FastQCFastQC Report
Thu 26 May 2016
SRR938150_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938150_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1976928
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93080.47083151232619497No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74380.3762403081953415No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46350.23445466906230275No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA25070.12681291377328865No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA24070.12175456061121093No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA23400.11836546399261885No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA22190.1122448566665048No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC20920.10582074815066607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42100.050.8889471
GTATCAA75500.042.343531
ATCAACG97000.032.488233
TATCAAC97350.032.4666182
TCAACGC97200.032.4213874
CAACGCA99750.031.4496175
AACGCAG103350.030.4391736
ACGCAGA117650.026.4909977
CGCAGAG118250.026.236058
TACATGG71150.024.1147982
GCAGAGT128500.023.99549
GTACATG74050.023.5194421
TGGTATC20150.022.8795782
ACATGGG72250.022.7626363
GAGTACT78600.022.6624712-13
TAAGGTG11300.022.2936785
AGAGTAC113750.021.33870110-11
GTACTTT83750.021.24268714-15
AGTACTT80750.021.14729712-13
CAGAGTA121100.020.98495910-11
Produced by FastQC (version 0.11.5)