##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938149_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1989255 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.518611741581644 34.0 31.0 34.0 31.0 34.0 2 32.73253705532976 34.0 31.0 34.0 31.0 34.0 3 32.79230566217001 34.0 31.0 34.0 31.0 34.0 4 36.24736144938683 37.0 35.0 37.0 35.0 37.0 5 36.21066831552516 37.0 37.0 37.0 35.0 37.0 6 36.251301617942396 37.0 37.0 37.0 35.0 37.0 7 36.21869343045512 37.0 37.0 37.0 35.0 37.0 8 36.210349100542665 37.0 37.0 37.0 35.0 37.0 9 37.924128379720045 39.0 38.0 39.0 35.0 39.0 10-11 37.91112602456698 39.0 38.0 39.0 35.0 39.0 12-13 37.86296327016898 39.0 38.0 39.0 35.0 39.0 14-15 39.194302640938446 40.5 39.0 41.0 36.0 41.0 16-17 39.15040253763344 40.0 39.0 41.0 36.0 41.0 18-19 39.18039818927186 40.0 39.0 41.0 36.0 41.0 20-21 39.112434051944064 40.0 39.0 41.0 36.0 41.0 22-23 38.97376178519094 40.0 38.5 41.0 35.0 41.0 24-25 38.93270998439114 40.0 38.0 41.0 35.0 41.0 26-27 38.8960977853518 40.0 38.0 41.0 35.0 41.0 28-29 38.841577625794585 40.0 38.0 41.0 35.0 41.0 30-31 38.64329309213751 40.0 38.0 41.0 35.0 41.0 32-33 38.46593196950617 40.0 38.0 41.0 34.0 41.0 34-35 38.269515974573395 40.0 38.0 41.0 34.0 41.0 36-37 38.13454660161719 40.0 38.0 41.0 34.0 41.0 38-39 38.12005549816388 40.0 38.0 41.0 34.0 41.0 40-41 38.05649124923652 40.0 38.0 41.0 33.5 41.0 42-43 37.9020937486647 40.0 38.0 41.0 33.0 41.0 44-45 37.69781149224207 40.0 37.0 41.0 33.0 41.0 46-47 37.38026421951937 40.0 37.0 41.0 32.5 41.0 48-49 37.039164662147385 40.0 37.0 41.0 31.5 41.0 50-51 36.74272453757814 40.0 36.0 41.0 31.0 41.0 52-53 36.33028545862647 39.0 35.5 41.0 30.0 41.0 54-55 36.10703303498043 39.0 35.0 41.0 30.0 41.0 56-57 35.858339428580045 39.0 35.0 40.5 29.0 41.0 58-59 35.52700131456248 38.5 35.0 40.0 28.5 41.0 60-61 35.176935083737376 38.0 35.0 40.0 28.0 41.0 62-63 34.81621486435877 37.5 34.0 40.0 27.5 41.0 64-65 34.43157362932354 37.0 34.0 40.0 26.0 41.0 66-67 34.024341524842214 36.0 34.0 39.0 26.0 41.0 68-69 33.57062417839845 36.0 33.0 39.0 25.5 40.5 70-71 33.06693561157318 35.0 33.0 38.5 24.5 40.0 72-73 32.66194329032729 35.0 32.5 37.5 24.0 40.0 74-75 32.21997204983775 35.0 32.0 37.0 22.5 39.0 76-77 30.908070860699105 34.0 30.5 35.5 21.0 38.5 78-79 31.625326818331487 35.0 32.0 36.0 22.0 38.5 80-81 31.469204300102298 35.0 32.0 36.0 21.5 37.5 82-83 31.10769006487353 35.0 31.5 35.0 20.0 37.0 84-85 30.732214321441948 34.0 31.0 35.0 18.5 36.5 86-87 30.314636635323275 34.0 31.0 35.0 16.0 36.0 88-89 29.86197671992781 34.0 30.0 35.0 9.0 36.0 90-91 29.536127595506862 34.0 30.0 35.0 6.0 35.0 92-93 29.149778183289726 34.0 30.0 35.0 2.0 35.0 94-95 28.74723703094877 34.0 29.0 35.0 2.0 35.0 96-97 28.18630492319989 34.0 29.0 35.0 2.0 35.0 98-99 27.512802531601025 34.0 28.0 35.0 2.0 35.0 100-101 25.073716290772175 31.0 22.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0747368379910327 1101 2 0.13795469418013795 1101 3 0.17975520323907546 1101 4 0.08928024826126801 1101 5 0.09756770640024115 1101 6 0.0487596640984691 1101 7 0.08183138467310158 1101 8 0.08909845076895806 1101 9 -0.029694057140211783 1101 10-11 0.05862202361230118 1101 12-13 0.1633586665615212 1101 14-15 0.17967680381889295 1101 16-17 0.16826334741912774 1101 18-19 0.2861480901818396 1101 20-21 0.24983174525702623 1101 22-23 0.18294080771600107 1101 24-25 0.19795782366141879 1101 26-27 0.2217118858210867 1101 28-29 0.2225709685318762 1101 30-31 0.1006871215156977 1101 32-33 0.1821912135878705 1101 34-35 0.09292209735475154 1101 36-37 0.11478823213447953 1101 38-39 0.17294300313741218 1101 40-41 0.19009741233475808 1101 42-43 0.2951364079016301 1101 44-45 0.22935880751773396 1101 46-47 0.1728167486427452 1101 48-49 0.1651330812485483 1101 50-51 0.29445456307932005 1101 52-53 0.14259634456468007 1101 54-55 0.1682895983880286 1101 56-57 0.17727441482540485 1101 58-59 0.1284167471602231 1101 60-61 0.03335651921771898 1101 62-63 -0.07807747712887902 1101 64-65 0.08874828130034018 1101 66-67 -0.012082508299627648 1101 68-69 0.13926605576934037 1101 70-71 0.2940156646290859 1101 72-73 0.06912307045585919 1101 74-75 0.2092653993405591 1101 76-77 0.07859747986452703 1101 78-79 0.06355791381688292 1101 80-81 0.32518430311199253 1101 82-83 0.39281240494343805 1101 84-85 0.5833138839119343 1101 86-87 0.5682473875381255 1101 88-89 0.38748713325210105 1101 90-91 0.4081342091338698 1101 92-93 0.4966615817311322 1101 94-95 0.49722941171340906 1101 96-97 0.6314801695702066 1101 98-99 0.44957046384719135 1101 100-101 0.7827845694871378 1102 1 0.16631157421070952 1102 2 0.13288697054431253 1102 3 0.10968809995227957 1102 4 0.06374826596893257 1102 5 0.08356563681027751 1102 6 0.03768313414617097 1102 7 0.08773129993605977 1102 8 0.03288294943454417 1102 9 0.09034480046724269 1102 10-11 0.04231113371501749 1102 12-13 0.13637348366414415 1102 14-15 0.20590469893502927 1102 16-17 0.17690434747456862 1102 18-19 0.11453117022797699 1102 20-21 0.1792878311450039 1102 22-23 0.15985689429032846 1102 24-25 0.04686455748416307 1102 26-27 0.01580451317082776 1102 28-29 0.013282033710758867 1102 30-31 0.0081521641877913 1102 32-33 -0.09203407606233327 1102 34-35 0.09226809108789524 1102 36-37 -0.003492199945220875 1102 38-39 0.04770080303656954 1102 40-41 0.15708131493732225 1102 42-43 0.108180368209851 1102 44-45 0.16115407121887415 1102 46-47 0.17109429015994237 1102 48-49 0.11600572755192928 1102 50-51 0.12385098986889886 1102 52-53 0.22333081142088673 1102 54-55 0.2362544443962804 1102 56-57 0.32630502047084775 1102 58-59 0.2816066610768999 1102 60-61 0.20242769832964314 1102 62-63 0.14748077100545487 1102 64-65 0.15981322260476105 1102 66-67 0.007827725875529268 1102 68-69 0.2935775475371827 1102 70-71 0.30125790960624244 1102 72-73 0.15456988014597783 1102 74-75 0.3215304256459959 1102 76-77 0.14468148040304385 1102 78-79 0.2225992369817753 1102 80-81 0.3353741959103971 1102 82-83 0.3830290387448052 1102 84-85 0.20697881360131376 1102 86-87 0.2863310897811502 1102 88-89 0.28809401939033563 1102 90-91 0.2492951593502042 1102 92-93 0.30688592369919476 1102 94-95 0.25269640254544257 1102 96-97 0.2959234789079126 1102 98-99 0.2580393500435001 1102 100-101 0.4226828454447862 1103 1 0.033193892531876656 1103 2 0.06811022051576288 1103 3 0.03187311489166689 1103 4 0.026175184716514366 1103 5 0.08570238894702698 1103 6 0.04924123460457963 1103 7 0.0025655236089292544 1103 8 -0.03951501916075273 1103 9 0.08555365692995309 1103 10-11 0.04475392045893045 1103 12-13 0.03742250316126672 1103 14-15 0.07797297837740302 1103 16-17 0.07612892385741787 1103 18-19 0.15527816446932263 1103 20-21 0.11108854442053939 1103 22-23 0.08172112974654766 1103 24-25 0.05235812181637556 1103 26-27 0.057626157895860786 1103 28-29 0.23249583064809798 1103 30-31 0.1188758301529731 1103 32-33 0.0920252698675128 1103 34-35 0.12246089132180771 1103 36-37 0.04343462280380095 1103 38-39 0.01252188180934155 1103 40-41 0.09706072858066506 1103 42-43 0.03870188506230221 1103 44-45 0.11273660425053578 1103 46-47 0.1837293940077842 1103 48-49 0.29044529677567965 1103 50-51 0.3680021367692774 1103 52-53 0.2897473798929511 1103 54-55 0.3978402463983244 1103 56-57 0.3804000490006132 1103 58-59 0.42481485747111947 1103 60-61 0.339009641566264 1103 62-63 0.5127678192318612 1103 64-65 0.4116340579302147 1103 66-67 0.38494628220786353 1103 68-69 0.36792136918315066 1103 70-71 0.31410665729568876 1103 72-73 0.30392621925051344 1103 74-75 0.21011547597308322 1103 76-77 0.30536564892380014 1103 78-79 0.29228618757828073 1103 80-81 0.22473884234081964 1103 82-83 0.39401004985428756 1103 84-85 0.4891739366237715 1103 86-87 0.46874026448220363 1103 88-89 0.23405216653039318 1103 90-91 0.21892322455894586 1103 92-93 0.27008475239134455 1103 94-95 0.14952117192930459 1103 96-97 0.12138587194482042 1103 98-99 0.2771297292315147 1103 100-101 0.4587364201674262 1104 1 0.08904585623749028 1104 2 0.020933600671199315 1104 3 0.1615591475617535 1104 4 0.08222408290605898 1104 5 0.04992138009315994 1104 6 0.041564588170935224 1104 7 0.1363021538320055 1104 8 0.08582852048239431 1104 9 0.05549192936456393 1104 10-11 0.09656858115016576 1104 12-13 0.12558574723905735 1104 14-15 0.19722440527584695 1104 16-17 0.0941932799430063 1104 18-19 0.11720640434838003 1104 20-21 0.22415172883361123 1104 22-23 0.2352372576289028 1104 24-25 0.1731997800631646 1104 26-27 0.18411229738976687 1104 28-29 0.1600430788539171 1104 30-31 0.1391177147867637 1104 32-33 0.14760739826763114 1104 34-35 0.3020258414249213 1104 36-37 0.24636143739144956 1104 38-39 0.2753121045089344 1104 40-41 0.23093220340957288 1104 42-43 0.2073406236531028 1104 44-45 0.2530937686043373 1104 46-47 0.26845329712068633 1104 48-49 0.4295920737412402 1104 50-51 0.4385908773761713 1104 52-53 0.3352315027076074 1104 54-55 0.374612057768978 1104 56-57 0.43692316776495943 1104 58-59 0.3362349537878586 1104 60-61 0.42389775414500264 1104 62-63 0.4332249877347323 1104 64-65 0.3643911114864693 1104 66-67 0.28305328600454516 1104 68-69 0.4454292597109628 1104 70-71 0.46452501158958626 1104 72-73 0.3147058986505229 1104 74-75 0.2861127562903292 1104 76-77 0.2610811025539128 1104 78-79 0.36139324455312405 1104 80-81 0.2788204410047008 1104 82-83 0.24838167399109068 1104 84-85 0.43601243690632074 1104 86-87 0.25422528723485627 1104 88-89 0.2943442139419332 1104 90-91 0.3667815766555975 1104 92-93 0.35994253881268534 1104 94-95 0.029428390659237635 1104 96-97 0.41725363715917396 1104 98-99 0.5793909987635324 1104 100-101 0.6113550936471306 1105 1 0.09903337957402414 1105 2 0.08172462221215682 1105 3 0.09669638278266746 1105 4 0.08024165577769082 1105 5 0.04930023933733452 1105 6 0.09526458930174897 1105 7 0.05476013084629727 1105 8 0.04278349302781237 1105 9 0.054855711069798474 1105 10-11 0.1018154300791494 1105 12-13 0.11409639332775612 1105 14-15 0.18257172984733927 1105 16-17 0.05544360101381329 1105 18-19 0.0772824274536319 1105 20-21 0.020475717144215366 1105 22-23 0.07487188039696235 1105 24-25 0.1652556671151686 1105 26-27 0.14419922478656844 1105 28-29 0.05365071390423992 1105 30-31 0.13625244390532032 1105 32-33 0.02309183151640326 1105 34-35 0.09246380085234307 1105 36-37 0.05158130829541108 1105 38-39 0.06745867218797486 1105 40-41 0.01088077284729394 1105 42-43 -0.09063704758870017 1105 44-45 -0.14192577027067443 1105 46-47 -0.02247885556954543 1105 48-49 -0.13093966797836742 1105 50-51 -0.1509710501148689 1105 52-53 -0.17445029660281364 1105 54-55 -0.06295037266568215 1105 56-57 -0.09769324618240915 1105 58-59 -0.06874128024139026 1105 60-61 -0.13793254061549476 1105 62-63 -0.24708514647151958 1105 64-65 -0.13523760411722918 1105 66-67 -0.18403823519527407 1105 68-69 -0.19031368871254983 1105 70-71 -0.10606412170501045 1105 72-73 -0.17512403209510552 1105 74-75 -0.14880248909392435 1105 76-77 -0.2968150315112581 1105 78-79 -0.2453918095133183 1105 80-81 -0.19784168265588775 1105 82-83 -0.08096050913962216 1105 84-85 0.02210087636488467 1105 86-87 0.10134402339246407 1105 88-89 -0.21809960203239598 1105 90-91 -0.34371352125955923 1105 92-93 -0.2835217249968629 1105 94-95 -0.4511117567883254 1105 96-97 -0.32256996640493796 1105 98-99 -0.1926173617318092 1105 100-101 0.11114050795905328 1106 1 -0.14693962409360495 1106 2 -0.12633816614668092 1106 3 -0.01889864076322567 1106 4 0.0036437992960713927 1106 5 -0.07429138561427351 1106 6 -0.04171119313456728 1106 7 -0.022449039270938442 1106 8 -0.16570695693771853 1106 9 -0.2046531177693467 1106 10-11 -0.1407803881744769 1106 12-13 -0.21592024110952934 1106 14-15 -0.3143370092808837 1106 16-17 -0.2069113183972533 1106 18-19 -0.14379244247456313 1106 20-21 -0.06327244536822718 1106 22-23 -0.12853228535725236 1106 24-25 -0.21558473179148763 1106 26-27 -0.08346764384574357 1106 28-29 -0.020667484386812873 1106 30-31 -0.022572771208729137 1106 32-33 -0.05420619832550244 1106 34-35 0.011377818944644957 1106 36-37 -0.05163641969684818 1106 38-39 -0.036994708956790134 1106 40-41 0.12735123878951526 1106 42-43 -0.021381016283250176 1106 44-45 0.11327265259173913 1106 46-47 0.2987382157119498 1106 48-49 0.031772160579372155 1106 50-51 0.06834145676886294 1106 52-53 0.09828392873254899 1106 54-55 -0.12325878426392478 1106 56-57 -0.043080239956033495 1106 58-59 0.13295585022144252 1106 60-61 0.0061255811246496705 1106 62-63 0.0065660338409827546 1106 64-65 -0.14190569460299685 1106 66-67 -0.06833255614319 1106 68-69 -0.2858266724209102 1106 70-71 -0.1299819611523816 1106 72-73 0.001135744731563193 1106 74-75 -0.16730762348361594 1106 76-77 -0.2068625514737441 1106 78-79 -0.1874079373598896 1106 80-81 -0.27609434193468374 1106 82-83 -0.14640253661674762 1106 84-85 -0.06610510383010038 1106 86-87 0.08697041480807144 1106 88-89 0.05599202330611419 1106 90-91 0.054433501991255184 1106 92-93 0.09167330855703781 1106 94-95 -0.022758746636132088 1106 96-97 0.10124333067259528 1106 98-99 0.048216093477343236 1106 100-101 0.09109277390782822 1107 1 0.09556837610902136 1107 2 0.1168584497988121 1107 3 0.07914908221650307 1107 4 0.07250096879172219 1107 5 -0.0018641325817583265 1107 6 0.05490056780565311 1107 7 0.08584055815313718 1107 8 0.02549770215711078 1107 9 0.049377082949661144 1107 10-11 0.05558705366209438 1107 12-13 0.08307480570239534 1107 14-15 0.06340176162076716 1107 16-17 0.07704430185980016 1107 18-19 0.11151404659091213 1107 20-21 0.11891450426224992 1107 22-23 0.2096147509888837 1107 24-25 0.07709421102918412 1107 26-27 -0.004697858072781003 1107 28-29 0.002179068092978298 1107 30-31 0.0945939494166339 1107 32-33 0.06779037621495121 1107 34-35 0.0860372000861176 1107 36-37 -0.034821495088529275 1107 38-39 -0.019002970877444625 1107 40-41 -0.04064971264545392 1107 42-43 -0.10949189663222825 1107 44-45 0.02292340401246662 1107 46-47 -0.13242406928834782 1107 48-49 -0.02788523473525828 1107 50-51 -0.0745448416131893 1107 52-53 0.01960297292215074 1107 54-55 0.04955109832635429 1107 56-57 0.10292391669890577 1107 58-59 0.12495895119468514 1107 60-61 -0.01758577875930456 1107 62-63 0.11166692926169475 1107 64-65 0.02748680389019853 1107 66-67 0.018716769446513126 1107 68-69 0.009858907686464136 1107 70-71 0.08798914781996103 1107 72-73 0.1872172642084493 1107 74-75 0.2554980378481133 1107 76-77 0.10877343068098 1107 78-79 0.18373906301378184 1107 80-81 0.2429002203501689 1107 82-83 0.16933695813897742 1107 84-85 0.2667301209941293 1107 86-87 0.30205271466719097 1107 88-89 0.2339199065908879 1107 90-91 0.20437811173773568 1107 92-93 0.22245377154467505 1107 94-95 0.21535877760673827 1107 96-97 0.4443072155665817 1107 98-99 0.6360153348254158 1107 100-101 0.839392707909365 1108 1 0.05251080474955927 1108 2 0.0486436457349555 1108 3 0.084954597455976 1108 4 0.017348574016679663 1108 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-0.6582452204540381 2316 100-101 -0.7837602370007026 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 6.0 4 3.0 5 18.0 6 44.0 7 165.0 8 390.0 9 755.0 10 1300.0 11 2095.0 12 3341.0 13 4827.0 14 6477.0 15 8970.0 16 11597.0 17 16731.0 18 16734.0 19 12149.0 20 10298.0 21 9899.0 22 9943.0 23 10872.0 24 12455.0 25 14336.0 26 16649.0 27 20602.0 28 25425.0 29 30921.0 30 38344.0 31 47626.0 32 59678.0 33 78679.0 34 110266.0 35 168882.0 36 284951.0 37 476708.0 38 426563.0 39 50552.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.986796564543006 19.08181706216649 14.979276161175918 22.952110212114583 2 15.736909874049818 20.008285733103868 39.734967151191 24.519837241655317 3 19.3304771260428 23.80879364622913 30.11098386319967 26.749745364528398 4 13.084948887900245 16.87199479202013 36.686498211642046 33.356558108437575 5 13.629323540722533 37.3055741973754 34.2633297390229 14.801772522879169 6 32.35392144295226 36.36853998104818 16.89682820955584 14.380710366443719 7 28.641677411895405 33.01673239479101 20.06258624459911 18.279003948714468 8 26.804564742337178 34.576400014678924 19.295994916669475 19.32304032631443 9 26.214582022114936 16.7236397344921 20.1663546553364 36.895423588056566 10-11 25.123727224513697 26.57881970888599 26.82690253386318 21.470550532737132 12-13 26.013381894226733 23.839175972914482 27.448215537977788 22.699226594880997 14-15 22.662127278805382 25.30344274615371 25.899143146554866 26.13528682848604 16-17 22.036819944019044 28.481767984685668 26.9147939321794 22.56661813911589 18-19 22.08942543816655 26.67528798469779 29.421818721078996 21.813467856056665 20-21 22.542132607433434 25.826050456075265 29.8702780689253 21.761538867566 22-23 22.287564942654413 25.813005371357615 29.814377744431958 22.08505194155601 24-25 22.123466708158563 26.084945605985695 29.827008458665755 21.964579227189994 26-27 21.725823547039575 26.391467169741496 29.59904687968239 22.283662403536542 28-29 21.80862227532978 26.786718340966427 29.429693385134986 21.97496599856881 30-31 21.994942830356088 26.359064071725346 29.33598256633765 22.310010531580918 32-33 21.6456528525618 26.26179654914649 29.404367582306268 22.688183015985448 34-35 22.430541850868142 26.456053728799283 29.17012234287934 21.943282077453237 36-37 22.554446048127584 25.961820275883774 29.23056891539239 22.25316476059625 38-39 21.934402384033174 26.45823806493896 29.129867612650923 22.477491938376946 40-41 22.46041989179166 26.228254131218577 29.221555591643995 22.08977038534577 42-43 22.234047848034955 26.299175150030997 28.96856299542152 22.49821400651253 44-45 22.18716058828808 26.53788931253866 29.16583613827801 22.109113960895247 46-47 22.576227068036562 26.730824721227155 28.430079818866773 22.262868391869514 48-49 22.273263106037184 26.82237822702469 28.344003156960774 22.560355509977352 50-51 22.369505166507057 27.09210231971266 28.305169523263736 22.23322299051655 52-53 22.735270239360965 26.772309231345403 28.216945539913183 22.27547498938045 54-55 22.323910207590277 27.050327886570603 28.322939995123804 22.302821910715316 56-57 22.379810532083617 27.130885683333712 28.135709097124302 22.35359468745837 58-59 22.341932029830264 26.779221366793095 28.075410140982427 22.803436462394213 60-61 22.053256118496623 27.052137609305998 28.397515652844906 22.497090619352473 62-63 22.119562348718492 27.000007540511394 28.521079499611663 22.359350611158447 64-65 21.83262075500627 27.096903111969056 28.376100600476057 22.694375532548616 66-67 21.904004262902443 27.086849096772408 28.178740282165936 22.830406358159212 68-69 22.363475727691686 26.75771426704252 28.361180895839016 22.51762910942678 70-71 22.102495657922187 26.91246220318662 27.995882880776975 22.98915925811422 72-73 22.133436889689857 27.0608091974131 27.947296852339193 22.858457060557846 74-75 22.139946864529687 27.429967500395875 27.736866314273435 22.693219320801003 76-77 22.139016868123996 27.01735071672561 28.050425913218767 22.79320650193163 78-79 22.257930732862302 27.350113484696532 27.83632565960624 22.55563012283493 80-81 22.334517193622737 27.279936458623954 27.661637145564544 22.723909202188757 82-83 22.314470650806783 27.444609888933236 27.749673998393366 22.491245461866612 84-85 22.28490576898376 27.503009405129397 27.6090329660883 22.603051859798544 86-87 22.398107019551148 27.432280806175612 27.390078844023158 22.779533330250086 88-89 22.210964512324722 27.63189115115516 27.24081489844937 22.916329438070754 90-91 22.110452097183288 27.621670764103985 27.41262228531686 22.855254853395863 92-93 22.395453557186826 27.230975532205015 27.5362417195447 22.837329191063453 94-95 22.044685070541483 27.158308009782555 27.663673083641864 23.133333836034094 96-97 22.3009675232606 26.727449906485067 27.829194303695175 23.142388266559156 98-99 22.057529074955195 27.026022304832715 27.4281075075845 23.48834111262759 100-101 22.10877941742009 26.869003722499123 27.69026595383699 23.331950906243794 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 669.0 1 459.5 2 359.5 3 494.5 4 407.5 5 374.5 6 774.0 7 1801.0 8 3020.5 9 3519.0 10 2890.0 11 2107.5 12 2005.5 13 2150.0 14 2249.0 15 2335.5 16 2496.0 17 2707.5 18 2955.5 19 3245.5 20 3644.0 21 4380.0 22 5339.5 23 6460.5 24 7936.0 25 9787.0 26 12735.0 27 16539.0 28 19051.5 29 21194.0 30 23860.0 31 27653.0 32 34006.0 33 36809.5 34 36967.5 35 39496.5 36 43629.5 37 47991.0 38 50729.0 39 52858.0 40 56128.0 41 59950.5 42 61378.5 43 62253.0 44 64596.5 45 68665.0 46 70892.0 47 71952.0 48 72879.0 49 71643.5 50 70518.5 51 68891.0 52 67761.5 53 68529.5 54 69779.0 55 66796.0 56 61343.0 57 56350.0 58 52503.0 59 46954.0 60 37568.0 61 30572.0 62 25732.0 63 18947.0 64 13035.0 65 9856.5 66 7300.5 67 5565.0 68 4189.5 69 2875.5 70 1963.5 71 1244.0 72 836.5 73 675.5 74 463.0 75 326.0 76 228.5 77 135.5 78 100.5 79 56.0 80 25.0 81 16.0 82 9.0 83 6.0 84 5.5 85 4.0 86 1.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.014779402339066636 3 0.006887000409701119 4 0.0 5 0.0 6 0.0 7 0.0 8 4.021606078657588E-4 9 5.529708358154184E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.5189053188253893E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005605113472129013 26-27 0.021063161836969116 28-29 2.5135037991609925E-5 30-31 0.0 32-33 4.021606078657588E-4 34-35 0.018976953683665494 36-37 0.004222686382590467 38-39 0.017921282088017878 40-41 0.015784803858731034 42-43 0.008344832613214495 44-45 0.002991069521001581 46-47 5.027007598321985E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.2567518995804962E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.027007598321985E-5 84-85 0.0037451206607498786 86-87 5.529708358154184E-4 88-89 5.027007598321985E-5 90-91 1.7594526594126946E-4 92-93 3.7702556987414886E-4 94-95 0.0 96-97 2.5135037991609925E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1989255.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.24693516318519 #Duplication Level Percentage of deduplicated Percentage of total 1 74.7666120873301 39.81092946185084 2 14.205022682313148 15.127478435134064 3 4.806259969519773 7.677558389232954 4 2.106587031039608 4.486772122294912 5 1.1501511570802576 3.062101204445745 6 0.6641686155506878 2.121896592578997 7 0.4285392467498106 1.5972881040597173 8 0.3046021425901488 1.2975304429651955 9 0.22120333217578284 1.060055953761998 >10 1.2112794398604436 11.800740848909333 >50 0.08480669001801218 3.078719521683296 >100 0.04406946123616697 4.275056816663232 >500 0.003773602555457202 1.356346434756552 >1k 0.002735861852706466 2.3955707243104 >5k 1.886801277728597E-4 0.8519549473527309 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9216 0.4632890202613541 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7744 0.3892914684140545 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4984 0.2505460587003677 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2594 0.1304005771004723 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2481 0.12472005851436846 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2461 0.12371465699470405 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2418 0.12155304372742559 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2260 0.11361037172207686 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.027007598321985E-5 2 0.0 0.0 0.0 0.0 5.027007598321985E-5 3 0.0 0.0 0.0 0.0 1.005401519664397E-4 4 0.0 0.0 0.0 0.0 1.005401519664397E-4 5 0.0 0.0 0.0 0.0 1.5081022794965956E-4 6 0.0 0.0 0.0 0.0 2.010803039328794E-4 7 0.0 0.0 0.0 0.0 2.5135037991609924E-4 8 0.0 0.0 0.0 0.0 2.5135037991609924E-4 9 0.0 0.0 0.0 0.0 2.5135037991609924E-4 10-11 0.0 0.0 0.0 5.027007598321985E-5 3.016204558993191E-4 12-13 0.0 0.0 0.0 5.027007598321985E-5 4.021606078657588E-4 14-15 0.0 0.0 0.0 7.540511397482978E-5 4.524306838489786E-4 16-17 0.0 0.0 0.0 1.005401519664397E-4 5.278357978238085E-4 18-19 0.0 0.0 0.0 1.5081022794965956E-4 6.032409117986382E-4 20-21 0.0 0.0 0.0 2.764854179077092E-4 7.289161017566877E-4 22-23 0.0 0.0 0.0 5.529708358154184E-4 7.791861777399077E-4 24-25 0.0 0.0 0.0 6.78646025773468E-4 8.043212157315176E-4 26-27 0.0 0.0 0.0 0.0011310767096224467 9.80266481672787E-4 28-29 0.0 0.0 0.0 0.0032926899769009 0.001005401519664397 30-31 0.0 0.0 0.0 0.012240763501914033 0.001055671595647617 32-33 0.0 0.0 0.0 0.03202203840131104 0.0011059416716308367 34-35 0.0 0.0 0.0 0.06175678834538559 0.0014578322035133757 36-37 0.0 0.0 0.0 0.10803039328793945 0.0014578322035133757 38-39 0.0 0.0 0.0 0.17581959075131143 0.0015081022794965955 40-41 0.0 0.0 0.0 0.24853525566103893 0.0015081022794965955 42-43 0.0 0.0 0.0 0.325146851459466 0.0015081022794965955 44-45 0.0 0.0 0.0 0.4156832583052449 0.0015332373174882052 46-47 0.0 0.0 0.0 0.5230601406054025 0.0015835073934714252 48-49 0.0 0.0 0.0 0.6324478259448889 0.0016086424314630352 50-51 0.0 0.0 0.0 0.7468625188826973 0.0017594526594126947 52-53 0.0 0.0 0.0 0.8552448027025192 0.0018097227353959145 54-55 0.0 0.0 0.0 0.9755159594923728 0.0018097227353959145 56-57 0.0 0.0 0.0 1.1156437962955983 0.0018097227353959145 58-59 0.0 0.0 0.0 1.261477286722919 0.0018097227353959145 60-61 0.0 0.0 0.0 1.4179680332586821 0.0018097227353959145 62-63 0.0 0.0 0.0 1.5726741920970415 0.001960532963345574 64-65 0.0 0.0 0.0 1.7340914060791603 0.002061073115312014 66-67 0.0 0.0 0.0 1.9139074678711379 0.0020862081533036237 68-69 0.0 0.0 0.0 2.111016435801343 0.0021113431912952335 70-71 0.0 0.0 0.0 2.3155402399390725 0.0021364782292868437 72-73 0.0 0.0 0.0 2.5271269897524453 0.0022118833432616734 74-75 0.0 0.0 0.0 2.7349686189050675 0.002312423495228113 76-77 0.0 0.0 0.0 2.961309636019515 0.002362693571211333 78-79 0.0 0.0 0.0 3.21587730079854 0.002362693571211333 80-81 0.0 0.0 0.0 3.4787646631527886 0.002412963647194553 82-83 0.0 0.0 0.0 3.756180077466187 0.002614043951127432 84-85 0.0 0.0 0.0 4.029473345548961 0.0026391789891190417 86-87 0.0 0.0 0.0 4.318953578098334 0.0027899892170687017 88-89 0.0 0.0 0.0 4.626531038001664 0.0029156644070267514 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4425 0.0 53.572247 1 GTATCAA 7695 0.0 40.561058 1 ATCAACG 9590 0.0 32.34229 3 TCAACGC 9710 0.0 31.940979 4 TATCAAC 9730 0.0 31.931364 2 CAACGCA 9960 0.0 31.139248 5 AACGCAG 10235 0.0 30.348988 6 ACGCAGA 11735 0.0 26.3078 7 CGCAGAG 11785 0.0 26.196188 8 TAAGGTG 1245 0.0 25.559935 5 AATACCG 135 1.3443127E-5 24.627401 5 TACATGG 7290 0.0 23.981306 2 GTACATG 7575 0.0 23.957047 1 GCAGAGT 13005 0.0 23.483074 9 ACATGGG 7320 0.0 23.035276 3 GAGTACT 8120 0.0 22.636497 12-13 GTACTTT 8330 0.0 22.09434 14-15 CAGAGTA 12555 0.0 21.165945 10-11 TGGTATC 1910 0.0 21.141613 2 GGTAAGG 1465 0.0 21.074238 3 >>END_MODULE