##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938152_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2386541 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01481265144827 33.0 31.0 34.0 30.0 34.0 2 32.1332719613868 34.0 31.0 34.0 30.0 34.0 3 32.16052060283062 34.0 31.0 34.0 30.0 34.0 4 35.6652561175358 37.0 35.0 37.0 35.0 37.0 5 35.667652891779355 37.0 35.0 37.0 35.0 37.0 6 35.69554388548112 37.0 36.0 37.0 35.0 37.0 7 35.62982408431282 37.0 35.0 37.0 33.0 37.0 8 35.6248671194 37.0 35.0 37.0 33.0 37.0 9 37.271177825983294 39.0 37.0 39.0 34.0 39.0 10-11 37.18320866894807 39.0 37.0 39.0 34.0 39.0 12-13 37.02859012269221 39.0 37.0 39.0 33.0 39.0 14-15 38.24358684807845 40.0 38.0 41.0 33.0 41.0 16-17 38.075603561807654 40.0 38.0 41.0 33.0 41.0 18-19 38.2343144324778 40.0 38.0 41.0 33.5 41.0 20-21 38.23219462812497 40.0 38.0 41.0 34.0 41.0 22-23 38.119358519296334 40.0 38.0 41.0 33.0 41.0 24-25 38.01062185816208 40.0 38.0 41.0 33.0 41.0 26-27 37.850408394408475 40.0 38.0 41.0 33.0 41.0 28-29 37.668751971996286 40.0 38.0 41.0 32.0 41.0 30-31 37.474478753979085 40.0 37.0 41.0 32.0 41.0 32-33 37.21438496133106 40.0 37.0 41.0 31.0 41.0 34-35 36.993821602059214 40.0 36.0 41.0 31.0 41.0 36-37 36.75233360750978 39.0 36.0 41.0 30.0 41.0 38-39 36.489797996347015 39.0 35.5 41.0 30.0 41.0 40-41 36.407851572631685 39.0 35.0 41.0 30.0 41.0 42-43 36.21943746200044 39.0 35.0 41.0 29.5 41.0 44-45 35.90985195728881 39.0 35.0 41.0 28.5 41.0 46-47 35.5481594491777 39.0 35.0 40.0 27.0 41.0 48-49 35.184458595096416 39.0 35.0 40.0 26.0 41.0 50-51 34.475112097382784 37.5 33.5 39.5 24.0 40.5 52-53 34.556021036303164 38.0 34.0 40.0 24.0 40.5 54-55 34.649293894385224 38.0 34.0 40.0 24.0 41.0 56-57 34.35964582213337 38.0 34.0 40.0 23.5 41.0 58-59 33.96446782183922 38.0 33.5 40.0 22.5 41.0 60-61 33.48790236580893 37.0 33.0 40.0 20.5 41.0 62-63 32.95756871555946 36.5 32.0 39.5 18.0 41.0 64-65 32.42515988621189 36.0 31.5 39.0 14.5 40.5 66-67 31.87420413058062 35.5 31.0 39.0 9.0 40.0 68-69 31.220265857573786 35.0 30.5 38.5 7.0 40.0 70-71 30.648455023399976 35.0 29.5 37.5 2.0 40.0 72-73 29.956144059540563 34.5 29.0 37.0 2.0 39.0 74-75 29.279173917397607 34.0 28.5 36.0 2.0 39.0 76-77 28.53836368199834 34.0 26.5 36.0 2.0 38.5 78-79 27.798734654045333 33.5 26.0 35.0 2.0 37.0 80-81 27.08349678467707 33.0 25.5 35.0 2.0 37.0 82-83 26.359822018561594 32.0 23.5 35.0 2.0 36.0 84-85 25.636331619695618 32.0 20.5 35.0 2.0 36.0 86-87 24.889214976822103 31.0 18.5 34.0 2.0 35.0 88-89 24.035489228971972 31.0 9.5 34.0 2.0 35.0 90-91 23.15749656092227 30.5 3.5 34.0 2.0 35.0 92-93 22.225626335353134 29.5 2.0 34.0 2.0 35.0 94-95 21.154730004638512 29.0 2.0 34.0 2.0 35.0 96-97 19.891161517862045 28.0 2.0 34.0 2.0 35.0 98-99 18.320665976406858 25.0 2.0 33.0 2.0 35.0 100-101 14.265501619289171 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2751289248429174 1101 2 0.1958368219793627 1101 3 0.16508201295398806 1101 4 -0.010946603944020694 1101 5 0.09519037101792094 1101 6 0.06468026314336583 1101 7 0.11414005663281301 1101 8 0.1731091494510224 1101 9 0.23574542165393808 1101 10-11 0.15464855284725587 1101 12-13 0.24138467048185674 1101 14-15 0.31038198940254347 1101 16-17 0.2778915744998116 1101 18-19 0.35677613250229 1101 20-21 0.2754911624198755 1101 22-23 0.36075171446648824 1101 24-25 0.3007284113498514 1101 26-27 0.24728791662127492 1101 28-29 0.2599352137808282 1101 30-31 0.2590730308011615 1101 32-33 0.28247179484332463 1101 34-35 0.2587911361669768 1101 36-37 0.23555390935315046 1101 38-39 0.17628957892274144 1101 40-41 0.15758284677833956 1101 42-43 0.19008228349884604 1101 44-45 0.28574664511430115 1101 46-47 0.37939099952304645 1101 48-49 0.3986664380893643 1101 50-51 0.39024094277751686 1101 52-53 0.3925890643399015 1101 54-55 0.2650105208892555 1101 56-57 0.28553900500694596 1101 58-59 0.37919201898211696 1101 60-61 0.3459838475373118 1101 62-63 0.13019316037415507 1101 64-65 0.17638246731128504 1101 66-67 0.13637393630388672 1101 68-69 0.2203893187263617 1101 70-71 -0.07491338039408646 1101 72-73 0.07112504901795091 1101 74-75 0.01351683906130674 1101 76-77 -0.09500314473383398 1101 78-79 -0.1801339307650487 1101 80-81 -0.2598830628716726 1101 82-83 -0.2291722298597243 1101 84-85 -0.163673422700235 1101 86-87 -0.27157503943612227 1101 88-89 -0.15217964609767165 1101 90-91 -0.22431245392985844 1101 92-93 -0.4138502259554393 1101 94-95 -0.44736010349102884 1101 96-97 -0.35505074500257905 1101 98-99 -0.5993939203140926 1101 100-101 -1.0895783392472982 1102 1 0.17611263065546012 1102 2 0.12344612217790996 1102 3 0.13979051623649497 1102 4 0.07566427269582476 1102 5 0.1246343171773745 1102 6 0.036092973543532025 1102 7 0.044810052236812226 1102 8 0.03623411020102196 1102 9 0.0880506651328048 1102 10-11 0.16716789605575144 1102 12-13 0.11512987795538265 1102 14-15 0.239552836027201 1102 16-17 0.1898461604303563 1102 18-19 0.193074619344074 1102 20-21 0.16546695221052943 1102 22-23 0.18774654071174268 1102 24-25 -0.003935375104347827 1102 26-27 0.09336126542380896 1102 28-29 0.015787691724334252 1102 30-31 0.070156105554112 1102 32-33 -0.007852655777348616 1102 34-35 -0.01423050246243207 1102 36-37 0.018576852290486556 1102 38-39 -0.19112007359996142 1102 40-41 0.00838209768691911 1102 42-43 0.1889512794554875 1102 44-45 0.05516211388417247 1102 46-47 0.17864049913577418 1102 48-49 0.16853237616854955 1102 50-51 -0.018000592505622137 1102 52-53 -0.01020597896796005 1102 54-55 0.03583657452045941 1102 56-57 0.13544577114453915 1102 58-59 0.23993782547112374 1102 60-61 0.22359709286475038 1102 62-63 0.017053707313948507 1102 64-65 -0.09217835771983118 1102 66-67 -0.3472406954545626 1102 68-69 -0.30628075900072815 1102 70-71 -0.49159447227694386 1102 72-73 -0.5428921952209542 1102 74-75 -0.6702752429844914 1102 76-77 -0.9134052387116327 1102 78-79 -0.6459375132641334 1102 80-81 -0.5923286080160253 1102 82-83 -0.8112679467379529 1102 84-85 -0.9449353486638508 1102 86-87 -0.7919061404169305 1102 88-89 -0.6671228281903296 1102 90-91 -0.5618077640572459 1102 92-93 -1.0526966219594236 1102 94-95 -1.1679018663034384 1102 96-97 -1.264854229276004 1102 98-99 -1.2797273722696687 1102 100-101 -1.4699026529961525 1103 1 0.24583892769815918 1103 2 0.14268812745824988 1103 3 0.3038498185439096 1103 4 0.22504289512009734 1103 5 0.154077303539097 1103 6 0.1618670592475553 1103 7 0.28237234621753515 1103 8 0.19773955457776538 1103 9 0.1737082083675574 1103 10-11 0.21747175008083985 1103 12-13 0.25097391257443036 1103 14-15 0.3313624468753318 1103 16-17 0.3877599955524431 1103 18-19 0.30333437938531915 1103 20-21 0.2927153938846132 1103 22-23 0.22297134582336753 1103 24-25 0.23864519698482667 1103 26-27 0.24314821610416004 1103 28-29 0.2850621133081219 1103 30-31 0.22261915877933802 1103 32-33 0.24118344739242303 1103 34-35 0.16387306567428794 1103 36-37 0.099079812719161 1103 38-39 0.13400705298765558 1103 40-41 0.26867505514736223 1103 42-43 0.26504698956781425 1103 44-45 0.2371564195503879 1103 46-47 0.07510536454953609 1103 48-49 -0.026667862852860935 1103 50-51 0.08308261975477649 1103 52-53 0.006468303577236156 1103 54-55 -0.10689709625486898 1103 56-57 -0.1656534539886465 1103 58-59 -0.05919826051408705 1103 60-61 -0.1368061971947725 1103 62-63 -0.061571060304117964 1103 64-65 -0.2538259651622994 1103 66-67 -0.29011594345881164 1103 68-69 -0.06855892212894332 1103 70-71 -0.37742718071768167 1103 72-73 -0.4701755918246633 1103 74-75 -0.3512173857412044 1103 76-77 -0.40038130532518323 1103 78-79 -0.4895153748816732 1103 80-81 -0.5786749000595748 1103 82-83 -0.688941510656651 1103 84-85 -0.7184666557829402 1103 86-87 -0.7706221032983116 1103 88-89 -0.7204361259047829 1103 90-91 -0.49340409125101203 1103 92-93 -0.6869633886089055 1103 94-95 -0.8829625589968835 1103 96-97 -0.9986197148956677 1103 98-99 -0.9573140370616571 1103 100-101 -1.074848873177059 1104 1 0.2367904054917389 1104 2 0.17543214967595588 1104 3 0.2202873086656112 1104 4 0.1805369229825473 1104 5 0.2090723361657041 1104 6 0.12485843408103392 1104 7 0.13902072391854148 1104 8 0.2683581054594768 1104 9 0.3678653354194239 1104 10-11 0.29725454038995025 1104 12-13 0.28986647054593817 1104 14-15 0.3202327081140197 1104 16-17 0.35784652945476836 1104 18-19 0.4029547704530785 1104 20-21 0.25172440289362186 1104 22-23 0.3772709461755994 1104 24-25 0.39265804441873087 1104 26-27 0.3286832812444871 1104 28-29 0.31710223613772115 1104 30-31 0.29432573377538773 1104 32-33 0.3963754815318268 1104 34-35 0.47115788809069414 1104 36-37 0.32664929949917365 1104 38-39 0.3741843543491683 1104 40-41 0.4654476736305071 1104 42-43 0.4701591506010274 1104 44-45 0.34317907754672916 1104 46-47 0.1489750048665428 1104 48-49 0.19506975822686456 1104 50-51 0.18509863953395467 1104 52-53 0.2610481653939445 1104 54-55 0.18981258334954276 1104 56-57 0.09993930041989785 1104 58-59 0.10745147192510984 1104 60-61 0.19107939516450045 1104 62-63 0.06942986543365492 1104 64-65 -0.14436255438310042 1104 66-67 -0.04399698615564418 1104 68-69 0.0543003092829224 1104 70-71 -0.08221928485188812 1104 72-73 -0.05298029502410273 1104 74-75 -0.11015950455174917 1104 76-77 -0.3896015405980542 1104 78-79 -0.32596488830487047 1104 80-81 -0.37511447873599835 1104 82-83 -0.6065229078433099 1104 84-85 -0.48317149219830213 1104 86-87 -0.37285036539499217 1104 88-89 -0.40409657206523164 1104 90-91 -0.5701085212449151 1104 92-93 -0.8076604014111801 1104 94-95 -0.8208816811265329 1104 96-97 -0.8028050092125412 1104 98-99 -1.1911362050943524 1104 100-101 -1.4280508533527243 1105 1 0.21670005164981632 1105 2 0.15651629889059393 1105 3 0.2315193688311723 1105 4 0.07340792626759907 1105 5 0.19339525022513016 1105 6 0.17134173401477426 1105 7 0.1369828373135249 1105 8 0.149953448786313 1105 9 0.1444433114955217 1105 10-11 0.2169434531779828 1105 12-13 0.2217802807533431 1105 14-15 0.26433642825286796 1105 16-17 0.2529543097570226 1105 18-19 0.25102940899195403 1105 20-21 0.16115955950569827 1105 22-23 0.3002188088976112 1105 24-25 0.26801135076325266 1105 26-27 0.3300093070372583 1105 28-29 0.19266573654437735 1105 30-31 0.25703687653671636 1105 32-33 0.33308069364481696 1105 34-35 0.20270481411692032 1105 36-37 0.26368447053685884 1105 38-39 0.06372072707248577 1105 40-41 0.11477804002485925 1105 42-43 0.2629880074198425 1105 44-45 0.306217757022047 1105 46-47 0.2467548065359253 1105 48-49 0.32508514733886074 1105 50-51 0.23330466954676155 1105 52-53 0.2925595118362736 1105 54-55 0.18129932013113148 1105 56-57 0.21870648129497994 1105 58-59 0.17369812725583245 1105 60-61 0.22310102066166593 1105 62-63 0.27834177070690913 1105 64-65 0.08465881328318403 1105 66-67 0.2263187678489409 1105 68-69 0.1445992477625353 1105 70-71 0.09871608679866384 1105 72-73 0.012168560588989408 1105 74-75 0.007407336352610372 1105 76-77 -0.01849033259851396 1105 78-79 -0.1322383601994197 1105 80-81 -0.15337965913417761 1105 82-83 -0.1609970808871175 1105 84-85 -0.26003179038858804 1105 86-87 -0.2345402502969236 1105 88-89 -0.25279183481319123 1105 90-91 -0.33298701185238855 1105 92-93 -0.4732023549079649 1105 94-95 -0.5468666731090153 1105 96-97 -0.7356007732504608 1105 98-99 -1.091628021520922 1105 100-101 -1.013912301823959 1106 1 0.24282410909825813 1106 2 0.20836688746506127 1106 3 0.26594315869039065 1106 4 0.22859850745189902 1106 5 0.18094240318140464 1106 6 0.13854479728195912 1106 7 0.3058691951359407 1106 8 0.20300697069086482 1106 9 0.4237209830226476 1106 10-11 0.3525211454138386 1106 12-13 0.40850882996499394 1106 14-15 0.3989957291100481 1106 16-17 -0.0466229886069911 1106 18-19 0.33101066962548487 1106 20-21 0.46043384048655867 1106 22-23 0.43894392289712414 1106 24-25 0.4748400530570436 1106 26-27 0.4506192602192982 1106 28-29 0.41850190210049476 1106 30-31 0.3648372647238034 1106 32-33 0.2630749479885708 1106 34-35 0.17257473489588904 1106 36-37 0.3221934219850482 1106 38-39 0.4822249886033916 1106 40-41 0.46189384240676645 1106 42-43 0.21301369030022244 1106 44-45 0.5142833996219309 1106 46-47 0.54274929233015 1106 48-49 0.5272347942753939 1106 50-51 0.5046526248646401 1106 52-53 0.5332801754900274 1106 54-55 0.6237791088209264 1106 56-57 0.2894457624991773 1106 58-59 0.39317356711323015 1106 60-61 0.6346168993816192 1106 62-63 0.363744047854631 1106 64-65 0.1638541574743826 1106 66-67 0.21505992095951498 1106 68-69 0.10562562905484185 1106 70-71 0.11430123011522753 1106 72-73 0.031227491125427775 1106 74-75 0.16436130613411137 1106 76-77 -0.2120658565089677 1106 78-79 -0.30685271094026234 1106 80-81 -0.16382010530570312 1106 82-83 -0.110328412376969 1106 84-85 -0.14768867928830787 1106 86-87 -0.1562570036560018 1106 88-89 -0.5204765789792205 1106 90-91 -0.5655574722763923 1106 92-93 -0.4466593518178996 1106 94-95 -0.711820504832346 1106 96-97 -0.39704630321111267 1106 98-99 -0.5600392968129739 1106 100-101 -0.8767331348019951 1107 1 0.037053566178933295 1107 2 0.06816081978978872 1107 3 0.15161085836371768 1107 4 0.1255217866953231 1107 5 0.05401066166940183 1107 6 0.10739068869809643 1107 7 0.18113249747907645 1107 8 0.21072955313127295 1107 9 0.09376741114482456 1107 10-11 0.04213647431782164 1107 12-13 0.12525764162615616 1107 14-15 0.0414604982464013 1107 16-17 0.08094418541461579 1107 18-19 0.16196036868129227 1107 20-21 0.16909937503891825 1107 22-23 0.24286420222821903 1107 24-25 0.25139032541249406 1107 26-27 0.1687358527458258 1107 28-29 0.2138829138371321 1107 30-31 0.1288103951151669 1107 32-33 0.21490072055176057 1107 34-35 0.18570964327849282 1107 36-37 0.1583037707296242 1107 38-39 0.03902663226565295 1107 40-41 0.10591726993048667 1107 42-43 0.09852729663701609 1107 44-45 0.15274002668365227 1107 46-47 0.09826806370023888 1107 48-49 0.24839542109315715 1107 50-51 0.0378423474013303 1107 52-53 0.030831072986032382 1107 54-55 -0.059099377576558254 1107 56-57 -0.007206209552471421 1107 58-59 -0.10596638813585457 1107 60-61 -0.06407202076429286 1107 62-63 0.0324570016056569 1107 64-65 -0.009370672041399075 1107 66-67 0.052261311516080156 1107 68-69 -0.11006673317599436 1107 70-71 -0.2606569350209895 1107 72-73 -0.26143130029489825 1107 74-75 -0.3436623157930754 1107 76-77 -0.5568662308309378 1107 78-79 -0.5616066380808924 1107 80-81 -0.49870904756024714 1107 82-83 -0.5958797558574034 1107 84-85 -0.7451277947906476 1107 86-87 -0.639745478579858 1107 88-89 -0.709997374432465 1107 90-91 -0.5939612482778536 1107 92-93 -0.6963211469623865 1107 94-95 -0.9156945656920641 1107 96-97 -1.0813809096965699 1107 98-99 -1.253395081840857 1107 100-101 -1.2128411964469539 1108 1 -0.03472068893601943 1108 2 -0.033920059676248115 1108 3 -0.06251632830468878 1108 4 -0.019880756601800442 1108 5 -0.058792254615546824 1108 6 -0.07069873022613393 1108 7 0.012131930363082688 1108 8 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sequence quality scores pass #Quality Count 2 7476.0 3 2152.0 4 2064.0 5 2231.0 6 2748.0 7 3359.0 8 4132.0 9 5179.0 10 6592.0 11 8225.0 12 10113.0 13 12648.0 14 14713.0 15 17671.0 16 20818.0 17 26546.0 18 25962.0 19 22122.0 20 23206.0 21 25386.0 22 27919.0 23 30928.0 24 35015.0 25 39807.0 26 45006.0 27 51400.0 28 59109.0 29 68561.0 30 80831.0 31 96866.0 32 117712.0 33 147179.0 34 189255.0 35 251533.0 36 327426.0 37 367211.0 38 196086.0 39 11353.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.819128499384405 18.541313654449613 14.131902569047721 23.50765527711826 2 15.602499398376835 19.51770365258631 40.31973651001545 24.560060439021406 3 19.433934945326122 23.529890694214494 29.29785354409688 27.738320816362506 4 12.949974133449583 16.179873432220617 36.40323623482045 34.466916199509356 5 14.272902658322714 36.78914586451644 33.77113191267821 15.166819564482642 6 32.94548028671427 35.2280933635185 17.144831686360916 14.681594663406312 7 29.47888444208463 32.170569804217024 19.953451962971435 18.39709379072691 8 26.595156298418043 35.082473418429586 19.256139538725837 19.06623074442654 9 26.592943632462745 16.777646976891315 20.08247168365913 36.54693770698681 10-11 25.57766026567318 26.343015288644523 26.61467406190372 21.464650383778576 12-13 25.97128912713079 23.603260079263045 27.496306380807155 22.92914441279901 14-15 22.897330770389523 25.023424416865243 25.722884008329988 26.356360804415246 16-17 22.657632850697624 28.471206022823708 26.201066393092674 22.670094733385994 18-19 22.49151128943435 26.752744342170825 28.65503369784701 22.10071067054782 20-21 23.11778147576573 26.05594059946007 28.795172027770157 22.031105897004043 22-23 22.930599667759786 25.851189722680402 28.709135724094093 22.50907488546572 24-25 22.6329287323267 26.117510108026114 28.71080462743504 22.538756532212144 26-27 22.45779604456993 26.481775545625762 28.603912290302112 22.456516119502197 28-29 22.30010906488868 26.47933546568232 28.855603247989727 22.364952221439275 30-31 22.4769247288556 26.173707738677955 28.79652488891238 22.552842643554065 32-33 22.295981106910503 26.337487838349798 28.66295898036389 22.703572074375806 34-35 22.839289990860863 26.368427652961035 28.384784152339666 22.40749820383844 36-37 22.747389946628843 26.070610467725253 28.38732378417687 22.794675801469026 38-39 22.41666161836472 26.390957411686333 28.52034165230608 22.672039317642863 40-41 22.65015702620192 26.2588981689116 28.538141717902914 22.552803086983563 42-43 22.58541955227939 26.149725591201644 28.386399956562506 22.87845489995646 44-45 22.572312155023262 26.49142608026778 28.24081380713894 22.69544795757002 46-47 22.605511279550914 26.704530685996254 27.787753271090725 22.902204763362107 48-49 22.764305177111716 26.514399496960806 27.891699853280233 22.82959547264724 50-51 22.65149534859395 26.773207716686727 27.69193875769234 22.883358177026984 52-53 22.828786043171228 26.81866982358438 27.55911204773602 22.79343208550838 54-55 22.68104575684013 26.664522994966493 27.690634635124788 22.963796613068585 56-57 22.632526184182236 26.991219617693545 27.702171142220898 22.674083055903317 58-59 22.81866040893927 26.61563921783997 27.665562341281614 22.900138031939143 60-61 22.782336449009474 26.592387024864976 27.89053767682215 22.7347388493034 62-63 22.644017332031748 26.6310721799761 27.78788950379083 22.93702098420132 64-65 22.531846566389124 26.50580844314944 27.784931876077046 23.17741311438439 66-67 22.484107310940875 26.67488119912333 27.683700957763623 23.157310532172175 68-69 22.45970369289522 26.56443006543511 27.797198105794735 23.17866813587493 70-71 22.441979949296783 26.59686414816374 27.78017677789316 23.180979124646317 72-73 22.754622312367783 26.52889935680453 27.510294397550304 23.20618393327738 74-75 22.572450409802823 26.760334884875203 27.493416933681804 23.173797771640174 76-77 22.699562889235306 26.655290624488554 27.425321410212916 23.219825076063223 78-79 22.768170730685085 26.693098852463137 27.36500305953948 23.1737273573123 80-81 22.494737316382373 26.946197508070846 27.447496282853134 23.111568892693647 82-83 22.77307872606115 26.889924419578108 27.207966969006513 23.12902988535422 84-85 22.831287241165064 26.54166243198756 27.268218113390212 23.358832213457166 86-87 22.780548628428928 26.448584421299692 27.468136381734737 23.302730568536642 88-89 22.82153352936663 26.53256708997056 27.27502957061496 23.370869810047846 90-91 22.85088358640333 26.618587037796377 27.316835805305818 23.213693570494478 92-93 22.973178685194473 26.19772909121713 27.32764795378337 23.501444269805035 94-95 22.82711867107617 26.282002762196367 27.323602814865538 23.567275751861924 96-97 23.082664363271185 26.204219490667263 27.12600397922751 23.58711216683404 98-99 23.018930101194066 26.137932754883874 26.896432308060287 23.94670483586177 100-101 23.78771753489716 25.245680980495944 27.00440078554137 23.962200699065527 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 799.0 1 550.5 2 453.0 3 612.0 4 510.0 5 483.0 6 924.5 7 1973.5 8 3189.0 9 3674.0 10 3200.5 11 2584.5 12 2530.5 13 2681.5 14 2797.5 15 2938.0 16 3008.5 17 3082.0 18 3159.5 19 3414.0 20 3776.5 21 4292.5 22 5046.5 23 6143.0 24 7512.0 25 9144.5 26 11629.0 27 14498.0 28 16822.5 29 19676.0 30 23468.5 31 26960.5 32 30751.5 33 34926.0 34 38987.5 35 42483.5 36 46716.0 37 51758.0 38 56083.0 39 59213.5 40 62338.0 41 68437.0 42 74688.0 43 80126.0 44 85903.5 45 90122.5 46 91472.0 47 93196.0 48 96991.5 49 97690.5 50 95582.0 51 92537.0 52 89074.0 53 86803.0 54 85100.5 55 79884.5 56 71916.0 57 65969.0 58 60537.0 59 53349.5 60 45096.0 61 37019.5 62 29635.0 63 23233.5 64 18505.0 65 14844.5 66 11222.5 67 8730.0 68 6603.0 69 4439.0 70 3307.0 71 2583.5 72 2026.0 73 1594.5 74 1228.5 75 970.0 76 692.0 77 400.5 78 235.0 79 157.0 80 88.0 81 45.5 82 26.0 83 17.5 84 12.5 85 10.0 86 8.0 87 6.0 88 4.5 89 5.0 90 5.5 91 4.0 92 1.5 93 1.5 94 2.5 95 3.0 96 2.5 97 1.5 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1457758320514921 2 0.055603486384688136 3 0.15822062139305382 4 0.1321577965767192 5 0.20012226900773966 6 0.20100220360764806 7 0.16995308272516582 8 0.0717356207163422 9 0.122981335749103 10-11 0.10408369267487967 12-13 0.1556855717123653 14-15 0.14011910962350949 16-17 0.13716504346667416 18-19 0.1352794693240133 20-21 0.13672507616671994 22-23 0.20240590880274004 24-25 0.22061217469132102 26-27 0.15038501328910756 28-29 0.12979035348648943 30-31 0.10001923285625514 32-33 0.12671058238681004 34-35 0.09595477303763061 36-37 0.0882867715241431 38-39 0.052398010342164666 40-41 0.05989840526519344 42-43 0.06304102883629488 44-45 0.040372237476749825 46-47 0.05241896116597201 48-49 0.043619615166887976 50-51 0.05424168283721084 52-53 0.028053153078032182 54-55 0.051350469151797515 56-57 0.027403677540004553 58-59 0.051266665856568146 60-61 0.03840286003885959 62-63 0.05223040375170593 64-65 0.0403093850053278 66-67 0.03903138475307988 68-69 0.06633030817404771 70-71 0.05396932212771539 72-73 0.05292178093734824 74-75 0.0446043038858331 76-77 0.02231262735482022 78-79 0.023506824311838766 80-81 0.026754202001976923 82-83 0.02260593888812302 84-85 0.02046895485977404 86-87 0.02476387374027934 88-89 0.06586939005028616 90-91 0.11097651370749549 92-93 0.081498704610564 94-95 0.07728758902528807 96-97 0.06938912844991978 98-99 0.05746810970354165 100-101 0.13477664955263707 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2386541.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.20959368573151 #Duplication Level Percentage of deduplicated Percentage of total 1 76.95248853329927 40.94610647962767 2 12.804292750448628 13.626224293690584 3 4.130581313246692 6.593596600911952 4 1.9307196610224546 4.109312347362323 5 1.0567454936786818 2.811449917393521 6 0.6460021586388859 2.062410742876835 7 0.42104005450742654 1.5682359158030894 8 0.30771412170953 1.309867471002066 9 0.23714716515333933 1.1356653871379028 >10 1.3264819109300348 13.078869971297822 >50 0.11851047984299562 4.399325857316668 >100 0.06504001298446033 6.197011543199875 >500 0.0023680569787093574 0.7978112089418097 >1k 7.104170936124965E-4 0.6600895477612535 >5k 1.5787046524722145E-4 0.7040227156766438 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9261 0.38805115855960576 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7501 0.3143042587577586 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4742 0.19869761298884037 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.1901647614685854E-5 0.0 10-11 0.0 0.0 0.0 4.1901647614685854E-5 0.0 12-13 0.0 0.0 0.0 4.1901647614685854E-5 0.0 14-15 4.1901647614685854E-5 0.0 0.0 4.1901647614685854E-5 0.0 16-17 4.1901647614685854E-5 0.0 0.0 8.380329522937171E-5 0.0 18-19 4.1901647614685854E-5 0.0 0.0 1.2570494284405758E-4 0.0 20-21 4.1901647614685854E-5 0.0 0.0 1.6760659045874342E-4 0.0 22-23 4.1901647614685854E-5 0.0 0.0 2.5140988568811515E-4 0.0 24-25 4.1901647614685854E-5 0.0 0.0 5.028197713762303E-4 0.0 26-27 4.1901647614685854E-5 0.0 0.0 0.0012780002522479188 0.0 28-29 1.2570494284405758E-4 0.0 0.0 0.004420623823349358 0.0 30-31 1.2570494284405758E-4 0.0 0.0 0.013659937122387588 0.0 32-33 1.2570494284405758E-4 0.0 0.0 0.03257853102041825 0.0 34-35 1.2570494284405758E-4 0.0 0.0 0.06151161869835883 0.0 36-37 1.2570494284405758E-4 0.0 0.0 0.11334395679772524 0.0 38-39 1.2570494284405758E-4 0.0 0.0 0.19054774252778395 0.0 40-41 1.2570494284405758E-4 0.0 0.0 0.2683172005006409 0.0 42-43 1.2570494284405758E-4 0.0 0.0 0.347930330968544 0.0 44-45 1.6760659045874342E-4 0.0 0.0 0.44313087434911025 0.0 46-47 2.93311533302801E-4 0.0 0.0 0.554735912770826 0.0 48-49 2.93311533302801E-4 0.0 0.0 0.6714529522015336 0.0 50-51 2.93311533302801E-4 0.0 0.0 0.7906421888415074 0.0 52-53 2.93311533302801E-4 0.0 0.0 0.9107532617290044 0.0 54-55 2.93311533302801E-4 0.0 0.0 1.0392865657870534 0.0 56-57 3.7711482853217273E-4 0.0 0.0 1.1868222670383624 0.0 58-59 3.7711482853217273E-4 0.0 0.0 1.3464884952741227 2.0950823807342927E-5 60-61 3.7711482853217273E-4 0.0 0.0 1.5092763962571771 4.1901647614685854E-5 62-63 3.7711482853217273E-4 0.0 0.0 1.6745364944494983 4.1901647614685854E-5 64-65 3.7711482853217273E-4 0.0 0.0 1.8456837741316825 8.380329522937171E-5 66-67 4.609181237615444E-4 0.0 0.0 2.035225877116714 8.380329522937171E-5 68-69 4.609181237615444E-4 0.0 0.0 2.2415495899714273 8.380329522937171E-5 70-71 4.609181237615444E-4 0.0 0.0 2.4601714364010503 8.380329522937171E-5 72-73 4.609181237615444E-4 0.0 0.0 2.6768029545689767 8.380329522937171E-5 74-75 4.609181237615444E-4 0.0 0.0 2.891276537884746 8.380329522937171E-5 76-77 4.609181237615444E-4 0.0 0.0 3.1294874045742356 8.380329522937171E-5 78-79 4.609181237615444E-4 0.0 0.0 3.389445226375746 8.380329522937171E-5 80-81 6.494755380276307E-4 0.0 0.0 3.6643200347280853 8.380329522937171E-5 82-83 6.704263618349737E-4 0.0 0.0 3.938377760951939 8.380329522937171E-5 84-85 6.913771856423166E-4 0.0 0.0 4.216730406056296 8.380329522937171E-5 86-87 7.123280094496596E-4 0.0 0.0 4.500949281826711 1.0475411903671464E-4 88-89 7.542296570643455E-4 0.0 0.0 4.808758785204193 1.2570494284405758E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4155 0.0 59.470924 1 GTATCAA 7550 0.0 42.187862 1 ATCAACG 9730 0.0 32.606293 3 TCAACGC 9810 0.0 32.34039 4 TATCAAC 9840 0.0 32.19345 2 CAACGCA 9925 0.0 31.869816 5 AACGCAG 10220 0.0 31.037107 6 ACGCAGA 11870 0.0 26.435654 7 TGGTATC 1875 0.0 26.382776 2 CGCAGAG 12100 0.0 25.812027 8 GTGGTAT 2070 0.0 24.84058 1 GTACATG 8840 0.0 24.55958 1 TACATGG 8910 0.0 23.48894 2 GCAGAGT 13005 0.0 23.466019 9 GTATAGG 550 0.0 23.372728 1 GAGTACT 7495 0.0 22.640156 12-13 ACATGGG 9360 0.0 21.851488 3 GTGTAGC 1100 0.0 21.208586 1 CAGAGTA 12845 0.0 20.944927 10-11 AGTACTT 7810 0.0 20.479383 12-13 >>END_MODULE