##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938146_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2115882 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.695615350950572 33.0 31.0 34.0 30.0 34.0 2 31.89903926589479 33.0 31.0 34.0 30.0 34.0 3 31.967755290701465 34.0 31.0 34.0 30.0 34.0 4 35.49905193200755 37.0 35.0 37.0 33.0 37.0 5 35.50255968905638 37.0 35.0 37.0 33.0 37.0 6 35.524875678322324 37.0 35.0 37.0 33.0 37.0 7 35.46351450600742 37.0 35.0 37.0 33.0 37.0 8 35.43304494295996 37.0 35.0 37.0 33.0 37.0 9 37.03765096541301 39.0 37.0 39.0 33.0 39.0 10-11 36.9366956663935 39.0 37.0 39.0 33.0 39.0 12-13 36.81880818495549 39.0 37.0 39.0 33.0 39.0 14-15 37.944950616338716 40.0 38.0 41.0 33.0 41.0 16-17 37.77758542300563 40.0 38.0 41.0 32.5 41.0 18-19 37.938866864976404 40.0 38.0 41.0 33.0 41.0 20-21 37.91744790115895 40.0 38.0 41.0 33.0 41.0 22-23 37.81691724774822 40.0 38.0 41.0 32.5 41.0 24-25 37.69011575314691 40.0 38.0 41.0 32.0 41.0 26-27 37.49540971566467 40.0 37.0 41.0 32.0 41.0 28-29 37.265032501812485 40.0 37.0 41.0 31.5 41.0 30-31 37.02262106298933 40.0 37.0 41.0 31.0 41.0 32-33 36.733987292297016 39.0 36.0 41.0 30.0 41.0 34-35 36.47223876378739 39.0 35.5 41.0 30.0 41.0 36-37 36.17754676300474 39.0 35.0 40.5 29.5 41.0 38-39 35.85954037134396 39.0 35.0 40.0 28.5 41.0 40-41 35.607852186464086 39.0 35.0 40.0 27.0 41.0 42-43 35.387110198016714 38.5 35.0 40.0 26.5 41.0 44-45 35.06429611859262 38.0 34.5 40.0 25.0 41.0 46-47 34.619128335134 38.0 34.0 40.0 23.5 41.0 48-49 34.17649235637904 38.0 33.5 40.0 22.0 41.0 50-51 33.46473007473952 37.5 32.5 39.5 20.0 40.5 52-53 33.59378264005271 38.0 33.0 39.5 20.5 40.5 54-55 33.67009880513186 38.0 33.0 40.0 19.5 41.0 56-57 33.32679941509026 37.5 33.0 40.0 17.0 41.0 58-59 32.97312562798871 37.0 32.5 40.0 13.5 41.0 60-61 32.50912503627329 36.5 32.0 39.5 8.5 41.0 62-63 31.92590087727009 36.0 31.0 39.0 7.0 41.0 64-65 31.368185938535326 35.0 30.5 39.0 2.0 40.0 66-67 30.784807234051804 35.0 29.5 38.5 2.0 40.0 68-69 30.13440470687874 35.0 29.0 38.0 2.0 40.0 70-71 29.536073609019788 34.0 28.5 37.0 2.0 39.5 72-73 28.846666780094544 34.0 27.0 36.5 2.0 39.0 74-75 28.10950516144095 34.0 26.0 36.0 2.0 39.0 76-77 27.402163967555847 33.0 25.5 35.0 2.0 38.0 78-79 26.63487968610726 33.0 23.5 35.0 2.0 37.0 80-81 25.921871115686038 32.0 21.0 35.0 2.0 36.5 82-83 25.143563771514668 31.5 18.5 35.0 2.0 36.0 84-85 24.436620756734072 31.0 12.5 34.0 2.0 35.5 86-87 23.698830085987783 31.0 6.0 34.0 2.0 35.0 88-89 22.827350485518572 30.0 2.0 34.0 2.0 35.0 90-91 21.93960580032346 29.0 2.0 34.0 2.0 35.0 92-93 20.989176853907733 29.0 2.0 34.0 2.0 35.0 94-95 19.986015760803298 27.0 2.0 34.0 2.0 35.0 96-97 18.73905775463849 25.5 2.0 33.0 2.0 35.0 98-99 17.239028925053475 21.0 2.0 32.5 2.0 34.0 100-101 13.859416309605166 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.48647705434741795 1101 2 0.45501737786623764 1101 3 0.3843293077955323 1101 4 0.2613483747520391 1101 5 0.30021457275065444 1101 6 0.2659198395901541 1101 7 0.24854718103909335 1101 8 0.15909610874192737 1101 9 0.3015209555706022 1101 10-11 0.4371364659903705 1101 12-13 0.5405799648998411 1101 14-15 0.6981863018380849 1101 16-17 0.8016906207686461 1101 18-19 0.5628092780786247 1101 20-21 0.6956269604608778 1101 22-23 0.7193960081402437 1101 24-25 0.7493528333567809 1101 26-27 0.8266427248719239 1101 28-29 0.8696577757212225 1101 30-31 0.8701120328813232 1101 32-33 0.8303739966432957 1101 34-35 0.8639056345611777 1101 36-37 0.9492902375879879 1101 38-39 1.0234561917494247 1101 40-41 1.2053648155704408 1101 42-43 1.3744539626452905 1101 44-45 1.117724242525263 1101 46-47 1.3676087208155394 1101 48-49 1.3296295398237348 1101 50-51 1.2837059617252535 1101 52-53 1.4278546615010725 1101 54-55 1.628581003782358 1101 56-57 1.6089705359837083 1101 58-59 1.663820454142794 1101 60-61 1.7290355248631641 1101 62-63 1.6378655238762256 1101 64-65 1.8416692512502486 1101 66-67 1.9026154336094372 1101 68-69 1.878944664155231 1101 70-71 2.2625581339993133 1101 72-73 2.285154806490347 1101 74-75 2.3703818472453833 1101 76-77 2.4976802274246985 1101 78-79 2.586269632723827 1101 80-81 2.9774777164889024 1101 82-83 3.130987597742603 1101 84-85 3.3109116707698725 1101 86-87 3.664588698995736 1101 88-89 4.057304986014827 1101 90-91 4.40865689290839 1101 92-93 4.5566481207187515 1101 94-95 4.95529029667966 1101 96-97 5.122376408723369 1101 98-99 5.207047635492454 1101 100-101 4.94001678359524 1102 1 0.4808750777026489 1102 2 0.3897130806801563 1102 3 0.32908480107248295 1102 4 0.23549587066030853 1102 5 0.3735195615596538 1102 6 0.39993304350514336 1102 7 0.2571231925376125 1102 8 0.3407349847082557 1102 9 0.5390793464546988 1102 10-11 0.44634200640806654 1102 12-13 0.5243580846091689 1102 14-15 0.6019345544764505 1102 16-17 0.6703967396038806 1102 18-19 0.48179379203330797 1102 20-21 0.5669410271022386 1102 22-23 0.5857450895996834 1102 24-25 0.5598505484103882 1102 26-27 0.5933405183244815 1102 28-29 0.6460349107127286 1102 30-31 0.6697152790033343 1102 32-33 0.6243361229669873 1102 34-35 0.76949262979754 1102 36-37 0.8536742144648031 1102 38-39 0.7915185042706128 1102 40-41 1.0370869904203772 1102 42-43 1.1219150081673632 1102 44-45 1.1135554966719 1102 46-47 1.1746715537435506 1102 48-49 1.2649975755808711 1102 50-51 1.3087640569236498 1102 52-53 1.299092413073275 1102 54-55 1.440399166656178 1102 56-57 1.4043209770341178 1102 58-59 1.6366834069220673 1102 60-61 1.7719389556056981 1102 62-63 1.6315802316768107 1102 64-65 1.786232126419069 1102 66-67 2.033167425291939 1102 68-69 1.9902903212927505 1102 70-71 2.089807992351787 1102 72-73 2.3882299940113967 1102 74-75 2.5024763326227415 1102 76-77 2.7527115306833245 1102 78-79 2.9788356156900804 1102 80-81 3.1479961351730843 1102 82-83 3.389629364106977 1102 84-85 3.455062810652297 1102 86-87 3.4755381091450204 1102 88-89 3.8391717370880265 1102 90-91 4.168834253634799 1102 92-93 4.5681664864611164 1102 94-95 4.951810780094789 1102 96-97 5.038343098181301 1102 98-99 5.196509397418726 1102 100-101 4.819675522881564 1103 1 0.6464118369848322 1103 2 0.5231092184965114 1103 3 0.5443856216737402 1103 4 0.4832459265580269 1103 5 0.4778427188576231 1103 6 0.43241829997427317 1103 7 0.40946841426415403 1103 8 0.5002250547094036 1103 9 0.6450270261881812 1103 10-11 0.6994991323857889 1103 12-13 0.6613889534560897 1103 14-15 0.9150038147540869 1103 16-17 0.9559846035191342 1103 18-19 0.9164831873027168 1103 20-21 0.9111802842742591 1103 22-23 0.9882047840168227 1103 24-25 0.9773495691861669 1103 26-27 1.0296758540239566 1103 28-29 1.101584296876247 1103 30-31 1.1134372267038373 1103 32-33 1.2608315419500116 1103 34-35 1.3089328932258937 1103 36-37 1.3651711343300263 1103 38-39 1.4123322193158785 1103 40-41 1.3802727094208294 1103 42-43 1.5391093092933374 1103 44-45 1.6316311482738186 1103 46-47 1.563018736839176 1103 48-49 1.5876117975835768 1103 50-51 1.3847667841482902 1103 52-53 1.570376626291356 1103 54-55 1.5132615615579184 1103 56-57 1.645079680284887 1103 58-59 1.7694907121456893 1103 60-61 1.7368369718775298 1103 62-63 1.9244495647107236 1103 64-65 2.095839622197939 1103 66-67 2.415125555708702 1103 68-69 2.597916879735809 1103 70-71 2.6416789677564623 1103 72-73 2.7993782548864665 1103 74-75 2.8127748832410866 1103 76-77 3.0296903343325603 1103 78-79 3.340019102675889 1103 80-81 3.5851756968246242 1103 82-83 3.7144103732255225 1103 84-85 3.4435975310063043 1103 86-87 3.540369405118664 1103 88-89 3.825981497592661 1103 90-91 4.013698689087288 1103 92-93 4.456206678301445 1103 94-95 4.705795906271113 1103 96-97 4.99767016426328 1103 98-99 5.200844918740827 1103 100-101 4.735539149967018 1104 1 0.2603871878595001 1104 2 0.1590139794509433 1104 3 0.22860986449996545 1104 4 0.2059743758889141 1104 5 0.20694916018344856 1104 6 0.19257885914326778 1104 7 0.23041007494863663 1104 8 0.16251489822965937 1104 9 0.3277160547623623 1104 10-11 0.29904197252335507 1104 12-13 0.4358810649024818 1104 14-15 0.5210166540874965 1104 16-17 0.7350876404317788 1104 18-19 0.5769813116471525 1104 20-21 0.6057648020596744 1104 22-23 0.5308962719860375 1104 24-25 0.5825195227746249 1104 26-27 0.6003394387184215 1104 28-29 0.6511277533299875 1104 30-31 0.8225197021348336 1104 32-33 0.9204663834188196 1104 34-35 0.8491200831234735 1104 36-37 0.9157614478353935 1104 38-39 0.8225541753155952 1104 40-41 0.9242299934269482 1104 42-43 0.9441291165213741 1104 44-45 1.060002882018189 1104 46-47 1.040697672502219 1104 48-49 1.284023692837998 1104 50-51 1.313896440050165 1104 52-53 1.136590554919593 1104 54-55 1.4055801195424138 1104 56-57 1.4323194114301572 1104 58-59 1.4881294634069349 1104 60-61 1.5446714950923948 1104 62-63 1.7185042853698143 1104 64-65 1.7412840771425913 1104 66-67 1.8892942772517678 1104 68-69 2.0002408906510603 1104 70-71 2.3135249403650278 1104 72-73 2.359619933003895 1104 74-75 2.5150149284141285 1104 76-77 2.6785483004539508 1104 78-79 2.8374113515314505 1104 80-81 2.9384945007486216 1104 82-83 3.200029368911899 1104 84-85 3.242705750844525 1104 86-87 3.3547952831197563 1104 88-89 3.6363914887235786 1104 90-91 3.8442843772864776 1104 92-93 4.110035381143717 1104 94-95 4.336812839704859 1104 96-97 4.545661971366879 1104 98-99 4.509591149673913 1104 100-101 4.622355618639666 1105 1 0.24349474175349783 1105 2 0.3176810457429333 1105 3 0.17245511727924168 1105 4 0.10885212157646151 1105 5 0.11479665464795374 1105 6 0.23121157302779238 1105 7 0.30117833985057274 1105 8 0.2418455494622762 1105 9 0.34370491018164273 1105 10-11 0.2889809912362793 1105 12-13 0.27946122598813616 1105 14-15 0.3766376709506858 1105 16-17 0.5216569043378954 1105 18-19 0.4851185492489378 1105 20-21 0.49561164388165224 1105 22-23 0.4679264646840693 1105 24-25 0.39760908820868934 1105 26-27 0.467432022624223 1105 28-29 0.47955547893281647 1105 30-31 0.5875034348422403 1105 32-33 0.6259488024170992 1105 34-35 0.6826142124442285 1105 36-37 0.5874547625944189 1105 38-39 0.7381125615505155 1105 40-41 0.9506396259342296 1105 42-43 1.0040851074872421 1105 44-45 1.0434480631486593 1105 46-47 1.251641760722464 1105 48-49 1.217397180831064 1105 50-51 1.2804321052800063 1105 52-53 1.3162020375265797 1105 54-55 1.4708179926726075 1105 56-57 1.5020091756719154 1105 58-59 1.541537525493105 1105 60-61 1.55221634431512 1105 62-63 1.5750687963357244 1105 64-65 1.6392658953727768 1105 66-67 1.7991227546349542 1105 68-69 1.9778987463478934 1105 70-71 2.1760102315225183 1105 72-73 2.1839371633876006 1105 74-75 2.3019603928588523 1105 76-77 2.4095802116590974 1105 78-79 2.429974418880434 1105 80-81 2.5259856222385615 1105 82-83 2.9123000240284505 1105 84-85 3.026742477948609 1105 86-87 3.273147573531496 1105 88-89 3.549310929047131 1105 90-91 3.505263846535577 1105 92-93 3.7099947683336545 1105 94-95 4.032398546478596 1105 96-97 4.082029944707852 1105 98-99 4.15263735871638 1105 100-101 4.0532901211726475 1106 1 0.27091963844793554 1106 2 0.25580236571880377 1106 3 0.13364120135515378 1106 4 0.15861631892846617 1106 5 0.4132322051676738 1106 6 0.1824334005037187 1106 7 0.2894803229281351 1106 8 0.2718263263028007 1106 9 0.2672492802177331 1106 10-11 0.2425843065285349 1106 12-13 0.3962651648978479 1106 14-15 0.44398251799790955 1106 16-17 0.2255571082461003 1106 18-19 0.3270151982781542 1106 20-21 0.16099471236497465 1106 22-23 0.14892980981653636 1106 24-25 0.14203393949409104 1106 26-27 0.23997112304954982 1106 28-29 0.3130631325180744 1106 30-31 0.38145473473002056 1106 32-33 0.22534572714405243 1106 34-35 0.42830121875608995 1106 36-37 0.43451333809127135 1106 38-39 0.11948815267889046 1106 40-41 0.7166606914032485 1106 42-43 0.5722115305740587 1106 44-45 0.8014982206747803 1106 46-47 1.1803133517470457 1106 48-49 1.1090873268535475 1106 50-51 0.8813909928018191 1106 52-53 1.0118452565516733 1106 54-55 0.5339657471956656 1106 56-57 1.0209491773585313 1106 58-59 1.2215984168508953 1106 60-61 1.3374803546251357 1106 62-63 1.3219311399178046 1106 64-65 1.1981513629669358 1106 66-67 1.3502420874248209 1106 68-69 1.1901834047962403 1106 70-71 1.553288197543587 1106 72-73 1.761256646243524 1106 74-75 1.8039687697452216 1106 76-77 2.092987316659933 1106 78-79 2.326484734300262 1106 80-81 2.3271315517507567 1106 82-83 2.5271991619054255 1106 84-85 2.6867393858093465 1106 86-87 2.7119198645039653 1106 88-89 3.0446189861241315 1106 90-91 3.3904875292950614 1106 92-93 3.717398603883744 1106 94-95 3.8206087384290868 1106 96-97 3.7705662167988088 1106 98-99 3.867730674289735 1106 100-101 3.976238011057891 1107 1 0.2083531721061327 1107 2 0.06608456023200304 1107 3 -4.1075219979092026E-4 1107 4 -0.0022123335552635126 1107 5 0.13948027383276695 1107 6 0.1241796889818545 1107 7 -0.025459295403912563 1107 8 0.06620827329508217 1107 9 0.025940674861153923 1107 10-11 0.05431132041347553 1107 12-13 0.11880972124632194 1107 14-15 0.282833483536173 1107 16-17 0.29602409081628167 1107 18-19 0.16670432441046756 1107 20-21 0.2773068057511452 1107 22-23 0.20520232052608378 1107 24-25 0.33150267383025067 1107 26-27 0.4039751743313431 1107 28-29 0.35251713961815767 1107 30-31 0.44412506684211195 1107 32-33 0.4458615421286325 1107 34-35 0.41691732363468503 1107 36-37 0.5295587858250883 1107 38-39 0.6004770995006439 1107 40-41 0.6007640610982108 1107 42-43 0.5149701227536383 1107 44-45 0.5962672097662889 1107 46-47 0.6470359991356744 1107 48-49 0.8347757605474015 1107 50-51 0.5268599131662342 1107 52-53 0.6439180762748009 1107 54-55 0.7275741267346234 1107 56-57 0.8474313315656161 1107 58-59 0.7363452892510267 1107 60-61 0.8997367738914548 1107 62-63 0.9409359921545324 1107 64-65 1.0171634496354294 1107 66-67 1.1576010547613613 1107 68-69 1.2027129238615508 1107 70-71 1.455909645566777 1107 72-73 1.6368807451692113 1107 74-75 1.78530496929152 1107 76-77 1.950411135509757 1107 78-79 1.9759775489592855 1107 80-81 2.2652570071324014 1107 82-83 2.4155000522572756 1107 84-85 2.6615873335560813 1107 86-87 2.7834358396795267 1107 88-89 2.9661383201430134 1107 90-91 3.2541401938913026 1107 92-93 3.3578824840195445 1107 94-95 3.649112399568306 1107 96-97 3.7761289780780594 1107 98-99 3.769691722042154 1107 100-101 3.6441496889809883 1108 1 0.16692055064026334 1108 2 0.1607234193416609 1108 3 0.12581426541528629 1108 4 0.0868719852615456 1108 5 0.09778652896947904 1108 6 0.14864339410004845 1108 7 0.07277274674327572 1108 8 0.08324481591184707 1108 9 0.13765301694844112 1108 10-11 0.06992849318589123 1108 12-13 0.11402613996629185 1108 14-15 0.3361249380970932 1108 16-17 0.3034743166117977 1108 18-19 0.3224355041272773 1108 20-21 0.2950494174346616 1108 22-23 0.21258902453703854 1108 24-25 0.2413187440599387 1108 26-27 0.3192819698918541 1108 28-29 0.44939098828580626 1108 30-31 0.45074032321115 1108 32-33 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-3.3374114811705624 2316 80-81 -3.3287552808310714 2316 82-83 -3.537021186414268 2316 84-85 -3.495565003573251 2316 86-87 -3.485626612170144 2316 88-89 -3.8771492616249965 2316 90-91 -3.8606079011066683 2316 92-93 -4.250950562162746 2316 94-95 -4.247759580092996 2316 96-97 -4.523483017831788 2316 98-99 -4.60933478339971 2316 100-101 -4.093605595298541 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7465.0 3 2474.0 4 2717.0 5 2908.0 6 3325.0 7 4098.0 8 5136.0 9 6318.0 10 7878.0 11 9608.0 12 11972.0 13 14123.0 14 16401.0 15 19548.0 16 23586.0 17 28416.0 18 28257.0 19 23626.0 20 24317.0 21 25658.0 22 28358.0 23 31121.0 24 34786.0 25 38714.0 26 43363.0 27 49302.0 28 56779.0 29 65010.0 30 75684.0 31 90259.0 32 108243.0 33 133131.0 34 170003.0 35 223958.0 36 275203.0 37 283518.0 38 133746.0 39 6873.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.01194775917792 18.450893541781312 15.006772960764305 22.53038573827646 2 15.59131625046986 19.612101267857692 40.59716735579685 24.199415125875596 3 19.565050857556283 24.104306459926324 29.48967480482142 26.84096787769597 4 12.935820033735872 16.65499420002147 36.48382744673738 33.925358319505285 5 13.85320393566444 37.08788077023683 33.73094877591111 15.327966518187619 6 32.144652901056666 35.72691852690661 17.129580357493392 14.998848214543337 7 28.6279062070009 33.000609966570046 19.925763758528895 18.445720067900155 8 26.751265003446782 34.45967733546917 19.124979787367838 19.664077873716202 9 26.559131040013316 16.998093523274903 19.967884945642716 36.47489049106906 10-11 25.28934123445955 26.557709366920058 26.332132906879313 21.82081649174108 12-13 26.366142600697763 23.961188809618207 27.00922097937709 22.66344761030694 14-15 23.152093664621706 25.14558266279691 25.845594553850276 25.856729118731103 16-17 22.168989372000777 28.424206505214002 26.599122989711947 22.807681133073274 18-19 22.171958431892683 26.58207407880256 29.383582938209347 21.862384551095413 20-21 22.825524887978737 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29.079578255547677 22.469158678384048 46-47 22.68681221534851 26.4410917380275 28.429031071627463 22.443064974996528 48-49 22.478019687023412 26.46969558011102 28.431212186250477 22.621072546615093 50-51 22.57201154425301 26.728713126825355 28.24572213386692 22.453553195054717 52-53 22.78164575062124 26.572532250920695 28.13845856410785 22.507363434350214 54-55 22.47146582386724 26.79535871012359 28.18000278422559 22.55317268178358 56-57 22.536498528383305 26.832844054954535 28.07411663410194 22.556540782560212 58-59 22.601738655276684 26.481877229628516 28.050647044281302 22.8657370708135 60-61 22.305315697694482 26.776693237355527 28.266419726124788 22.651571338825207 62-63 22.32396531622228 26.651519831946242 28.48856790561724 22.535946946214242 64-65 22.208792219127858 26.741914601094635 28.29720034857082 22.752092831206685 66-67 22.156385027531645 26.75602112157621 28.117371605456746 22.9702222454354 68-69 22.460611423396237 26.50428627880287 28.225628052342895 22.809474245458 70-71 22.457302132453027 26.60599312352855 27.910026804861037 23.026677939157388 72-73 22.42667982657659 26.622251970677603 27.873010650829393 23.078057551916412 74-75 22.266804407420512 27.049277770963222 27.75412377334981 22.92979404826646 76-77 22.465538393256168 26.661432660341273 27.77870079264 23.094328153762564 78-79 22.59142847486581 26.829237164656693 27.716183003718374 22.863151356759122 80-81 22.724675017726305 26.807397778303 27.470527062160244 22.99740014181045 82-83 22.62019023399325 26.840222589607205 27.556040325163284 22.983546851236262 84-85 22.70447231791385 26.857187019047153 27.465598281710275 22.972742381328725 86-87 22.707878468776727 26.826986951181958 27.30808486966188 23.157049710379432 88-89 22.727329672789555 26.918564838025784 27.099748281475627 23.25435720770903 90-91 22.657708011801255 26.82587473468802 27.132134407243573 23.384282846267148 92-93 22.83319518668233 26.467929296331306 27.208225173605076 23.490650343381287 94-95 22.724745541870885 26.354482193249655 27.31638568992441 23.604386574955054 96-97 22.860201527113077 26.106126393036256 27.269224650799874 23.764447429050794 98-99 22.724475006121896 26.28729983293608 26.93142248262469 24.056802678317336 100-101 23.478350594024796 25.224299058785277 26.99710826915745 24.30024207803248 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1540.0 1 1033.0 2 702.0 3 871.0 4 658.5 5 595.5 6 1205.5 7 2714.0 8 4478.5 9 5145.5 10 4170.0 11 2891.5 12 2543.5 13 2684.5 14 2855.0 15 2969.0 16 3111.5 17 3337.5 18 3602.0 19 3853.0 20 4274.0 21 4940.0 22 5722.0 23 6874.0 24 8322.0 25 10231.0 26 12880.0 27 15833.5 28 18146.0 29 20260.5 30 23174.0 31 27321.0 32 32379.5 33 35196.0 34 36778.5 35 39309.5 36 42930.5 37 47004.0 38 50330.5 39 52906.0 40 55506.0 41 59121.5 42 61464.0 43 63351.5 44 66209.5 45 69571.0 46 71416.5 47 74028.5 48 77289.5 49 77380.5 50 76065.5 51 74498.5 52 73686.0 53 74246.0 54 75315.0 55 72452.0 56 67441.0 57 63011.5 58 58993.0 59 53694.5 60 44833.5 61 37015.0 62 30781.5 63 23578.5 64 17544.5 65 13687.5 66 10327.5 67 7692.5 68 5823.5 69 4356.0 70 3274.0 71 2258.5 72 1565.5 73 1108.0 74 745.5 75 507.0 76 345.0 77 226.0 78 153.0 79 100.5 80 66.0 81 38.5 82 23.0 83 15.5 84 15.0 85 15.5 86 10.5 87 6.5 88 4.5 89 4.0 90 5.0 91 2.0 92 1.0 93 2.5 94 1.5 95 0.5 96 1.0 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4590520643400719 2 0.04239366845599141 3 0.05444538022441705 4 0.05761190841455242 5 0.0840784126903107 6 0.08379484300164186 7 0.04782875415547748 8 0.041023081627425344 9 0.046599952171245844 10-11 0.0509480207308347 12-13 0.04615096683085352 14-15 0.04000695690969534 16-17 0.0404323114426986 18-19 0.04912844856187632 20-21 0.05749375437760707 22-23 0.04879761725842935 24-25 0.06616626068939573 26-27 0.05806089375494475 28-29 0.04485127242445468 30-31 0.04683626024513654 32-33 0.046718106208191196 34-35 0.05061718942738773 36-37 0.045560196646126774 38-39 0.02819155321516039 40-41 0.026442873468369217 42-43 0.02556853359497363 44-45 0.015856271758065903 46-47 0.027222690112208524 48-49 0.027411736571321083 50-51 0.017510428275300798 52-53 0.01706144293490847 54-55 0.01855018380041987 56-57 0.016612457594516142 58-59 0.016021687409789393 60-61 0.018573814607808943 62-63 0.017415905045744518 64-65 0.018266614111751033 66-67 0.010657494132470524 68-69 0.013894914744773102 70-71 0.019259108022091968 72-73 0.017297751008799166 74-75 0.014816516232946827 76-77 0.010893802206361224 78-79 0.014532946544277989 80-81 0.018053936845249405 82-83 0.015572702069397064 84-85 0.013705868285660542 86-87 0.004560745826090491 88-89 0.02942035519939203 90-91 0.03230331370085855 92-93 0.017793997963969636 94-95 0.033130391959476 96-97 0.027577152223044576 98-99 0.024765086143745254 100-101 0.14809426990730107 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2115882.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.95684648943631 #Duplication Level Percentage of deduplicated Percentage of total 1 78.92611455333487 44.95382590621959 2 12.145850245464429 13.835786558291996 3 3.7385565789943795 6.388091794855652 4 1.639390737489926 3.7349810628567 5 0.9091547304219236 2.589129320789318 6 0.5346988118106928 1.8272854885431373 7 0.3654598682754962 1.4570809130791937 8 0.27601087319007844 1.2576567146962057 9 0.20867113684385824 1.0696724917192613 >10 1.1319334150655012 11.811795297706745 >50 0.0788588906219248 3.0961737800050178 >100 0.04078891066507504 4.310800432861796 >500 0.0029239569216589316 1.2119307542899351 >1k 0.001336666021329789 1.092355489840358 >5k 8.354162633311182E-5 0.29415610219946386 >10k+ 1.6708325266622364E-4 1.0692778920456734 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12107 0.5721963701189386 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10365 0.489866637175419 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6182 0.29217130255846024 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2680 0.12666112760541468 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 4.72616147781398E-5 0.0 0.0 0.0 0.0 6 4.72616147781398E-5 0.0 0.0 0.0 4.72616147781398E-5 7 9.45232295562796E-5 0.0 0.0 0.0 4.72616147781398E-5 8 9.45232295562796E-5 0.0 0.0 0.0 4.72616147781398E-5 9 9.45232295562796E-5 0.0 0.0 0.0 4.72616147781398E-5 10-11 9.45232295562796E-5 0.0 0.0 7.08924221672097E-5 4.72616147781398E-5 12-13 1.417848443344194E-4 0.0 0.0 9.45232295562796E-5 1.417848443344194E-4 14-15 2.835696886688388E-4 0.0 0.0 9.45232295562796E-5 1.417848443344194E-4 16-17 3.072004960579087E-4 0.0 0.0 9.45232295562796E-5 1.417848443344194E-4 18-19 3.544621108360485E-4 0.0 0.0 1.1815403694534951E-4 1.890464591125592E-4 20-21 3.780929182251184E-4 0.0 0.0 1.654156517234893E-4 2.3630807389069902E-4 22-23 4.253545330032582E-4 0.0 0.0 3.308313034469786E-4 2.3630807389069902E-4 24-25 4.7261614778139804E-4 0.0 0.0 4.017237256141883E-4 2.3630807389069902E-4 26-27 4.7261614778139804E-4 0.0 0.0 9.216014881737261E-4 2.835696886688388E-4 28-29 5.198777625595378E-4 0.0 0.0 0.0031665281901353667 3.308313034469786E-4 30-31 5.198777625595378E-4 0.0 0.0 0.00968863102951866 3.308313034469786E-4 32-33 5.198777625595378E-4 0.0 0.0 0.023347237700401063 3.780929182251184E-4 34-35 5.435085699486077E-4 0.0 0.0 0.043953301743670015 4.017237256141883E-4 36-37 5.671393773376776E-4 0.0 0.0 0.07932862040510766 4.253545330032582E-4 38-39 5.671393773376776E-4 0.0 0.0 0.12696832810147257 4.253545330032582E-4 40-41 5.671393773376776E-4 0.0 0.0 0.18186269366628197 4.7261614778139804E-4 42-43 5.671393773376776E-4 0.0 0.0 0.2367806900384804 5.198777625595378E-4 44-45 5.671393773376776E-4 0.0 0.0 0.3023798113505385 5.907701847267476E-4 46-47 5.671393773376776E-4 0.0 0.0 0.3828190797029324 6.144009921158174E-4 48-49 5.671393773376776E-4 0.0 0.0 0.46543238233512074 6.144009921158174E-4 50-51 5.671393773376776E-4 0.0 0.0 0.5522283378751746 6.616626068939572E-4 52-53 5.671393773376776E-4 0.0 0.0 0.6316278507024493 7.08924221672097E-4 54-55 5.671393773376776E-4 0.0 0.0 0.7205978405222975 7.08924221672097E-4 56-57 5.671393773376776E-4 0.0 0.0 0.829252292897241 7.08924221672097E-4 58-59 5.671393773376776E-4 0.0 0.0 0.9447596794150146 7.08924221672097E-4 60-61 5.671393773376776E-4 0.0 0.0 1.0718461615534327 7.08924221672097E-4 62-63 5.671393773376776E-4 0.0 0.0 1.1946318367470399 7.08924221672097E-4 64-65 5.671393773376776E-4 0.0 0.0 1.316472279645084 7.561858364502368E-4 66-67 5.671393773376776E-4 0.0 0.0 1.455728627588873 7.561858364502368E-4 68-69 5.671393773376776E-4 0.0 0.0 1.6133224820665801 7.561858364502368E-4 70-71 5.671393773376776E-4 0.0 0.0 1.7827317402388223 7.561858364502368E-4 72-73 5.907701847267476E-4 0.0 0.0 1.951550228226338 8.270782586174464E-4 74-75 6.144009921158174E-4 0.0 0.0 2.120179669754741 8.507090660065164E-4 76-77 6.616626068939572E-4 0.0 0.0 2.301002607895904 8.507090660065164E-4 78-79 6.616626068939572E-4 0.0 0.0 2.5013682237478267 8.507090660065164E-4 80-81 6.616626068939572E-4 0.0 0.0 2.7147780452785177 9.21601488173726E-4 82-83 6.616626068939572E-4 0.0 0.0 2.9365531726249383 0.0010397555251190757 84-85 6.616626068939572E-4 0.0 0.0 3.1558943268102855 0.0010397555251190757 86-87 6.616626068939572E-4 0.0 0.0 3.3827500777453565 0.0010397555251190757 88-89 6.616626068939572E-4 0.0 0.0 3.629290291235523 0.0010633863325081455 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6520 0.0 49.63417 1 GTATCAA 10620 0.0 36.22507 1 TATCAAC 13155 0.0 28.650871 2 ATCAACG 13225 0.0 28.53516 3 TCAACGC 13390 0.0 28.219028 4 CAACGCA 13635 0.0 27.607403 5 AACGCAG 14070 0.0 26.78258 6 ACGCAGA 15800 0.0 23.749714 7 GAGTACT 9650 0.0 23.505827 12-13 GTACATG 9835 0.0 23.489277 1 CGCAGAG 15940 0.0 23.481525 8 TACATGG 9675 0.0 23.383606 2 GTAAGGT 875 0.0 22.813753 4 GTACTTT 10025 0.0 22.370699 14-15 TAAGGTG 1020 0.0 21.900457 5 ACATGGG 9950 0.0 21.87751 3 TGGTATC 3350 0.0 21.848991 2 GTACCTG 1670 0.0 21.7218 1 GCAGAGT 17220 0.0 21.239584 9 CAGAGTA 16305 0.0 20.684326 10-11 >>END_MODULE