FastQCFastQC Report
Thu 26 May 2016
SRR938147_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938147_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2118165
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116680.5508541591424653No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104410.49292666057648954No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64610.3050281729704721No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27400.1293572502614291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65100.048.662691
GTATCAA106900.036.5315671
TATCAAC133700.029.0665512
ATCAACG134400.028.8001373
TCAACGC136350.028.4580044
CAACGCA138750.027.9657555
AACGCAG142250.027.2441886
TAAGGTG11200.024.6251775
ACGCAGA160600.024.0425747
CGCAGAG161250.023.85738
GAGTACT97250.023.09976412-13
CGTACAC2103.4098775E-822.643843
GTACTTT101400.021.94358614-15
GCAGAGT174550.021.9034259
TGGTATC34650.021.6898562
GTACATG97650.021.3843041
TACATGG95700.021.37272
GTAAGGT9450.021.1342534
GTGGTAT37700.020.944481
GGACCGA8250.020.7245226
Produced by FastQC (version 0.11.5)