FastQCFastQC Report
Thu 26 May 2016
SRR938151_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938151_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1985460
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88370.4450857735738821No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74230.3738680205091012No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47780.24064952202512263No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA25360.12772858682622668No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA24560.1236992938664088No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC23240.1170509604827093No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA22660.11412972308684134No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA22520.11342459681887322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43150.055.774891
GTATCAA77600.041.9240651
TATCAAC98300.032.85462
ATCAACG98500.032.728873
TCAACGC98500.032.728054
CAACGCA101250.031.7913785
AACGCAG104400.030.8905286
TGGTATC18400.027.1425972
ACGCAGA118300.027.0602157
CGCAGAG118000.027.0485088
GTGTAGC13100.025.7784041
GTGGTAT20650.025.5665361
GCAGAGT132250.023.8820239
GAGTACT78650.022.52450612-13
TAAGGTG12050.021.7015765
GTACTTT82000.021.57533514-15
CAGAGTA125600.021.49740210-11
GTAAGGT13050.020.7678224
GTACATG76050.020.763851
AGAGTAC119350.020.65332810-11
Produced by FastQC (version 0.11.5)