##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938148_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1985019 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.977553363469067 33.0 31.0 34.0 30.0 34.0 2 32.0954958113751 34.0 31.0 34.0 30.0 34.0 3 32.120162577788925 34.0 31.0 34.0 30.0 34.0 4 35.63514807666829 37.0 35.0 37.0 33.0 37.0 5 35.64474999987406 37.0 35.0 37.0 35.0 37.0 6 35.675636354110466 37.0 35.0 37.0 35.0 37.0 7 35.60699721262114 37.0 35.0 37.0 33.0 37.0 8 35.5967534819566 37.0 35.0 37.0 33.0 37.0 9 37.23562595622511 39.0 37.0 39.0 34.0 39.0 10-11 37.148368605036026 39.0 37.0 39.0 34.0 39.0 12-13 36.99887255487227 39.0 37.0 39.0 33.0 39.0 14-15 38.185532984822814 40.0 38.0 41.0 33.0 41.0 16-17 38.00621480197419 40.0 38.0 41.0 33.0 41.0 18-19 38.18914529281584 40.0 38.0 41.0 33.5 41.0 20-21 38.19102537557575 40.0 38.0 41.0 33.5 41.0 22-23 38.07850277503641 40.0 38.0 41.0 33.0 41.0 24-25 37.95899082074277 40.0 38.0 41.0 33.0 41.0 26-27 37.77892831252497 40.0 38.0 41.0 32.5 41.0 28-29 37.60164789354661 40.0 37.5 41.0 32.0 41.0 30-31 37.373058393899505 40.0 37.0 41.0 31.5 41.0 32-33 37.075029004760154 40.0 36.5 41.0 31.0 41.0 34-35 36.835582933966876 39.5 36.0 41.0 30.5 41.0 36-37 36.56301954792372 39.0 35.5 41.0 30.0 41.0 38-39 36.25734867021424 39.0 35.0 40.5 30.0 41.0 40-41 36.132291932722055 39.0 35.0 41.0 29.5 41.0 42-43 35.91186759421446 39.0 35.0 40.5 28.5 41.0 44-45 35.5801904163134 39.0 35.0 40.0 27.0 41.0 46-47 35.191748542457276 38.5 35.0 40.0 25.5 41.0 48-49 34.76978381567129 38.0 34.0 40.0 24.0 41.0 50-51 34.03511502912566 37.5 33.5 39.5 23.0 40.5 52-53 34.09128099025752 38.0 33.5 40.0 23.0 40.5 54-55 34.165004465952215 38.0 34.0 40.0 22.5 41.0 56-57 33.84314784896266 38.0 33.5 40.0 21.5 41.0 58-59 33.4309963783722 37.5 33.0 40.0 19.0 41.0 60-61 32.924269994393 37.0 32.5 40.0 16.0 41.0 62-63 32.38084169471426 36.0 31.5 39.0 11.0 41.0 64-65 31.8329751503638 35.5 31.0 39.0 7.5 40.0 66-67 31.252016983212755 35.0 30.5 38.5 4.5 40.0 68-69 30.571005113804958 35.0 29.5 38.0 2.0 40.0 70-71 29.979949310308868 34.5 29.0 37.0 2.0 39.5 72-73 29.286921435008935 34.0 28.0 36.5 2.0 39.0 74-75 28.611140749786273 34.0 26.5 36.0 2.0 39.0 76-77 27.86601387694526 33.5 26.0 35.5 2.0 38.0 78-79 27.092499618391564 33.0 25.0 35.0 2.0 37.0 80-81 26.369134753873894 32.0 23.0 35.0 2.0 36.5 82-83 25.626499041067113 32.0 20.5 35.0 2.0 36.0 84-85 24.896864463262062 31.0 18.0 34.5 2.0 35.5 86-87 24.15033609250088 31.0 10.0 34.0 2.0 35.0 88-89 23.24705179144381 30.5 4.5 34.0 2.0 35.0 90-91 22.337631277080973 29.5 2.0 34.0 2.0 35.0 92-93 21.386045171356045 29.0 2.0 34.0 2.0 35.0 94-95 20.285556460668637 28.0 2.0 34.0 2.0 35.0 96-97 19.002847579796466 25.5 2.0 33.0 2.0 35.0 98-99 17.41143082257651 21.5 2.0 33.0 2.0 34.5 100-101 13.458421808556995 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20676969210461493 1101 2 0.08828238505585517 1101 3 -0.023597189594262602 1101 4 -0.005832644360964423 1101 5 0.005584314305245641 1101 6 -0.02004012485117812 1101 7 -0.039852040683449275 1101 8 0.015566584213544843 1101 9 -0.07824741141367753 1101 10-11 0.060368838489964105 1101 12-13 0.11555589481604756 1101 14-15 0.2676340100392949 1101 16-17 0.23140696608356848 1101 18-19 0.23968449595201236 1101 20-21 0.23617434352605216 1101 22-23 0.21878115406024534 1101 24-25 0.26190644764114523 1101 26-27 0.3057725541282039 1101 28-29 0.23832867352448517 1101 30-31 0.05250302144061436 1101 32-33 0.12376039072162115 1101 34-35 0.0589941058487824 1101 36-37 0.07833848420999345 1101 38-39 -0.03332021178576383 1101 40-41 0.14486055090756622 1101 42-43 0.2641405525457827 1101 44-45 0.2410754783363913 1101 46-47 0.04712501443577821 1101 48-49 -0.010434303339827977 1101 50-51 0.17745198131636641 1101 52-53 0.0406353338754144 1101 54-55 -0.009663443480540934 1101 56-57 0.010916754585956312 1101 58-59 0.05569476003505969 1101 60-61 0.025774100334018613 1101 62-63 -0.13906814078455199 1101 64-65 -0.22972314254007742 1101 66-67 -0.08311588405689818 1101 68-69 -0.2311973575517321 1101 70-71 -0.03879262466675115 1101 72-73 0.06878023015968537 1101 74-75 0.07074533259450533 1101 76-77 -0.16572759804864035 1101 78-79 -0.35912005590592244 1101 80-81 -0.28372918529797886 1101 82-83 -0.3388252640398477 1101 84-85 -0.5237387831779117 1101 86-87 -0.3441938272371807 1101 88-89 -0.3765911310845631 1101 90-91 -0.47170614085332474 1101 92-93 -0.6818624257995118 1101 94-95 -0.7632628892444338 1101 96-97 -0.741708495788707 1101 98-99 -0.9034744142991542 1101 100-101 -1.3030989663872443 1102 1 0.331228124574352 1102 2 0.23240114962153768 1102 3 0.25643614597408515 1102 4 0.22675338165594638 1102 5 0.2544222764874533 1102 6 0.2426701611560773 1102 7 0.10111332269930529 1102 8 0.2542450544617836 1102 9 0.310204408124541 1102 10-11 0.25816213493880014 1102 12-13 0.25698146784309017 1102 14-15 0.3647616110365206 1102 16-17 0.36900778721407335 1102 18-19 0.31187289948171326 1102 20-21 0.40087081173027883 1102 22-23 0.5289770157630116 1102 24-25 0.4452713759412248 1102 26-27 0.44934780399258045 1102 28-29 0.2490738406459414 1102 30-31 0.4007686169261575 1102 32-33 0.17396540243512248 1102 34-35 0.22093862874446302 1102 36-37 0.28469408437551635 1102 38-39 0.027416624829996294 1102 40-41 0.007625120657266393 1102 42-43 0.2319140251001528 1102 44-45 0.19099999582741134 1102 46-47 0.36541602812286555 1102 48-49 0.22807772611358956 1102 50-51 0.15850990135841414 1102 52-53 0.1762876294038307 1102 54-55 0.08455491465128517 1102 56-57 0.3078149619017623 1102 58-59 0.16023414063825925 1102 60-61 0.20324587312658338 1102 62-63 0.1822110185252015 1102 64-65 0.03957196799489182 1102 66-67 0.2099587565969223 1102 68-69 0.1474930395424785 1102 70-71 -0.14774192439631406 1102 72-73 -0.10167038247112359 1102 74-75 -0.025485215166725084 1102 76-77 -0.21423924822700258 1102 78-79 -0.28833395416944896 1102 80-81 -0.05415399367941376 1102 82-83 -0.09288113667386355 1102 84-85 -0.1908824220918035 1102 86-87 -0.16876506017234405 1102 88-89 -0.3290710142678428 1102 90-91 -0.23721442699739015 1102 92-93 -0.27233849049570935 1102 94-95 -0.3345207143322604 1102 96-97 -0.8351012533960507 1102 98-99 -0.85141230936323 1102 100-101 -1.0533675066726893 1103 1 0.15124618005530976 1103 2 0.1676239148721521 1103 3 0.12266127173587904 1103 4 0.08498205202066345 1103 5 0.09824556294303477 1103 6 0.05719868549133622 1103 7 0.04649421406720222 1103 8 0.1323449836208752 1103 9 0.12370740160052662 1103 10-11 0.21037297231927 1103 12-13 0.1217773079216542 1103 14-15 0.16702148720559507 1103 16-17 0.22739405942218127 1103 18-19 0.09165581173360948 1103 20-21 0.053890373539609016 1103 22-23 0.16925576323435365 1103 24-25 0.20448804682765598 1103 26-27 0.1458306295474756 1103 28-29 0.1058246340897071 1103 30-31 0.047257452449933623 1103 32-33 0.11094128839404505 1103 34-35 0.02104067034368029 1103 36-37 -0.05470132554272311 1103 38-39 -0.20691142228815806 1103 40-41 -0.047473506768142215 1103 42-43 0.11257842076581426 1103 44-45 0.08764547415700008 1103 46-47 0.08781556573888594 1103 48-49 0.1687833092468054 1103 50-51 0.21150887582755473 1103 52-53 0.20709926930696554 1103 54-55 0.18908553608206802 1103 56-57 0.24965988168786168 1103 58-59 0.28019407844147537 1103 60-61 0.24370095564218275 1103 62-63 0.11307591698229658 1103 64-65 -0.06227750402294774 1103 66-67 -0.2185437126051859 1103 68-69 -0.07307090013278028 1103 70-71 -0.3296646413114388 1103 72-73 -0.3903016951761984 1103 74-75 -0.17765858614547625 1103 76-77 -0.3933228942065874 1103 78-79 -0.5416525438982838 1103 80-81 -0.3684884727429818 1103 82-83 -0.6968257861392324 1103 84-85 -0.8443799042984637 1103 86-87 -0.9028709302496054 1103 88-89 -0.6489448944316223 1103 90-91 -0.897302095819974 1103 92-93 -1.1142158896880723 1103 94-95 -1.1150468807137734 1103 96-97 -1.3607762879853418 1103 98-99 -1.4769340258103405 1103 100-101 -1.2550611031147874 1104 1 0.28796616738505776 1104 2 0.23139753962139054 1104 3 0.21990549093131762 1104 4 0.15264914102921523 1104 5 0.1939191982508106 1104 6 0.1262251874446676 1104 7 0.17867402612505856 1104 8 0.20902643225354467 1104 9 0.3517456235411913 1104 10-11 0.32559311802722135 1104 12-13 0.3173282001188866 1104 14-15 0.2894412422472996 1104 16-17 0.28960369937572494 1104 18-19 0.316975207784715 1104 20-21 0.3324446444719271 1104 22-23 0.39058930773862244 1104 24-25 0.42002758858671285 1104 26-27 0.4537376615398756 1104 28-29 0.33320294683387175 1104 30-31 0.2708544854981838 1104 32-33 0.2544517413557159 1104 34-35 0.33811384107538345 1104 36-37 0.3856626803700678 1104 38-39 0.21220350749749883 1104 40-41 0.27250326442814554 1104 42-43 0.3489974895019543 1104 44-45 0.17875913691699452 1104 46-47 0.1724027265455561 1104 48-49 0.2993265464973547 1104 50-51 0.23064624252589283 1104 52-53 0.3292220652102458 1104 54-55 0.08197567439903253 1104 56-57 0.338945595973577 1104 58-59 0.2628120702058041 1104 60-61 0.3719675061648289 1104 62-63 0.596604583437788 1104 64-65 0.28186514194896617 1104 66-67 0.22251979705588454 1104 68-69 0.18180582882931517 1104 70-71 0.17867661093225706 1104 72-73 0.2003104365944708 1104 74-75 0.001328585219738443 1104 76-77 0.0094262875060096 1104 78-79 -0.25394533241058426 1104 80-81 -0.27805451645537005 1104 82-83 -0.3908390899086349 1104 84-85 -0.3182145046574547 1104 86-87 -0.22473504174786285 1104 88-89 -0.01212592072240426 1104 90-91 -0.326592825415581 1104 92-93 -0.5470767280367141 1104 94-95 -0.8165699511392823 1104 96-97 -0.7429956945949918 1104 98-99 -0.7743814970382985 1104 100-101 -1.1049251618203098 1105 1 0.16036468936516712 1105 2 0.24303513494099604 1105 3 0.31893825732809944 1105 4 0.2458472609009732 1105 5 0.23397833715932137 1105 6 0.26241675041447365 1105 7 0.3045887320606866 1105 8 0.2807425473797025 1105 9 0.3900727078933812 1105 10-11 0.26702590072569166 1105 12-13 0.31461238385704604 1105 14-15 0.3579570186605636 1105 16-17 0.3293345403617991 1105 18-19 0.3593448848301293 1105 20-21 0.3429614986080267 1105 22-23 0.4437497978761016 1105 24-25 0.4499977927200902 1105 26-27 0.47425062138337637 1105 28-29 0.41947850878934645 1105 30-31 0.37252835207790724 1105 32-33 0.3722857901521124 1105 34-35 0.2760893070320236 1105 36-37 0.3177265195991765 1105 38-39 0.3938948983939099 1105 40-41 0.2878042117374662 1105 42-43 0.1951604792318946 1105 44-45 0.15424433860512465 1105 46-47 0.3024913443433803 1105 48-49 0.3326567100576483 1105 50-51 0.14220391428231238 1105 52-53 0.23750241805126393 1105 54-55 0.26423988856701186 1105 56-57 0.3456564583329751 1105 58-59 0.2631822544149074 1105 60-61 0.19639151312021852 1105 62-63 0.19781090849348715 1105 64-65 0.04878380557265416 1105 66-67 -0.019451620647753742 1105 68-69 0.16850523472328405 1105 70-71 0.08152759537319554 1105 72-73 0.09993617696289192 1105 74-75 -0.08224474521551173 1105 76-77 0.04786544597654796 1105 78-79 -0.09697590713571813 1105 80-81 -0.27000555704657714 1105 82-83 -0.27736913935709495 1105 84-85 -0.1154407268313733 1105 86-87 -0.07913717726924574 1105 88-89 0.059248311739693804 1105 90-91 -0.10566983278756936 1105 92-93 -0.45350955601766074 1105 94-95 -0.7156647545227877 1105 96-97 -0.5618612781049244 1105 98-99 -0.36750263670755245 1105 100-101 -0.6105345280468306 1106 1 0.11120481775136426 1106 2 0.13883924493543276 1106 3 0.16790702935838908 1106 4 0.2397840446534829 1106 5 0.2092508811356879 1106 6 0.13018389885019133 1106 7 0.12898102696397729 1106 8 0.16150260281482076 1106 9 0.3212353536692163 1106 10-11 0.3305472639388185 1106 12-13 0.40343639659942454 1106 14-15 0.44047330152402964 1106 16-17 -0.061102751064765926 1106 18-19 0.31555844838923264 1106 20-21 0.45517325523245944 1106 22-23 0.4947029198309636 1106 24-25 0.2209276182101405 1106 26-27 0.07569114652527276 1106 28-29 0.07748830016178232 1106 30-31 0.19460603823909395 1106 32-33 0.23125631257518364 1106 34-35 0.2258628086115877 1106 36-37 0.06419505704074169 1106 38-39 0.1253905097802459 1106 40-41 0.1350424407971076 1106 42-43 0.049367346786702626 1106 44-45 0.22521705172597706 1106 46-47 0.11092694432509376 1106 48-49 0.0816073766505312 1106 50-51 0.141160445593286 1106 52-53 0.2507386356629837 1106 54-55 0.050075872552426404 1106 56-57 -0.28363380852072595 1106 58-59 -0.0963621081173116 1106 60-61 0.20069847186388046 1106 62-63 -0.09273023355660115 1106 64-65 -0.3486740045476253 1106 66-67 -0.49915232754177197 1106 68-69 -0.4895889183633031 1106 70-71 -0.7126884692626128 1106 72-73 -0.25482453050857856 1106 74-75 -0.44399424175202284 1106 76-77 -0.8701608327725978 1106 78-79 -0.5991031456535048 1106 80-81 -0.6353402008687112 1106 82-83 -0.5366713341451366 1106 84-85 -0.332421461683559 1106 86-87 -0.2880999402191442 1106 88-89 -0.6633169881368737 1106 90-91 -0.7934166020797804 1106 92-93 -0.8898217250370308 1106 94-95 -1.0754871648437323 1106 96-97 -1.044820211845039 1106 98-99 -1.2936796705917892 1106 100-101 -1.4606563938813295 1107 1 -0.07707855610337688 1107 2 -0.004834219789181304 1107 3 -0.012420130467155843 1107 4 0.04282932508357362 1107 5 0.11580718884741259 1107 6 0.026665255103281993 1107 7 0.08082347711557247 1107 8 0.06398328555476951 1107 9 0.06039073119291771 1107 10-11 0.0021111364326600324 1107 12-13 -0.08380888024004207 1107 14-15 -0.050631075060117325 1107 16-17 0.005890861441031348 1107 18-19 -0.04475109019985979 1107 20-21 -0.022105468770234893 1107 22-23 0.14236319610191828 1107 24-25 0.08000723366568963 1107 26-27 -0.0038019015460406536 1107 28-29 0.025596370187564332 1107 30-31 -0.05205590984598274 1107 32-33 0.07760582237403213 1107 34-35 -0.10365961725436534 1107 36-37 -0.11409184214935664 1107 38-39 -0.07399277414580752 1107 40-41 -0.045599714270863956 1107 42-43 0.06181033890420906 1107 44-45 0.045902065732107644 1107 46-47 0.22502091409752012 1107 48-49 0.13318308730754325 1107 50-51 -0.22298180712864735 1107 52-53 -0.1819229707692287 1107 54-55 -0.11193899261166251 1107 56-57 -0.06552245253421063 1107 58-59 -0.18687400530808418 1107 60-61 -0.1719550447941387 1107 62-63 -0.08071974786005143 1107 64-65 -0.2700972444505041 1107 66-67 -0.3529349634154322 1107 68-69 -0.5669345312521052 1107 70-71 -0.5378058580336571 1107 72-73 -0.28826679742978456 1107 74-75 -0.4792822774275436 1107 76-77 -0.5622620958545603 1107 78-79 -0.5489770115482244 1107 80-81 -0.5472447632946214 1107 82-83 -0.6428279950432341 1107 84-85 -0.7527866929548068 1107 86-87 -0.9032849686421613 1107 88-89 -0.9309087107800273 1107 90-91 -0.7839997948258599 1107 92-93 -1.1441711347337709 1107 94-95 -1.1358877745961813 1107 96-97 -1.1297069414055763 1107 98-99 -1.3416315758777806 1107 100-101 -1.1157745497392337 1108 1 0.06655111776457545 1108 2 0.007621237932127656 1108 3 -0.05022832938968236 1108 4 0.08834534702256747 1108 5 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-0.6568603566669111 2316 58-59 -0.5195127193132265 2316 60-61 -0.576465306026968 2316 62-63 -0.3658176865384348 2316 64-65 -0.3693956675852199 2316 66-67 -0.4709546441703587 2316 68-69 -0.46774141649131096 2316 70-71 -0.45701353824710367 2316 72-73 -0.27255996838025 2316 74-75 -0.1805265799987339 2316 76-77 -0.29768321969342537 2316 78-79 -0.19404260524167327 2316 80-81 -0.20794307314248783 2316 82-83 -0.3240470689799224 2316 84-85 -0.3119253801129318 2316 86-87 -0.2978168360526787 2316 88-89 -0.3766232838708454 2316 90-91 -0.17557792305175823 2316 92-93 -0.244638159411096 2316 94-95 -0.5155938489041887 2316 96-97 -0.532269470379557 2316 98-99 -0.41132908182760985 2316 100-101 -0.29569267767775287 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6167.0 3 1899.0 4 1667.0 5 1949.0 6 2270.0 7 2870.0 8 3605.0 9 4621.0 10 5896.0 11 7462.0 12 9421.0 13 11564.0 14 13859.0 15 16859.0 16 19813.0 17 24766.0 18 24189.0 19 21232.0 20 21549.0 21 22985.0 22 25249.0 23 27885.0 24 31606.0 25 35068.0 26 39655.0 27 45090.0 28 52235.0 29 60441.0 30 71071.0 31 83917.0 32 102818.0 33 127980.0 34 163477.0 35 212844.0 36 265299.0 37 273496.0 38 134271.0 39 7974.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.64188023019809 18.655590305122953 14.62364267735691 23.078886787322052 2 15.556683488208456 19.712605672280343 40.15103096628769 24.579679873223505 3 19.31122935509489 23.802931029089297 29.803286231953525 27.082553383862283 4 12.84532504741536 16.54422743230701 36.92632460352689 33.68412291675074 5 13.727346879441788 37.22657764246027 34.14862021902055 14.8974552590774 6 32.328451686434526 36.03264144981581 17.09567415486467 14.543232708884988 7 28.551049922140155 32.99970834094439 20.19582533962133 18.25341639729413 8 26.813223487136156 34.31137585689209 19.4698889203689 19.40551173560285 9 26.359508273535333 16.731268854213585 20.216205910583174 36.693016961667915 10-11 25.09706150363511 26.461394004400358 26.89880742563997 21.542737066324563 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25.989526097905497 29.1488397807605 22.31639977703945 38-39 21.975944908927097 26.428518324838237 29.00897813116262 22.586558635072045 40-41 22.486833562519408 26.258549111013433 29.136867436355494 22.117749890111664 42-43 22.19287869802128 26.269262148710503 28.95373253240467 22.584126620863547 44-45 22.157780573161094 26.54506245262632 29.091196356862937 22.205960617349653 46-47 22.63979669600771 26.76021056196901 28.269264887924745 22.33072785409854 48-49 22.337570507573894 26.82654431242264 28.258089975526463 22.577795204477006 50-51 22.400535494262364 27.14583927157861 28.14225477661739 22.311370457541642 52-53 22.776938905550153 26.744156456375272 28.15286550382431 22.326039134250266 54-55 22.331072354270177 27.024290299127152 28.289648932912808 22.354988413689863 56-57 22.388709146279854 27.111573926283217 28.093950360133306 22.40576656730362 58-59 22.414374046272854 26.721547586495415 28.042066691280336 22.822011675951394 60-61 22.09059161454177 26.93757082312695 28.39249865125804 22.57933891107324 62-63 22.175084468675838 26.86624299811569 28.514286275916138 22.444386257292333 64-65 21.948011303073876 27.028750064886335 28.35165017923748 22.671588452802308 66-67 21.971252980674468 27.007915623131368 28.200916169184126 22.819915227010032 68-69 22.49870949268948 26.586769477082406 28.34236352381087 22.57215750641725 70-71 22.19006431581282 26.79386177140668 27.980579245549304 23.035494667231195 72-73 22.298294805889324 26.87078155864147 27.950017061517734 22.880906573951474 74-75 22.189428961304543 27.30154890198435 27.819327948149013 22.68969418856209 76-77 22.369810385238363 26.82438797395817 27.94747393209584 22.858327708707627 78-79 22.51220798957553 27.1541413944948 27.732858803533876 22.60079181239579 80-81 22.650102392930858 27.127223216589268 27.475671600877195 22.747002789602682 82-83 22.612162137989465 27.226658759085932 27.6076435804108 22.553535522513805 84-85 22.56727229302853 27.3132727631391 27.487284214589096 22.63217072924327 86-87 22.752298293387405 27.162466818105774 27.30353395991768 22.78170092858914 88-89 22.615426222769 27.287774565840223 27.118423204992776 22.978376006397998 90-91 22.606131760275034 27.330400239246877 27.18011446934246 22.88335353113563 92-93 22.842557825598675 26.831531541751275 27.36335941172986 22.962551220920194 94-95 22.581733145756573 26.709018481318914 27.493447963203227 23.21580040972129 96-97 22.90494710601734 26.302235485976855 27.524228897229147 23.26858851077666 98-99 22.66600231966986 26.566243410086077 27.097746210374197 23.67000805986986 100-101 23.532942755327824 25.444570635323576 27.29006810524269 23.73241850410591 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 624.0 1 455.5 2 364.0 3 475.5 4 439.5 5 438.0 6 779.5 7 1658.5 8 2769.5 9 3162.5 10 2591.5 11 2011.0 12 1909.5 13 2019.5 14 2221.5 15 2372.5 16 2493.5 17 2699.5 18 3024.0 19 3339.5 20 3769.5 21 4480.5 22 5457.5 23 6720.0 24 8182.0 25 10071.0 26 13040.0 27 16732.0 28 19249.0 29 21293.0 30 23885.0 31 27776.0 32 33375.0 33 36139.5 34 36819.0 35 39165.0 36 43383.0 37 47532.0 38 50252.5 39 52340.5 40 55440.0 41 58970.5 42 60112.0 43 61407.5 44 63990.0 45 67346.5 46 69298.0 47 70465.0 48 71432.5 49 70180.5 50 68852.0 51 68016.5 52 67230.5 53 67768.0 54 68435.0 55 65516.0 56 60710.0 57 56053.0 58 52685.0 59 47484.5 60 39054.5 61 32310.0 62 27176.5 63 20830.0 64 14919.5 65 11437.5 66 8674.0 67 6655.0 68 5075.5 69 3618.5 70 2642.5 71 1821.5 72 1219.5 73 883.5 74 648.0 75 450.5 76 330.0 77 245.0 78 156.5 79 91.5 80 56.5 81 34.0 82 21.0 83 16.0 84 9.5 85 6.5 86 7.5 87 7.5 88 7.0 89 4.5 90 2.0 91 2.0 92 2.0 93 3.0 94 2.5 95 1.5 96 1.5 97 1.5 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14609431950021637 2 0.05314810588714768 3 0.1538020542876416 4 0.127908095590017 5 0.1931971432011482 6 0.19430544493528776 7 0.16387752459800134 8 0.07037716011786285 9 0.11949507788086663 10-11 0.10065394840049391 12-13 0.14984743219082539 14-15 0.1360692265414084 16-17 0.1325428119327825 18-19 0.13057809522226235 20-21 0.1322657364992476 22-23 0.1934742186346831 24-25 0.21128261240824395 26-27 0.14594318744556098 28-29 0.1255403600670825 30-31 0.09775725068626546 32-33 0.12274441705595765 34-35 0.09276989288263739 36-37 0.08606970512624816 38-39 0.051964238125680406 40-41 0.05979791629198511 42-43 0.06239234989690275 44-45 0.040049994483680004 46-47 0.05012546479403975 48-49 0.04206508854575195 50-51 0.05327404926602718 52-53 0.02732971321685082 54-55 0.050201030821367454 56-57 0.02748084527150622 58-59 0.05171235136792142 60-61 0.038412730558246545 62-63 0.05035216287602285 64-65 0.03929433421040303 66-67 0.03921876818307533 68-69 0.06564168907199378 70-71 0.05334961529335487 72-73 0.05148565328593832 74-75 0.043903861877392605 76-77 0.022291978061670947 78-79 0.023072827010723825 80-81 0.025541317236761965 82-83 0.021435563085290366 84-85 0.019118204913907623 86-87 0.02533980783055477 88-89 0.06415555720121571 90-91 0.10730375880533133 92-93 0.07941989472141073 94-95 0.07433178221467905 96-97 0.06720338697009953 98-99 0.0562211243318074 100-101 0.1295957368670023 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1985019.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.58920428665004 #Duplication Level Percentage of deduplicated Percentage of total 1 76.76990541484035 42.67577955172362 2 13.224243165142896 14.702503096869279 3 4.386173457913052 7.314716771658326 4 1.9032098961975084 4.2319169488038915 5 1.031819921044693 2.867902418899428 6 0.5910082729626709 1.9712207772493286 7 0.3969220897106735 1.5445208191567463 8 0.2694216646379176 1.1981548763845213 9 0.20312191726559534 1.0162247178575885 >10 1.1018583136136657 11.207026266296253 >50 0.07585284808479635 2.885594652736176 >100 0.04047528948213387 4.071606781208989 >500 0.0034474919084188506 1.3319211572169958 >1k 0.0023588102531286862 2.1681921390031453 >5k 1.8144694254836047E-4 0.8127190249357017 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9008 0.45379918277860315 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7107 0.3580318374786337 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4538 0.22861242134206272 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2557 0.1288148879179494 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2504 0.12614488828570405 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2294 0.11556564445982632 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2266 0.11415507861637596 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2187 0.11017526784378386 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 5.037735155179875E-5 0.0 8 0.0 0.0 0.0 5.037735155179875E-5 0.0 9 0.0 0.0 0.0 1.007547031035975E-4 0.0 10-11 0.0 0.0 0.0 1.007547031035975E-4 0.0 12-13 0.0 0.0 0.0 1.5113205465539623E-4 1.7632073043129563E-4 14-15 0.0 0.0 0.0 2.01509406207195E-4 2.2669808198309436E-4 16-17 0.0 0.0 0.0 2.770754335348931E-4 2.5188675775899373E-4 18-19 0.0 0.0 0.0 3.2745278508669183E-4 3.5264146086259125E-4 20-21 0.0 0.0 0.0 4.2820748819028936E-4 4.2820748819028936E-4 22-23 0.0 0.0 0.0 5.037735155179875E-4 5.037735155179875E-4 24-25 0.0 0.0 0.0 9.823583552600755E-4 5.037735155179875E-4 26-27 0.0 0.0 0.0 0.001410565843450365 6.549055701733838E-4 28-29 0.0 0.0 0.0 0.003702735339057208 7.052829217251825E-4 30-31 0.0 0.0 0.0 0.012266885102862994 7.052829217251825E-4 32-33 0.0 0.0 0.0 0.03100725988013213 7.304715975010819E-4 34-35 0.0 0.0 0.0 0.05992385967086461 8.564149763805787E-4 36-37 0.0 0.0 0.0 0.10700149469602054 8.564149763805787E-4 38-39 0.0 0.0 0.0 0.17531318340025964 9.067923279323774E-4 40-41 0.0 0.0 0.0 0.25062732397019877 9.067923279323774E-4 42-43 0.0 0.0 0.0 0.32521099294263683 9.067923279323774E-4 44-45 0.0 0.0 0.0 0.416797018063807 9.319810037082769E-4 46-47 0.0 0.0 0.0 0.5210025697487026 9.571696794841762E-4 48-49 0.0 0.0 0.0 0.630548120698089 0.0011083017341395724 50-51 0.0 0.0 0.0 0.7448795200448963 0.0012594337887949687 52-53 0.0 0.0 0.0 0.8569691272476485 0.0013601884918985662 54-55 0.0 0.0 0.0 0.9750536392850647 0.0013601884918985662 56-57 0.0 0.0 0.0 1.1102412621743167 0.0013601884918985662 58-59 0.0 2.5188675775899374E-5 0.0 1.257620204139104 0.0013601884918985662 60-61 0.0 5.037735155179875E-5 0.0 1.4186765970502044 0.0013601884918985662 62-63 0.0 5.037735155179875E-5 0.0 1.572529028689398 0.0014609431950021636 64-65 0.0 5.037735155179875E-5 0.0 1.7291270259881644 0.0015113205465539625 66-67 0.0 5.037735155179875E-5 0.0 1.9072865297511008 0.0015113205465539625 68-69 0.0 5.037735155179875E-5 0.0 2.105420653404325 0.0015113205465539625 70-71 0.0 5.037735155179875E-5 0.0 2.3056958144985007 0.0015113205465539625 72-73 0.0 5.037735155179875E-5 0.0 2.513729087731654 0.0015868865738816605 74-75 0.0 5.037735155179875E-5 0.0 2.7215860402343752 0.00161207524965756 76-77 0.0 5.037735155179875E-5 0.0 2.9455889339094488 0.00161207524965756 78-79 0.0 5.037735155179875E-5 0.0 3.192815786649901 0.0017128299527611574 80-81 0.0 5.037735155179875E-5 0.0 3.452687354629855 0.0017128299527611574 82-83 0.0 5.037735155179875E-5 0.0 3.725984486798363 0.0017632073043129563 84-85 0.0 5.037735155179875E-5 0.0 3.9945461479210023 0.0017632073043129563 86-87 0.0 5.037735155179875E-5 0.0 4.276231109122885 0.001813584655864755 88-89 0.0 5.037735155179875E-5 0.0 4.583407010209978 0.0018639620074165537 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4300 0.0 55.25096 1 GTATCAA 7535 0.0 41.13443 1 TATCAAC 9640 0.0 32.066406 2 ATCAACG 9635 0.0 31.935776 3 TCAACGC 9680 0.0 31.836443 4 CAACGCA 9990 0.0 30.896135 5 AACGCAG 10290 0.0 30.036282 6 TGGTATC 1815 0.0 28.56037 2 ACGCAGA 11735 0.0 26.048836 7 CGCAGAG 11810 0.0 25.800299 8 GTGGTAT 2045 0.0 25.377039 1 GCAGAGT 12990 0.0 23.090685 9 GAGTACT 7970 0.0 22.992311 12-13 TACATGG 6940 0.0 22.065325 2 GTACTTT 8370 0.0 22.034107 14-15 GTACATG 7195 0.0 21.439861 1 CAGAGTA 12410 0.0 20.79858 10-11 AGAGTAC 11500 0.0 20.791018 10-11 GTGTAGC 1325 0.0 20.481735 1 GGTACGC 140 4.8193082E-4 20.37846 6 >>END_MODULE