FastQCFastQC Report
Thu 26 May 2016
SRR938145_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938145_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2119069
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120160.5670414696265199No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105480.49776576411622275No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65660.3098530533927871No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27440.12949082828355282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66800.045.514431
GTATCAA106800.035.4958231
TATCAAC131500.028.7562942
ATCAACG132450.028.4052333
TCAACGC134700.027.9268044
CAACGCA136000.027.7297025
AACGCAG140050.026.9278096
CGCAGAG156650.023.9227038
ACGCAGA157300.023.8842377
GAGTACT94600.023.42200912-13
GTACTTT97150.022.5383314-15
GTGTAAG9250.022.0837251
GCAGAGT171600.021.3956669
TGGTATC37200.021.3264792
TACATGG88050.021.2035622
GTACATG90200.021.0668241
GTACCTG17700.020.3979231
AGTACTT98600.020.35229512-13
CAGAGTA164050.020.17995510-11
GTGGTAT40100.020.1395681
Produced by FastQC (version 0.11.5)