##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938145_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2119069 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.744400017177355 33.0 31.0 34.0 30.0 34.0 2 31.943241583921996 33.0 31.0 34.0 30.0 34.0 3 32.00058752216186 34.0 31.0 34.0 30.0 34.0 4 35.53250602033251 37.0 35.0 37.0 33.0 37.0 5 35.54703551418099 37.0 35.0 37.0 33.0 37.0 6 35.57757864420649 37.0 35.0 37.0 33.0 37.0 7 35.51475435674818 37.0 35.0 37.0 33.0 37.0 8 35.495904569412325 37.0 35.0 37.0 33.0 37.0 9 37.13149359459272 39.0 37.0 39.0 34.0 39.0 10-11 37.04229215754654 39.0 37.0 39.0 33.0 39.0 12-13 36.935693457834546 39.0 37.0 39.0 33.0 39.0 14-15 38.14245312446174 40.0 38.0 41.0 33.0 41.0 16-17 38.05280856829108 40.0 38.0 41.0 33.0 41.0 18-19 38.001489097334726 40.0 38.0 41.0 33.0 41.0 20-21 37.88959349601169 40.0 38.0 41.0 33.0 41.0 22-23 37.73087992887442 40.0 38.0 41.0 32.0 41.0 24-25 37.671052240394246 40.0 38.0 41.0 32.0 41.0 26-27 37.67129857498741 40.0 38.0 41.0 32.0 41.0 28-29 37.57110103540753 40.0 37.5 41.0 32.0 41.0 30-31 37.36062275461535 40.0 37.0 41.0 31.5 41.0 32-33 37.11344486659 40.0 36.5 41.0 31.0 41.0 34-35 36.883255335243916 40.0 36.0 41.0 30.5 41.0 36-37 36.63942726735184 39.5 36.0 41.0 30.0 41.0 38-39 36.38463613030062 39.0 35.0 41.0 30.0 41.0 40-41 36.1354035663775 39.0 35.0 41.0 29.5 41.0 42-43 35.82526099905194 39.0 35.0 40.5 28.5 41.0 44-45 35.559495939018504 39.0 35.0 40.0 27.0 41.0 46-47 35.357876501425864 39.0 35.0 41.0 26.0 41.0 48-49 35.05613998411567 39.0 35.0 40.5 24.0 41.0 50-51 34.40029017460026 38.5 34.0 39.5 23.5 40.5 52-53 34.479234748844895 38.0 34.0 40.0 24.0 41.0 54-55 34.59017639350111 38.0 34.0 40.0 23.5 41.0 56-57 34.35728072092037 38.0 34.0 40.0 23.0 41.0 58-59 34.07518136502398 38.0 34.0 40.0 22.0 41.0 60-61 33.67792530587725 37.5 33.5 40.0 20.5 41.0 62-63 33.237573906276765 37.0 33.0 40.0 18.5 41.0 64-65 32.78463797073149 36.0 32.5 39.0 16.5 41.0 66-67 32.32466875783658 36.0 32.0 39.0 12.0 41.0 68-69 31.817254416916107 35.0 31.0 39.0 8.0 40.0 70-71 31.32266198033193 35.0 31.0 38.0 6.5 40.0 72-73 30.820120770017397 35.0 30.5 37.0 2.0 39.5 74-75 30.280712190117455 35.0 30.0 36.5 2.0 39.0 76-77 29.725470241884523 34.0 29.0 36.0 2.0 39.0 78-79 29.146972090101833 34.0 29.0 35.5 2.0 37.5 80-81 28.60108778902433 34.0 28.0 35.0 2.0 37.0 82-83 28.046129927812636 34.0 26.5 35.0 2.0 36.5 84-85 27.528881787237697 33.5 26.0 35.0 2.0 36.0 86-87 27.006697280739797 33.0 25.5 35.0 2.0 36.0 88-89 26.427395474144543 33.0 24.5 35.0 2.0 35.0 90-91 25.77739162811593 32.0 22.0 35.0 2.0 35.0 92-93 25.120034552909793 32.0 19.0 34.0 2.0 35.0 94-95 24.460945113160545 31.5 13.0 34.0 2.0 35.0 96-97 23.562868410608623 31.0 2.0 34.0 2.0 35.0 98-99 22.43464512009755 31.0 2.0 34.0 2.0 35.0 100-101 19.598942035393847 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5607692371697333 1101 2 0.4065645733650953 1101 3 0.3715283958041198 1101 4 0.2323896655496327 1101 5 0.35049404876319556 1101 6 0.29361489379984107 1101 7 0.4071883854105067 1101 8 0.36976273895562173 1101 9 0.4365158666572029 1101 10-11 0.3599397999198217 1101 12-13 0.31673729831920383 1101 14-15 0.4373048364902701 1101 16-17 0.5236137751797543 1101 18-19 0.600687403046166 1101 20-21 0.6218081747481605 1101 22-23 0.6563647825124121 1101 24-25 0.7670496474296797 1101 26-27 0.7387668736279025 1101 28-29 0.6280866588112346 1101 30-31 0.6360671301902769 1101 32-33 0.5866696823554918 1101 34-35 0.7037605969278431 1101 36-37 0.6868570353515722 1101 38-39 0.6992257943340547 1101 40-41 0.8204988192817311 1101 42-43 0.748196022331463 1101 44-45 0.6852681745136522 1101 46-47 0.6029950405695956 1101 48-49 0.6122699893832717 1101 50-51 0.49135362125272053 1101 52-53 0.7006697213911934 1101 54-55 0.7028479453229153 1101 56-57 0.6475462975945021 1101 58-59 0.7393716724885735 1101 60-61 0.7224473711324109 1101 62-63 0.8535145708153493 1101 64-65 0.7823780965551563 1101 66-67 0.678467923196564 1101 68-69 0.7802272315333205 1101 70-71 0.7315001925444236 1101 72-73 0.7503653490112789 1101 74-75 0.7958964404249365 1101 76-77 0.8279634674997247 1101 78-79 0.891341078049944 1101 80-81 1.0491488978434198 1101 82-83 0.8999220026137813 1101 84-85 1.0229717684984863 1101 86-87 1.213398887529447 1101 88-89 1.3111880152273727 1101 90-91 1.4795394884294915 1101 92-93 1.6350955842726087 1101 94-95 1.540440318173939 1101 96-97 1.6356672268903338 1101 98-99 1.7800055790299183 1101 100-101 1.6887768466722193 1102 1 0.4427884736124348 1102 2 0.31810330872175996 1102 3 0.25459059193649836 1102 4 0.25944345816935765 1102 5 0.2794966108705452 1102 6 0.3145228421499269 1102 7 0.19288994824336214 1102 8 0.23146489903179202 1102 9 0.403177999519464 1102 10-11 0.3414630695555161 1102 12-13 0.2606534914526222 1102 14-15 0.30579800766668797 1102 16-17 0.3022269518188949 1102 18-19 0.3355754988732471 1102 20-21 0.414098112555628 1102 22-23 0.35966214181603817 1102 24-25 0.4131419835735173 1102 26-27 0.39053399084021834 1102 28-29 0.5605880528868425 1102 30-31 0.4219936288180861 1102 32-33 0.4321322813777968 1102 34-35 0.49348662679167177 1102 36-37 0.5246123977295056 1102 38-39 0.41149911681026907 1102 40-41 0.38886171052665475 1102 42-43 0.49815550895706195 1102 44-45 0.3093860563830262 1102 46-47 0.3845636851846166 1102 48-49 0.252526599019113 1102 50-51 0.3767384456767573 1102 52-53 0.3045048766952263 1102 54-55 0.35690195595306307 1102 56-57 0.3192994588150242 1102 58-59 0.1319101200271504 1102 60-61 0.2539869984758525 1102 62-63 0.29662949943285355 1102 64-65 0.09429229740010925 1102 66-67 0.013270864758410994 1102 68-69 0.2395286729230044 1102 70-71 0.23049559936657005 1102 72-73 0.4578519989887404 1102 74-75 0.4721974876229531 1102 76-77 0.28624452588358196 1102 78-79 0.49468425555614104 1102 80-81 0.49879721405217836 1102 82-83 0.6533134221996484 1102 84-85 0.6541555983849783 1102 86-87 0.42205869402372187 1102 88-89 0.4881833993343321 1102 90-91 0.8261152703237649 1102 92-93 0.7536936747006777 1102 94-95 0.8283728174904077 1102 96-97 0.8774259754938356 1102 98-99 0.8219513955426976 1102 100-101 0.7672292994834855 1103 1 0.3693508596452837 1103 2 0.3513382849502875 1103 3 0.3225360335819971 1103 4 0.16505458733102074 1103 5 0.18838813735070659 1103 6 0.2034521642816003 1103 7 0.23210989904270463 1103 8 0.31015968919962233 1103 9 0.38352213887269215 1103 10-11 0.29296780064277783 1103 12-13 0.3364173283080234 1103 14-15 0.4023425568043564 1103 16-17 0.3646628484046843 1103 18-19 0.3066990906536873 1103 20-21 0.3204142300802104 1103 22-23 0.43338169808182414 1103 24-25 0.6092376264642851 1103 26-27 0.47060281871086573 1103 28-29 0.5365309217757144 1103 30-31 0.5461382867011295 1103 32-33 0.6739833399875792 1103 34-35 0.6135020102849111 1103 36-37 0.6203337973621572 1103 38-39 0.5356336958529155 1103 40-41 0.7749404448364885 1103 42-43 0.6267474551678802 1103 44-45 0.6941583873137915 1103 46-47 0.6294429048701744 1103 48-49 0.6538730525271461 1103 50-51 0.6752852360302413 1103 52-53 0.7563825248773881 1103 54-55 0.7033960445805576 1103 56-57 0.7122340590762732 1103 58-59 0.7269636638667905 1103 60-61 0.8177938825890791 1103 62-63 0.856331377943043 1103 64-65 0.9072251736491879 1103 66-67 0.8813275714973159 1103 68-69 1.0071116741629744 1103 70-71 1.1032556765492672 1103 72-73 1.1290637806227046 1103 74-75 0.865655391887227 1103 76-77 0.964506854228329 1103 78-79 1.0693916707977351 1103 80-81 1.092659598095782 1103 82-83 1.2535319738968091 1103 84-85 1.3619623233586182 1103 86-87 1.417473484722013 1103 88-89 1.414458671032019 1103 90-91 1.3913680701858446 1103 92-93 1.5563027682018138 1103 94-95 1.5042857150907132 1103 96-97 1.3881135248105316 1103 98-99 1.7368906241135562 1103 100-101 1.394214864196936 1104 1 0.2616724484023827 1104 2 0.21816151828029717 1104 3 0.06411283015331293 1104 4 0.13669965859584465 1104 5 0.12336944902219216 1104 6 0.16959289985421577 1104 7 0.20299722088063987 1104 8 0.19879020848597406 1104 9 0.07019586557457558 1104 10-11 0.2586202724856932 1104 12-13 0.13309167795419086 1104 14-15 0.2724272772269529 1104 16-17 0.2927254916818711 1104 18-19 0.39012839893634776 1104 20-21 0.3772322306782385 1104 22-23 0.3505453081685417 1104 24-25 0.436861218610197 1104 26-27 0.3771363829488834 1104 28-29 0.47916298596389595 1104 30-31 0.38054574209832026 1104 32-33 0.46248503439603184 1104 34-35 0.546082494686388 1104 36-37 0.5649676307121041 1104 38-39 0.4439189794171412 1104 40-41 0.6052120485442529 1104 42-43 0.5627969916973328 1104 44-45 0.6471061596650358 1104 46-47 0.6665763644535474 1104 48-49 0.662322793382323 1104 50-51 0.5892770630012407 1104 52-53 0.5613900002087959 1104 54-55 0.5893052940572829 1104 56-57 0.6205785223474862 1104 58-59 0.6916130708238271 1104 60-61 0.6001811296737003 1104 62-63 0.6476911407258612 1104 64-65 0.7858129589576706 1104 66-67 0.6345886848233349 1104 68-69 0.7322370105528826 1104 70-71 0.8978687035601354 1104 72-73 0.961842112128739 1104 74-75 0.8687113073661479 1104 76-77 0.8578889433342809 1104 78-79 0.9755569503751325 1104 80-81 0.9939320240900003 1104 82-83 0.9395002784916464 1104 84-85 0.9052885966567352 1104 86-87 0.808427087831749 1104 88-89 0.7915486491043779 1104 90-91 0.8624396589429466 1104 92-93 0.9475526436129584 1104 94-95 0.9214416006683095 1104 96-97 0.8953309572833703 1104 98-99 1.138068611754342 1104 100-101 0.808526635352127 1105 1 0.4417197223226914 1105 2 0.31216130949957943 1105 3 0.30532539875269293 1105 4 0.2971439141434473 1105 5 0.26952713811787277 1105 6 0.3028434665447435 1105 7 0.2514604456480001 1105 8 0.3870097563265844 1105 9 0.5237980786502163 1105 10-11 0.5150421437574622 1105 12-13 0.3474311852316916 1105 14-15 0.4741765614073472 1105 16-17 0.5046174178331526 1105 18-19 0.47312805593945484 1105 20-21 0.48899329605642805 1105 22-23 0.4766488634059556 1105 24-25 0.5377843276533909 1105 26-27 0.4510689915148589 1105 28-29 0.5173064779705783 1105 30-31 0.47736407304558526 1105 32-33 0.545525701897823 1105 34-35 0.6802632540516953 1105 36-37 0.6361618564338869 1105 38-39 0.6228179593741956 1105 40-41 0.5934905023738821 1105 42-43 0.8198936720838716 1105 44-45 0.7765315652961533 1105 46-47 0.7770195015330117 1105 48-49 0.7371837305094999 1105 50-51 0.541585581254644 1105 52-53 0.64123184881295 1105 54-55 0.7368386989725764 1105 56-57 0.8211776484510338 1105 58-59 0.6711544243194183 1105 60-61 0.8082460306519863 1105 62-63 0.7273391215180354 1105 64-65 0.8091870790960556 1105 66-67 0.9650624679299895 1105 68-69 1.0019556039673425 1105 70-71 0.9750206362730864 1105 72-73 1.079340919096051 1105 74-75 1.0247730318948989 1105 76-77 1.001291472563583 1105 78-79 1.1175576661947453 1105 80-81 1.1157965850539675 1105 82-83 1.17571515859186 1105 84-85 1.180379565399278 1105 86-87 1.272302430828649 1105 88-89 1.2382294084696852 1105 90-91 1.153282387408705 1105 92-93 1.162261245378648 1105 94-95 1.3833894502268862 1105 96-97 1.3913715800464317 1105 98-99 1.3620362656555578 1105 100-101 1.275509455681405 1106 1 0.059396534900820086 1106 2 0.0394549159050257 1106 3 0.06208552311149873 1106 4 0.118664372999433 1106 5 -0.03843266079586982 1106 6 0.1595095674542648 1106 7 0.19474261837095952 1106 8 0.07108759912153317 1106 9 0.1070297104015836 1106 10-11 -0.4805668399271781 1106 12-13 -0.32590400701797506 1106 14-15 -0.10100043975650408 1106 16-17 0.08583960144020608 1106 18-19 0.12488264396048976 1106 20-21 0.04293891332441291 1106 22-23 -0.059966400182993596 1106 24-25 -0.10979881170606376 1106 26-27 -0.048923263000425266 1106 28-29 -0.23167717973018398 1106 30-31 0.058509138565653984 1106 32-33 0.06218219840629047 1106 34-35 -0.02109678571294893 1106 36-37 -0.054938783679908454 1106 38-39 0.14185501377308896 1106 40-41 0.20246071660726273 1106 42-43 0.21515350278778556 1106 44-45 0.17065213622592523 1106 46-47 0.3083627841098462 1106 48-49 0.38302682988878445 1106 50-51 0.47948699941921546 1106 52-53 0.4764783168719191 1106 54-55 0.012879692118829666 1106 56-57 -0.004470480239859853 1106 58-59 0.250292735776803 1106 60-61 0.06483171322450687 1106 62-63 0.16268149888343686 1106 64-65 0.09084018106165814 1106 66-67 0.08909596614112303 1106 68-69 -0.2068175381939561 1106 70-71 0.016335725285546943 1106 72-73 0.3057380698353036 1106 74-75 0.36957001937280864 1106 76-77 0.17647413129887468 1106 78-79 0.41511494237044033 1106 80-81 0.46713379247670517 1106 82-83 0.2565386181159752 1106 84-85 0.13220534452768717 1106 86-87 0.4176895568901138 1106 88-89 0.22485834452296416 1106 90-91 0.2109727781583075 1106 92-93 0.31127224800806985 1106 94-95 0.14508614243344553 1106 96-97 0.44697338400349906 1106 98-99 0.3611987430252732 1106 100-101 0.2174088910018881 1107 1 0.3875368377836743 1107 2 0.2556642757869412 1107 3 0.20957041614304472 1107 4 0.171936590682904 1107 5 0.22497356179887618 1107 6 0.2318102554231345 1107 7 0.15466991551613063 1107 8 0.40482309931361726 1107 9 0.3513946070841243 1107 10-11 0.3136072899665052 1107 12-13 0.26473137590937057 1107 14-15 0.3089224329104425 1107 16-17 0.2689560067841157 1107 18-19 0.3523880288611707 1107 20-21 0.5058027570239929 1107 22-23 0.47610370326409424 1107 24-25 0.34950852283612477 1107 26-27 0.3042622649062494 1107 28-29 0.39112180304692146 1107 30-31 0.531946199666514 1107 32-33 0.4982318828327905 1107 34-35 0.49175605571267766 1107 36-37 0.5258525101467981 1107 38-39 0.48701980898189134 1107 40-41 0.3571391898815932 1107 42-43 0.5142902260213518 1107 44-45 0.3732737413494931 1107 46-47 0.2743984323344719 1107 48-49 0.2778686596130129 1107 50-51 0.27333084683406383 1107 52-53 0.4058025801320184 1107 54-55 0.4228281689091773 1107 56-57 0.2801296358464498 1107 58-59 0.3630247985885404 1107 60-61 0.4238418042487808 1107 62-63 0.4351498670780174 1107 64-65 0.2538104264991432 1107 66-67 0.2652957228698156 1107 68-69 0.34162396345466917 1107 70-71 0.3969989770692095 1107 72-73 0.25419696943221837 1107 74-75 0.3322197097557513 1107 76-77 0.4268099309842093 1107 78-79 0.34833371064283725 1107 80-81 0.4159943232812502 1107 82-83 0.2856527561558373 1107 84-85 0.3781567326889821 1107 86-87 0.564776246881177 1107 88-89 0.6060795305102431 1107 90-91 0.5885779977142 1107 92-93 0.6988353289618807 1107 94-95 0.7474878520604058 1107 96-97 0.7671533756344857 1107 98-99 0.5866031971142931 1107 100-101 0.6386579994106079 1108 1 0.20643075693151403 1108 2 0.09535475535220428 1108 3 0.06747986905897108 1108 4 0.06138019450741439 1108 5 0.1544525483867858 1108 6 0.14700348157439436 1108 7 0.1214120562581229 1108 8 0.07769467183555889 1108 9 0.2393961674470333 1108 10-11 0.13191102526692333 1108 12-13 0.036968566416184956 1108 14-15 0.11158871917807289 1108 16-17 0.1931792679022024 1108 18-19 0.18911648412208848 1108 20-21 0.2068686819529333 1108 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-2.0575304575842885 2316 94-95 -2.293294714871781 2316 96-97 -2.1790653254206553 2316 98-99 -2.2872075559932803 2316 100-101 -2.032789613972593 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7164.0 3 2168.0 4 2265.0 5 2402.0 6 2801.0 7 3317.0 8 4250.0 9 5072.0 10 6246.0 11 7801.0 12 9350.0 13 11365.0 14 13217.0 15 15842.0 16 19160.0 17 25614.0 18 23027.0 19 18206.0 20 17908.0 21 18270.0 22 19472.0 23 21138.0 24 23695.0 25 26003.0 26 29281.0 27 34187.0 28 39244.0 29 46169.0 30 54905.0 31 66714.0 32 83055.0 33 107743.0 34 145240.0 35 211780.0 36 305592.0 37 392882.0 38 268288.0 39 28235.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.92825654857238 18.461968221206128 15.081718727842796 22.52805650237869 2 15.609124113783244 19.670376469788366 40.522910379264886 24.197589037163503 3 19.622652022137725 24.050621555146225 29.50761462306046 26.819111799655595 4 12.933269803701938 16.641442128928947 36.54867879301886 33.87660927435026 5 13.889439888465091 37.07383331349735 33.72235477697741 15.31437202106015 6 32.0696101260369 35.69651935197219 17.239357679019975 14.994512842970936 7 28.603129238336667 32.960658790994984 19.936946773102832 18.499265197565517 8 26.683368304157405 34.503360787476225 19.159510395872246 19.653760512494124 9 26.539296877213193 16.92905504301268 19.993673216933118 36.53797486284101 10-11 25.264065383750783 26.581724021331137 26.345663783348794 21.80854681156929 12-13 26.380238253613108 23.93491677752918 27.034098725585054 22.650746243272664 14-15 23.146060306587565 25.148740840701812 25.853602796972325 25.8515960557383 16-17 22.179947869939276 28.422735128294725 26.56432679504009 22.83299020672591 18-19 22.138694451579756 26.55256040253245 29.389312075973468 21.919433069914326 20-21 22.876309935914087 25.526500965775195 29.85012774679216 21.747061351518557 22-23 22.651496710219536 25.350894569542493 29.7990338077565 22.198574912481476 24-25 22.375783768465055 25.60759030311618 29.814705917082907 22.20192001133586 26-27 21.858699440226438 25.961210629558728 29.793255895000193 22.38683403521464 28-29 21.94412523525527 26.354988899233184 29.64474948958667 22.056136375924876 30-31 22.087811567764174 25.91958563571134 29.543573089177766 22.44902970734672 32-33 21.8906938695904 25.9159511637533 29.55187003572261 22.641484930933693 34-35 22.545560587671368 26.060750598580405 29.320522689385175 22.07316612436305 36-37 22.612319722592204 25.608393824248104 29.387003097131686 22.392283356028003 38-39 22.117331137733597 26.104369986252323 29.240086753886363 22.538212122127717 40-41 22.571779963693963 25.926028218619944 29.280229261533304 22.22196255615279 42-43 22.210886385090454 25.92014435173555 29.139566685133957 22.72940257804004 44-45 22.31119315663547 26.158556070097 29.108513282548394 22.42173749071914 46-47 22.67603506304078 26.466530090112204 28.467667703578524 22.38976714326849 48-49 22.413749968727327 26.49878709234006 28.442436804419053 22.64502613451356 50-51 22.51070092725635 26.783826164451586 28.268149093607388 22.437323814684675 52-53 22.80333157895979 26.5859340151002 28.113749489618904 22.496984916321104 54-55 22.508701854224235 26.78410432973301 28.180908882358107 22.52628493368465 56-57 22.477312683160655 26.9153674754116 28.102322861211043 22.504996980216703 58-59 22.586372466163393 26.519204540922182 28.04731236493147 22.847110627982953 60-61 22.33142202213798 26.77388702352343 28.28445540848199 22.6102355458566 62-63 22.30003648551506 26.70130823935442 28.49589526155583 22.502760013574687 64-65 22.16171821622138 26.80907253282545 28.34799519020293 22.68121406075024 66-67 22.176413533674893 26.781398158789642 28.11147322787633 22.930715079659134 68-69 22.440155683015334 26.5294544108929 28.276640373367368 22.753749532724395 70-71 22.401614030565455 26.605798244763008 27.979362427711962 23.01322529695958 72-73 22.38757035261994 26.637430379113404 27.948956170589227 23.02604309767743 74-75 22.261044228842394 27.136802923963494 27.80024146065997 22.801911386534144 76-77 22.42422139808258 26.78371243284985 27.764584935237067 23.027481233830507 78-79 22.518762233032575 26.925462218070926 27.756753861119122 22.799021687777373 80-81 22.587291042513048 26.96532429635591 27.54150240536366 22.905882255767384 82-83 22.467091661899392 27.04322452624643 27.663772318857816 22.825911492996358 84-85 22.608899224469585 26.996940074452052 27.554315684442994 22.839845016635365 86-87 22.583110729354463 27.019482174376787 27.399379855205158 22.99802724106359 88-89 22.60650671843476 27.088972551470864 27.181340259455773 23.123180470638612 90-91 22.541042722770722 26.99743690288537 27.255446374800485 23.206073999543424 92-93 22.690627308711793 26.673967998451616 27.40251187843283 23.232892814403765 94-95 22.558540378206242 26.609050348254666 27.469565047002515 23.362844226536573 96-97 22.672140373141165 26.39736729543002 27.48346887767772 23.447023453751097 98-99 22.538040704840576 26.468980415168037 27.18924356672708 23.803735313264312 100-101 22.920222836221996 25.91466138490301 27.377939970360988 23.787175808514007 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1577.0 1 1064.0 2 702.5 3 907.0 4 737.0 5 626.5 6 1200.0 7 2797.5 8 4715.5 9 5442.0 10 4376.5 11 2988.5 12 2728.0 13 2852.0 14 2988.5 15 3113.5 16 3247.5 17 3436.0 18 3631.0 19 3912.5 20 4243.5 21 4892.0 22 5715.5 23 6776.5 24 8217.5 25 10134.0 26 12760.0 27 15672.5 28 17982.0 29 20410.0 30 23305.0 31 27367.5 32 32707.5 33 35666.5 34 37060.5 35 39372.5 36 43179.0 37 47117.5 38 50423.0 39 53128.0 40 55928.5 41 59848.0 42 62140.0 43 64184.0 44 67012.0 45 70130.0 46 71813.5 47 74293.0 48 77633.0 49 77583.5 50 76301.0 51 74715.5 52 73523.0 53 74382.5 54 76352.5 55 73303.5 56 67480.0 57 62974.5 58 59178.0 59 53456.0 60 43876.5 61 35998.0 62 29848.5 63 22653.5 64 16702.5 65 13016.5 66 9782.5 67 7358.5 68 5578.0 69 4086.0 70 3024.0 71 2012.5 72 1388.0 73 1000.5 74 682.0 75 486.0 76 329.0 77 194.0 78 125.0 79 87.5 80 58.5 81 41.0 82 25.0 83 14.5 84 13.0 85 13.0 86 12.0 87 8.0 88 5.0 89 5.0 90 7.0 91 6.5 92 3.5 93 3.5 94 3.5 95 2.0 96 0.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.49814328839693284 2 0.05082420629059271 3 0.05832750136970528 4 0.06710494089621433 5 0.08045986232633293 6 0.1089157549848542 7 0.07045546888751616 8 0.05252306555378801 9 0.05139049271165781 10-11 0.07555204667710207 12-13 0.11009551836207315 14-15 0.05698257111967567 16-17 0.060828599729409474 18-19 0.060262313308344376 20-21 0.07545766560692456 22-23 0.06486339047949831 24-25 0.08937887345810826 26-27 0.06734089357165812 28-29 0.0671285361637587 30-31 0.07135208905420258 32-33 0.07090377897085937 34-35 0.07357004420337421 36-37 0.06911053863748656 38-39 0.04214114783426118 40-41 0.045491675825563016 42-43 0.04291979166322569 44-45 0.021802027211006342 46-47 0.028267130518166234 48-49 0.02859746426378754 50-51 0.026473890184793417 52-53 0.02614355643917211 54-55 0.026190746974260867 56-57 0.02545929368038511 58-59 0.022108765689083272 60-61 0.029635656035740227 62-63 0.019820024737278494 64-65 0.02248628996979334 66-67 0.016705449421420444 68-69 0.01911216671094712 70-71 0.04216474310180556 72-73 0.038271523956982995 74-75 0.03357606571565155 76-77 0.023406505404024128 78-79 0.015006590158225147 80-81 0.017295331110029923 82-83 0.02156607453556255 84-85 0.018404308684615744 86-87 0.009862821833550488 88-89 0.0426838389877819 90-91 0.05301856617221997 92-93 0.03463785275514861 94-95 0.05429271061961644 96-97 0.04450067458869909 98-99 0.035204139176213706 100-101 0.17054659381077256 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2119069.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.82302506705197 #Duplication Level Percentage of deduplicated Percentage of total 1 78.93815394965632 44.85504700628128 2 12.120654616954726 13.774645222565951 3 3.681213482084505 6.275330579089725 4 1.6686453866355304 3.7926991453124552 5 0.9120169208623004 2.591178017786702 6 0.5511564403315499 1.8791025734896074 7 0.37463849109428254 1.4901664659373062 8 0.2773062255539187 1.2605902884719913 9 0.2071660063638115 1.059461925538672 >10 1.142242425227838 11.787698953453523 >50 0.08137240417836389 3.175229719707326 >100 0.040934017002783984 4.351496580644279 >500 0.002833102922940667 1.1718322367983915 >1k 0.0014165514614703266 1.147068882325659 >5k 8.332655655707803E-5 0.3077190071094881 >10k+ 1.6665311311415607E-4 1.0807333954876235 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12328 0.5817649165742125 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10497 0.49535904682669607 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6499 0.3066912875418403 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2680 0.12647063403787231 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 9.438107017751663E-5 7 4.7190535088758317E-5 0.0 0.0 0.0 9.438107017751663E-5 8 4.7190535088758317E-5 0.0 0.0 0.0 9.438107017751663E-5 9 4.7190535088758317E-5 0.0 0.0 0.0 9.438107017751663E-5 10-11 4.7190535088758317E-5 0.0 0.0 4.7190535088758317E-5 9.438107017751663E-5 12-13 4.7190535088758317E-5 0.0 0.0 4.7190535088758317E-5 1.8876214035503327E-4 14-15 4.7190535088758317E-5 0.0 0.0 4.7190535088758317E-5 1.8876214035503327E-4 16-17 4.7190535088758317E-5 0.0 0.0 9.438107017751663E-5 1.8876214035503327E-4 18-19 4.7190535088758317E-5 0.0 0.0 9.438107017751663E-5 2.359526754437916E-4 20-21 4.7190535088758317E-5 0.0 0.0 2.1235740789941242E-4 2.359526754437916E-4 22-23 9.438107017751663E-5 0.0 0.0 2.831432105325499E-4 2.831432105325499E-4 24-25 9.438107017751663E-5 0.0 0.0 6.134769561538581E-4 2.831432105325499E-4 26-27 9.438107017751663E-5 0.0 0.0 0.0011797633772189578 2.831432105325499E-4 28-29 1.4157160526627496E-4 0.0 0.0 0.002689860500059224 2.831432105325499E-4 30-31 1.4157160526627496E-4 0.0 0.0 0.008871820596686564 3.303337456213082E-4 32-33 1.4157160526627496E-4 0.0 0.0 0.02359526754437916 3.7752428071006653E-4 34-35 1.4157160526627496E-4 0.0 0.0 0.04407595977290027 3.7752428071006653E-4 36-37 2.359526754437916E-4 0.0 0.0 0.07819471664207253 3.7752428071006653E-4 38-39 2.359526754437916E-4 0.0 0.0 0.1282874696387895 3.7752428071006653E-4 40-41 2.359526754437916E-4 0.0 0.0 0.18175434589435266 3.7752428071006653E-4 42-43 2.595479429881708E-4 0.0 0.0 0.23543357955781524 4.0111954825444566E-4 44-45 2.831432105325499E-4 0.0 0.0 0.30397783177423665 4.955006184319624E-4 46-47 2.831432105325499E-4 0.0 0.0 0.38467364677601346 5.662864210650998E-4 48-49 2.831432105325499E-4 0.0 0.0 0.4680121317427606 6.134769561538581E-4 50-51 2.831432105325499E-4 0.0 0.0 0.5550786689815197 6.134769561538581E-4 52-53 3.0673847807692904E-4 0.0 0.0 0.6378508675272018 6.606674912426164E-4 54-55 3.303337456213082E-4 0.0 0.0 0.7293061245292154 7.078580263313748E-4 56-57 3.7752428071006653E-4 0.0 0.0 0.8354848284789216 7.31453293875754E-4 58-59 3.7752428071006653E-4 0.0 0.0 0.949143232240196 7.550485614201331E-4 60-61 3.7752428071006653E-4 0.0 0.0 1.0714375039227133 7.550485614201331E-4 62-63 3.7752428071006653E-4 0.0 0.0 1.1927643696359107 8.022390965088914E-4 64-65 3.7752428071006653E-4 0.0 0.0 1.3167575005816232 8.022390965088914E-4 66-67 3.7752428071006653E-4 0.0 0.0 1.4652661145059458 8.022390965088914E-4 68-69 3.7752428071006653E-4 0.0 0.0 1.6229532875050316 8.022390965088914E-4 70-71 4.2471481579882484E-4 0.0 0.0 1.7911875450964552 8.494296315976497E-4 72-73 4.719053508875832E-4 0.0 0.0 1.9596813506308668 8.96620166686408E-4 74-75 4.719053508875832E-4 0.0 0.0 2.1297796343582958 9.438107017751664E-4 76-77 4.719053508875832E-4 0.0 0.0 2.3132328395158437 9.438107017751664E-4 78-79 4.719053508875832E-4 0.0 0.0 2.525495866345079 9.438107017751664E-4 80-81 7.078580263313748E-4 0.0 0.0 2.741203802235793 0.0010381917719526831 82-83 7.550485614201331E-4 0.0 0.0 2.9641318899950875 0.0012741444473964745 84-85 7.550485614201331E-4 0.0 0.0 3.1904105057456835 0.0012741444473964745 86-87 7.550485614201331E-4 0.0 0.0 3.4243339881806585 0.001344930250029612 88-89 7.550485614201331E-4 0.0 0.0 3.6848965276732377 0.0014157160526627496 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6655 0.0 46.11544 1 GTATCAA 10760 0.0 37.349365 1 TATCAAC 13460 0.0 29.620188 2 ATCAACG 13635 0.0 29.065767 3 TCAACGC 13840 0.0 28.600906 4 CAACGCA 13920 0.0 28.504808 5 AACGCAG 14385 0.0 27.616415 6 ACGCAGA 16010 0.0 24.706326 7 CGCAGAG 16145 0.0 24.440844 8 GAGTACT 9330 0.0 23.588959 12-13 TAAGGTG 1070 0.0 23.537645 5 GTACTTT 9805 0.0 22.21334 14-15 GCAGAGT 17405 0.0 22.206093 9 TACATGG 9470 0.0 22.128883 2 GTACATG 9655 0.0 21.751228 1 AGAGTAC 14670 0.0 20.788698 10-11 AGTACTT 9715 0.0 20.770374 12-13 ACATGGG 9670 0.0 20.688383 3 GTACCTG 1875 0.0 20.618979 1 TGGTATC 3545 0.0 20.509075 2 >>END_MODULE