FastQCFastQC Report
Thu 26 May 2016
SRR938151_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938151_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1985460
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90890.4577780463973084No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73070.36802554571736523No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46550.23445448409940267No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA25610.12898774087616974No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA23930.12052622566055221No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA23080.11624510189074573No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA22470.1131727660088846No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC21520.10838798061910085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40050.055.752471
GTATCAA74500.042.587891
TATCAAC95300.033.115292
ATCAACG95600.032.9616553
TCAACGC96150.032.7731134
CAACGCA98850.031.9741045
AACGCAG102300.031.0769376
ACGCAGA115950.027.2900057
CGCAGAG115550.027.1788868
TGGTATC17950.026.2134482
GCAGAGT125750.024.7445039
GTGGTAT19600.024.5858971
GAGTACT77950.023.95029812-13
TACATGG70100.022.78112
GTACATG72450.022.719641
GTACTTT82800.022.2608314-15
AGAGTAC112600.021.9803110-11
AGTACTT81150.021.80581312-13
CAGAGTA119300.021.7612710-11
ACATGGG73100.021.1959883
Produced by FastQC (version 0.11.5)