FastQCFastQC Report
Thu 26 May 2016
SRR938148_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938148_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1985019
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90080.45379918277860315No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71070.3580318374786337No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45380.22861242134206272No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA25570.1288148879179494No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA25040.12614488828570405No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA22940.11556564445982632No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA22660.11415507861637596No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC21870.11017526784378386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43000.055.250961
GTATCAA75350.041.134431
TATCAAC96400.032.0664062
ATCAACG96350.031.9357763
TCAACGC96800.031.8364434
CAACGCA99900.030.8961355
AACGCAG102900.030.0362826
TGGTATC18150.028.560372
ACGCAGA117350.026.0488367
CGCAGAG118100.025.8002998
GTGGTAT20450.025.3770391
GCAGAGT129900.023.0906859
GAGTACT79700.022.99231112-13
TACATGG69400.022.0653252
GTACTTT83700.022.03410714-15
GTACATG71950.021.4398611
CAGAGTA124100.020.7985810-11
AGAGTAC115000.020.79101810-11
GTGTAGC13250.020.4817351
GGTACGC1404.8193082E-420.378466
Produced by FastQC (version 0.11.5)