FastQCFastQC Report
Thu 26 May 2016
SRR938147_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938147_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2118165
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118250.5582662351610946No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100700.47541150004839094No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61200.2889293327007103No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26750.126288556368366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66100.046.7737541
GTATCAA107250.037.235471
TATCAAC132650.030.1300852
ATCAACG133800.029.8000833
TCAACGC135500.029.5314274
CAACGCA136650.029.1785665
AACGCAG140650.028.3440596
ACGCAGA156850.025.1985767
CGCAGAG157950.024.9328638
TGGTATC33650.024.2915382
GAGTACT95200.024.0999812-13
GCAGAGT167100.023.1411639
GTGGTAT36050.023.0288721
GTACTTT100950.022.44600314-15
TACATGG88250.022.2944762
GTACATG91550.021.8886831
CAGAGTA158050.021.30951510-11
TAAGGTG11150.020.8849225
AGTACTT98150.020.56861712-13
AGAGTAC142800.020.45826310-11
Produced by FastQC (version 0.11.5)