FastQCFastQC Report
Thu 26 May 2016
SRR938149_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938149_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1989255
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95480.4799786854877831No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77700.39059849038961825No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47950.24104501433953918No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA26160.13150651877210312No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA23850.11989413121997934No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA23720.11924062023219748No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA22440.11280605050634535No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC21570.10843255389580521No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39050.056.371361
GTATCAA75800.044.7897871
TATCAAC95750.035.1932222
ATCAACG96850.034.7935033
TCAACGC97800.034.455534
CAACGCA100300.033.64415
AACGCAG103950.032.645646
ACGCAGA117450.028.778397
CGCAGAG118950.028.2149478
GCAGAGT127550.026.0144089
TGGTATC17550.025.998342
GTGGTAT19750.025.6281571
GAGTACT80700.023.0010812-13
GTACCGT1050.00218921322.6324846
GTACTTT82900.022.2639514-15
TAAGGTG12950.022.0207945
CAGAGTA123050.022.00005510-11
AGAGTAC117950.021.37957810-11
GTACATG75300.021.3704431
AGTACTT83100.021.24997912-13
Produced by FastQC (version 0.11.5)