FastQCFastQC Report
Thu 26 May 2016
SRR938146_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938146_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2115882
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121070.5721963701189386No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103650.489866637175419No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61820.29217130255846024No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26800.12666112760541468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65200.049.634171
GTATCAA106200.036.225071
TATCAAC131550.028.6508712
ATCAACG132250.028.535163
TCAACGC133900.028.2190284
CAACGCA136350.027.6074035
AACGCAG140700.026.782586
ACGCAGA158000.023.7497147
GAGTACT96500.023.50582712-13
GTACATG98350.023.4892771
CGCAGAG159400.023.4815258
TACATGG96750.023.3836062
GTAAGGT8750.022.8137534
GTACTTT100250.022.37069914-15
TAAGGTG10200.021.9004575
ACATGGG99500.021.877513
TGGTATC33500.021.8489912
GTACCTG16700.021.72181
GCAGAGT172200.021.2395849
CAGAGTA163050.020.68432610-11
Produced by FastQC (version 0.11.5)