FastQCFastQC Report
Thu 26 May 2016
SRR938152_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938152_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2386541
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92610.38805115855960576No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75010.3143042587577586No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47420.19869761298884037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41550.059.4709241
GTATCAA75500.042.1878621
ATCAACG97300.032.6062933
TCAACGC98100.032.340394
TATCAAC98400.032.193452
CAACGCA99250.031.8698165
AACGCAG102200.031.0371076
ACGCAGA118700.026.4356547
TGGTATC18750.026.3827762
CGCAGAG121000.025.8120278
GTGGTAT20700.024.840581
GTACATG88400.024.559581
TACATGG89100.023.488942
GCAGAGT130050.023.4660199
GTATAGG5500.023.3727281
GAGTACT74950.022.64015612-13
ACATGGG93600.021.8514883
GTGTAGC11000.021.2085861
CAGAGTA128450.020.94492710-11
AGTACTT78100.020.47938312-13
Produced by FastQC (version 0.11.5)