##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938150_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1976928 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.844190582560415 33.0 31.0 34.0 30.0 34.0 2 32.04187911749948 34.0 31.0 34.0 30.0 34.0 3 32.11942771815666 34.0 31.0 34.0 30.0 34.0 4 35.64275481959889 37.0 35.0 37.0 33.0 37.0 5 35.65313203111089 37.0 35.0 37.0 33.0 37.0 6 35.6751004588938 37.0 35.0 37.0 33.0 37.0 7 35.61874686382104 37.0 35.0 37.0 33.0 37.0 8 35.58649025154179 37.0 35.0 37.0 33.0 37.0 9 37.2229165654996 39.0 37.0 39.0 34.0 39.0 10-11 37.13812187393775 39.0 37.0 39.0 34.0 39.0 12-13 37.035209678855274 39.0 37.0 39.0 33.0 39.0 14-15 38.21845029257514 40.0 38.0 41.0 33.0 41.0 16-17 38.06585950525259 40.0 38.0 41.0 33.0 41.0 18-19 38.21555236204859 40.0 38.0 41.0 33.5 41.0 20-21 38.19519072014762 40.0 38.0 41.0 33.5 41.0 22-23 38.102882350798815 40.0 38.0 41.0 33.0 41.0 24-25 37.99563792915068 40.0 38.0 41.0 33.0 41.0 26-27 37.81491005236407 40.0 38.0 41.0 33.0 41.0 28-29 37.60606127284352 40.0 37.5 41.0 32.0 41.0 30-31 37.38471304974182 40.0 37.0 41.0 32.0 41.0 32-33 37.128404777513396 40.0 36.5 41.0 31.0 41.0 34-35 36.888360881124655 39.5 36.0 41.0 30.5 41.0 36-37 36.620847344971594 39.0 36.0 41.0 30.0 41.0 38-39 36.319214710905 39.0 35.0 40.5 30.0 41.0 40-41 36.096303962511534 39.0 35.0 40.0 29.5 41.0 42-43 35.91013911482867 39.0 35.0 40.0 28.5 41.0 44-45 35.63791726355234 39.0 35.0 40.0 27.5 41.0 46-47 35.24547833810842 38.5 35.0 40.0 26.0 41.0 48-49 34.84048103926901 38.0 34.0 40.0 24.0 41.0 50-51 34.135106336700176 37.5 33.5 39.5 23.5 40.5 52-53 34.2482897707959 38.0 34.0 40.0 24.0 40.5 54-55 34.34577789378268 38.0 34.0 40.0 23.0 41.0 56-57 34.034230381683095 38.0 34.0 40.0 22.5 41.0 58-59 33.703210992003754 37.5 33.0 40.0 21.5 41.0 60-61 33.27784876333382 37.0 33.0 40.0 19.0 41.0 62-63 32.7328567352984 36.5 32.0 39.5 16.0 41.0 64-65 32.207229347755714 36.0 31.5 39.0 10.5 40.5 66-67 31.664248773855192 35.0 31.0 39.0 7.5 40.0 68-69 31.054385389857394 35.0 30.5 38.0 4.5 40.0 70-71 30.490063623966073 35.0 29.5 37.5 2.0 40.0 72-73 29.841716036193528 34.0 29.0 37.0 2.0 39.0 74-75 29.153816173376065 34.0 28.0 36.0 2.0 39.0 76-77 28.48253451820198 34.0 26.5 36.0 2.0 38.5 78-79 27.75023496050438 33.5 26.0 35.0 2.0 37.0 80-81 27.083234189611357 33.0 25.0 35.0 2.0 37.0 82-83 26.33522819242785 32.0 23.5 35.0 2.0 36.0 84-85 25.66917004564658 32.0 20.5 35.0 2.0 36.0 86-87 24.96804284222794 31.5 18.5 34.0 2.0 35.0 88-89 24.123715178296834 31.0 11.0 34.0 2.0 35.0 90-91 23.28057218067628 30.5 4.5 34.0 2.0 35.0 92-93 22.36332228589003 29.5 2.0 34.0 2.0 35.0 94-95 21.39428370684213 29.0 2.0 34.0 2.0 35.0 96-97 20.174356628061318 28.0 2.0 34.0 2.0 35.0 98-99 18.655926518315287 25.5 2.0 33.5 2.0 35.0 100-101 14.992377820537722 10.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3950270759978487 1101 2 0.34358557249434796 1101 3 0.35765508675665103 1101 4 0.14640814511156464 1101 5 0.25424201215644615 1101 6 0.2364407332533105 1101 7 0.2709462355139678 1101 8 0.22984525388132937 1101 9 0.2658204274625646 1101 10-11 0.35914798147758376 1101 12-13 0.41329775859171036 1101 14-15 0.5847713628073024 1101 16-17 0.6856829350052323 1101 18-19 0.6315139656096633 1101 20-21 0.5683047019522505 1101 22-23 0.6279046695294923 1101 24-25 0.5988521681424217 1101 26-27 0.6197693378261349 1101 28-29 0.734891326387789 1101 30-31 0.7917171480321414 1101 32-33 0.7286178816896722 1101 34-35 0.843984505175861 1101 36-37 0.8701678962192432 1101 38-39 0.9400581468537794 1101 40-41 1.2056798216186237 1101 42-43 1.2597320523574425 1101 44-45 1.210019963207344 1101 46-47 1.3835224711345688 1101 48-49 1.6702062349786004 1101 50-51 1.3946873932366586 1101 52-53 1.5770685714062083 1101 54-55 1.6080646137434584 1101 56-57 1.776002281324736 1101 58-59 1.6889924346207152 1101 60-61 1.6203672893801055 1101 62-63 1.6143826127593854 1101 64-65 1.7386177371034464 1101 66-67 1.8636470701066834 1101 68-69 2.059973736601915 1101 70-71 2.4347425392504825 1101 72-73 2.6016663864475085 1101 74-75 2.78457143180432 1101 76-77 2.808974211287744 1101 78-79 3.097532909490429 1101 80-81 3.1766810264541085 1101 82-83 3.3294369684845684 1101 84-85 3.375129231178054 1101 86-87 3.617980688802252 1101 88-89 3.943615521790008 1101 90-91 4.264399680841603 1101 92-93 4.475952343210221 1101 94-95 4.786893699596014 1101 96-97 4.977381077733707 1101 98-99 5.135619728458348 1101 100-101 5.109567597614367 1102 1 0.2835045856628362 1102 2 0.2244810147249794 1102 3 0.17906038454219697 1102 4 0.05938561413096721 1102 5 0.2104264045182802 1102 6 0.3438050469626006 1102 7 0.12327882567720394 1102 8 0.19820133319485933 1102 9 0.3890115323428347 1102 10-11 0.23622357160484597 1102 12-13 0.3279107126728604 1102 14-15 0.49455965261432056 1102 16-17 0.566354993097768 1102 18-19 0.4267350109608046 1102 20-21 0.41868197294102316 1102 22-23 0.45752937085107703 1102 24-25 0.3968181231337624 1102 26-27 0.4749546150623374 1102 28-29 0.40373875085696653 1102 30-31 0.6017523431109595 1102 32-33 0.5506605155769009 1102 34-35 0.4000890722737296 1102 36-37 0.5672035337352526 1102 38-39 0.6917509355273879 1102 40-41 0.7366345997403769 1102 42-43 0.6164966771054026 1102 44-45 0.6480427753754299 1102 46-47 0.6927031094434994 1102 48-49 0.9624315008073907 1102 50-51 0.9376249562872943 1102 52-53 0.6582093197380701 1102 54-55 0.7104769958546058 1102 56-57 0.6713542254497185 1102 58-59 0.6186262645409215 1102 60-61 0.5090290368204293 1102 62-63 0.5177828824861521 1102 64-65 0.7146668263091769 1102 66-67 0.8517683978446975 1102 68-69 0.9914984191332472 1102 70-71 1.0715007739794054 1102 72-73 1.4389319580377098 1102 74-75 1.3927643157618412 1102 76-77 1.6217929626287173 1102 78-79 1.9065669571643689 1102 80-81 2.050107342671428 1102 82-83 2.1325272656504417 1102 84-85 2.209736514866915 1102 86-87 2.4190995085077667 1102 88-89 2.8266843367646395 1102 90-91 3.1295974316082074 1102 92-93 3.5668851793791916 1102 94-95 3.7198009983437323 1102 96-97 4.079089658216631 1102 98-99 4.025175012451054 1102 100-101 4.176094758860069 1103 1 0.6082302061666489 1103 2 0.6009432539784427 1103 3 0.5299182277265615 1103 4 0.42963310830008083 1103 5 0.44637758613099976 1103 6 0.5339729404192752 1103 7 0.5365428431642982 1103 8 0.45155695339497015 1103 9 0.5887833328697383 1103 10-11 0.634276917844879 1103 12-13 0.7103653024770011 1103 14-15 0.9324212359480697 1103 16-17 0.9133442862522045 1103 18-19 0.8755016151058399 1103 20-21 0.8483978038565922 1103 22-23 0.9444607606958115 1103 24-25 0.9418734872630026 1103 26-27 1.0642491919034 1103 28-29 1.03668512667182 1103 30-31 1.0104250763878326 1103 32-33 1.0883291903938073 1103 34-35 0.968935461659072 1103 36-37 1.1440536275176996 1103 38-39 1.1193304718472774 1103 40-41 1.108804643715672 1103 42-43 1.0901218771547647 1103 44-45 1.0563848962081295 1103 46-47 1.1482655292651813 1103 48-49 1.258817099483096 1103 50-51 1.3357972845639239 1103 52-53 1.3005810334599843 1103 54-55 1.269269114822805 1103 56-57 1.4215876724342849 1103 58-59 1.4069779808231644 1103 60-61 1.387697921922232 1103 62-63 1.4945250659045541 1103 64-65 1.6397506231086183 1103 66-67 1.7938973805233012 1103 68-69 1.9945137199540532 1103 70-71 2.1532856429242493 1103 72-73 2.338065176784358 1103 74-75 2.448395625990493 1103 76-77 2.5890934697343866 1103 78-79 2.73481667566325 1103 80-81 2.748537663984436 1103 82-83 2.832423622493522 1103 84-85 2.877466587490165 1103 86-87 2.930912560241307 1103 88-89 3.3038705403842314 1103 90-91 3.4628945641603437 1103 92-93 3.8533635532887978 1103 94-95 4.308713448708616 1103 96-97 4.79231507862303 1103 98-99 5.18162908634601 1103 100-101 4.990078742609315 1104 1 0.32521662316175437 1104 2 0.3645450690586003 1104 3 0.35354949613489595 1104 4 0.24652622678235048 1104 5 0.298693359188519 1104 6 0.3018764735874484 1104 7 0.2863229350127128 1104 8 0.34472947219489214 1104 9 0.34224748476299993 1104 10-11 0.4158479307356657 1104 12-13 0.38390241736413344 1104 14-15 0.5386886866688059 1104 16-17 0.6814273711771079 1104 18-19 0.5131892552417128 1104 20-21 0.5639531078496915 1104 22-23 0.5009822857024062 1104 24-25 0.5642012441177826 1104 26-27 0.6294174165093054 1104 28-29 0.7386483626801237 1104 30-31 0.6088909177776216 1104 32-33 0.5890260222116055 1104 34-35 0.6412177662691363 1104 36-37 0.7700427011947966 1104 38-39 0.9144720065120495 1104 40-41 0.8413267337118029 1104 42-43 0.7625495331869487 1104 44-45 0.7632557092321619 1104 46-47 0.587049153324152 1104 48-49 0.7549594727400759 1104 50-51 0.7754533591589521 1104 52-53 0.7535336851487315 1104 54-55 1.0132608000374503 1104 56-57 1.114453277431366 1104 58-59 0.988772869104352 1104 60-61 0.9537731394021591 1104 62-63 0.8574607268670604 1104 64-65 0.9798926160578745 1104 66-67 1.1821688203710643 1104 68-69 1.3442506167798491 1104 70-71 1.4762910452090985 1104 72-73 1.64373357444396 1104 74-75 1.6177982624258895 1104 76-77 1.672073570611694 1104 78-79 1.9117510807894682 1104 80-81 2.066786825734237 1104 82-83 2.191954811146669 1104 84-85 2.285229482042322 1104 86-87 2.3570492425626064 1104 88-89 2.84370965517013 1104 90-91 3.1408081248666235 1104 92-93 3.362352085232594 1104 94-95 3.5917311357536654 1104 96-97 3.8820882209634533 1104 98-99 4.095296497667963 1104 100-101 4.475573354688601 1105 1 0.33573499888987257 1105 2 0.2129379785838026 1105 3 0.15660757768373657 1105 4 0.044015016959612296 1105 5 0.2090388731641326 1105 6 0.22838822818661697 1105 7 0.1735290700334602 1105 8 0.2103184593297911 1105 9 0.2570255931316012 1105 10-11 0.26966608017203697 1105 12-13 0.28729207918368616 1105 14-15 0.3167551493935292 1105 16-17 0.5299004256966597 1105 18-19 0.40417739587784496 1105 20-21 0.3552785908149545 1105 22-23 0.3302671618705375 1105 24-25 0.37895420895875276 1105 26-27 0.41835478817255733 1105 28-29 0.49824323759107614 1105 30-31 0.5873020563603646 1105 32-33 0.6274820000853012 1105 34-35 0.6066176531232657 1105 36-37 0.7143052624821422 1105 38-39 0.5887119386245274 1105 40-41 0.7556674494642195 1105 42-43 0.6856328630281254 1105 44-45 0.8170738586685289 1105 46-47 0.806323762551834 1105 48-49 0.839449059129052 1105 50-51 0.9577800783476533 1105 52-53 0.8294446142020817 1105 54-55 1.0132126082761417 1105 56-57 1.1734724703241284 1105 58-59 1.1824298904344488 1105 60-61 1.2479613177535782 1105 62-63 1.4177643349632802 1105 64-65 1.3949546367593797 1105 66-67 1.6421937543693055 1105 68-69 1.6807689715550218 1105 70-71 1.597987814265803 1105 72-73 1.8344325645122659 1105 74-75 2.040492391412183 1105 76-77 1.9810131423336053 1105 78-79 2.1969048753668474 1105 80-81 2.2862217249505967 1105 82-83 2.4328811406106965 1105 84-85 2.570725333360265 1105 86-87 2.710976871108894 1105 88-89 2.938351464665999 1105 90-91 3.1508262491594614 1105 92-93 3.4008227301640765 1105 94-95 3.6042730416330606 1105 96-97 3.711565340353303 1105 98-99 4.29028403066884 1105 100-101 4.295270689474668 1106 1 0.18064561822444958 1106 2 0.15871202021038044 1106 3 0.11854483397583238 1106 4 0.18102696814207064 1106 5 0.24933739952256673 1106 6 0.020880758697579438 1106 7 0.17622821420725643 1106 8 0.24701010869424778 1106 9 0.21109203360357043 1106 10-11 0.11724989037104905 1106 12-13 0.3358102085197885 1106 14-15 0.3744284668594702 1106 16-17 0.25884596172745233 1106 18-19 0.25951111745580846 1106 20-21 0.3267058962284324 1106 22-23 0.11689171243518359 1106 24-25 0.13489435176524722 1106 26-27 0.14865875839588227 1106 28-29 0.19313549970683397 1106 30-31 0.26558863153634604 1106 32-33 0.3081550137076192 1106 34-35 0.28635065054059794 1106 36-37 0.41382101170987085 1106 38-39 0.17006290332802365 1106 40-41 0.6336849964333808 1106 42-43 0.610990512291302 1106 44-45 0.7765063041032789 1106 46-47 1.0930723825953805 1106 48-49 0.9482048194977111 1106 50-51 1.0044916041490453 1106 52-53 1.0421243883449804 1106 54-55 0.5564647478416518 1106 56-57 1.0219140057472345 1106 58-59 1.2198539295289947 1106 60-61 1.3600093503661341 1106 62-63 1.2234272204648704 1106 64-65 1.2619622834905329 1106 66-67 1.3707120103069208 1106 68-69 1.2297175460220267 1106 70-71 1.44321493737311 1106 72-73 1.716896989488209 1106 74-75 1.88929094309092 1106 76-77 1.9762276052900027 1106 78-79 2.1572596127953183 1106 80-81 2.3634448474502143 1106 82-83 2.4179244383268035 1106 84-85 2.654762782086653 1106 86-87 2.4824535511109183 1106 88-89 2.6093160415637584 1106 90-91 3.035724588147662 1106 92-93 3.3903489873170614 1106 94-95 3.5162349852194303 1106 96-97 3.644802667542244 1106 98-99 3.7058946297471813 1106 100-101 3.84605118777821 1107 1 0.20724296807177822 1107 2 0.11214008017371668 1107 3 0.007695130860625454 1107 4 0.0059104667821898715 1107 5 0.006712322947500127 1107 6 0.01205287560178192 1107 7 0.046802413953159316 1107 8 0.10322657358413778 1107 9 0.08693698131800431 1107 10-11 0.035996776715776946 1107 12-13 0.04849818987003118 1107 14-15 0.07160478377177526 1107 16-17 0.09256902339510731 1107 18-19 0.11533964175602307 1107 20-21 0.25338333703620464 1107 22-23 0.28081657471925325 1107 24-25 0.1734198914853593 1107 26-27 0.19716122429166916 1107 28-29 0.321541332044994 1107 30-31 0.33415114181587313 1107 32-33 0.336667633993045 1107 34-35 0.30230591401840456 1107 36-37 0.3799978363416585 1107 38-39 0.37534199410493585 1107 40-41 0.27466189162148424 1107 42-43 0.3923368270344767 1107 44-45 0.386886783838591 1107 46-47 0.351829947734835 1107 48-49 0.37517020637757525 1107 50-51 0.10231413116186161 1107 52-53 0.12001809143158226 1107 54-55 0.301979296507632 1107 56-57 0.35487918286505504 1107 58-59 0.28476550767845765 1107 60-61 0.31214582440926364 1107 62-63 0.3434573499214224 1107 64-65 0.5073857763621916 1107 66-67 0.5941904608328663 1107 68-69 0.8456587459085689 1107 70-71 1.0645250920011016 1107 72-73 1.224592634303871 1107 74-75 1.2715521063644495 1107 76-77 1.6601815243838338 1107 78-79 1.71285549192471 1107 80-81 1.873958692936636 1107 82-83 1.914412831096687 1107 84-85 1.999792017670874 1107 86-87 1.9220729521926252 1107 88-89 2.219979461686137 1107 90-91 2.505367849661333 1107 92-93 2.5973797103048355 1107 94-95 3.039497952923224 1107 96-97 3.2801377981548683 1107 98-99 3.3165791452564655 1107 100-101 3.488553982619015 1108 1 0.23317415303908362 1108 2 0.10917747015309232 1108 3 0.14613149904185008 1108 4 0.14143475986851684 1108 5 0.05782027863378403 1108 6 0.1127935732274068 1108 7 0.05332029135993821 1108 8 0.12648730357016547 1108 9 0.1796782709241853 1108 10-11 0.16956746627837305 1108 12-13 0.10217846351665827 1108 14-15 0.147360923878054 1108 16-17 0.29336571552325097 1108 18-19 0.28974525853253397 1108 20-21 0.22467521277985014 1108 22-23 0.1664787108395842 1108 24-25 0.1180513226750648 1108 26-27 0.19178715078411557 1108 28-29 0.26011297139347533 1108 30-31 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-1.0702497351347944 2316 60-61 -1.2060971238048595 2316 62-63 -1.2631978846353036 2316 64-65 -1.4371123222556292 2316 66-67 -1.6014308028916169 2316 68-69 -1.6695282961123752 2316 70-71 -1.8884432871003654 2316 72-73 -1.7247426758318092 2316 74-75 -2.021086289647016 2316 76-77 -2.211047070181152 2316 78-79 -2.779520322811596 2316 80-81 -2.8304716466129065 2316 82-83 -2.6499953984346725 2316 84-85 -2.494249788920051 2316 86-87 -2.176575835924332 2316 88-89 -2.5220950693194943 2316 90-91 -2.6264106373306717 2316 92-93 -2.8064508404211814 2316 94-95 -3.0364104631738194 2316 96-97 -3.2583431602370148 2316 98-99 -3.5758169138411944 2316 100-101 -3.1490685751568037 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5398.0 3 1683.0 4 1793.0 5 2005.0 6 2258.0 7 2837.0 8 3458.0 9 4395.0 10 5799.0 11 7118.0 12 8997.0 13 11169.0 14 13069.0 15 15525.0 16 18759.0 17 23586.0 18 22948.0 19 19577.0 20 19584.0 21 20688.0 22 22831.0 23 25132.0 24 28693.0 25 31834.0 26 36174.0 27 41727.0 28 47871.0 29 56344.0 30 66255.0 31 79344.0 32 97332.0 33 122074.0 34 159163.0 35 213750.0 36 275622.0 37 300508.0 38 152960.0 39 8668.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.68354935984804 18.63489130578398 14.602802907185334 23.07875642718265 2 15.54053130843368 19.68432212503131 40.20378884297834 24.571357723556662 3 19.256015179727836 23.814944176844435 29.80271718747865 27.12632345594908 4 12.82254260244935 16.525721412273473 36.97444156727613 33.67729441800104 5 13.711772794562883 37.271537958367 34.118331439819684 14.898357807250436 6 32.290580289790505 36.0870687822116 17.05556849363615 14.566782434361741 7 28.598330721923293 33.03398274445025 20.178962561692032 18.18872397193443 8 26.85473635629399 34.299389294743726 19.46473718622158 19.381137162740707 9 26.40575846279044 16.715048604616324 20.178658909361804 36.70053402323143 10-11 25.129190743765683 26.438698134860434 26.920843228825962 21.51126789254792 12-13 26.07960954853037 23.818541687647933 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22.349718037074467 38-39 21.968928984258053 26.442746415286617 29.048416937642674 22.539907662812663 40-41 22.486666941579433 26.249497504154682 29.190600310820088 22.073235243445797 42-43 22.157781279654603 26.317110322528254 28.986382061732936 22.53872633608421 44-45 22.166962079782536 26.51197593051337 29.142640899661316 22.178421090042775 46-47 22.58711761564346 26.783456804928036 28.330058382419647 22.299367197008856 48-49 22.2955499571688 26.849647559684126 28.30274600364407 22.55205647950301 50-51 22.38728608830932 27.09431719008892 28.28402666228198 22.234370059319783 52-53 22.718294873825055 26.726225740306113 28.243340394347616 22.31213899152122 54-55 22.305678504040248 26.983600975537936 28.347593446259022 22.36312707416279 56-57 22.31984846599136 27.113695027689154 28.171630275636883 22.3948262306826 58-59 22.365349264357135 26.779990635455903 28.096187173508913 22.758472926678046 60-61 22.03851216099612 26.959651047573175 28.42728812834722 22.574548663083476 62-63 22.191115903963183 26.893465371179996 28.535438815372917 22.3799799094839 64-65 21.928617197573235 27.027981465596074 28.40490746331785 22.63849387351284 66-67 21.934918742650364 27.000270904205387 28.269600020437963 22.79521033270628 68-69 22.43688946607165 26.605758932659327 28.41401947030609 22.543332130962927 70-71 22.213528464761698 26.767657603234785 28.09772660350496 22.921087328498558 72-73 22.26880701500534 26.889327585308436 28.034876702639888 22.806988697046336 74-75 22.165731059003992 27.275433924310892 27.900721031745228 22.658113984939888 76-77 22.31834509581526 26.912302330987416 27.968155929001675 22.801196644195652 78-79 22.439553623645928 27.182049429634713 27.766428483255325 22.61196846346404 80-81 22.5913055664541 27.10163371933156 27.62984847185532 22.677212242359023 82-83 22.604764252448565 27.207376848265714 27.704741916299902 22.483116982985823 84-85 22.575210735761488 27.302334358301888 27.571375957050474 22.551078948886143 86-87 22.73330392616102 27.153814012450162 27.400950607079373 22.71193145430945 88-89 22.61281817811523 27.27919018797906 27.2353458629097 22.872645770996005 90-91 22.511433998057413 27.293131100145807 27.355168303745486 22.840266598051294 92-93 22.811780392708425 26.83193059845777 27.44466320323411 22.91162580559969 94-95 22.574816999304733 26.683161102345785 27.59558389695085 23.14643800139863 96-97 22.919026351987423 26.257899385252692 27.64020167577399 23.182872586985898 98-99 22.716304678906415 26.512293551615514 27.169118428335935 23.602283341142137 100-101 23.51582593823631 25.50765143674362 27.387037011707026 23.589485613313048 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 612.0 1 430.5 2 372.5 3 509.0 4 409.5 5 385.5 6 778.5 7 1704.0 8 2833.0 9 3299.0 10 2670.5 11 2003.0 12 1945.5 13 2025.5 14 2167.5 15 2335.5 16 2480.0 17 2678.5 18 2953.0 19 3329.5 20 3774.0 21 4427.5 22 5427.0 23 6711.0 24 8117.5 25 10065.0 26 13065.0 27 16584.5 28 19133.5 29 21212.0 30 23682.5 31 27434.0 32 33375.5 33 36196.5 34 36712.0 35 39257.5 36 43310.5 37 47548.0 38 50265.5 39 52241.5 40 55348.5 41 59030.0 42 60234.0 43 61223.5 44 63693.5 45 67211.0 46 69251.5 47 70349.0 48 71289.0 49 70036.0 50 68854.0 51 67556.0 52 66568.5 53 67306.0 54 68085.5 55 65640.5 56 61077.0 57 56073.0 58 52208.0 59 46867.0 60 38427.5 61 31946.0 62 26670.5 63 20266.5 64 14817.0 65 11304.5 66 8407.5 67 6366.5 68 4875.0 69 3511.5 70 2535.0 71 1706.0 72 1154.0 73 886.5 74 642.0 75 435.5 76 307.5 77 209.0 78 131.5 79 92.0 80 57.5 81 33.5 82 21.5 83 12.5 84 11.5 85 8.5 86 3.5 87 5.5 88 7.0 89 5.5 90 4.0 91 2.5 92 1.0 93 1.0 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.46101830719176423 2 0.0436535877887308 3 0.05741230838958222 4 0.06171190857734829 5 0.08922934977905113 6 0.0863966720082876 7 0.04977419511484485 8 0.04309716894090225 9 0.04977419511484485 10-11 0.05414967060004208 12-13 0.04850960682432542 14-15 0.041554371226468545 16-17 0.042667208922125645 18-19 0.05111465870279544 20-21 0.06027027792615614 22-23 0.05141815989252012 24-25 0.06998231599734538 26-27 0.06070023794493275 28-29 0.048104938571359196 30-31 0.04964773628579291 32-33 0.04924306803282669 34-35 0.053492084688971986 36-37 0.04850960682432542 38-39 0.029566074232344322 40-41 0.027492149435892455 42-43 0.02668281292996002 44-45 0.01606027128959679 46-47 0.028453236536687222 48-49 0.028579695365739167 50-51 0.017855986662134383 52-53 0.01755248547240972 54-55 0.019095283186843427 56-57 0.017299567814305833 58-59 0.016363772479321453 60-61 0.01939878437656809 62-63 0.018892949060360317 64-65 0.0196011185030512 66-67 0.010850167532656727 68-69 0.014011638258955308 70-71 0.020941582091001797 72-73 0.01846298904158371 74-75 0.015984395992165622 76-77 0.011811254633451496 78-79 0.015175059486233187 80-81 0.01843769727577332 82-83 0.015756770099872128 84-85 0.014264555917059194 86-87 0.004350183719386847 88-89 0.031235330775829975 90-91 0.03513026271062983 92-93 0.01884236552873954 94-95 0.035711973324268766 96-97 0.029717824827206658 98-99 0.026606937632528856 100-101 0.1509918418880202 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1976928.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.28107069809703 #Duplication Level Percentage of deduplicated Percentage of total 1 76.43238173129686 42.25263898111761 2 13.34004369732086 14.749037974945967 3 4.448276289232919 7.377164280892614 4 2.0154973326561856 4.456754021535705 5 1.0195293327770079 2.8180336562014743 6 0.6107608038878629 2.025810670761092 7 0.4083395610788203 1.5801413701379725 8 0.279683548406272 1.2368964810033416 9 0.20700753939193955 1.0299238578148426 >10 1.116574971039816 11.233251260097068 >50 0.07473951124185688 2.814683817149004 >100 0.04119123717606561 4.102838348720517 >500 0.0032170085735295714 1.2174904382556981 >1k 0.0025736068588236554 2.2544481972775356 >5k 1.8382906134454678E-4 0.8508866440895583 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9308 0.47083151232619497 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7438 0.3762403081953415 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4635 0.23445466906230275 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2507 0.12681291377328865 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2407 0.12175456061121093 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2340 0.11836546399261885 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2219 0.1122448566665048 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2092 0.10582074815066607 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.058353162077729E-5 2 0.0 0.0 0.0 0.0 5.058353162077729E-5 3 0.0 0.0 0.0 0.0 5.058353162077729E-5 4 0.0 0.0 0.0 0.0 1.0116706324155457E-4 5 0.0 0.0 0.0 0.0 1.0116706324155457E-4 6 0.0 0.0 0.0 0.0 1.0116706324155457E-4 7 0.0 0.0 0.0 5.058353162077729E-5 1.0116706324155457E-4 8 0.0 0.0 0.0 5.058353162077729E-5 1.0116706324155457E-4 9 0.0 0.0 0.0 1.0116706324155457E-4 1.0116706324155457E-4 10-11 0.0 0.0 0.0 1.5175059486233187E-4 1.2645882905194323E-4 12-13 0.0 0.0 0.0 2.5291765810388645E-4 3.54084721345441E-4 14-15 0.0 0.0 0.0 2.7820942391427507E-4 4.2996001877660696E-4 16-17 0.0 0.0 0.0 3.0350118972466373E-4 5.058353162077729E-4 18-19 0.0 0.0 0.0 3.0350118972466373E-4 5.058353162077729E-4 20-21 0.0 0.0 0.0 3.0350118972466373E-4 5.311270820181615E-4 22-23 0.0 0.0 0.0 4.2996001877660696E-4 6.070023794493275E-4 24-25 0.0 0.0 0.0 8.852118033636025E-4 6.322941452597161E-4 26-27 0.0 0.0 0.0 0.0016692565434856506 7.08169442690882E-4 28-29 0.0 0.0 0.0 0.00447664254843879 7.08169442690882E-4 30-31 0.0 0.0 0.0 0.012544715841952766 7.08169442690882E-4 32-33 0.0 5.058353162077729E-5 0.0 0.030805370757053366 7.08169442690882E-4 34-35 0.0 5.058353162077729E-5 0.0 0.059410357888602924 9.105035691739911E-4 36-37 0.0 5.058353162077729E-5 0.0 0.10448028456271549 9.357953349843798E-4 38-39 0.0 5.058353162077729E-5 0.0 0.17221163340293627 0.0010116706324155458 40-41 0.0 5.058353162077729E-5 0.0 0.24629121546156463 0.0010116706324155458 42-43 0.0 5.058353162077729E-5 0.0 0.3222170964243513 0.0010116706324155458 44-45 0.0 5.058353162077729E-5 0.0 0.41367212159471667 0.001062254164036323 46-47 0.0 5.058353162077729E-5 0.0 0.5166096084429984 0.0011381294614674889 48-49 0.0 5.058353162077729E-5 0.0 0.6265529144207578 0.0011634212272778775 50-51 0.0 5.058353162077729E-5 0.0 0.7444884183946001 0.0013657553537609868 52-53 0.0 5.058353162077729E-5 0.0 0.851270253646061 0.001416338885381764 54-55 0.0 5.058353162077729E-5 0.0 0.966322496317519 0.00149221418281293 56-57 0.0 5.058353162077729E-5 0.0 1.1038085352627915 0.0015175059486233185 58-59 0.0 5.058353162077729E-5 0.0 1.2490085627802328 0.0015175059486233185 60-61 0.0 5.058353162077729E-5 0.0 1.4044517554508813 0.0015175059486233185 62-63 0.0 5.058353162077729E-5 0.0 1.5556206396995744 0.001568089480244096 64-65 0.0 5.058353162077729E-5 0.0 1.7140229689700384 0.001568089480244096 66-67 0.0 5.058353162077729E-5 0.0 1.8927092944204342 0.001568089480244096 68-69 0.0 5.058353162077729E-5 0.0 2.0875064746920473 0.001568089480244096 70-71 0.0 5.058353162077729E-5 0.0 2.2911304812314865 0.0016186730118648732 72-73 0.0 5.058353162077729E-5 0.0 2.498573544408294 0.0018210071383479823 74-75 0.0 5.058353162077729E-5 0.0 2.703892099257029 0.0018715906699687595 76-77 0.0 5.058353162077729E-5 0.0 2.9232475841305297 0.0018968824357791484 78-79 0.0 5.058353162077729E-5 0.0 3.167034914776866 0.001922174201589537 80-81 0.0 5.058353162077729E-5 0.0 3.4274642273264377 0.0019474659673999256 82-83 0.0 5.058353162077729E-5 0.0 3.6975297026497675 0.002073924796451869 84-85 0.0 5.058353162077729E-5 0.0 3.964711916670714 0.002073924796451869 86-87 0.0 1.0116706324155457E-4 0.0 4.241960253484194 0.0020992165622622577 88-89 0.0 1.0116706324155457E-4 0.0 4.535724113371858 0.002124508328072646 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4210 0.0 50.888947 1 GTATCAA 7550 0.0 42.34353 1 ATCAACG 9700 0.0 32.48823 3 TATCAAC 9735 0.0 32.466618 2 TCAACGC 9720 0.0 32.421387 4 CAACGCA 9975 0.0 31.449617 5 AACGCAG 10335 0.0 30.439173 6 ACGCAGA 11765 0.0 26.490997 7 CGCAGAG 11825 0.0 26.23605 8 TACATGG 7115 0.0 24.114798 2 GCAGAGT 12850 0.0 23.9954 9 GTACATG 7405 0.0 23.519442 1 TGGTATC 2015 0.0 22.879578 2 ACATGGG 7225 0.0 22.762636 3 GAGTACT 7860 0.0 22.66247 12-13 TAAGGTG 1130 0.0 22.293678 5 AGAGTAC 11375 0.0 21.338701 10-11 GTACTTT 8375 0.0 21.242687 14-15 AGTACTT 8075 0.0 21.147297 12-13 CAGAGTA 12110 0.0 20.984959 10-11 >>END_MODULE