FastQCFastQC Report
Thu 26 May 2016
SRR938152_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938152_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2386541
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91570.38369338720767837No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78130.32737757281354063No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49090.20569518814049287No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42000.060.6222881
GTATCAA72700.042.863361
ATCAACG92900.033.2287753
TCAACGC94350.032.665034
TATCAAC95800.032.4266242
CAACGCA96400.032.0196535
AACGCAG100100.030.8835496
ACGCAGA116150.026.4933017
CGCAGAG116550.026.2393998
GTGGTAT20250.023.6926231
GCAGAGT129800.023.231619
GAGTACT76650.023.07876812-13
TGGTATC18600.022.9836522
GTACATG84550.022.8102071
TACATGG83700.022.5864052
CAGAGTA126300.021.56373810-11
GTACTTT81050.021.5622114-15
ACATGGG86450.020.9852923
AGTACTT79250.019.56511312-13
AGAGTAC127100.019.16751510-11
Produced by FastQC (version 0.11.5)