##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938147_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2118165 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.729263301017625 33.0 31.0 34.0 30.0 34.0 2 31.898216144634624 33.0 31.0 34.0 30.0 34.0 3 31.97111225990421 34.0 31.0 34.0 30.0 34.0 4 35.515143532255514 37.0 35.0 37.0 33.0 37.0 5 35.5221750902314 37.0 35.0 37.0 33.0 37.0 6 35.5514886706182 37.0 35.0 37.0 33.0 37.0 7 35.47634343877838 37.0 35.0 37.0 33.0 37.0 8 35.443492362493004 37.0 35.0 37.0 33.0 37.0 9 37.04453807895041 39.0 37.0 39.0 33.0 39.0 10-11 36.925212389025404 39.0 37.0 39.0 33.0 39.0 12-13 36.77105914789452 39.0 37.0 39.0 33.0 39.0 14-15 37.86348395899281 40.0 38.0 41.0 33.0 41.0 16-17 37.65867201091511 40.0 37.5 41.0 32.0 41.0 18-19 37.86751740303518 40.0 38.0 41.0 33.0 41.0 20-21 37.8498280351153 40.0 38.0 41.0 32.5 41.0 22-23 37.72366057412902 40.0 38.0 41.0 32.0 41.0 24-25 37.56112602181605 40.0 37.5 41.0 32.0 41.0 26-27 37.325731234346705 40.0 37.0 41.0 31.5 41.0 28-29 37.08268902564248 40.0 37.0 41.0 31.0 41.0 30-31 36.785033035670025 39.0 36.0 41.0 30.0 41.0 32-33 36.41445331218296 39.0 35.5 41.0 30.0 41.0 34-35 36.074850873279466 39.0 35.0 40.0 29.5 41.0 36-37 35.717908897559916 38.5 35.0 40.0 27.5 41.0 38-39 35.34138936296276 38.0 35.0 40.0 26.5 41.0 40-41 35.11949092728848 38.0 34.5 40.0 25.5 41.0 42-43 34.7942582376727 38.0 34.0 40.0 24.5 41.0 44-45 34.352422497775194 38.0 33.5 40.0 23.5 41.0 46-47 33.798712092778416 38.0 33.0 40.0 20.5 41.0 48-49 33.23103535371418 38.0 32.5 40.0 16.0 41.0 50-51 32.36329346391806 37.0 31.0 39.5 12.5 40.5 52-53 32.29819608009763 37.0 31.0 39.5 8.5 40.5 54-55 32.20593509004256 37.0 31.0 40.0 7.0 41.0 56-57 31.660904367695622 36.5 30.5 40.0 2.0 41.0 58-59 31.11805123774588 36.0 29.5 39.0 2.0 41.0 60-61 30.471295201270912 35.0 28.5 39.0 2.0 40.5 62-63 29.77402279803509 35.0 28.0 39.0 2.0 40.0 64-65 29.10118616821636 34.5 26.5 38.0 2.0 40.0 66-67 28.33650565465863 34.0 25.5 38.0 2.0 40.0 68-69 27.55839936926538 34.0 23.0 37.0 2.0 39.5 70-71 26.654607171773684 33.0 21.0 36.0 2.0 39.0 72-73 25.7929415319392 32.5 15.5 36.0 2.0 39.0 74-75 24.89886198667243 31.5 7.5 35.0 2.0 38.0 76-77 24.01546668932779 31.0 2.0 35.0 2.0 37.0 78-79 23.13163587350372 30.5 2.0 35.0 2.0 36.5 80-81 22.292039808041395 30.0 2.0 34.0 2.0 36.0 82-83 21.436487714602027 29.0 2.0 34.0 2.0 35.5 84-85 20.598081358156705 28.5 2.0 34.0 2.0 35.0 86-87 19.80392533159598 26.5 2.0 34.0 2.0 35.0 88-89 18.953605125190908 25.5 2.0 34.0 2.0 35.0 90-91 18.08607615554029 23.5 2.0 33.0 2.0 35.0 92-93 17.184353674052776 19.0 2.0 32.5 2.0 35.0 94-95 16.250009088054988 7.0 2.0 32.0 2.0 34.5 96-97 15.175211562838589 2.0 2.0 32.0 2.0 34.0 98-99 13.931742333576468 2.0 2.0 31.0 2.0 34.0 100-101 11.22508586441566 2.0 2.0 25.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5110912112036168 1101 2 0.46222583287737606 1101 3 0.4595581945520628 1101 4 0.3865853525477334 1101 5 0.3459475651501194 1101 6 0.2315685195778272 1101 7 0.3918819355994785 1101 8 0.4771060838215604 1101 9 0.492667968554656 1101 10-11 0.43334716313929533 1101 12-13 0.6156858893079189 1101 14-15 0.8323794486809462 1101 16-17 0.8591211317186378 1101 18-19 0.8693852639586552 1101 20-21 0.7271854651809377 1101 22-23 0.8854296460222031 1101 24-25 1.0086982659226464 1101 26-27 1.1513962362738681 1101 28-29 1.1876811856293799 1101 30-31 1.0563069817272392 1101 32-33 1.2629753354245068 1101 34-35 1.3433090693239222 1101 36-37 1.3486767753956386 1101 38-39 1.5121699128620563 1101 40-41 1.5768374586987974 1101 42-43 1.6320578803216605 1101 44-45 1.8304751818757552 1101 46-47 1.9494247162400526 1101 48-49 2.1257170923540016 1101 50-51 2.358418603153005 1101 52-53 2.4333849803953953 1101 54-55 2.4730100785591134 1101 56-57 2.658891189157867 1101 58-59 3.027204891721478 1101 60-61 3.1617237445393833 1101 62-63 3.411210029239836 1101 64-65 3.352639382746002 1101 66-67 3.5040491370767732 1101 68-69 3.6715311859368924 1101 70-71 4.08606830233375 1101 72-73 4.357240155969759 1101 74-75 4.680477039715843 1101 76-77 4.98844729688296 1101 78-79 5.181032786975475 1101 80-81 5.321997289643004 1101 82-83 5.593948511403699 1101 84-85 5.71069609773452 1101 86-87 5.881470714229518 1101 88-89 5.911816495498101 1101 90-91 6.10312425322925 1101 92-93 6.339420895128381 1101 94-95 6.155771843810356 1101 96-97 6.06794473577982 1101 98-99 6.000432522960487 1101 100-101 4.762377525701158 1102 1 0.5955038248956761 1102 2 0.48287648734427435 1102 3 0.4148565624695699 1102 4 0.31270956509969494 1102 5 0.36871139428070876 1102 6 0.35384762565411165 1102 7 0.4491809207673185 1102 8 0.38717997231710655 1102 9 0.5406192420873239 1102 10-11 0.5484861352359545 1102 12-13 0.6308693397372735 1102 14-15 0.8390049048107926 1102 16-17 0.7919316177124927 1102 18-19 0.8069522083879761 1102 20-21 0.7507199495045924 1102 22-23 0.8892829219875651 1102 24-25 0.8181412882707448 1102 26-27 0.8646934423084005 1102 28-29 0.9718069594615955 1102 30-31 0.872363659831187 1102 32-33 0.9563653105529539 1102 34-35 1.2654565558442599 1102 36-37 1.438401372821339 1102 38-39 1.4333642922531595 1102 40-41 1.5537150797434052 1102 42-43 1.914060494558349 1102 44-45 1.8864271089675952 1102 46-47 1.9337656199671542 1102 48-49 2.066591772382317 1102 50-51 2.358995913756992 1102 52-53 2.451718222956629 1102 54-55 2.642102175939435 1102 56-57 2.805729596652011 1102 58-59 2.851715176201264 1102 60-61 3.1226572448934 1102 62-63 3.3606481498941143 1102 64-65 3.281951511715018 1102 66-67 3.6029998115135307 1102 68-69 4.079168751065879 1102 70-71 4.009042722753847 1102 72-73 4.418443249483186 1102 74-75 4.632952395088644 1102 76-77 5.051730887101357 1102 78-79 5.332348074813826 1102 80-81 5.5048241146162304 1102 82-83 5.6121238104977955 1102 84-85 5.9349667438503815 1102 86-87 6.022559616431646 1102 88-89 6.076603271817476 1102 90-91 6.349232852978719 1102 92-93 6.239529821657438 1102 94-95 5.935190176145198 1102 96-97 5.697789697020129 1102 98-99 5.605265229966278 1102 100-101 4.529804862629067 1103 1 0.5091288113878072 1103 2 0.3970511346063397 1103 3 0.24081912595655908 1103 4 0.1599123408573817 1103 5 0.2696237961113539 1103 6 0.2593188239554749 1103 7 0.3260017085754825 1103 8 0.3701510026827961 1103 9 0.3393365055827786 1103 10-11 0.3004365255768775 1103 12-13 0.40413617054280593 1103 14-15 0.6514289321711573 1103 16-17 0.7441604892139537 1103 18-19 0.6305109552081447 1103 20-21 0.6333391911362938 1103 22-23 0.7815602173761889 1103 24-25 0.8061271699914485 1103 26-27 0.8684192778248985 1103 28-29 0.9741091385365124 1103 30-31 1.0131803529294743 1103 32-33 1.0802908873024748 1103 34-35 0.9818923474638339 1103 36-37 1.1709140118063672 1103 38-39 1.3094390075656719 1103 40-41 1.4775610695854837 1103 42-43 1.6277979249380579 1103 44-45 1.6280763249270365 1103 46-47 2.0264523863437987 1103 48-49 2.123246114030657 1103 50-51 2.2815584369394273 1103 52-53 2.4962800276664154 1103 54-55 2.638396346673069 1103 56-57 2.886341495843876 1103 58-59 3.08052972556413 1103 60-61 3.2268413257595334 1103 62-63 3.334795109674257 1103 64-65 3.5041777254518642 1103 66-67 3.6599190534372887 1103 68-69 3.603107178545411 1103 70-71 3.8547260457189765 1103 72-73 4.108879385735971 1103 74-75 4.288149931253226 1103 76-77 4.519515421247789 1103 78-79 4.534630539700668 1103 80-81 4.85078650286599 1103 82-83 5.156981912152908 1103 84-85 5.165666553717987 1103 86-87 5.29850538635101 1103 88-89 5.377117739110908 1103 90-91 5.445067464083504 1103 92-93 5.460010526578394 1103 94-95 5.4941561888849435 1103 96-97 5.1301342542978805 1103 98-99 5.0591099347353605 1103 100-101 4.158989892696855 1104 1 0.6711323538028999 1104 2 0.54160261574253 1104 3 0.4444232666264405 1104 4 0.48423916531782396 1104 5 0.41874774841542006 1104 6 0.5150696926138849 1104 7 0.4735075172250589 1104 8 0.5155969769636144 1104 9 0.542490343958427 1104 10-11 0.7006528984682845 1104 12-13 0.6568699304919647 1104 14-15 0.9327281362389357 1104 16-17 1.0601583879884515 1104 18-19 0.95891675174596 1104 20-21 1.0484137340836313 1104 22-23 1.0561910028300687 1104 24-25 1.08254888628489 1104 26-27 1.1964499150484755 1104 28-29 1.1774620155264799 1104 30-31 1.33628530531022 1104 32-33 1.4464176264249318 1104 34-35 1.454308901253988 1104 36-37 1.5548689437971106 1104 38-39 1.756576407268554 1104 40-41 1.8369282740221635 1104 42-43 1.94535146396408 1104 44-45 1.96063498645416 1104 46-47 2.235191667704676 1104 48-49 2.3291231562251724 1104 50-51 2.534541793811073 1104 52-53 2.5406242475183802 1104 54-55 2.607706620232399 1104 56-57 2.870046025312707 1104 58-59 3.2115160237070306 1104 60-61 3.2100167986463894 1104 62-63 3.320679618941977 1104 64-65 3.5827515866954194 1104 66-67 3.80770057154216 1104 68-69 3.735444383571018 1104 70-71 4.148072294570305 1104 72-73 4.417210617103013 1104 74-75 4.537492117661152 1104 76-77 4.626234425949157 1104 78-79 4.929322445148852 1104 80-81 4.9403406841491915 1104 82-83 5.295866695880022 1104 84-85 5.528155455891554 1104 86-87 5.74318973583226 1104 88-89 5.5753442258862975 1104 90-91 5.547959038180316 1104 92-93 5.658623978866348 1104 94-95 5.792240890163125 1104 96-97 5.6696365438669805 1104 98-99 5.442297880523519 1104 100-101 4.386820074562312 1105 1 0.4049627449380715 1105 2 0.4628877741813433 1105 3 0.4098958599327851 1105 4 0.34602087533630765 1105 5 0.3559338688509115 1105 6 0.41422945109354004 1105 7 0.3507280433120883 1105 8 0.30567249134643504 1105 9 0.38747062747775374 1105 10-11 0.47852082161329434 1105 12-13 0.42074766212788006 1105 14-15 0.6635675398743075 1105 16-17 0.8441910497918883 1105 18-19 0.6965222074244579 1105 20-21 0.6251008940201572 1105 22-23 0.7095001247789909 1105 24-25 0.6945662514508726 1105 26-27 0.8804950142069288 1105 28-29 0.9221496939043377 1105 30-31 0.9195350683458727 1105 32-33 1.1216603512268506 1105 34-35 1.1345843035696888 1105 36-37 1.3026248521953292 1105 38-39 1.365846295253803 1105 40-41 1.4620453160787648 1105 42-43 1.5175552847195348 1105 44-45 1.7003360709915114 1105 46-47 1.7210252585130732 1105 48-49 1.7992334428342076 1105 50-51 2.012430140402195 1105 52-53 1.9055075720364698 1105 54-55 2.201232125974535 1105 56-57 2.65294780424723 1105 58-59 2.7262358297366553 1105 60-61 2.8879199391439947 1105 62-63 3.003788643486768 1105 64-65 3.0664045818723444 1105 66-67 3.2143823114103007 1105 68-69 3.3166789023370242 1105 70-71 3.5828510667546176 1105 72-73 3.8466355661753155 1105 74-75 3.990727924675298 1105 76-77 4.1334579108174765 1105 78-79 4.289919202534023 1105 80-81 4.4359578911643816 1105 82-83 4.523822204188175 1105 84-85 4.596324467763335 1105 86-87 4.6626733106684775 1105 88-89 4.837440011848585 1105 90-91 5.005680802766058 1105 92-93 5.1643932096355805 1105 94-95 4.8832080974401535 1105 96-97 4.931248974937846 1105 98-99 4.600580504360298 1105 100-101 3.711709447562516 1106 1 0.35191652228391845 1106 2 0.28716631996765685 1106 3 0.35486452254013656 1106 4 0.31326784572445376 1106 5 0.311020245018625 1106 6 0.25318500356383566 1106 7 0.19793742866284703 1106 8 0.2101350947300844 1106 9 0.3761507381503151 1106 10-11 0.21187357553542796 1106 12-13 0.46732162234128793 1106 14-15 0.646051449709887 1106 16-17 0.6305237071234515 1106 18-19 0.5621128081900437 1106 20-21 0.6797443568020469 1106 22-23 0.6386415446802189 1106 24-25 0.7033842317757504 1106 26-27 0.7704591150419304 1106 28-29 1.0002373196010055 1106 30-31 0.9559895795420488 1106 32-33 0.9595842985483003 1106 34-35 0.9637314256863547 1106 36-37 1.2308597438036557 1106 38-39 1.322959189202514 1106 40-41 1.497237034478566 1106 42-43 1.3760629901480428 1106 44-45 1.596317496644545 1106 46-47 1.5840767619145666 1106 48-49 1.7467585573743563 1106 50-51 1.867087486839047 1106 52-53 1.9673675603345373 1106 54-55 2.039264787272849 1106 56-57 1.962275141163861 1106 58-59 2.425067495378581 1106 60-61 2.8114300494198794 1106 62-63 2.603602949601534 1106 64-65 2.9009274056094263 1106 66-67 3.168460635058132 1106 68-69 3.0449880467072 1106 70-71 3.419478751754319 1106 72-73 3.714838897260819 1106 74-75 3.7951579262620037 1106 76-77 3.4913965103533684 1106 78-79 3.919118760240444 1106 80-81 4.209803192538864 1106 82-83 4.118512573890076 1106 84-85 4.288284963806493 1106 86-87 4.231034940212897 1106 88-89 4.325643491687533 1106 90-91 4.580765220346734 1106 92-93 4.491017225383612 1106 94-95 4.391291731586016 1106 96-97 4.309912400432205 1106 98-99 4.0948942799211 1106 100-101 3.021084013698296 1107 1 0.45941671622518854 1107 2 0.33318470560048397 1107 3 0.4305743476051056 1107 4 0.3352889077468717 1107 5 0.34043337872174106 1107 6 0.3160646436088328 1107 7 0.3390083723810875 1107 8 0.31868014728816974 1107 9 0.296284266717862 1107 10-11 0.37435224199521855 1107 12-13 0.4311210288809946 1107 14-15 0.638945683835793 1107 16-17 0.5864954013944299 1107 18-19 0.533002707728464 1107 20-21 0.6342309457629085 1107 22-23 0.601347849711054 1107 24-25 0.7061301977972434 1107 26-27 0.814425601989683 1107 28-29 0.7907361365592251 1107 30-31 0.9705506904376691 1107 32-33 1.0299379504624966 1107 34-35 1.024645228486861 1107 36-37 1.168789808580037 1107 38-39 1.099772540119858 1107 40-41 1.27941698289019 1107 42-43 1.3193836526514318 1107 44-45 1.450996814747029 1107 46-47 1.5537150129521606 1107 48-49 1.5901536448418767 1107 50-51 1.8521101294828597 1107 52-53 2.063252853767679 1107 54-55 1.9242874299166601 1107 56-57 2.183603832836031 1107 58-59 2.567255298411819 1107 60-61 2.930973754086107 1107 62-63 2.772492344892637 1107 64-65 2.9160143397857574 1107 66-67 3.1860249265906546 1107 68-69 3.3638708964802895 1107 70-71 3.702605133585898 1107 72-73 3.9116812908840295 1107 74-75 3.955737665217047 1107 76-77 4.135762758753351 1107 78-79 4.301458952630188 1107 80-81 4.359114943440691 1107 82-83 4.421584361252862 1107 84-85 4.510142387878485 1107 86-87 4.481617733743015 1107 88-89 4.807986350769802 1107 90-91 4.630235033387343 1107 92-93 4.6338513377144 1107 94-95 4.634818699120245 1107 96-97 4.801566380107159 1107 98-99 4.558992113597064 1107 100-101 3.6772341339418873 1108 1 0.5386604446405947 1108 2 0.4496284410281426 1108 3 0.40695181723328133 1108 4 0.3429270053223874 1108 5 0.2812955353404334 1108 6 0.2519060264253028 1108 7 0.3477368754401837 1108 8 0.356428863169171 1108 9 0.42409661880313365 1108 10-11 0.5137444548872452 1108 12-13 0.5080770094570468 1108 14-15 0.6439910005267109 1108 16-17 0.7701945406402615 1108 18-19 0.8112033633742115 1108 20-21 0.7654091797267597 1108 22-23 0.7264902861186755 1108 24-25 0.7253288724812492 1108 26-27 0.8536766932716944 1108 28-29 0.9890067394377553 1108 30-31 0.9408239930339519 1108 32-33 1.0450353331387277 1108 34-35 1.1635474601366695 1108 36-37 1.1962568071600614 1108 38-39 1.2807684554819474 1108 40-41 1.3973753651632066 1108 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2311 70-71 3.5770549543221932 2311 72-73 3.7687104686028654 2311 74-75 4.081870815886003 2311 76-77 4.096594367462945 2311 78-79 4.289512411108049 2311 80-81 4.26488490292418 2311 82-83 4.422880015201034 2311 84-85 4.750294543415254 2311 86-87 4.6969804876999355 2311 88-89 5.042231548542851 2311 90-91 5.436107012866749 2311 92-93 5.387174274724341 2311 94-95 5.5110815609268755 2311 96-97 5.270134019364454 2311 98-99 4.912389379461171 2311 100-101 4.3653560915598675 2312 1 -0.325389381607728 2312 2 -0.22972555003007855 2312 3 -0.18525812416606158 2312 4 -0.029468131722808266 2312 5 -0.1877549080872356 2312 6 -0.18528750218775514 2312 7 -0.2215807334187474 2312 8 -0.06309325172661318 2312 9 -0.14415442046411897 2312 10-11 -0.1070593009105778 2312 12-13 -0.0744669619560483 2312 14-15 0.026099285165642527 2312 16-17 0.03180546241330262 2312 18-19 -0.04771958544593957 2312 20-21 0.008366849592299275 2312 22-23 -0.11463666244203807 2312 24-25 -0.10743383147559626 2312 26-27 -0.0022329558566127616 2312 28-29 0.11821794239352101 2312 30-31 0.055885888799686256 2312 32-33 0.17984571263080085 2312 34-35 0.2699664391337464 2312 36-37 0.36744070359109315 2312 38-39 0.41337508628944875 2312 40-41 0.5594865160248546 2312 42-43 0.6401584504377311 2312 44-45 0.7566861658386728 2312 46-47 0.8187416706991186 2312 48-49 1.0143250247603817 2312 50-51 1.2361833926748957 2312 52-53 1.3553173594337196 2312 54-55 1.4620643968124014 2312 56-57 1.8745584437140117 2312 58-59 1.9439535894779247 2312 60-61 2.052141829660311 2312 62-63 2.192595124190813 2312 64-65 2.358265348320291 2312 66-67 2.3787705731677207 2312 68-69 2.816755609326691 2312 70-71 3.0352958429049615 2312 72-73 3.25005628328201 2312 74-75 2.9612496358631155 2312 76-77 2.9983375119411306 2312 78-79 3.448144472304211 2312 80-81 3.8322443399417345 2312 82-83 4.085010540579422 2312 84-85 4.628102411394064 2312 86-87 4.681474815784004 2312 88-89 4.722332881208288 2312 90-91 4.937835025278524 2312 92-93 4.774681275479203 2312 94-95 4.644753017675253 2312 96-97 4.419394635291736 2312 98-99 4.208638366308449 2312 100-101 3.8190411845612005 2313 1 -0.42784763580209173 2313 2 -0.2880816358167202 2313 3 -0.32594445686628504 2313 4 -0.2091838249801654 2313 5 -0.2211950790274031 2313 6 -0.3845534882593 2313 7 -0.15333326586571872 2313 8 -0.07847786711122495 2313 9 -0.1791724245793418 2313 10-11 -0.14247448372159255 2313 12-13 -0.2135551821206576 2313 14-15 -0.2445609316751316 2313 16-17 -0.05494469586463424 2313 18-19 -0.03376342843422009 2313 20-21 -0.1333581820632901 2313 22-23 -0.12163947187540458 2313 24-25 -0.1370565364455274 2313 26-27 -0.16041295743939088 2313 28-29 0.03316086341283864 2313 30-31 -0.10358605643614993 2313 32-33 0.16200581076311948 2313 34-35 0.2774475447051259 2313 36-37 0.2540795726952396 2313 38-39 0.40107787989502697 2313 40-41 0.7017768966832918 2313 42-43 0.5600376645877745 2313 44-45 0.8007359046800815 2313 46-47 1.0182391382521487 2313 48-49 1.2720588804109028 2313 50-51 1.2946661008103817 2313 52-53 1.4344597897914326 2313 54-55 1.68198763201341 2313 56-57 1.930840593919772 2313 58-59 1.9968753012538052 2313 60-61 2.0404752619173543 2313 62-63 2.0670846778470384 2313 64-65 2.3236667807356106 2313 66-67 2.40600921672581 2313 68-69 2.672966039842674 2313 70-71 3.2631483080838137 2313 72-73 3.550618664573559 2313 74-75 3.723189233045769 2313 76-77 3.8981559869332187 2313 78-79 3.9932625872139944 2313 80-81 4.072438527184534 2313 82-83 4.284702887841217 2313 84-85 4.305523569197167 2313 86-87 4.551358481501005 2313 88-89 4.719997292414366 2313 90-91 4.563215881562158 2313 92-93 4.585106350661224 2313 94-95 4.544953338188073 2313 96-97 4.511491620097056 2313 98-99 4.185633162921302 2313 100-101 3.641492415172152 2314 1 -0.3625119298413857 2314 2 -0.357937442888435 2314 3 -0.4046181505967965 2314 4 -0.3200857071888663 2314 5 -0.2906540291681736 2314 6 -0.3047932863400078 2314 7 -0.3387359313820255 2314 8 -0.22381737608709784 2314 9 -0.18295415421334837 2314 10-11 -0.29163521881982746 2314 12-13 -0.363652953667291 2314 14-15 -0.401041288439572 2314 16-17 -0.3045161471406317 2314 18-19 -0.3736224330659539 2314 20-21 -0.3904755911693343 2314 22-23 -0.3332285456803916 2314 24-25 -0.29451508168816787 2314 26-27 -0.22270341319575948 2314 28-29 -0.21290959075421512 2314 30-31 -0.18382920344065923 2314 32-33 -0.1662161566633955 2314 34-35 0.035965778365408596 2314 36-37 0.31566926141560714 2314 38-39 0.31854535560113106 2314 40-41 0.2356126158883285 2314 42-43 0.3679085342484214 2314 44-45 0.48937160609986563 2314 46-47 0.4495597116181713 2314 48-49 0.637974215076234 2314 50-51 0.8351421966842025 2314 52-53 0.9312799677195613 2314 54-55 1.2060601504041202 2314 56-57 1.3862623028926215 2314 58-59 1.7409094394640725 2314 60-61 1.9119960233528879 2314 62-63 1.9846841238555761 2314 64-65 2.141309838435486 2314 66-67 2.3230935528442345 2314 68-69 2.680548419584145 2314 70-71 2.877074864481962 2314 72-73 3.2312025220040077 2314 74-75 3.264318321760083 2314 76-77 3.552727977442707 2314 78-79 3.7200533154251794 2314 80-81 3.9604224655946076 2314 82-83 4.267669258591678 2314 84-85 4.478147110428662 2314 86-87 4.656028432761865 2314 88-89 4.686543530267421 2314 90-91 4.576500418994421 2314 92-93 4.858057121481313 2314 94-95 4.82239961350367 2314 96-97 4.720123423899311 2314 98-99 4.73177570409225 2314 100-101 4.114603347800131 2315 1 -0.6645894854780039 2315 2 -0.478632457878458 2315 3 -0.480411689085777 2315 4 -0.4246049127259468 2315 5 -0.34669638130343117 2315 6 -0.30878007688806264 2315 7 -0.31207929491042563 2315 8 -0.36459382887166925 2315 9 -0.48531380908620747 2315 10-11 -0.4526262093606448 2315 12-13 -0.3519848265351726 2315 14-15 -0.42256963941282066 2315 16-17 -0.47355090288706236 2315 18-19 -0.4982918219978174 2315 20-21 -0.3227775285081762 2315 22-23 -0.31000873495489856 2315 24-25 -0.3140521572957553 2315 26-27 -0.28395915000852767 2315 28-29 -0.14049821465960122 2315 30-31 -0.31141793334146683 2315 32-33 -0.16791798882449172 2315 34-35 0.01723351083115432 2315 36-37 0.03619182185911285 2315 38-39 0.14145956639180213 2315 40-41 0.22743279799149008 2315 42-43 0.5057252163828494 2315 44-45 0.517163604929678 2315 46-47 0.6098898964332946 2315 48-49 0.8194442776544193 2315 50-51 0.947712359973174 2315 52-53 1.13571469490779 2315 54-55 1.3628677288948623 2315 56-57 1.6274276225846442 2315 58-59 1.9904612681197555 2315 60-61 2.2029268736072503 2315 62-63 2.2653222889597977 2315 64-65 2.6081778661136177 2315 66-67 2.744802459442436 2315 68-69 2.9117927952022313 2315 70-71 3.159225432791544 2315 72-73 3.6696169880164966 2315 74-75 3.87569172105891 2315 76-77 4.082201517882005 2315 78-79 4.3590216377857125 2315 80-81 4.494466831339981 2315 82-83 4.688412925478964 2315 84-85 5.002864863555942 2315 86-87 4.981169380170119 2315 88-89 5.269092081090484 2315 90-91 5.197877665926953 2315 92-93 5.45004153591492 2315 94-95 5.584063008697441 2315 96-97 5.3530737806420365 2315 98-99 5.252984403166565 2315 100-101 4.805068257335314 2316 1 -0.7964539523982097 2316 2 -0.8239618905240604 2316 3 -0.7245737628070223 2316 4 -0.6762505244887578 2316 5 -0.536479344098197 2316 6 -0.6136724125039379 2316 7 -0.5763569728671314 2316 8 -0.5169831948006163 2316 9 -0.6681094106641012 2316 10-11 -0.7055857843832953 2316 12-13 -0.5691519167822605 2316 14-15 -0.8175923930189697 2316 16-17 -0.4835922777261672 2316 18-19 -0.765898031655162 2316 20-21 -0.7502345200674725 2316 22-23 -0.7153738111446089 2316 24-25 -0.6966745058417203 2316 26-27 -0.6293391786723035 2316 28-29 -0.654828496412982 2316 30-31 -0.6760996493422908 2316 32-33 -0.42015038297988383 2316 34-35 -0.34698524795287966 2316 36-37 -0.20060382206465732 2316 38-39 -0.4265578363021234 2316 40-41 -0.21417720673226626 2316 42-43 0.061283148823655154 2316 44-45 0.17384947082614133 2316 46-47 0.270227290833283 2316 48-49 0.46146584046539374 2316 50-51 0.3774543494845446 2316 52-53 0.48971502929417454 2316 54-55 0.9982085000132201 2316 56-57 1.0694405702006016 2316 58-59 1.3352823312494273 2316 60-61 1.2153667771462757 2316 62-63 1.624130486244841 2316 64-65 2.094615212837983 2316 66-67 2.3066440084837048 2316 68-69 2.355935375219506 2316 70-71 2.771912767651763 2316 72-73 3.241915631561344 2316 74-75 3.5218082758862295 2316 76-77 3.755100304672723 2316 78-79 3.761280790152739 2316 80-81 4.00201929094165 2316 82-83 4.11848521280594 2316 84-85 4.383739568686291 2316 86-87 4.618057381904837 2316 88-89 4.9955542366560834 2316 90-91 5.018810107465917 2316 92-93 5.218472906030268 2316 94-95 5.0573399879472305 2316 96-97 5.156075592023294 2316 98-99 5.224587406219115 2316 100-101 4.855941567972231 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7225.0 3 2575.0 4 2736.0 5 3244.0 6 3753.0 7 4694.0 8 5873.0 9 7729.0 10 9496.0 11 11949.0 12 14598.0 13 17945.0 14 21324.0 15 25818.0 16 30492.0 17 37638.0 18 38436.0 19 36219.0 20 38612.0 21 41676.0 22 45712.0 23 50674.0 24 55839.0 25 60561.0 26 64818.0 27 69158.0 28 74462.0 29 79842.0 30 85550.0 31 93692.0 32 104159.0 33 118855.0 34 140623.0 35 173739.0 36 205199.0 37 214444.0 38 111587.0 39 7218.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.99981611827576 18.479355011156123 14.9716689446516 22.549159925916516 2 15.621600027199783 19.57633505598622 40.62358334466658 24.178481572147422 3 19.59051925687388 24.100352443631216 29.439150810166925 26.86997748932798 4 12.894339123617367 16.66572208525792 36.52837994842585 33.91155884269886 5 13.866062267705406 37.08309792888172 33.730355931530845 15.320483871882033 6 32.05780027350734 35.76408456525795 17.19284157122646 14.985273590008244 7 28.578370651341828 33.025740948968156 19.939967537512025 18.45592086217799 8 26.69206983052384 34.466590227353436 19.146584031613386 19.694755910509336 9 26.551888939892816 16.980246367859213 19.93332653280969 36.53453815943828 10-11 25.28539811463811 26.552703404376192 26.353154355474988 21.80874412551071 12-13 26.34997559634297 23.93244331019846 27.042345244569034 22.675235848889532 14-15 23.112070766873302 25.132133029066317 25.856494735338458 25.899301468721923 16-17 22.145473581458674 28.396162772603876 26.56663920527731 22.89172444066014 18-19 22.155907631280694 26.592722893842087 29.371033245183227 21.88033622969399 20-21 22.842358735378692 25.554029261860283 29.817749197751226 21.7858628050098 22-23 22.678832337393594 25.411787317517298 29.755080992709665 22.154299352379443 24-25 22.374587532012615 25.630616673295126 29.826737466782927 22.168058327909336 26-27 21.897906402079617 25.975033438624067 29.769207372366473 22.357852786929843 28-29 22.02587312224349 26.329063649505287 29.587194632127613 22.057868596123612 30-31 22.163484460666204 25.85579273426012 29.501131264728226 22.47959154034545 32-33 21.88905182571899 25.953357561331547 29.538369262085073 22.61922135086439 34-35 22.624708991298135 26.040730237914595 29.270227274283627 22.064333496503647 36-37 22.63488288291692 25.636071598759052 29.339391360486932 22.389654157837093 38-39 22.19147758716104 26.13813648477385 29.129348136088133 22.541037791976972 40-41 22.61453307946461 25.94931045839201 29.193856628700466 22.242299833442917 42-43 22.28636088491681 25.936496349675668 29.049966617781624 22.727176147625897 44-45 22.308093272375565 26.197007604927176 29.03472619287629 22.460172929820974 46-47 22.753110532902674 26.439264072503903 28.336971859832012 22.470653534761407 48-49 22.48315833584173 26.47232206620984 28.34720918840118 22.69731040954725 50-51 22.613369732958983 26.736019222175187 28.162148622938798 22.48846242192703 52-53 22.844895962773972 26.544482571073537 28.066185163222677 22.544436302929814 54-55 22.540993474753797 26.81786012905078 28.091418600138525 22.549727796056903 56-57 22.571972952792034 26.84586240654806 27.998978281638742 22.583186359021163 58-59 22.68404696173353 26.46775482358662 27.955545221382767 22.892652993297084 60-61 22.39555816802759 26.727265001451812 28.21979702980815 22.657379800712448 62-63 22.42840233780962 26.66826719469928 28.374930537555514 22.528399929935585 64-65 22.320905000448533 26.748834991336206 28.18815775185197 22.74210225636328 66-67 22.3042357987373 26.70808787686003 27.96384929257782 23.02382703182485 68-69 22.57087044020703 26.453049107959608 28.143340238310227 22.83274021352313 70-71 22.606308634070515 26.451815944500794 27.861205706309768 23.080669715118926 72-73 22.601068198650104 26.507715673028187 27.78345829017862 23.10775783814309 74-75 22.47023184200201 26.9762850981916 27.618556803249934 22.934926256556455 76-77 22.620422454747448 26.603917276754224 27.63632895530788 23.13933131319045 78-79 22.76677313277102 26.724030886973598 27.547974665316367 22.961221314939014 80-81 22.87566115395193 26.757657346082887 27.247581129683557 23.119100370281622 82-83 22.82585857746473 26.752271417044117 27.314512941989687 23.107357063501468 84-85 22.925311546151793 26.705412723405566 27.27909672271625 23.09017900772639 86-87 22.91982733122789 26.668553524888 27.115946650854195 23.295672493029915 88-89 22.97020281916275 26.694283757939225 26.954619507472906 23.380893915425116 90-91 22.8883242942311 26.658351212033 26.962991504872992 23.49033298886291 92-93 23.07036173841438 26.23460491698318 27.035380596859888 23.65965274774256 94-95 22.907279189054158 26.18824098306311 27.094491661889304 23.809988165993428 96-97 23.028882431325957 25.966234102291196 27.083925547678252 23.92095791870459 98-99 22.901947618545073 26.045174890918204 26.702487001199128 24.350390489337595 100-101 23.607292976493945 25.01128996772641 26.868303560351286 24.51311349542836 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1524.0 1 1014.5 2 717.0 3 899.0 4 702.5 5 627.5 6 1162.5 7 2601.5 8 4376.0 9 5051.0 10 4034.5 11 2874.0 12 2632.0 13 2701.0 14 2882.5 15 3042.5 16 3203.0 17 3430.5 18 3638.0 19 3931.0 20 4404.5 21 5071.5 22 5877.5 23 7016.0 24 8661.0 25 10664.5 26 13215.0 27 16176.5 28 18426.0 29 20622.5 30 23598.5 31 27327.5 32 31913.0 33 35021.5 34 36804.5 35 39120.0 36 42616.5 37 46604.0 38 49670.5 39 52260.0 40 55233.0 41 58551.5 42 60573.5 43 62679.0 44 65533.0 45 68404.0 46 70293.5 47 72833.0 48 75485.5 49 75681.0 50 75127.0 51 73971.5 52 72789.5 53 72808.0 54 73562.0 55 71227.5 56 66944.5 57 63131.5 58 59541.5 59 54178.0 60 46058.0 61 38698.0 62 32322.5 63 25475.0 64 19514.0 65 15491.0 66 11922.0 67 9027.0 68 6935.5 69 5153.0 70 3894.0 71 2839.0 72 2054.5 73 1499.0 74 1070.0 75 765.0 76 541.0 77 354.0 78 212.5 79 142.5 80 96.5 81 57.0 82 36.0 83 24.5 84 20.5 85 17.0 86 10.5 87 6.5 88 5.0 89 4.5 90 6.0 91 5.0 92 1.0 93 1.5 94 1.5 95 0.5 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3830202085295527 2 0.023652548314224812 3 0.04470850948816546 4 0.03894880710426241 5 0.058588448019866256 6 0.05759702383903049 7 0.03040367487896363 8 0.027429402336456316 9 0.032150469864245704 10-11 0.030238437515491 12-13 0.030899386969381513 14-15 0.023959417703531122 16-17 0.030521701567158364 18-19 0.026674031532010017 20-21 0.039444519194680304 22-23 0.026768452882565803 24-25 0.048674206211508544 26-27 0.040270706012043446 28-29 0.03200883783841202 30-31 0.039869415272181345 32-33 0.03682432671675719 34-35 0.035195558419669856 36-37 0.03559684915953196 38-39 0.017043053775319675 40-41 0.01286490901322607 42-43 0.01279409300030923 44-45 0.009253292354467191 46-47 0.015131021426564976 48-49 0.01310096238961554 50-51 0.00918247634155035 52-53 0.003635221996397825 54-55 0.003965696723343082 56-57 0.007766156083213536 58-59 0.0027146138284788956 60-61 0.00566528103334726 62-63 0.005382016981679897 64-65 0.007317654668073545 66-67 0.00413093408681571 68-69 0.006019361097931464 70-71 0.017420739177542825 72-73 0.014186807921007098 74-75 0.010103084509469281 76-77 0.007695340070296695 78-79 0.00566528103334726 80-81 0.008615948238215626 82-83 0.010103084509469281 84-85 0.004721067527789383 86-87 2.596587140284161E-4 88-89 0.024313497768115325 90-91 0.019899299629632255 92-93 0.01326619975308817 94-95 0.025446553974784777 96-97 0.01921474483810279 98-99 0.01763318721629335 100-101 0.11087427089013367 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2118165.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.07185193088408 #Duplication Level Percentage of deduplicated Percentage of total 1 78.77369645339847 44.95760740036766 2 12.244819924203894 13.976690992690077 3 3.7404173951158035 6.404176432012568 4 1.6770111921233966 3.828405377732075 5 0.8933903718895065 2.549372151047769 6 0.5888980365932179 2.016570092810189 7 0.37858283953786576 1.5124496633175104 8 0.2704591419152387 1.2348483280592373 9 0.19364627634836198 0.9946576449648697 >10 1.116842993053958 11.584755058845184 >50 0.07865357639343737 3.107473099715256 >100 0.039000664379840906 4.1531970434198655 >500 0.0029985611217362117 1.247467676826914 >1k 0.0013326938318827515 1.1005781439637436 >5k 8.329336449267197E-5 0.29092684178723704 >10k+ 1.6658672898534394E-4 1.0408240524397965 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11825 0.5582662351610946 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10070 0.47541150004839094 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6120 0.2889293327007103 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2675 0.126288556368366 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 9.442135055578767E-5 7 0.0 0.0 0.0 0.0 9.442135055578767E-5 8 0.0 0.0 0.0 4.7210675277893834E-5 1.8884270111157534E-4 9 0.0 0.0 0.0 4.7210675277893834E-5 1.8884270111157534E-4 10-11 0.0 0.0 0.0 4.7210675277893834E-5 1.8884270111157534E-4 12-13 0.0 0.0 0.0 4.7210675277893834E-5 1.8884270111157534E-4 14-15 2.3605337638946917E-5 0.0 0.0 4.7210675277893834E-5 2.360533763894692E-4 16-17 7.081601291684075E-5 0.0 0.0 4.7210675277893834E-5 2.83264051667363E-4 18-19 1.180266881947346E-4 0.0 0.0 9.442135055578767E-5 3.3047472694525685E-4 20-21 1.416320258336815E-4 0.0 0.0 1.6523736347262842E-4 3.3047472694525685E-4 22-23 1.416320258336815E-4 0.0 0.0 4.485014151399914E-4 3.5408006458420376E-4 24-25 1.8884270111157534E-4 0.0 0.0 8.025814797241952E-4 3.776854022231507E-4 26-27 1.8884270111157534E-4 0.0 0.0 0.0012274775572252397 3.776854022231507E-4 28-29 2.360533763894692E-4 0.0 0.0 0.003257536594174675 3.776854022231507E-4 30-31 2.360533763894692E-4 0.0 0.0 0.00989063647071876 3.776854022231507E-4 32-33 2.360533763894692E-4 0.0 0.0 0.022637518795750093 3.776854022231507E-4 34-35 2.360533763894692E-4 0.0 0.0 0.04180505295857499 3.776854022231507E-4 36-37 2.360533763894692E-4 0.0 0.0 0.07489973632837857 3.776854022231507E-4 38-39 3.776854022231507E-4 0.0 0.0 0.124069654630305 3.776854022231507E-4 40-41 3.776854022231507E-4 0.0 0.0 0.17836193119988292 3.776854022231507E-4 42-43 4.485014151399914E-4 0.0 0.0 0.23277223445765555 3.776854022231507E-4 44-45 5.193174280568322E-4 0.0 0.0 0.2993392865994859 4.485014151399914E-4 46-47 5.193174280568322E-4 0.0 0.0 0.37574976453675707 5.193174280568322E-4 48-49 5.193174280568322E-4 0.0 0.0 0.4563147818984829 5.193174280568322E-4 50-51 5.193174280568322E-4 0.0 0.0 0.5450000354080065 5.193174280568322E-4 52-53 5.193174280568322E-4 0.0 0.0 0.6258483168213996 5.66528103334726E-4 54-55 5.193174280568322E-4 0.0 0.0 0.7159026799139822 5.66528103334726E-4 56-57 5.66528103334726E-4 0.0 0.0 0.821371328484797 5.66528103334726E-4 58-59 5.66528103334726E-4 0.0 0.0 0.9319151246479854 5.66528103334726E-4 60-61 5.66528103334726E-4 0.0 0.0 1.056126411304124 5.66528103334726E-4 62-63 5.66528103334726E-4 0.0 0.0 1.1747904436151102 5.66528103334726E-4 64-65 5.66528103334726E-4 0.0 0.0 1.2969480658966606 5.66528103334726E-4 66-67 5.66528103334726E-4 0.0 0.0 1.4365264273557536 5.66528103334726E-4 68-69 6.137387786126199E-4 0.0 0.0 1.5968302752618422 5.66528103334726E-4 70-71 6.137387786126199E-4 0.0 0.0 1.764074092433781 6.137387786126199E-4 72-73 6.137387786126199E-4 0.0 0.0 1.930916618865858 6.137387786126199E-4 74-75 6.137387786126199E-4 0.0 0.0 2.0998128096725233 6.137387786126199E-4 76-77 6.137387786126199E-4 0.0 0.0 2.2758142071085112 6.137387786126199E-4 78-79 6.137387786126199E-4 0.0 0.0 2.4764595770395603 6.137387786126199E-4 80-81 7.317654668073544E-4 0.0 0.0 2.6864526606756316 6.373441162515667E-4 82-83 7.553708044463013E-4 0.0 0.0 2.8989243047637934 7.081601291684075E-4 84-85 7.789761420852483E-4 4.7210675277893834E-5 0.0 3.110357313995841 7.081601291684075E-4 86-87 8.025814797241953E-4 4.7210675277893834E-5 0.0 3.3266766281191504 7.081601291684075E-4 88-89 8.025814797241953E-4 4.7210675277893834E-5 0.0 3.560275049394169 7.081601291684075E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6610 0.0 46.773754 1 GTATCAA 10725 0.0 37.23547 1 TATCAAC 13265 0.0 30.130085 2 ATCAACG 13380 0.0 29.800083 3 TCAACGC 13550 0.0 29.531427 4 CAACGCA 13665 0.0 29.178566 5 AACGCAG 14065 0.0 28.344059 6 ACGCAGA 15685 0.0 25.198576 7 CGCAGAG 15795 0.0 24.932863 8 TGGTATC 3365 0.0 24.291538 2 GAGTACT 9520 0.0 24.09998 12-13 GCAGAGT 16710 0.0 23.141163 9 GTGGTAT 3605 0.0 23.028872 1 GTACTTT 10095 0.0 22.446003 14-15 TACATGG 8825 0.0 22.294476 2 GTACATG 9155 0.0 21.888683 1 CAGAGTA 15805 0.0 21.309515 10-11 TAAGGTG 1115 0.0 20.884922 5 AGTACTT 9815 0.0 20.568617 12-13 AGAGTAC 14280 0.0 20.458263 10-11 >>END_MODULE