FastQCFastQC Report
Thu 26 May 2016
SRR938148_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938148_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1985019
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88470.4456884291787635No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73850.3720367412100337No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48260.2431210985889808No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA24680.12433130362983931No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA24310.12246734162242275No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA24240.12211470016156016No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC23890.12035149285724721No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA22810.11491073888965295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43450.053.144721
GTATCAA76900.040.9020731
TATCAAC96850.032.3270872
ATCAACG99550.031.3469643
TCAACGC100050.031.235424
CAACGCA103050.030.372185
AACGCAG104850.029.8047246
TGGTATC19350.028.4811692
ACGCAGA117900.026.3043167
CGCAGAG118500.025.9707378
GTGGTAT22150.025.526241
GCAGAGT131700.023.2235039
GAGTACT80900.022.57398812-13
TAAGGTG12950.022.0049345
GTACATG73350.021.8942571
TACATGG71050.021.7988872
GTACTTT85550.021.23542414-15
CAGAGTA124150.021.1751210-11
GTGTAAG9000.021.1169471
AGTACTT82100.020.94237112-13
Produced by FastQC (version 0.11.5)