##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938152_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2386541 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.54353644039637 34.0 31.0 34.0 31.0 34.0 2 32.756843900859025 34.0 31.0 34.0 31.0 34.0 3 32.81460532209587 34.0 31.0 34.0 31.0 34.0 4 36.258615712028416 37.0 35.0 37.0 35.0 37.0 5 36.23091746590568 37.0 37.0 37.0 35.0 37.0 6 36.261358174864796 37.0 37.0 37.0 35.0 37.0 7 36.21990445586311 37.0 37.0 37.0 35.0 37.0 8 36.210352556272866 37.0 37.0 37.0 35.0 37.0 9 37.903309434030255 39.0 38.0 39.0 35.0 39.0 10-11 37.88292637754809 39.0 38.0 39.0 35.0 39.0 12-13 37.800112380218906 39.0 38.0 39.0 35.0 39.0 14-15 39.10446918783293 40.0 38.5 41.0 36.0 41.0 16-17 38.99341222296202 40.0 38.0 41.0 36.0 41.0 18-19 39.228145462407724 40.5 39.0 41.0 36.0 41.0 20-21 39.209277150486834 41.0 39.0 41.0 36.0 41.0 22-23 39.11545349524689 40.0 39.0 41.0 35.5 41.0 24-25 39.02264344086274 40.0 38.5 41.0 35.0 41.0 26-27 38.86548293115433 40.0 38.0 41.0 35.0 41.0 28-29 38.74917170918077 40.0 38.0 41.0 35.0 41.0 30-31 38.5414780219573 40.0 38.0 41.0 34.0 41.0 32-33 38.35781367259142 40.0 38.0 41.0 34.0 41.0 34-35 38.21724663435491 40.0 38.0 41.0 34.0 41.0 36-37 38.237582551483506 40.0 38.0 41.0 34.0 41.0 38-39 38.11863403980908 40.0 38.0 41.0 34.0 41.0 40-41 37.96384327778153 40.0 38.0 41.0 33.0 41.0 42-43 37.750683101610235 40.0 37.0 41.0 33.0 41.0 44-45 37.49122390941534 40.0 37.0 41.0 33.0 41.0 46-47 37.124663058376115 40.0 37.0 41.0 31.5 41.0 48-49 36.76630696895633 39.5 36.0 41.0 31.0 41.0 50-51 36.41211988396596 39.0 35.5 41.0 30.0 41.0 52-53 35.920084968161035 39.0 35.0 40.0 29.0 41.0 54-55 35.61458173146826 38.5 35.0 40.0 28.0 41.0 56-57 35.28907485771248 38.0 34.5 40.0 28.0 41.0 58-59 34.86793564409746 38.0 34.0 40.0 26.5 41.0 60-61 34.4086638360707 37.5 34.0 40.0 26.0 41.0 62-63 33.946138365106655 37.0 33.0 39.5 25.5 41.0 64-65 33.46246261849262 36.0 32.5 39.0 23.5 40.5 66-67 32.90613989032663 36.0 32.0 39.0 22.0 40.0 68-69 32.33574952200695 35.0 31.0 38.0 20.5 40.0 70-71 31.735735736364887 35.0 31.0 37.5 18.5 40.0 72-73 31.140417658862766 34.5 30.0 37.0 16.5 39.0 74-75 30.525878457566833 34.0 29.5 36.0 12.0 39.0 76-77 29.154782172189794 32.5 28.0 35.0 9.0 37.0 78-79 29.852617030254244 34.0 29.0 35.0 7.5 37.0 80-81 29.51781720909048 34.0 29.0 35.0 4.5 37.0 82-83 28.954614858910865 34.0 29.0 35.0 2.0 36.5 84-85 28.362645980102585 33.5 27.5 35.0 2.0 36.0 86-87 27.70655480044131 33.0 26.5 35.0 2.0 35.5 88-89 26.98389007354158 32.5 25.5 35.0 2.0 35.0 90-91 26.44858772591797 32.0 25.0 34.5 2.0 35.0 92-93 25.618830139519915 32.0 21.5 34.0 2.0 35.0 94-95 24.78727727702981 31.0 19.0 34.0 2.0 35.0 96-97 23.722537974415694 31.0 4.5 34.0 2.0 35.0 98-99 22.256569026050673 30.5 2.0 34.0 2.0 35.0 100-101 17.804949087403067 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.167277254915966 1101 2 0.15833330456045758 1101 3 0.14976260505058292 1101 4 0.07789052292645238 1101 5 0.07112226607451788 1101 6 0.034879903740467455 1101 7 0.04458198088029519 1101 8 0.11708197074722904 1101 9 0.04091435400676602 1101 10-11 0.14502906164840823 1101 12-13 0.26474370692550764 1101 14-15 0.24729895605619134 1101 16-17 0.26071114208317425 1101 18-19 0.27653851475590585 1101 20-21 0.27362120801740986 1101 22-23 0.25442341680558656 1101 24-25 0.31112273694513704 1101 26-27 0.27353582478105665 1101 28-29 0.33579990147209315 1101 30-31 0.22870995578131925 1101 32-33 0.3096282387140974 1101 34-35 0.3653375658012905 1101 36-37 0.43655539912452923 1101 38-39 0.2855986706227043 1101 40-41 0.2623023389511445 1101 42-43 0.39184554838253405 1101 44-45 0.41282951757035846 1101 46-47 0.43233176933934203 1101 48-49 0.6110332282811797 1101 50-51 0.48198467241552834 1101 52-53 0.2937379219341665 1101 54-55 0.276899973005051 1101 56-57 0.3893028141528063 1101 58-59 0.40652415448106893 1101 60-61 0.4935782431236504 1101 62-63 0.5466068038452292 1101 64-65 0.43099546311207604 1101 66-67 0.21492538704118402 1101 68-69 0.34107622066903076 1101 70-71 0.2951741266958585 1101 72-73 0.3152956113799661 1101 74-75 0.10015332470130645 1101 76-77 -0.03527963113417698 1101 78-79 -0.0980254882076288 1101 80-81 -0.010805225829869869 1101 82-83 0.17729179013877072 1101 84-85 -0.028590792084067118 1101 86-87 -0.23213307181002563 1101 88-89 -0.1628421105369533 1101 90-91 -0.1744728395291979 1101 92-93 -0.32455337737725287 1101 94-95 -0.46390657855145534 1101 96-97 -0.44049821564549774 1101 98-99 -0.24391734136168353 1101 100-101 -0.6494817170052407 1102 1 0.01922904705114803 1102 2 0.07751854687993642 1102 3 0.03400769255998881 1102 4 0.004602507583932436 1102 5 0.017465292616378747 1102 6 -0.030230020432547633 1102 7 -0.0490166046702214 1102 8 0.014963114480345041 1102 9 -0.04525651999225744 1102 10-11 0.06452407709881669 1102 12-13 0.11020676844213995 1102 14-15 0.10417430930625216 1102 16-17 0.09784414445508816 1102 18-19 0.10711616213867359 1102 20-21 0.039389445382134625 1102 22-23 -0.014510987777846651 1102 24-25 -0.04376656595864148 1102 26-27 -0.04165139171312404 1102 28-29 -0.03487474777373478 1102 30-31 -0.007901564961194651 1102 32-33 -0.030053397435523266 1102 34-35 -0.09556922610040886 1102 36-37 -0.004585089330745973 1102 38-39 0.09995479338223134 1102 40-41 -0.011668545997629565 1102 42-43 -0.07379358665198765 1102 44-45 -0.13227719414055628 1102 46-47 0.007026343901479493 1102 48-49 0.09794868497412779 1102 50-51 0.09762485411689426 1102 52-53 0.06985588289832378 1102 54-55 0.11470948446927309 1102 56-57 0.039028104361321425 1102 58-59 0.1494251365766317 1102 60-61 0.1700996136453199 1102 62-63 0.1730779746145572 1102 64-65 0.18221362229882487 1102 66-67 -0.10554306883536668 1102 68-69 0.017772505323769394 1102 70-71 -0.056972707781028475 1102 72-73 -0.11176499385111072 1102 74-75 -0.14228953936565247 1102 76-77 -0.20502564534630707 1102 78-79 -0.3290067464300037 1102 80-81 -0.483008572265323 1102 82-83 -0.5432894239625696 1102 84-85 -0.5434258856963439 1102 86-87 -0.7884346906010791 1102 88-89 -0.8611005374127778 1102 90-91 -0.9843731807425904 1102 92-93 -1.033580194592183 1102 94-95 -0.8219809691900686 1102 96-97 -0.9337433901087593 1102 98-99 -0.6424196416133654 1102 100-101 -1.1126267657074536 1103 1 0.10191210152616037 1103 2 0.10700879074427405 1103 3 0.10810862833665169 1103 4 0.06727220497968744 1103 5 0.056636685033311096 1103 6 0.0750229829574991 1103 7 0.022018451804441952 1103 8 0.039135522347748974 1103 9 0.051405772426235785 1103 10-11 0.1315955570007148 1103 12-13 0.19536654566793032 1103 14-15 0.2315463625470997 1103 16-17 0.1874223028966 1103 18-19 0.1787116691609043 1103 20-21 0.19284529672018635 1103 22-23 0.2044000197052398 1103 24-25 0.13977942613646377 1103 26-27 0.1545353964951559 1103 28-29 0.14870756620354797 1103 30-31 0.3031139245640375 1103 32-33 0.24763266433511433 1103 34-35 0.2427966407037374 1103 36-37 0.3058084367819589 1103 38-39 0.2976985405506909 1103 40-41 0.30301408667488516 1103 42-43 0.291779322691724 1103 44-45 0.268259627655695 1103 46-47 0.24879143115209956 1103 48-49 0.3074035052393924 1103 50-51 0.19512313566225004 1103 52-53 0.13422997485127297 1103 54-55 0.1547246771705275 1103 56-57 0.18319141223027913 1103 58-59 0.38091503675003224 1103 60-61 0.25539139943214195 1103 62-63 0.2019175490626921 1103 64-65 0.23602702936948816 1103 66-67 0.21907262221773038 1103 68-69 0.24226907622564653 1103 70-71 -0.0023449875454453206 1103 72-73 -0.12345712489108962 1103 74-75 -0.16544024783763334 1103 76-77 -0.13878800650298118 1103 78-79 -0.17261663379311187 1103 80-81 -0.07928655573691401 1103 82-83 -0.13163400536264191 1103 84-85 -0.2012395817798307 1103 86-87 -0.3616974891825713 1103 88-89 -0.5854635375221342 1103 90-91 -0.8843729854636528 1103 92-93 -0.6944869137498237 1103 94-95 -0.9209110358973511 1103 96-97 -1.0451724804213605 1103 98-99 -0.8818876627347372 1103 100-101 -1.6338885410273214 1104 1 0.1695050704349157 1104 2 0.15220511488796262 1104 3 0.18174485855182354 1104 4 0.10937182339509377 1104 5 0.07678615781436093 1104 6 0.09844527743242537 1104 7 0.14828061622450406 1104 8 0.03224442071979894 1104 9 0.17910000801520454 1104 10-11 0.11345476583360181 1104 12-13 0.13880425028984433 1104 14-15 0.1689622558840469 1104 16-17 0.14657666402464287 1104 18-19 0.14512319346716396 1104 20-21 0.24947024873987544 1104 22-23 0.15142326475480417 1104 24-25 0.16835791063601135 1104 26-27 0.22379892694289794 1104 28-29 0.07373494320460594 1104 30-31 0.041557161033594525 1104 32-33 0.07132393078953925 1104 34-35 0.05927223283722327 1104 36-37 0.07220302686076252 1104 38-39 0.15878763716609967 1104 40-41 0.07045972780473875 1104 42-43 0.1096107819705523 1104 44-45 0.16858504706006272 1104 46-47 0.18004693477602984 1104 48-49 0.17308488210498751 1104 50-51 0.22921653357143867 1104 52-53 0.1712854513804345 1104 54-55 0.14230991278207483 1104 56-57 0.09049994256512406 1104 58-59 0.15789464794017505 1104 60-61 -0.07822163089142009 1104 62-63 -0.007473044538421902 1104 64-65 -0.017706403311315455 1104 66-67 -0.10208556604572294 1104 68-69 -0.10819040581278472 1104 70-71 -0.13038411690036256 1104 72-73 -0.47894437906255405 1104 74-75 -0.46284253734518543 1104 76-77 -0.5499185919756719 1104 78-79 -0.3438400188322852 1104 80-81 -0.2173648596046931 1104 82-83 -0.0814979670687066 1104 84-85 -0.16334783665124064 1104 86-87 -0.5082321817698947 1104 88-89 -0.6698174402970913 1104 90-91 -0.8615713304251535 1104 92-93 -0.8473589587271348 1104 94-95 -0.7920158813443017 1104 96-97 -0.8954104063171826 1104 98-99 -0.6921698434116514 1104 100-101 -1.2488095710799278 1105 1 0.14675405270271824 1105 2 0.21191314699248665 1105 3 0.10515401784554967 1105 4 0.08245083536996844 1105 5 0.017859692363430213 1105 6 0.12317174256039465 1105 7 0.08784699744709457 1105 8 0.11841968004436865 1105 9 0.16792715744461617 1105 10-11 0.1279153637320931 1105 12-13 0.12666951293553552 1105 14-15 0.1709344736655467 1105 16-17 0.12668439319134706 1105 18-19 0.15660904748752813 1105 20-21 0.168876301144671 1105 22-23 0.19771843965725822 1105 24-25 0.14880367276307283 1105 26-27 0.22523610529350435 1105 28-29 0.09422227046734832 1105 30-31 0.14724790285983858 1105 32-33 0.2252177421488426 1105 34-35 0.33837738208038814 1105 36-37 0.2680869372992447 1105 38-39 0.20316146071181862 1105 40-41 0.2073461212783272 1105 42-43 0.22489886087218025 1105 44-45 0.13827134924322593 1105 46-47 0.16679259780777045 1105 48-49 0.23651073850449222 1105 50-51 0.28813008056528844 1105 52-53 0.22921665736684105 1105 54-55 0.2808536366457517 1105 56-57 0.2749728183805402 1105 58-59 0.3651244624472767 1105 60-61 0.07471083195321171 1105 62-63 0.143492314080639 1105 64-65 0.26387047738727887 1105 66-67 0.20812642045233787 1105 68-69 0.24239014426588312 1105 70-71 0.1918762147702573 1105 72-73 0.16513221231642206 1105 74-75 -0.044067336261331747 1105 76-77 -0.02838083267128866 1105 78-79 0.10194229332392624 1105 80-81 0.020773978283202155 1105 82-83 -5.398804644443089E-4 1105 84-85 -0.22865736557432115 1105 86-87 -0.3341352492037011 1105 88-89 -0.45156118439968296 1105 90-91 -0.5381709449168675 1105 92-93 -0.34479948941735117 1105 94-95 -0.3195795626093627 1105 96-97 -0.6494106872936207 1105 98-99 -0.8279207583344608 1105 100-101 -1.5999892146058734 1106 1 0.19038510442175038 1106 2 0.15826922337925708 1106 3 0.09222121563012564 1106 4 0.08025977098207449 1106 5 0.13908430894746004 1106 6 0.08330947588885351 1106 7 0.10877113448201214 1106 8 0.13784954040259123 1106 9 0.13289103899069943 1106 10-11 0.1922985888521822 1106 12-13 0.15555859131603 1106 14-15 0.2291894386840454 1106 16-17 0.20200882989370683 1106 18-19 0.2109250910651852 1106 20-21 0.37162020729760314 1106 22-23 0.3008976351029631 1106 24-25 0.2696231495226087 1106 26-27 0.2702290602662387 1106 28-29 0.2335206304174804 1106 30-31 0.1898218959488176 1106 32-33 0.1827949024546882 1106 34-35 0.23452505153664305 1106 36-37 0.13335485136209968 1106 38-39 0.13760230481572222 1106 40-41 0.2090394656563177 1106 42-43 0.11808020840114608 1106 44-45 0.06729939941519092 1106 46-47 0.010114033775167286 1106 48-49 -0.020834869824476243 1106 50-51 -0.15099124008293785 1106 52-53 -0.21449255964029845 1106 54-55 -0.16649615403370177 1106 56-57 -0.1757316843461112 1106 58-59 -0.008430900909644379 1106 60-61 -0.16298920012501128 1106 62-63 -0.20485301813198475 1106 64-65 -0.26644699549850515 1106 66-67 -0.41076897205922336 1106 68-69 -0.558348365436764 1106 70-71 -0.9874199321886046 1106 72-73 -0.9535925362349857 1106 74-75 -0.6608967288459802 1106 76-77 -0.712350857805994 1106 78-79 -0.5676834878213874 1106 80-81 -0.4831146940438451 1106 82-83 -0.46276158158475056 1106 84-85 -0.5227286807408227 1106 86-87 -0.47629466172557855 1106 88-89 -0.7394577463451633 1106 90-91 -1.027706744618822 1106 92-93 -1.0532929093212822 1106 94-95 -1.1759463791097495 1106 96-97 -1.330832930089219 1106 98-99 -1.5338017318882002 1106 100-101 -1.7771371358152628 1107 1 -0.01079789785391938 1107 2 0.0030660717559882755 1107 3 0.05199875707427282 1107 4 0.048702370499498215 1107 5 -0.004884609368772885 1107 6 0.05495706433290337 1107 7 0.03207000884781763 1107 8 0.02681142588751584 1107 9 0.01986505372724423 1107 10-11 0.042379399705232856 1107 12-13 0.029361389963590057 1107 14-15 0.04794775324410239 1107 16-17 0.002122517803719859 1107 18-19 0.050527840921283484 1107 20-21 0.043808504208868726 1107 22-23 0.056209423957916727 1107 24-25 0.11185415801917742 1107 26-27 -0.034987623363086584 1107 28-29 -0.015528222030290806 1107 30-31 -0.07570093502308595 1107 32-33 0.012885128534477985 1107 34-35 0.08327124446874024 1107 36-37 0.08201470628374352 1107 38-39 0.018446892585068042 1107 40-41 -0.09229515226463292 1107 42-43 -0.06315966641827231 1107 44-45 -0.14642405593490793 1107 46-47 -0.22901785944777941 1107 48-49 -0.15336018992312717 1107 50-51 -0.24109982013714415 1107 52-53 -0.14726631593458706 1107 54-55 -0.13192292682178675 1107 56-57 -0.16573534370549226 1107 58-59 -0.16568157846290887 1107 60-61 -0.20064743882959135 1107 62-63 -0.07094009563797954 1107 64-65 -0.2786796430831444 1107 66-67 -0.5090424977376315 1107 68-69 -0.19894142942600013 1107 70-71 -0.4449157105838957 1107 72-73 -0.5345181254490221 1107 74-75 -0.5117826940591961 1107 76-77 -0.44843118836812934 1107 78-79 -0.4176068699842972 1107 80-81 -0.3349526644716505 1107 82-83 -0.3275698845187165 1107 84-85 -0.3938772049897956 1107 86-87 -0.7535181037342618 1107 88-89 -0.625937366381681 1107 90-91 -0.6975347547278012 1107 92-93 -0.804355076500638 1107 94-95 -0.6726036877271611 1107 96-97 -0.8227888195613211 1107 98-99 -1.0819576625280938 1107 100-101 -1.4443784362601022 1108 1 0.022599236029080316 1108 2 -0.038491534142018224 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6308.0 14 8200.0 15 11373.0 16 15390.0 17 21205.0 18 21914.0 19 17128.0 20 16527.0 21 17928.0 22 19399.0 23 21523.0 24 24435.0 25 27873.0 26 32759.0 27 38406.0 28 46377.0 29 56458.0 30 67699.0 31 82893.0 32 104253.0 33 134946.0 34 182707.0 35 259078.0 36 373401.0 37 483742.0 38 272364.0 39 12652.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.00730202298514 18.976082469906654 14.535782007899176 23.480833499209037 2 15.84021738401604 19.845217819856913 39.678533831309466 24.636030964817586 3 19.46783242454059 23.51649991032202 29.473883592952483 27.541784072184903 4 13.216131484183366 16.540444778168208 36.06852268851365 34.17490104913477 5 14.24658969250141 36.81376400315941 33.92360827398948 15.016038030349693 6 33.03364722436274 35.42116332012988 16.93976569004696 14.60542376546042 7 29.500603593233887 32.15947264262378 19.88342123600642 18.45650252813591 8 26.602685644202218 35.29011234250742 19.108324558429963 18.998877454860402 9 26.374698779530707 16.711969331346076 20.022869919268096 36.89046196985512 10-11 25.53071579327571 26.42843764259655 26.588627641427486 21.452218922700258 12-13 25.882116564359336 23.59501927179697 27.512022219815186 23.01084194402851 14-15 22.772183675034285 25.07407163757086 25.636706010917052 26.5170386764778 16-17 22.634591532298614 28.530010727095974 26.173548489826022 22.66184925077939 18-19 22.46747489358029 26.812717652870827 28.605312039474708 22.114495414074177 20-21 23.004549261881525 26.122031844414156 28.83329890414621 22.04011998955811 22-23 22.867237562648203 25.854804086751493 28.72632399778592 22.551634352814386 24-25 22.659460995154525 26.109496790949414 28.777891544922202 22.453150668973855 26-27 22.45633181367458 26.544825742164285 28.600032186925574 22.398810257235564 28-29 22.268396345506943 26.52252210725252 28.897230216091124 22.31185133114941 30-31 22.43536881344114 26.255120546549204 28.786082672942058 22.523427967067594 32-33 22.290880399708197 26.35362225078052 28.6712023803488 22.684294969162483 34-35 22.81947312664271 26.384693651772785 28.41500915222872 22.38082406935579 36-37 22.7693575711255 26.111209580808215 28.415379542019537 22.704053306046752 38-39 22.386873282236753 26.37788759171007 28.60459483729254 22.63064428876064 40-41 22.64510831727589 26.226652634869406 28.57190147264732 22.55633757520738 42-43 22.612398981920457 26.15202304339409 28.443162952229546 22.792415022455913 44-45 22.56500097840347 26.45975912418852 28.276258400756575 22.69898149665143 46-47 22.539178654581086 26.73004445146531 27.86281620365589 22.867960690297714 48-49 22.70808641764506 26.566434523144828 27.939517956326902 22.785961102883206 50-51 22.66198438134771 26.739729425590365 27.73703021842354 22.86125597463839 52-53 22.832731403035723 26.800882770573725 27.572542256470705 22.79384356991984 54-55 22.660092638978895 26.644612161490272 27.667131600611057 23.028163598919775 56-57 22.599223436868098 26.992253227472652 27.729883431713365 22.67863990394589 58-59 22.807086293237617 26.624920132616005 27.634272029619616 22.93372154452676 60-61 22.75489361505985 26.654113859007822 27.77546495288088 22.815527573051455 62-63 22.581492823552043 26.759368651954325 27.702132670932816 22.957005853560815 64-65 22.464738735129163 26.577861449443972 27.700911885185786 23.256487930241075 66-67 22.413423886441507 26.815986261138935 27.599462581886698 23.17112727053286 68-69 22.387089716269845 26.629000565012483 27.752968830424162 23.23094088829351 70-71 22.346339098136035 26.73600997759149 27.637609920931105 23.28004100334137 72-73 22.703676117024788 26.617534442317826 27.347477966449706 23.331311474207677 74-75 22.457496864716035 26.890730769996406 27.27723244267171 23.37453992261585 76-77 22.426279910755728 26.812949765416317 27.45797369737374 23.302796626454217 78-79 22.476067931866954 26.838284603004293 27.379208020386265 23.306439444742487 80-81 22.266582630071635 27.045500452171638 27.459180928162958 23.228735989593766 82-83 22.508927656379907 26.970272303212738 27.245264449138606 23.275535591268742 84-85 22.493034912924625 26.67942158605956 27.279461611080436 23.548081889935386 86-87 22.44490145521815 26.596077992500188 27.473920102473244 23.485100449808417 88-89 22.412937903720117 26.745962143953562 27.26565766105584 23.575442291270477 90-91 22.424344794474642 26.779919388014495 27.375361686191663 23.4203741313192 92-93 22.435461796363366 26.47153432953174 27.354759834624947 23.73824403947995 94-95 22.253785676254513 26.54347490370143 27.33492205279653 23.86781736724753 96-97 22.448419822746573 26.461054200255806 27.259042456000955 23.83148352099667 98-99 22.30870756905796 26.47930906523956 27.012588032097735 24.19939533360475 100-101 22.70086529554504 26.000625656221395 26.984179865477625 24.31432918275594 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 850.0 1 570.5 2 387.5 3 557.0 4 486.5 5 435.5 6 961.5 7 2026.5 8 3193.0 9 3713.5 10 3161.5 11 2510.0 12 2474.5 13 2647.0 14 2805.5 15 2856.5 16 2904.5 17 3029.5 18 3147.0 19 3333.0 20 3639.0 21 4159.0 22 4948.5 23 5994.5 24 7168.5 25 8798.5 26 11329.5 27 14195.5 28 16597.0 29 19413.0 30 23198.5 31 26744.5 32 30812.0 33 34984.0 34 38716.0 35 42516.5 36 46978.0 37 51970.0 38 56543.0 39 59249.5 40 62330.0 41 68964.0 42 75086.0 43 80662.0 44 86794.5 45 91092.0 46 92295.5 47 93911.0 48 97889.0 49 99032.5 50 97150.5 51 93747.0 52 89657.5 53 87338.0 54 86315.5 55 80936.0 56 71958.5 57 65764.0 58 60496.5 59 53400.0 60 44746.5 61 35987.0 62 28610.5 63 22394.5 64 17706.5 65 13758.0 66 9926.5 67 7848.5 68 5980.0 69 3862.5 70 2776.5 71 2122.0 72 1712.5 73 1396.5 74 1143.5 75 911.0 76 589.5 77 314.0 78 160.5 79 88.0 80 43.5 81 21.5 82 16.5 83 12.0 84 4.5 85 3.0 86 2.5 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009050755884772145 2 0.014623675017525366 3 0.009427870713304318 4 1.2570494284405758E-4 5 0.0010894428379818323 6 0.0018436724950461776 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 9.427870713304318E-4 14-15 0.0 16-17 0.0025560005044958376 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0036663941662850126 26-27 0.020070889207434526 28-29 0.006850919385001138 30-31 0.0032264268663308106 32-33 0.0 34-35 0.009930690484680548 36-37 8.380329522937171E-4 38-39 0.0033521318091748686 40-41 0.002367443090229751 42-43 0.0036454433424776694 44-45 0.0 46-47 2.304590618807722E-4 48-49 6.075738904129449E-4 50-51 0.0041692139376612435 52-53 0.007982263870597656 54-55 0.007563247394450797 56-57 0.01617403597926874 58-59 0.023234463602343308 60-61 0.0037501974615143843 62-63 0.019777577674131727 64-65 0.0207413155692695 66-67 0.013282822293855415 68-69 0.03155194065385845 70-71 0.01650924916018623 72-73 0.005174853480413703 74-75 0.016488298336378885 76-77 0.0211812828692237 78-79 0.026020923168719918 80-81 0.011690559684497354 82-83 0.03505072822968472 84-85 0.022354529002434904 86-87 0.03132148159197767 88-89 0.022145020764361475 90-91 0.027969349782802813 92-93 0.02390488996417828 94-95 0.014351314308029906 96-97 0.014791281607984106 98-99 0.011166789089313782 100-101 0.010328756137020063 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2386541.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.05277594566624 #Duplication Level Percentage of deduplicated Percentage of total 1 75.10257772921739 38.34195073751719 2 13.700863003171241 13.989341783263384 3 4.603685432918158 7.050927627932947 4 2.0704748111220788 4.228139465334444 5 1.130508472932058 2.8857797886638825 6 0.6961530840829783 2.1324328455343706 7 0.4608158340035342 1.6468149267918433 8 0.3216902204479984 1.3138542998754914 9 0.24948229362356933 1.1463087274897503 >10 1.461389169525448 13.800353897965161 >50 0.12634238639710754 4.482381857713942 >100 0.07240123571784553 6.679542618175281 >500 0.002547866637696952 0.8094063129077212 >1k 9.040817101503242E-4 0.7807057469133307 >5k 1.6437849275460442E-4 0.7120593639212739 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9157 0.38369338720767837 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7813 0.32737757281354063 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4909 0.20569518814049287 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 8.380329522937171E-5 0.0 0.0 0.0 0.0 8 8.380329522937171E-5 0.0 0.0 0.0 0.0 9 8.380329522937171E-5 0.0 0.0 4.1901647614685854E-5 0.0 10-11 8.380329522937171E-5 0.0 0.0 4.1901647614685854E-5 0.0 12-13 8.380329522937171E-5 0.0 0.0 4.1901647614685854E-5 0.0 14-15 1.2570494284405758E-4 0.0 0.0 4.1901647614685854E-5 0.0 16-17 1.2570494284405758E-4 0.0 0.0 8.380329522937171E-5 0.0 18-19 1.2570494284405758E-4 0.0 0.0 1.2570494284405758E-4 0.0 20-21 1.2570494284405758E-4 0.0 0.0 1.6760659045874342E-4 0.0 22-23 1.2570494284405758E-4 0.0 0.0 2.93311533302801E-4 0.0 24-25 1.2570494284405758E-4 0.0 0.0 5.656722427982591E-4 0.0 26-27 1.2570494284405758E-4 0.0 0.0 0.0013618035474772905 0.0 28-29 1.466557666514005E-4 0.0 0.0 0.00450442711857873 0.0 30-31 1.6760659045874342E-4 0.0 0.0 0.013974199479497733 0.0 32-33 1.6760659045874342E-4 0.0 0.0 0.03295564584895043 0.0 34-35 1.6760659045874342E-4 0.0 0.0 0.062223946707808495 0.0 36-37 1.6760659045874342E-4 0.0 0.0 0.1143914979880924 0.0 38-39 1.6760659045874342E-4 0.0 0.0 0.19186764442764653 0.0 40-41 1.6760659045874342E-4 0.0 0.0 0.26984661063857696 0.0 42-43 1.8855741426608634E-4 0.0 0.0 0.34937593781125065 0.0 44-45 2.0950823807342928E-4 0.0 0.0 0.4448278910775051 0.0 46-47 3.3521318091748683E-4 0.0 0.0 0.5568519459753677 0.0 48-49 3.7711482853217273E-4 0.0 0.0 0.6745327233012129 0.0 50-51 3.7711482853217273E-4 0.0 0.0 0.794832353602976 0.0 52-53 3.7711482853217273E-4 0.0 0.0 0.9160328693284548 2.0950823807342927E-5 54-55 3.7711482853217273E-4 0.0 0.0 1.0447966324483846 4.1901647614685854E-5 56-57 3.7711482853217273E-4 0.0 0.0 1.1932541699472166 4.1901647614685854E-5 58-59 3.7711482853217273E-4 0.0 0.0 1.3532137097162797 4.1901647614685854E-5 60-61 3.7711482853217273E-4 0.0 0.0 1.5166301354135545 4.1901647614685854E-5 62-63 3.7711482853217273E-4 0.0 0.0 1.6825606599677108 4.1901647614685854E-5 64-65 3.7711482853217273E-4 0.0 0.0 1.8541688577736566 4.1901647614685854E-5 66-67 3.7711482853217273E-4 0.0 0.0 2.044863256068092 4.1901647614685854E-5 68-69 3.7711482853217273E-4 0.0 0.0 2.2515012312799154 4.1901647614685854E-5 70-71 3.9806565233951565E-4 0.0 0.0 2.4707306515999514 4.1901647614685854E-5 72-73 4.1901647614685857E-4 0.0 0.0 2.6887030224915476 4.1901647614685854E-5 74-75 4.6091812376154446E-4 0.0 0.0 2.905502147249932 4.1901647614685854E-5 76-77 5.028197713762303E-4 0.0 0.0 3.1456823913773113 4.1901647614685854E-5 78-79 5.028197713762303E-4 0.0 0.0 3.4087409351023092 4.1901647614685854E-5 80-81 6.285247142202878E-4 0.0 0.0 3.6892305642350163 4.1901647614685854E-5 82-83 6.285247142202878E-4 0.0 0.0 3.967708914282219 4.1901647614685854E-5 84-85 6.285247142202878E-4 0.0 0.0 4.252158249114514 4.1901647614685854E-5 86-87 6.285247142202878E-4 0.0 0.0 4.542662372027131 4.1901647614685854E-5 88-89 6.285247142202878E-4 0.0 0.0 4.857490401380073 4.1901647614685854E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4200 0.0 60.622288 1 GTATCAA 7270 0.0 42.86336 1 ATCAACG 9290 0.0 33.228775 3 TCAACGC 9435 0.0 32.66503 4 TATCAAC 9580 0.0 32.426624 2 CAACGCA 9640 0.0 32.019653 5 AACGCAG 10010 0.0 30.883549 6 ACGCAGA 11615 0.0 26.493301 7 CGCAGAG 11655 0.0 26.239399 8 GTGGTAT 2025 0.0 23.692623 1 GCAGAGT 12980 0.0 23.23161 9 GAGTACT 7665 0.0 23.078768 12-13 TGGTATC 1860 0.0 22.983652 2 GTACATG 8455 0.0 22.810207 1 TACATGG 8370 0.0 22.586405 2 CAGAGTA 12630 0.0 21.563738 10-11 GTACTTT 8105 0.0 21.56221 14-15 ACATGGG 8645 0.0 20.985292 3 AGTACTT 7925 0.0 19.565113 12-13 AGAGTAC 12710 0.0 19.167515 10-11 >>END_MODULE