FastQCFastQC Report
Thu 26 May 2016
SRR938149_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938149_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1989255
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92160.4632890202613541No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77440.3892914684140545No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49840.2505460587003677No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA25940.1304005771004723No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA24810.12472005851436846No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC24610.12371465699470405No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA24180.12155304372742559No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA22600.11361037172207686No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44250.053.5722471
GTATCAA76950.040.5610581
ATCAACG95900.032.342293
TCAACGC97100.031.9409794
TATCAAC97300.031.9313642
CAACGCA99600.031.1392485
AACGCAG102350.030.3489886
ACGCAGA117350.026.30787
CGCAGAG117850.026.1961888
TAAGGTG12450.025.5599355
AATACCG1351.3443127E-524.6274015
TACATGG72900.023.9813062
GTACATG75750.023.9570471
GCAGAGT130050.023.4830749
ACATGGG73200.023.0352763
GAGTACT81200.022.63649712-13
GTACTTT83300.022.0943414-15
CAGAGTA125550.021.16594510-11
TGGTATC19100.021.1416132
GGTAAGG14650.021.0742383
Produced by FastQC (version 0.11.5)