927 1107 20-21 0.137903086943318 1107 22-23 0.05877957024332403 1107 24-25 0.053384575583045546 1107 26-27 0.01910243658743127 1107 28-29 0.004334973776749962 1107 30-31 0.10176545888936772 1107 32-33 0.15937804752858398 1107 34-35 0.1274479523509484 1107 36-37 0.026879836035469395 1107 38-39 0.10861844656853492 1107 40-41 0.15361204041069243 1107 42-43 0.14281376413671154 1107 44-45 0.21640474166114387 1107 46-47 0.31363681272257793 1107 48-49 0.24842337591947228 1107 50-51 0.24081492073140964 1107 52-53 0.14331638264211932 1107 54-55 0.15238950621992586 1107 56-57 0.26208570037658063 1107 58-59 0.28166860179526054 1107 60-61 0.2940406071642272 1107 62-63 0.3159250552155868 1107 64-65 0.3930356893518905 1107 66-67 0.29261923621256614 1107 68-69 0.3078373806521313 1107 70-71 0.36275812845727273 1107 72-73 0.32356580244650957 1107 74-75 0.32047599356234 1107 76-77 0.3091118481126749 1107 78-79 0.309831771169474 1107 80-81 0.4207889886745555 1107 82-83 0.430013041908861 1107 84-85 0.4617978796985547 1107 86-87 0.4156434018092874 1107 88-89 0.44994786024996714 1107 90-91 0.27089537799999164 1107 92-93 0.4250371461495668 1107 94-95 0.46430450335472173 1107 96-97 0.7049014344815099 1107 98-99 0.8455071195114989 1107 100-101 0.9041611047435474 1108 1 -0.0012214151977048004 1108 2 0.02065140140073396 1108 3 0.07092296313810209 1108 4 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-0.49337234049052014 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 4.0 4 20.0 5 29.0 6 62.0 7 172.0 8 401.0 9 726.0 10 1372.0 11 2176.0 12 3387.0 13 5122.0 14 6888.0 15 8977.0 16 11908.0 17 17106.0 18 17232.0 19 11757.0 20 10037.0 21 10618.0 22 11004.0 23 12037.0 24 14028.0 25 16387.0 26 19574.0 27 24160.0 28 30171.0 29 36754.0 30 45247.0 31 56882.0 32 71381.0 33 93500.0 34 130886.0 35 199637.0 36 332036.0 37 578137.0 38 543243.0 39 58671.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.10198745121222 19.011573032198253 14.457725095560228 23.428714421029294 2 15.87716513477922 19.801282955736514 39.76028984923003 24.56126206025424 3 19.47464753189397 23.52950562298793 29.563904041169604 27.431942803948502 4 13.232656406398 16.504376624859766 36.07982218072136 34.18314478802088 5 14.196367954057177 36.815517039411894 33.9477433123557 15.040371694175231 6 32.96567557218576 35.424336116727915 17.034744288219052 14.575244022867276 7 29.51593454208345 32.2158888612714 19.847431126099195 18.420745470545953 8 26.556617993445116 35.292018336220586 19.153146405098134 18.998217265236168 9 26.38365693537308 16.764774542223897 20.097769186457587 36.75379933594544 10-11 25.53082950980458 26.39868096655224 26.61681943987263 21.453670083770547 12-13 25.87534596497357 23.561201371767915 27.56681658482188 22.99663607843664 14-15 22.765251009344414 25.157342686703526 25.652568034179996 26.424838269772067 16-17 22.647448491931417 28.517173484830515 26.21685798000074 22.618520043237325 18-19 22.44227339294231 26.832060876399815 28.63788047749884 22.08778525315903 20-21 23.003185900751717 26.111391484559217 28.84560641277988 22.03981620190919 22-23 22.868263759665187 25.886010419255808 28.74805184772167 22.497673973357337 24-25 22.63590674442094 26.176753454759826 28.75681971678349 22.43052008403575 26-27 22.40972932498493 26.58615029689694 28.60743062308357 22.396689755034565 28-29 22.241395851400455 26.53637975709811 28.904099035013637 22.3181253564878 30-31 22.447311717800066 26.24184211233449 28.836600407096647 22.474245762768792 32-33 22.24278150755963 26.347990746222283 28.73142631616514 22.677801430052945 34-35 22.78027819159714 26.405965717909226 28.45768386440443 22.356072226089204 36-37 22.704000120924416 26.157273960564364 28.448266813007606 22.690459105503617 38-39 22.377725159581583 26.409631187598563 28.618797546135934 22.59384610668392 40-41 22.603681541669456 26.27707662806208 28.564628754120797 22.554613076147664 42-43 22.514337373579593 26.1936194173681 28.50240054956083 22.78964265949147 44-45 22.51613836428606 26.47697340038154 28.360673048884404 22.646215186447993 46-47 22.583094572716465 26.7489705022874 27.889059445515556 22.778875479480583 48-49 22.691103661854505 26.583398551649545 27.996060869682047 22.729436916813896 50-51 22.607404490155115 26.742462666012806 27.822826466656362 22.827306377175717 52-53 22.842211421546565 26.78140471855917 27.64245443674887 22.733929423145394 54-55 22.628859356841037 26.67939963320995 27.727602126844868 22.964138883104145 56-57 22.579798668538682 27.004224635393225 27.770574839277433 22.645401856790652 58-59 22.768798829765082 26.625699487434417 27.71914193968789 22.88635974311261 60-61 22.667948360529092 26.67939963320995 27.926028154159305 22.726623852101653 62-63 22.576187869057296 26.743407351923633 27.79897839566301 22.881426383356064 64-65 22.40692114685709 26.561712761740974 27.809453912763082 23.221912178638846 66-67 22.384626559361543 26.84115085416401 27.673272190462118 23.100950396012333 68-69 22.300232498333674 26.67987131243472 27.87150137242244 23.148394816809155 70-71 22.324019709926844 26.735723906515563 27.729113624302194 23.211142759255402 72-73 22.577027589866923 26.660191019689773 27.5197082474019 23.2430731430414 74-75 22.379714192625237 26.870016256994873 27.45958423743274 23.29068531294715 76-77 22.427032460247617 26.818058531899315 27.508309037411244 23.246599970441828 78-79 22.458647948730007 26.849086215814964 27.449528580737475 23.242737254717554 80-81 22.22770373306283 27.088385653537912 27.50419440544408 23.179716207955178 82-83 22.430899884895243 27.029421933973996 27.292149637439966 23.247528543690787 84-85 22.43439329530265 26.74512270468643 27.33661855833724 23.48386544167368 86-87 22.411479878617772 26.636576525701354 27.536341717636216 23.415601878044658 88-89 22.356829581037747 26.82914438255697 27.366881047569386 23.447144988835902 90-91 22.373997475792887 26.860238195804914 27.501408635206726 23.264355693195473 92-93 22.420156536916206 26.530578398930025 27.459437717448832 23.58982734670494 94-95 22.186473441310234 26.625804452535622 27.497497631987315 23.69022447416683 96-97 22.371032474956365 26.564536979850473 27.407659012748226 23.656771532444935 98-99 22.282726439113524 26.51995764448236 27.221166506559896 23.976149409844215 100-101 22.200097911446402 26.533246226294683 27.451690861822257 23.814965000436654 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 805.0 1 537.0 2 426.5 3 626.0 4 520.0 5 453.5 6 983.5 7 2141.0 8 3437.0 9 4072.0 10 3473.5 11 2680.0 12 2569.5 13 2692.0 14 2796.5 15 2839.0 16 2923.5 17 3037.0 18 3118.0 19 3279.0 20 3550.5 21 4080.0 22 4945.0 23 5823.5 24 6961.5 25 8671.0 26 11096.5 27 14050.5 28 16449.5 29 19333.0 30 23385.5 31 26784.0 32 30765.0 33 34911.0 34 38675.0 35 42663.0 36 47019.5 37 52098.0 38 56762.5 39 59676.5 40 62649.5 41 69224.5 42 75617.5 43 81418.0 44 87387.0 45 91313.0 46 92860.5 47 94801.5 48 98366.5 49 98990.0 50 97594.0 51 94169.5 52 89361.0 53 86732.5 54 86113.0 55 80440.0 56 71242.5 57 65302.0 58 60170.0 59 52988.5 60 43791.5 61 34911.5 62 27461.5 63 21248.5 64 16861.0 65 13329.5 66 9698.5 67 7688.0 68 5640.5 69 3500.0 70 2478.0 71 1931.5 72 1646.0 73 1303.5 74 1012.0 75 789.5 76 489.0 77 245.5 78 123.5 79 73.5 80 35.0 81 17.0 82 10.0 83 5.5 84 1.5 85 1.0 86 0.5 87 0.0 88 1.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01545086289710397 3 0.007599473327108203 4 0.0 5 4.1986040481260794E-5 6 0.0 7 0.0 8 4.1986040481260795E-4 9 5.038324857751295E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.3092322264693435E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.00581506660665462 26-27 0.022273594475308852 28-29 2.0993020240630397E-5 30-31 0.0 32-33 5.878045667376511E-4 34-35 0.01979641808691446 36-37 0.003820729683794732 38-39 0.018410878751032856 40-41 0.015849730281675946 42-43 0.008649124339139723 44-45 0.0031279600158539292 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.729092631281951E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.0993020240630397E-5 84-85 0.0029600158539288854 86-87 4.8283946553449907E-4 88-89 8.397208096252159E-5 90-91 2.5191624288756475E-4 92-93 2.3092322264693435E-4 94-95 0.0 96-97 4.1986040481260794E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2381744.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.00014477458971 #Duplication Level Percentage of deduplicated Percentage of total 1 75.10707617348201 38.3047175844372 2 13.740179589400073 14.015022965765326 3 4.508071353447299 6.897368750399783 4 2.078617017343083 4.240390752616923 5 1.130646574134727 2.8831569484882467 6 0.7154047896738102 2.189144870747953 7 0.48348958135561076 1.726062705229934 8 0.3445992327710441 1.405968860842863 9 0.2513357534866875 1.1536343833366487 >10 1.4332943075228897 13.577391360073895 >50 0.13142805867304574 4.638043415490707 >100 0.07207267819555155 6.584547899436037 >500 0.00279752777133534 0.8974634443210919 >1k 7.405220571179672E-4 0.5351126311301204 >5k 2.468406857059891E-4 0.951973427683337 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9535 0.4003368959888216 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8112 0.34059076038398756 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5039 0.21156765798507313 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 1.2595812144378237E-4 0.0 0.0 0.0 0.0 8 1.6794416192504317E-4 0.0 0.0 0.0 0.0 9 1.6794416192504317E-4 0.0 0.0 0.0 0.0 10-11 1.6794416192504317E-4 0.0 0.0 0.0 0.0 12-13 1.6794416192504317E-4 0.0 0.0 0.0 0.0 14-15 1.6794416192504317E-4 0.0 0.0 0.0 0.0 16-17 1.6794416192504317E-4 0.0 0.0 0.0 0.0 18-19 2.0993020240630395E-4 0.0 0.0 0.0 0.0 20-21 2.5191624288756475E-4 0.0 0.0 4.1986040481260794E-5 0.0 22-23 2.5191624288756475E-4 0.0 0.0 2.3092322264693438E-4 0.0 24-25 3.3588832385008635E-4 0.0 0.0 6.507836274595423E-4 0.0 26-27 3.3588832385008635E-4 0.0 0.0 0.001385539335881606 0.0 28-29 3.778743643313471E-4 0.0 0.0 0.0034428553194633844 0.0 30-31 3.778743643313471E-4 0.0 0.0 0.012427867982453194 0.0 32-33 3.778743643313471E-4 0.0 0.0 0.03071278861204227 0.0 34-35 3.9886738457197747E-4 0.0 0.0 0.059872093726277886 0.0 36-37 4.618464452938687E-4 0.0 0.0 0.11170386070039433 0.0 38-39 4.618464452938687E-4 0.0 0.0 0.18555730590693206 0.0 40-41 5.038324857751295E-4 0.0 0.0 0.26467999919386803 0.0 42-43 6.087975869782814E-4 0.0 0.0 0.34197629971986915 0.0 44-45 6.297906072189118E-4 0.0 0.0 0.43579410717524636 0.0 46-47 6.92769667940803E-4 0.0 0.0 0.5515286277618417 0.0 48-49 7.557487286626942E-4 0.0 0.0 0.6669482530448276 0.0 50-51 7.557487286626942E-4 0.0 0.0 0.7889806797036121 0.0 52-53 7.557487286626942E-4 0.0 0.0 0.9031827098126415 0.0 54-55 7.557487286626942E-4 0.0 0.0 1.0304843845518243 0.0 56-57 7.557487286626942E-4 0.0 0.0 1.1785901423494716 0.0 58-59 7.557487286626942E-4 0.0 0.0 1.3393546913522192 0.0 60-61 7.97734769143955E-4 0.0 0.0 1.503121242249377 0.0 62-63 7.97734769143955E-4 0.0 0.0 1.662878126280574 0.0 64-65 7.97734769143955E-4 0.0 0.0 1.8320818694200551 0.0 66-67 8.397208096252158E-4 0.0 0.0 2.0220687025977604 0.0 68-69 8.397208096252158E-4 0.0 0.0 2.227359447530885 0.0 70-71 8.397208096252158E-4 0.0 0.0 2.445854802195366 0.0 72-73 8.817068501064766E-4 0.0 0.0 2.6628596524227626 0.0 74-75 9.236928905877374E-4 0.0 0.0 2.876400654310455 0.0 76-77 9.236928905877374E-4 0.0 0.0 3.1124671669163435 0.0 78-79 9.236928905877374E-4 0.0 0.0 3.3657899421600304 2.0993020240630397E-5 80-81 0.001007664971550259 4.1986040481260794E-5 0.0 3.638741191328707 4.1986040481260794E-5 82-83 0.001007664971550259 4.1986040481260794E-5 0.0 3.917276583881391 4.1986040481260794E-5 84-85 0.001007664971550259 4.1986040481260794E-5 0.0 4.197386452952122 4.1986040481260794E-5 86-87 0.0010286579917908895 4.1986040481260794E-5 0.0 4.489819224904104 4.1986040481260794E-5 88-89 0.0010496510120315198 4.1986040481260794E-5 0.0 4.809458951087942 4.1986040481260794E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3965 0.0 56.08026 1 GTATCAA 7180 0.0 43.939087 1 ATCAACG 9085 0.0 34.24635 3 TCAACGC 9155 0.0 33.929054 4 TATCAAC 9230 0.0 33.8198 2 CAACGCA 9330 0.0 33.292656 5 AACGCAG 9650 0.0 32.287086 6 TGGTATC 1705 0.0 27.58787 2 ACGCAGA 11310 0.0 27.464233 7 CGCAGAG 11490 0.0 26.95131 8 GTACATG 8805 0.0 26.764532 1 TACATGG 8880 0.0 25.735907 2 GTGGTAT 2130 0.0 25.429195 1 ACATGGG 9080 0.0 24.744186 3 GCAGAGT 12940 0.0 23.52752 9 GAGTACT 7525 0.0 21.933132 12-13 CATGGGG 5060 0.0 21.68272 4 CAGAGTA 12505 0.0 20.58581 10-11 GTACTTT 8220 0.0 20.049797 14-15 AGGACCG 820 0.0 19.69325 5 >>END_MODULE