FastQCFastQC Report
Thu 26 May 2016
SRR938150_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938150_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1976928
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90170.4561117046245488No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74810.3784154000550349No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48620.24593713074021914No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA26210.13257943637805727No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA24270.12276623124362648No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA23890.12084405704203695No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC23790.12033822172582917No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA23260.11765729454992796No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42350.052.0429761
GTATCAA76650.041.7681541
TCAACGC98650.032.642714
TATCAAC97850.032.621662
ATCAACG97950.032.538213
CAACGCA102450.031.4783125
AACGCAG105650.030.6147866
TAAGGTG12100.029.8319635
TGGTATC19400.028.157472
ACGCAGA117600.027.1807447
GTAAGGT12200.026.862284
CGCAGAG118700.026.8088198
GTGGTAT22450.025.3899841
GCAGAGT130200.024.0396449
GTACATG73600.023.1693671
TACATGG70350.023.1594142
GAGTACT81000.022.69235412-13
AGTACTT83300.022.1513212-13
GTACTTT83900.022.02121414-15
ACATGGG71300.021.9170423
Produced by FastQC (version 0.11.5)