##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938151_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1985460 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.861136965740936 33.0 31.0 34.0 30.0 34.0 2 32.023420265328944 33.0 31.0 34.0 30.0 34.0 3 32.110398597806046 34.0 31.0 34.0 30.0 34.0 4 35.64448037230667 37.0 35.0 37.0 33.0 37.0 5 35.656764679217915 37.0 35.0 37.0 33.0 37.0 6 35.69021133641574 37.0 35.0 37.0 33.0 37.0 7 35.62212434398074 37.0 35.0 37.0 33.0 37.0 8 35.59282382923856 37.0 35.0 37.0 33.0 37.0 9 37.21726703131768 39.0 37.0 39.0 34.0 39.0 10-11 37.11608871495774 39.0 37.0 39.0 33.5 39.0 12-13 36.97676810411693 39.0 37.0 39.0 33.0 39.0 14-15 38.12352678976157 40.0 38.0 41.0 33.0 41.0 16-17 37.94021209190817 40.0 38.0 41.0 33.0 41.0 18-19 38.133146978533944 40.0 38.0 41.0 33.0 41.0 20-21 38.12240891279603 40.0 38.0 41.0 33.0 41.0 22-23 38.00727891773191 40.0 38.0 41.0 33.0 41.0 24-25 37.86225484270648 40.0 38.0 41.0 33.0 41.0 26-27 37.652745207659684 40.0 37.5 41.0 32.0 41.0 28-29 37.43449326604414 40.0 37.0 41.0 32.0 41.0 30-31 37.16621085290059 40.0 37.0 41.0 31.0 41.0 32-33 36.831408338621785 39.0 36.0 41.0 30.5 41.0 34-35 36.52167482598491 39.0 35.5 41.0 30.0 41.0 36-37 36.19815030270064 39.0 35.0 40.5 29.5 41.0 38-39 35.84776575705378 38.5 35.0 40.0 28.5 41.0 40-41 35.660445186505896 39.0 35.0 40.0 27.5 41.0 42-43 35.37319915787777 38.0 35.0 40.0 26.5 41.0 44-45 34.986646671300356 38.0 34.0 40.0 25.0 41.0 46-47 34.50092623371914 38.0 33.5 40.0 23.5 41.0 48-49 33.98778796853122 38.0 33.0 40.0 22.0 41.0 50-51 33.143388685745364 37.5 32.0 39.5 19.0 40.5 52-53 33.095469060066684 37.5 32.5 39.5 18.5 40.5 54-55 33.03809192831888 37.5 32.5 40.0 14.0 41.0 56-57 32.52824358083265 37.0 31.5 40.0 8.5 41.0 58-59 32.028327944154 36.5 31.0 40.0 6.5 41.0 60-61 31.427729090487844 36.0 30.5 39.0 2.0 41.0 62-63 30.774580449870562 35.0 29.0 39.0 2.0 40.5 64-65 30.133952585295095 35.0 28.0 38.5 2.0 40.0 66-67 29.417117443816544 34.5 27.5 38.0 2.0 40.0 68-69 28.67237416014425 34.0 26.0 37.5 2.0 40.0 70-71 27.79847289796823 34.0 24.5 37.0 2.0 39.0 72-73 26.97746794193789 33.5 22.5 36.0 2.0 39.0 74-75 26.118887562579957 32.5 19.0 35.5 2.0 38.5 76-77 25.249443705740735 32.0 14.0 35.0 2.0 37.5 78-79 24.393021264593592 31.0 7.0 35.0 2.0 37.0 80-81 23.56016238050628 31.0 2.0 35.0 2.0 36.0 82-83 22.713771367844227 30.0 2.0 34.0 2.0 36.0 84-85 21.885032435808327 29.5 2.0 34.0 2.0 35.0 86-87 21.094873983862684 29.0 2.0 34.0 2.0 35.0 88-89 20.242053478790808 27.5 2.0 34.0 2.0 35.0 90-91 19.381601492853044 26.0 2.0 34.0 2.0 35.0 92-93 18.463929517592902 24.5 2.0 33.0 2.0 35.0 94-95 17.51235456770723 21.5 2.0 33.0 2.0 35.0 96-97 16.409017809474882 9.5 2.0 32.0 2.0 34.5 98-99 15.10637232681595 2.0 2.0 32.0 2.0 34.0 100-101 12.169817070099624 2.0 2.0 27.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5410751908591642 1101 2 0.5989128154134988 1101 3 0.560533437127809 1101 4 0.38625924016093904 1101 5 0.41625275379541904 1101 6 0.3188795792802992 1101 7 0.40367322689107255 1101 8 0.32917203618000457 1101 9 0.4297266039818055 1101 10-11 0.5366665114681695 1101 12-13 0.5816486307347191 1101 14-15 0.6690375567254279 1101 16-17 0.7850730589798758 1101 18-19 0.7672613139149291 1101 20-21 0.672582362569905 1101 22-23 0.7473762258652599 1101 24-25 0.8351417996515522 1101 26-27 0.9711790278617229 1101 28-29 1.1659940852064494 1101 30-31 1.0761229385297284 1101 32-33 1.3034175317373666 1101 34-35 1.3509835270848853 1101 36-37 1.3509060898847238 1101 38-39 1.6980400674763203 1101 40-41 1.8907499155608036 1101 42-43 1.9314632357832267 1101 44-45 2.0414720752263875 1101 46-47 2.1807264301141487 1101 48-49 2.399146941466263 1101 50-51 2.442201569155081 1101 52-53 2.720887416593442 1101 54-55 2.8851707866524237 1101 56-57 3.1889554795375616 1101 58-59 3.377517838332693 1101 60-61 3.6052794950218043 1101 62-63 3.7000376765702754 1101 64-65 3.6614128612555668 1101 66-67 3.8906098013182664 1101 68-69 4.173743127680499 1101 70-71 4.412469121348682 1101 72-73 4.838654271603488 1101 74-75 5.136166728053976 1101 76-77 5.2326286099223935 1101 78-79 5.451949768969236 1101 80-81 5.52481268005619 1101 82-83 6.10097621311855 1101 84-85 6.1609818588481104 1101 86-87 6.308715450565128 1101 88-89 6.282630408247584 1101 90-91 6.402199680097542 1101 92-93 6.572799478858684 1101 94-95 6.684374586564214 1101 96-97 6.790032172581206 1101 98-99 6.62773900810787 1101 100-101 5.503723890646343 1102 1 0.6117004237214196 1102 2 0.4753148767747817 1102 3 0.41834082453313925 1102 4 0.30128563429114763 1102 5 0.2624629550990747 1102 6 0.3679464702325106 1102 7 0.4802128844867113 1102 8 0.4607398585812561 1102 9 0.5801993374250642 1102 10-11 0.5316407444521616 1102 12-13 0.6404643808924249 1102 14-15 0.8251069155564821 1102 16-17 0.7801378546328692 1102 18-19 0.7055663717754399 1102 20-21 0.6713943500423767 1102 22-23 0.7885219266284977 1102 24-25 0.7829491132787396 1102 26-27 0.7938259662532943 1102 28-29 0.8536399353704169 1102 30-31 0.9988202305030356 1102 32-33 1.1430205315566084 1102 34-35 1.112850003403942 1102 36-37 1.247160004737367 1102 38-39 1.4315574772733157 1102 40-41 1.4951622173281578 1102 42-43 1.6051688905088994 1102 44-45 1.5471273542368706 1102 46-47 1.6537382014166582 1102 48-49 2.099315340074803 1102 50-51 2.103904442444815 1102 52-53 2.217152582612705 1102 54-55 2.2785432961790235 1102 56-57 2.598095300182898 1102 58-59 2.855745539811572 1102 60-61 2.9223998972602345 1102 62-63 3.2793003009325616 1102 64-65 3.4158327252824527 1102 66-67 3.525715101171077 1102 68-69 3.753649097438842 1102 70-71 3.8911180960169602 1102 72-73 4.239080155926743 1102 74-75 4.509200079462953 1102 76-77 4.731683327481225 1102 78-79 5.007841322478679 1102 80-81 5.273533735968957 1102 82-83 5.380529523359325 1102 84-85 5.388104548208936 1102 86-87 5.535584896454399 1102 88-89 5.510495490159769 1102 90-91 5.752612284355276 1102 92-93 5.8314210080582285 1102 94-95 5.7920279620986435 1102 96-97 5.855312916855098 1102 98-99 5.918954060324349 1102 100-101 4.839161561027739 1103 1 0.4110122858188383 1103 2 0.3144045680398264 1103 3 0.26181636192168867 1103 4 0.17559126363281052 1103 5 0.1901178486283328 1103 6 0.1969050687617937 1103 7 0.2235545999216555 1103 8 0.25033105230502883 1103 9 0.2202787000242239 1103 10-11 0.2737334781353624 1103 12-13 0.316653621940695 1103 14-15 0.6170445074418893 1103 16-17 0.6587527661624648 1103 18-19 0.5697167759844035 1103 20-21 0.6048641095448133 1103 22-23 0.6523447205280988 1103 24-25 0.705580127888048 1103 26-27 0.8431036263205272 1103 28-29 0.8605803432808443 1103 30-31 0.9350344859092061 1103 32-33 0.9541471985149528 1103 34-35 1.0502979624976163 1103 36-37 1.0376442707617954 1103 38-39 0.9822514833414857 1103 40-41 1.1650306801396155 1103 42-43 1.160366279626615 1103 44-45 1.3108244860580953 1103 46-47 1.525870699734341 1103 48-49 1.5582713413331604 1103 50-51 1.7509058098315649 1103 52-53 1.8107069409235876 1103 54-55 1.9445957152174316 1103 56-57 2.084066738536258 1103 58-59 2.33725491533459 1103 60-61 2.4574642699950004 1103 62-63 2.497091016452927 1103 64-65 2.5124921765360533 1103 66-67 2.861761158979103 1103 68-69 3.0330302038901387 1103 70-71 3.1475871409226635 1103 72-73 3.273572642938653 1103 74-75 3.463027338724064 1103 76-77 3.8697124780277967 1103 78-79 3.991364451037338 1103 80-81 4.159119958766187 1103 82-83 4.367363477884862 1103 84-85 4.6331372675724225 1103 86-87 4.796056273348267 1103 88-89 4.711008451166677 1103 90-91 4.994935230688299 1103 92-93 4.998609559510236 1103 94-95 4.886307160389169 1103 96-97 5.197832186313626 1103 98-99 5.137278813635696 1103 100-101 4.0327188360599795 1104 1 0.6132627003839879 1104 2 0.520304303521435 1104 3 0.4796155995425906 1104 4 0.38316456085546235 1104 5 0.4219300577872076 1104 6 0.40425554319264023 1104 7 0.42905650586939714 1104 8 0.4728439961096953 1104 9 0.5120302401791861 1104 10-11 0.5553747927324508 1104 12-13 0.5984001318472281 1104 14-15 0.7830890873013843 1104 16-17 0.8440619543033137 1104 18-19 0.7949437587725399 1104 20-21 0.7658626616748307 1104 22-23 0.8985882916602108 1104 24-25 0.8502829702653045 1104 26-27 0.9248772001112116 1104 28-29 1.049076471137134 1104 30-31 1.0976764756689263 1104 32-33 1.0844268076474322 1104 34-35 1.121046111538334 1104 36-37 1.149262232000197 1104 38-39 1.3742254637598492 1104 40-41 1.4581765363051105 1104 42-43 1.5342409288958905 1104 44-45 1.6549795477694857 1104 46-47 1.749539357461316 1104 48-49 1.9304518679600093 1104 50-51 1.9549531515460927 1104 52-53 2.1361362812342435 1104 54-55 2.310617056476758 1104 56-57 2.625760421271032 1104 58-59 2.738684997750333 1104 60-61 2.7778165021438355 1104 62-63 3.1004994401107737 1104 64-65 3.3290812855700374 1104 66-67 3.3241112795711807 1104 68-69 3.528689941054875 1104 70-71 3.691649630761752 1104 72-73 4.091240960215355 1104 74-75 4.186125072817099 1104 76-77 4.461262521349138 1104 78-79 4.662064879660409 1104 80-81 4.934519366229697 1104 82-83 5.10054035710343 1104 84-85 5.101093819996983 1104 86-87 5.163067406837289 1104 88-89 5.440711016210848 1104 90-91 5.3407566817332714 1104 92-93 5.36959659753547 1104 94-95 5.462461392013939 1104 96-97 5.3188555645366655 1104 98-99 5.130271849706212 1104 100-101 4.235081140181533 1105 1 0.5447613924831209 1105 2 0.45375470352907143 1105 3 0.43855693971137555 1105 4 0.3450331645519569 1105 5 0.2766737044439438 1105 6 0.3704864020157146 1105 7 0.3926047359072058 1105 8 0.3770969412733294 1105 9 0.5363588669787447 1105 10-11 0.5314769617476145 1105 12-13 0.5005286331073933 1105 14-15 0.7340469814855268 1105 16-17 0.7659963229786086 1105 18-19 0.7042895816044847 1105 20-21 0.7106888755404839 1105 22-23 0.7090405198650487 1105 24-25 0.8328849650527914 1105 26-27 0.8615731871467602 1105 28-29 0.9388885081211313 1105 30-31 0.9597060609593413 1105 32-33 0.9954188432948925 1105 34-35 1.038907311752176 1105 36-37 1.1178093641650122 1105 38-39 1.1913750155412757 1105 40-41 1.2078776045832669 1105 42-43 1.3792325993812469 1105 44-45 1.4931375555596134 1105 46-47 1.580933214623272 1105 48-49 1.6345715170225148 1105 50-51 1.8945498502897493 1105 52-53 1.7464095680750447 1105 54-55 1.7813071293182503 1105 56-57 2.062249960450778 1105 58-59 2.250391665180736 1105 60-61 2.5140155226501975 1105 62-63 2.535863957810392 1105 64-65 2.492970154569928 1105 66-67 2.7490480922072216 1105 68-69 2.960521276736703 1105 70-71 3.3465747247969624 1105 72-73 3.610645268650835 1105 74-75 3.8073538194280125 1105 76-77 4.020253504470535 1105 78-79 4.270603049840268 1105 80-81 4.549219348568261 1105 82-83 4.88831375134485 1105 84-85 4.87710445981827 1105 86-87 5.1263468369934735 1105 88-89 5.346450863272366 1105 90-91 5.5723291222571945 1105 92-93 5.654311460826417 1105 94-95 5.342091850352045 1105 96-97 5.34584504166272 1105 98-99 5.233739219011593 1105 100-101 3.8825165327427253 1106 1 0.42245996306962397 1106 2 0.33406434838613563 1106 3 0.36002669361647577 1106 4 0.23928695912902498 1106 5 0.3182620849331883 1106 6 0.3094416039019521 1106 7 0.23791124931716467 1106 8 0.3039269957973474 1106 9 0.2983780977479782 1106 10-11 0.3044838877717808 1106 12-13 0.43638232084685313 1106 14-15 0.5767700510404907 1106 16-17 0.49356272807735024 1106 18-19 0.5786748048582382 1106 20-21 0.6397454810518326 1106 22-23 0.6168378231364855 1106 24-25 0.6425459398538536 1106 26-27 0.7803420679307322 1106 28-29 0.6890381728720882 1106 30-31 0.8396334850678002 1106 32-33 0.7397246751546049 1106 34-35 0.7377910429635008 1106 36-37 0.779392034368378 1106 38-39 0.9378958857151574 1106 40-41 1.1551357463457208 1106 42-43 0.9006415663721086 1106 44-45 1.1600436858278087 1106 46-47 1.1838173695009573 1106 48-49 1.4304287967160079 1106 50-51 1.7372591046369266 1106 52-53 1.6167883810723893 1106 54-55 1.6696411073612722 1106 56-57 1.64694869681977 1106 58-59 1.9402770910575384 1106 60-61 2.2024365365022405 1106 62-63 2.1306961490865532 1106 64-65 2.402593367803046 1106 66-67 2.5437379868432366 1106 68-69 2.653600318145486 1106 70-71 2.9415289504085145 1106 72-73 3.1087617953233995 1106 74-75 3.262931018809091 1106 76-77 3.180302686610233 1106 78-79 3.6150829533180193 1106 80-81 3.7372788644833825 1106 82-83 3.792026358182458 1106 84-85 3.981330992385317 1106 86-87 3.917734896969897 1106 88-89 4.081977130587429 1106 90-91 4.1317897765454354 1106 92-93 4.280605485288543 1106 94-95 4.326630330310781 1106 96-97 4.256826750327619 1106 98-99 4.108352393193734 1106 100-101 3.1506927411129 1107 1 0.5061964571834743 1107 2 0.45784478702505993 1107 3 0.39543428916558554 1107 4 0.2581044885597592 1107 5 0.24039158017588846 1107 6 0.33948716107015287 1107 7 0.3203273918748195 1107 8 0.25607181114970956 1107 9 0.32324303888650263 1107 10-11 0.32155774682676963 1107 12-13 0.33634301267075273 1107 14-15 0.6528767273830169 1107 16-17 0.5146933924863148 1107 18-19 0.40764896225221037 1107 20-21 0.6193001899697492 1107 22-23 0.6239586504224093 1107 24-25 0.5276451174058607 1107 26-27 0.6354410439023468 1107 28-29 0.7901256990775423 1107 30-31 0.8509421449613583 1107 32-33 0.896614692893678 1107 34-35 0.9135842617991656 1107 36-37 1.092360708775722 1107 38-39 1.0402130523011976 1107 40-41 1.2254973815658445 1107 42-43 1.3091996618405872 1107 44-45 1.3451196816349338 1107 46-47 1.4560365680173248 1107 48-49 1.5925095095042607 1107 50-51 1.6160740135225922 1107 52-53 1.8377406468359752 1107 54-55 1.8888799398076515 1107 56-57 2.2089649038831496 1107 58-59 2.1769576667711377 1107 60-61 2.3195597827528474 1107 62-63 2.4501506725505067 1107 64-65 2.60304490787723 1107 66-67 2.700774150365252 1107 68-69 2.744296375233052 1107 70-71 3.208119016526883 1107 72-73 3.244642096887663 1107 74-75 3.375412876573968 1107 76-77 3.5125172487776233 1107 78-79 3.5027522808458365 1107 80-81 3.5529688741592445 1107 82-83 4.035424600401452 1107 84-85 3.935340426347583 1107 86-87 4.052672810209696 1107 88-89 4.258828646471962 1107 90-91 4.2523650869792355 1107 92-93 4.489805614299993 1107 94-95 4.3447423283756095 1107 96-97 4.427560301367773 1107 98-99 4.367070745072461 1107 100-101 3.481289620648459 1108 1 0.41698905180161105 1108 2 0.4121409617888361 1108 3 0.31996259105237357 1108 4 0.2779472052168046 1108 5 0.2559401476112484 1108 6 0.17641329967799635 1108 7 0.30228993960165695 1108 8 0.32933060453429164 1108 9 0.36165744454181237 1108 10-11 0.37244189594718335 1108 12-13 0.3687326965849138 1108 14-15 0.49904440175686915 1108 16-17 0.6018803196376865 1108 18-19 0.5426196931690725 1108 20-21 0.5566147262725281 1108 22-23 0.4257365699881319 1108 24-25 0.45464078274580544 1108 26-27 0.5652952555107333 1108 28-29 0.659264286667046 1108 30-31 0.8278142522340701 1108 32-33 0.7178049014232357 1108 34-35 0.8376416251510364 1108 36-37 0.9813989813934327 1108 38-39 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1.2618451089104354 2311 52-53 1.3195699744789806 2311 54-55 1.651203988924152 2311 56-57 2.073787829908902 2311 58-59 2.075691849909795 2311 60-61 2.1052562861969903 2311 62-63 2.228328551406456 2311 64-65 2.419555745702933 2311 66-67 2.414149693192442 2311 68-69 2.7980905501357185 2311 70-71 3.3410405499201126 2311 72-73 3.52554674648335 2311 74-75 3.7400200755856474 2311 76-77 3.580382814815362 2311 78-79 3.9343499710225345 2311 80-81 4.0490577106372285 2311 82-83 4.174028037059134 2311 84-85 4.431653413020239 2311 86-87 4.523437354234854 2311 88-89 4.47980985342015 2311 90-91 4.82185960255276 2311 92-93 4.81508732289538 2311 94-95 4.919924362667313 2311 96-97 5.0820442411701094 2311 98-99 4.8005341697505095 2311 100-101 3.855561483481642 2312 1 -0.3404006546578664 2312 2 -0.20453452606498956 2312 3 -0.17249582109176487 2312 4 -0.05166178306917146 2312 5 -0.22869376609293823 2312 6 -0.09380320950763377 2312 7 -0.08707116981081953 2312 8 -0.061269219791711294 2312 9 -0.028391787757179543 2312 10-11 -0.08383840272205134 2312 12-13 -0.10280028985201284 2312 14-15 0.0716247313764029 2312 16-17 0.016920455453515615 2312 18-19 -0.029768280682731074 2312 20-21 -0.07711189377423011 2312 22-23 -0.13406838454350378 2312 24-25 -0.04498973485990376 2312 26-27 -0.031244496179290593 2312 28-29 0.14613629838738973 2312 30-31 0.1227489461623037 2312 32-33 0.15191777934901296 2312 34-35 0.321739590233193 2312 36-37 0.26573876180795963 2312 38-39 0.43466056224577443 2312 40-41 0.5707990908338161 2312 42-43 0.5573548713148995 2312 44-45 0.7482253992959684 2312 46-47 0.7215820852037993 2312 48-49 0.9469760347003913 2312 50-51 1.0207092784870326 2312 52-53 1.0465343771103974 2312 54-55 1.1233964189297723 2312 56-57 1.4288850270155962 2312 58-59 1.471665126551315 2312 60-61 1.7664844226938428 2312 62-63 1.882648931620956 2312 64-65 1.9219551774856818 2312 66-67 2.099764891639783 2312 68-69 2.4106829146677384 2312 70-71 2.7997235953774933 2312 72-73 3.0780107749799512 2312 74-75 2.5858655228326377 2312 76-77 2.865006068852896 2312 78-79 3.205576178386753 2312 80-81 3.537669397673458 2312 82-83 3.8819641769232014 2312 84-85 4.356036967456852 2312 86-87 4.465626218268028 2312 88-89 4.618063968903098 2312 90-91 4.404962251894911 2312 92-93 4.701036417177356 2312 94-95 4.766111792517037 2312 96-97 4.652442188105319 2312 98-99 4.649714775537127 2312 100-101 4.132291467269136 2313 1 -0.40662913656742106 2313 2 -0.31029082079780324 2313 3 -0.3804750040770024 2313 4 -0.21819752746505117 2313 5 -0.36818783310919656 2313 6 -0.3543748403083242 2313 7 -0.280163332113581 2313 8 -0.18806750133413885 2313 9 -0.19223702343221305 2313 10-11 -0.2514558441097279 2313 12-13 -0.23489812055345283 2313 14-15 -0.2523281956519341 2313 16-17 -0.08005277362035201 2313 18-19 -0.24049888674696263 2313 20-21 -0.2054845125086402 2313 22-23 -0.171782874088656 2313 24-25 -0.299152558565531 2313 26-27 -0.2394283775674495 2313 28-29 0.014832503161287036 2313 30-31 0.02187392822433054 2313 32-33 0.0948261708810989 2313 34-35 0.2963175621064593 2313 36-37 0.3166461615197633 2313 38-39 0.331619908077883 2313 40-41 0.411009985310713 2313 42-43 0.43909029895610985 2313 44-45 0.5686719202265991 2313 46-47 0.7087046777786767 2313 48-49 0.9476472137631902 2313 50-51 0.9277483623350946 2313 52-53 1.0115954147920903 2313 54-55 1.0498585877499877 2313 56-57 1.3551356772670218 2313 58-59 1.547465944821731 2313 60-61 1.6528101022439614 2313 62-63 1.7823433508193745 2313 64-65 2.006642952160856 2313 66-67 2.204435285168959 2313 68-69 2.523371861290318 2313 70-71 2.7727859021706003 2313 72-73 3.1681042318062467 2313 74-75 3.3290642292060006 2313 76-77 3.331838294267417 2313 78-79 3.5212414827642142 2313 80-81 3.632662162692025 2313 82-83 3.9664387513448496 2313 84-85 4.113988907149398 2313 86-87 4.2761128679334846 2313 88-89 4.427210782970054 2313 90-91 4.436973961652566 2313 92-93 4.625061637963874 2313 94-95 4.592597430181993 2313 96-97 4.569189544496922 2313 98-99 4.456460050376734 2313 100-101 4.074201405203754 2314 1 -0.589133486463421 2314 2 -0.5059539603175303 2314 3 -0.34789931305280675 2314 4 -0.21909123981082246 2314 5 -0.3369282690056181 2314 6 -0.3153782706334738 2314 7 -0.30559711734490236 2314 8 -0.3606329764788043 2314 9 -0.27881748800677286 2314 10-11 -0.4775298220967059 2314 12-13 -0.2776556854051577 2314 14-15 -0.2842343108689178 2314 16-17 -0.23776820682464717 2314 18-19 -0.32784306270379204 2314 20-21 -0.36733101856448513 2314 22-23 -0.30187265976772437 2314 24-25 -0.23300305604060867 2314 26-27 -0.2552366895527598 2314 28-29 -0.20513877973214534 2314 30-31 -0.282672368413067 2314 32-33 -0.09331382705021696 2314 34-35 -0.0023731121132541944 2314 36-37 0.08265027195894703 2314 38-39 0.2552777278306593 2314 40-41 0.42175127064122364 2314 42-43 0.4075224696250572 2314 44-45 0.47643251725336455 2314 46-47 0.5607924507452751 2314 48-49 0.7209892941186595 2314 50-51 0.6060469862377005 2314 52-53 0.8921575609202606 2314 54-55 1.0499184519538858 2314 56-57 1.3095719335793348 2314 58-59 1.5122518010002892 2314 60-61 1.6548083799968651 2314 62-63 1.8033445282667913 2314 64-65 1.9703315931229888 2314 66-67 2.0263797488990427 2314 68-69 2.4072802924372745 2314 70-71 2.7063872016134773 2314 72-73 2.9324928943053266 2314 74-75 3.1976628329885664 2314 76-77 3.470201029544363 2314 78-79 3.662838744738476 2314 80-81 3.961471084654608 2314 82-83 4.061042081919943 2314 84-85 4.365930243063385 2314 86-87 4.409495096270437 2314 88-89 4.481724490810066 2314 90-91 4.541611896571322 2314 92-93 4.70954455293262 2314 94-95 4.65319381934324 2314 96-97 4.6710855926093195 2314 98-99 4.665130370767116 2314 100-101 4.103141497075827 2315 1 -0.476762342402683 2315 2 -0.4692538611820751 2315 3 -0.35743639884661604 2315 4 -0.27767871408664746 2315 5 -0.24993707701597145 2315 6 -0.12362186041151091 2315 7 -0.24184163668572012 2315 8 -0.2608970690267185 2315 9 -0.3504106135407383 2315 10-11 -0.3006574986196 2315 12-13 -0.290304187089653 2315 14-15 -0.3840746598042202 2315 16-17 -0.2006579817683587 2315 18-19 -0.33720098601754955 2315 20-21 -0.34090581209946436 2315 22-23 -0.31710843893477403 2315 24-25 -0.3136072819243694 2315 26-27 -0.2532632156745507 2315 28-29 -0.15561370302978617 2315 30-31 -0.2470645391302284 2315 32-33 -0.14868032262539543 2315 34-35 -0.04336009756264758 2315 36-37 0.17781132341266215 2315 38-39 0.21816422848350214 2315 40-41 0.16268895702393138 2315 42-43 0.46200381523436107 2315 44-45 0.5621450566791779 2315 46-47 0.5603436210272079 2315 48-49 0.7105063580435669 2315 50-51 0.7961186478714737 2315 52-53 1.0478573121413177 2315 54-55 1.273403664248832 2315 56-57 1.6203381545842248 2315 58-59 1.7230741551046052 2315 60-61 1.896733558924268 2315 62-63 2.0589900773804857 2315 64-65 2.0931961677808495 2315 66-67 2.2740319983872475 2315 68-69 2.6102252903954586 2315 70-71 3.0668116538162167 2315 72-73 3.2973405776521822 2315 74-75 3.5189770561051255 2315 76-77 3.470236710919256 2315 78-79 3.6538913671264304 2315 80-81 3.887612905442424 2315 82-83 4.100773166929265 2315 84-85 4.238410718215043 2315 86-87 4.566964139949139 2315 88-89 4.598641022251321 2315 90-91 4.702724050604711 2315 92-93 5.030347063155123 2315 94-95 5.143686537991989 2315 96-97 5.28581859181946 2315 98-99 5.336207871049211 2315 100-101 4.7680614834816435 2316 1 -0.8133191715926387 2316 2 -0.7512533858895196 2316 3 -0.5895795797582437 2316 4 -0.552153204992635 2316 5 -0.5255900862524499 2316 6 -0.5002402186189201 2316 7 -0.5263626725903805 2316 8 -0.5084027544063545 2316 9 -0.48501877562197393 2316 10-11 -0.5154941054710704 2316 12-13 -0.4658982380327288 2316 14-15 -0.602136489279097 2316 16-17 -0.4167219150927224 2316 18-19 -0.6298800236731523 2316 20-21 -0.49642441844325447 2316 22-23 -0.4995590221070856 2316 24-25 -0.6081636118628708 2316 26-27 -0.48498371645418814 2316 28-29 -0.4912722357615138 2316 30-31 -0.42559225891741903 2316 32-33 -0.3506577242126383 2316 34-35 -0.1855009829536769 2316 36-37 -0.11566750405225434 2316 38-39 -0.21590754587297312 2316 40-41 -0.1405225216150967 2316 42-43 -0.00414373137540025 2316 44-45 0.35819857436214164 2316 46-47 0.46837434927383015 2316 48-49 0.57159741347796 2316 50-51 0.7957183887269466 2316 52-53 0.8016745459645378 2316 54-55 1.1821658670938575 2316 56-57 1.299472697992556 2316 58-59 1.2653386068475143 2316 60-61 1.4748978968300719 2316 62-63 1.7130013782057034 2316 64-65 2.255057323428158 2316 66-67 2.311032617433142 2316 68-69 2.4644358547114784 2316 70-71 2.7706262813008173 2316 72-73 3.2986728861494257 2316 74-75 3.2741138095590934 2316 76-77 3.575734579873199 2316 78-79 3.7773719202789025 2316 80-81 4.005613105531527 2316 82-83 4.32232975653033 2316 84-85 4.667390581092491 2316 86-87 5.0595331609759775 2316 88-89 5.114594242844049 2316 90-91 5.3053774490112495 2316 92-93 5.402316830274721 2316 94-95 5.454950307877869 2316 96-97 5.55395984177413 2316 98-99 5.582651918625089 2316 100-101 5.123176091151594 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5157.0 3 1785.0 4 1794.0 5 2146.0 6 2563.0 7 3263.0 8 4265.0 9 5433.0 10 7152.0 11 8824.0 12 11405.0 13 14038.0 14 16749.0 15 20731.0 16 24675.0 17 30595.0 18 31296.0 19 29635.0 20 31506.0 21 34029.0 22 38297.0 23 42769.0 24 47708.0 25 52106.0 26 56739.0 27 61538.0 28 67345.0 29 73356.0 30 80129.0 31 88245.0 32 99355.0 33 114801.0 34 137076.0 35 169915.0 36 207978.0 37 226125.0 38 126071.0 39 8866.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.719300871433035 18.612078302561308 14.551172040520575 23.117448785485085 2 15.563931629873567 19.762430485552027 40.09271447779593 24.580923406778474 3 19.286165970065024 23.781954868275527 29.83616634796827 27.09571281369118 4 12.826021457484574 16.495831742042622 36.91601310383814 33.76213369663466 5 13.67534590637145 37.33284215703666 34.14822571801777 14.843586218574123 6 32.26305350004939 36.08809235365143 17.07174864390346 14.577105502395721 7 28.569953130644237 32.96845802691247 20.201533695504864 18.260055146938427 8 26.79209370350834 34.34950594463497 19.456052927630072 19.402347424226623 9 26.340667385108507 16.764230665173354 20.190432430444403 36.70466951927374 10-11 25.119376050428638 26.465209400555388 26.890771543611176 21.524643005404794 12-13 26.047744903727278 23.83232353359041 27.37701787814087 22.74291368454145 14-15 22.74304331091851 25.255592454594368 25.857457647269815 26.1439065872173 16-17 22.086971810940394 28.414308090153806 26.808046071401403 22.690674027504397 18-19 22.15614021520999 26.600340605992518 29.336145771908846 21.907373406888645 20-21 22.683134696353594 25.782818810274122 29.794766000187934 21.739280493184353 22-23 22.370241290644998 25.767307878318906 29.74273951733811 22.11971131369799 24-25 22.151484780826465 26.01731156739826 29.784124411900017 22.04707923987526 26-27 21.762724862033227 26.372626424825302 29.48818395771286 22.376464755428607 28-29 21.85486966957163 26.80804563303135 29.337873662764157 21.99921103463286 30-31 22.029318849415287 26.302393776483452 29.291818030664118 22.37646934343714 32-33 21.685982741742322 26.27503477699645 29.319766483994016 22.719215997267217 34-35 22.429866047564275 26.50993118229512 29.051284027833407 22.008918742307195 36-37 22.57739466815598 25.948850028755892 29.120235911690312 22.353519391397818 38-39 21.982757969301044 26.469348144648087 29.03056647133288 22.51732741471799 40-41 22.50867025818102 26.24132659357708 29.110684629275628 22.13931851896627 42-43 22.26433874512463 26.270593930284253 28.869659128172863 22.595408196418255 44-45 22.233230972107595 26.53202467448702 29.034480865385127 22.200263488020262 46-47 22.614178741710344 26.7851880583632 28.254418420727852 22.3462147791986 48-49 22.374638419409557 26.80886003100432 28.204935756085952 22.611565793500176 50-51 22.45562489327761 27.056235080902496 28.201482805291516 22.286657220528376 52-53 22.764725792462354 26.72905226871845 28.201153020508773 22.305068918310422 54-55 22.340609589729343 26.978124388500063 28.314087271256525 22.367178750514068 56-57 22.466769857368156 27.0902035489475 28.090293710689608 22.35273288299474 58-59 22.43770549932306 26.722366385146024 28.048768817290952 22.791159298239958 60-61 22.122413721458226 26.976476528786375 28.364261852084578 22.53684789767082 62-63 22.220283035617822 26.86120989629885 28.542056550207178 22.376450517876155 64-65 21.98850769243168 27.006519829468985 28.310410168999784 22.694562309099553 66-67 22.09760031187937 26.967088969735308 28.160964408378636 22.774346310006685 68-69 22.549401818921783 26.59470461661207 28.276648171205913 22.579245393260237 70-71 22.311650158250085 26.716231376083105 27.98152682745728 22.990591638209526 72-73 22.387807838969177 26.816558449735794 27.96638642763234 22.829247283662685 74-75 22.358461840300194 27.222740903812255 27.75259366680124 22.66620358908631 76-77 22.48335221773569 26.797256569392058 27.907704760607142 22.81168645226511 78-79 22.61065369094963 27.0616080443267 27.683790687735332 22.64394757698833 80-81 22.771099023480883 27.007878338824483 27.501804497720013 22.719218139974622 82-83 22.7738793651881 27.083521174080648 27.558642831921524 22.583956628809727 84-85 22.81037541314384 27.07669944282875 27.495963001653077 22.61696214237433 86-87 22.925746266965305 26.97399933214099 27.270657669877192 22.82959673101651 88-89 22.853981336810694 27.055352549998602 27.127643630664615 22.96302248252609 90-91 22.80719429932857 27.11574407227446 27.144382435238136 22.93267919315883 92-93 23.04695164045761 26.623274407138542 27.344225977578844 22.985547974825003 94-95 22.839918055890358 26.494959852877425 27.439261686897026 23.22586040433519 96-97 23.094157075721906 26.12009657859302 27.461577870142794 23.324168475542272 98-99 22.87158441110962 26.287683339928154 27.03508485150577 23.805647397456458 100-101 23.657523886353896 25.239997983210227 27.379988403458793 23.722489726977084 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 643.0 1 441.0 2 337.0 3 493.0 4 431.0 5 353.5 6 705.0 7 1664.5 8 2738.5 9 3171.5 10 2692.0 11 2037.5 12 1902.5 13 2022.5 14 2197.5 15 2327.5 16 2505.5 17 2777.5 18 3027.5 19 3347.5 20 3858.5 21 4657.0 22 5632.5 23 6867.0 24 8390.5 25 10426.5 26 13427.0 27 16943.0 28 19407.5 29 21542.0 30 24052.0 31 27724.5 32 33086.5 33 35776.5 34 36714.0 35 39330.0 36 43455.5 37 47305.0 38 49829.0 39 52044.5 40 54851.5 41 58077.5 42 59915.5 43 61141.0 44 63143.5 45 66196.5 46 68123.5 47 69300.5 48 70429.0 49 69525.0 50 68205.0 51 67302.5 52 66406.5 53 66368.0 54 67388.5 55 65307.5 56 60679.5 57 56359.5 58 52775.0 59 47853.5 60 39904.0 61 33225.5 62 28012.0 63 21967.5 64 16472.5 65 12790.0 66 9857.5 67 7549.5 68 5807.5 69 4220.0 70 3032.0 71 2156.5 72 1523.5 73 1094.0 74 759.5 75 562.0 76 430.5 77 303.0 78 193.0 79 103.5 80 56.5 81 35.5 82 20.0 83 15.0 84 13.5 85 9.0 86 5.5 87 5.5 88 4.5 89 2.0 90 3.5 91 3.5 92 2.5 93 2.0 94 1.0 95 0.5 96 1.0 97 0.5 98 0.0 99 1.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3927553312582475 2 0.02221147744099604 3 0.041552083648121844 4 0.03515558107441097 5 0.05580570749347759 6 0.05479838425352311 7 0.029262740120677325 8 0.028255416880722858 9 0.030672992656613583 10-11 0.028003586070734242 12-13 0.029111641634684157 14-15 0.02336989916694368 16-17 0.027248093640768384 18-19 0.023596546895933438 20-21 0.036540650529348365 22-23 0.023571363814934576 24-25 0.04487625033997159 26-27 0.03742205836430852 28-29 0.02931310628267505 30-31 0.03676729825833812 32-33 0.03379569470047244 34-35 0.03278837146051797 36-37 0.03271282221752138 38-39 0.015663876381291995 40-41 0.011332386449487775 42-43 0.011433118773483223 44-45 0.008587430620611848 46-47 0.015361679409305653 48-49 0.01271745590442517 50-51 0.008511881377615263 52-53 0.0033241666918497476 54-55 0.003802645230828121 56-57 0.0070008965176835605 58-59 0.0026190404238816195 60-61 0.005162531604766653 62-63 0.004608503822791696 64-65 0.006648333383699495 66-67 0.0038781944738247058 68-69 0.005439545495754133 70-71 0.015663876381291995 72-73 0.012591540499430861 74-75 0.009645120022564041 76-77 0.006824614950691527 78-79 0.005565460900748441 80-81 0.007202361165674454 82-83 0.009015542997592498 84-85 0.0037270959878315355 86-87 5.54027781974958E-4 88-89 0.02163226657802222 90-91 0.018157001400179305 92-93 0.011911597312461596 94-95 0.022589223655978964 96-97 0.017174861241223697 98-99 0.01589052411028175 100-101 0.10627260181519647 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1985460.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.26138875364983 #Duplication Level Percentage of deduplicated Percentage of total 1 76.3736588677814 42.20514453231103 2 13.300097772348558 14.699637469186113 3 4.535137069376556 7.518539179257179 4 1.9778057529418507 4.3718517037009885 5 1.0797642158262075 2.9834635046525957 6 0.6070880099859205 2.0129115916506963 7 0.4110808944388732 1.5901830781749262 8 0.28529059517283006 1.2612443590084719 9 0.20239046668488286 1.0065940433555334 >10 1.105768288508952 11.166415182719309 >50 0.07534310021742525 2.847317684344555 >100 0.04053749730267827 4.030429927035568 >500 0.0035675955626705744 1.3670097474885192 >1k 0.002286920232481135 2.1104179771522324 >5k 1.8295361859849079E-4 0.8288400199623456 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9089 0.4577780463973084 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7307 0.36802554571736523 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4655 0.23445448409940267 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2561 0.12898774087616974 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2393 0.12052622566055221 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2308 0.11624510189074573 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2247 0.1131727660088846 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2152 0.10838798061910085 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.036616199772345E-5 2 0.0 0.0 0.0 0.0 5.036616199772345E-5 3 0.0 0.0 0.0 0.0 5.036616199772345E-5 4 0.0 0.0 0.0 0.0 5.036616199772345E-5 5 0.0 0.0 0.0 0.0 5.036616199772345E-5 6 0.0 0.0 0.0 0.0 5.036616199772345E-5 7 0.0 0.0 0.0 0.0 5.036616199772345E-5 8 0.0 0.0 0.0 0.0 5.036616199772345E-5 9 0.0 0.0 0.0 5.036616199772345E-5 5.036616199772345E-5 10-11 0.0 0.0 0.0 5.036616199772345E-5 5.036616199772345E-5 12-13 0.0 0.0 0.0 5.036616199772345E-5 1.007323239954469E-4 14-15 0.0 0.0 0.0 5.036616199772345E-5 1.2591540499430864E-4 16-17 0.0 0.0 0.0 1.007323239954469E-4 1.5109848599317036E-4 18-19 0.0 0.0 0.0 1.7628156699203208E-4 1.5109848599317036E-4 20-21 0.0 0.0 0.0 3.7774621498292587E-4 2.518308099886173E-4 22-23 0.0 0.0 0.0 5.036616199772346E-4 3.7774621498292587E-4 24-25 0.0 0.0 0.0 7.806755109647135E-4 4.029292959817876E-4 26-27 0.0 0.0 0.0 0.0012087878879453629 4.029292959817876E-4 28-29 0.0 0.0 0.0 0.003701912906832674 4.029292959817876E-4 30-31 0.0 0.0 0.0 0.011005006396502573 4.281123769806493E-4 32-33 0.0 0.0 0.0 0.029287923201676187 5.54027781974958E-4 34-35 0.0 0.0 0.0 0.055780524412478716 5.54027781974958E-4 36-37 0.0 0.0 0.0 0.09871767751553796 5.54027781974958E-4 38-39 0.0 0.0 0.0 0.16567948989151127 5.54027781974958E-4 40-41 0.0 0.0 0.0 0.2375268199812638 5.54027781974958E-4 42-43 0.0 0.0 0.0 0.31335307686883646 5.792108629738197E-4 44-45 0.0 0.0 0.0 0.4020730712278263 6.547601059704049E-4 46-47 0.0 0.0 0.0 0.5028557613852709 7.051262679681283E-4 48-49 0.0 0.0 0.0 0.6106897142223968 7.554924299658517E-4 50-51 0.0 2.5183080998861724E-5 0.0 0.7237365648262871 8.058585919635752E-4 52-53 0.0 7.554924299658518E-5 0.0 0.8345924873832764 8.814078349601603E-4 54-55 0.0 1.007323239954469E-4 0.0 0.9512908847320016 9.569570779567456E-4 56-57 5.036616199772345E-5 1.007323239954469E-4 0.0 1.0831243137610427 9.569570779567456E-4 58-59 5.036616199772345E-5 1.007323239954469E-4 0.0 1.2229659625477218 9.569570779567456E-4 60-61 5.036616199772345E-5 1.007323239954469E-4 0.0 1.3765827566407784 9.569570779567456E-4 62-63 5.036616199772345E-5 1.007323239954469E-4 0.0 1.5260443423690226 0.001007323239954469 64-65 5.036616199772345E-5 1.007323239954469E-4 0.0 1.6809202905120224 0.001082872482951054 66-67 7.554924299658518E-5 1.007323239954469E-4 0.0 1.8559427034541114 0.0011332386449487777 68-69 1.007323239954469E-4 1.007323239954469E-4 0.0 2.051791524382259 0.0011584217259476394 70-71 1.007323239954469E-4 1.007323239954469E-4 0.0 2.253785017074129 0.0011836048069465011 72-73 1.007323239954469E-4 1.007323239954469E-4 0.0 2.457868705488904 0.0013347032929396715 74-75 1.007323239954469E-4 1.007323239954469E-4 0.0 2.6570668761899006 0.00146061869793398 76-77 1.007323239954469E-4 1.007323239954469E-4 0.0 2.8717778247861956 0.0015109848599317035 78-79 1.007323239954469E-4 1.007323239954469E-4 0.0 3.114290894805234 0.0015109848599317035 80-81 1.007323239954469E-4 1.007323239954469E-4 0.0 3.359045259033171 0.0015109848599317035 82-83 1.007323239954469E-4 1.2591540499430864E-4 0.0 3.620017527424375 0.001561351021929427 84-85 1.007323239954469E-4 1.7628156699203208E-4 0.0 3.878798867768678 0.001561351021929427 86-87 1.007323239954469E-4 2.014646479908938E-4 0.0 4.14425372457768 0.0016117171839271503 88-89 1.007323239954469E-4 2.014646479908938E-4 0.0 4.422073474157122 0.0016620833459248738 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4005 0.0 55.75247 1 GTATCAA 7450 0.0 42.58789 1 TATCAAC 9530 0.0 33.11529 2 ATCAACG 9560 0.0 32.961655 3 TCAACGC 9615 0.0 32.773113 4 CAACGCA 9885 0.0 31.974104 5 AACGCAG 10230 0.0 31.076937 6 ACGCAGA 11595 0.0 27.290005 7 CGCAGAG 11555 0.0 27.178886 8 TGGTATC 1795 0.0 26.213448 2 GCAGAGT 12575 0.0 24.744503 9 GTGGTAT 1960 0.0 24.585897 1 GAGTACT 7795 0.0 23.950298 12-13 TACATGG 7010 0.0 22.7811 2 GTACATG 7245 0.0 22.71964 1 GTACTTT 8280 0.0 22.26083 14-15 AGAGTAC 11260 0.0 21.98031 10-11 AGTACTT 8115 0.0 21.805813 12-13 CAGAGTA 11930 0.0 21.76127 10-11 ACATGGG 7310 0.0 21.195988 3 >>END_MODULE