FastQCFastQC Report
Thu 26 May 2016
SRR938146_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938146_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2115882
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117680.5561746827091492No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105720.499649791434494No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62670.29618853981460214No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28040.132521567837904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64700.045.376241
GTATCAA104750.037.1427571
TATCAAC131400.029.4288522
ATCAACG131550.029.2140453
TCAACGC133650.028.8575534
CAACGCA135350.028.4249155
AACGCAG139300.027.5841466
ACGCAGA156350.024.2723087
GTGTAAG9100.024.013791
CGCAGAG157850.023.9212958
TACATGG94500.023.023852
GTACATG96450.023.0016461
GAGTACT96850.022.43642212-13
GCAGAGT170300.021.8935999
ACATGGG96450.021.8190353
GTACTTT101000.021.5145314-15
TGGTATC34500.020.7922822
AGAGTAC146750.020.6330910-11
AGTACTT101450.020.22524812-13
CAGAGTA163700.019.99091710-11
Produced by FastQC (version 0.11.5)