FastQCFastQC Report
Thu 26 May 2016
SRR938145_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938145_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2119069
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123280.5817649165742125No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104970.49535904682669607No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64990.3066912875418403No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26800.12647063403787231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66550.046.115441
GTATCAA107600.037.3493651
TATCAAC134600.029.6201882
ATCAACG136350.029.0657673
TCAACGC138400.028.6009064
CAACGCA139200.028.5048085
AACGCAG143850.027.6164156
ACGCAGA160100.024.7063267
CGCAGAG161450.024.4408448
GAGTACT93300.023.58895912-13
TAAGGTG10700.023.5376455
GTACTTT98050.022.2133414-15
GCAGAGT174050.022.2060939
TACATGG94700.022.1288832
GTACATG96550.021.7512281
AGAGTAC146700.020.78869810-11
AGTACTT97150.020.77037412-13
ACATGGG96700.020.6883833
GTACCTG18750.020.6189791
TGGTATC35450.020.5090752
Produced by FastQC (version 0.11.5)