##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938145_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2119069 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49496830919616 33.0 31.0 34.0 31.0 34.0 2 32.708433278954104 34.0 31.0 34.0 31.0 34.0 3 32.76606283230985 34.0 31.0 34.0 31.0 34.0 4 36.23059371827911 37.0 35.0 37.0 35.0 37.0 5 36.196407950850116 37.0 35.0 37.0 35.0 37.0 6 36.23638777217731 37.0 37.0 37.0 35.0 37.0 7 36.202857481280695 37.0 37.0 37.0 35.0 37.0 8 36.19480016931964 37.0 37.0 37.0 35.0 37.0 9 37.90108297558975 39.0 38.0 39.0 35.0 39.0 10-11 37.88666555926211 39.0 38.0 39.0 35.0 39.0 12-13 37.83353066842089 39.0 38.0 39.0 35.0 39.0 14-15 39.13310680303473 40.5 38.5 41.0 36.0 41.0 16-17 39.08360251601057 40.0 38.0 41.0 36.0 41.0 18-19 39.11970091582671 40.0 38.5 41.0 36.0 41.0 20-21 39.05216724891922 40.0 39.0 41.0 35.5 41.0 22-23 38.91253918584057 40.0 38.0 41.0 35.0 41.0 24-25 38.87463928734742 40.0 38.0 41.0 35.0 41.0 26-27 38.83273951910013 40.0 38.0 41.0 35.0 41.0 28-29 38.77274218064632 40.0 38.0 41.0 35.0 41.0 30-31 38.56634470137594 40.0 38.0 41.0 34.5 41.0 32-33 38.379939492295904 40.0 38.0 41.0 34.0 41.0 34-35 38.175733541475054 40.0 38.0 41.0 34.0 41.0 36-37 38.0402603690583 40.0 37.5 41.0 33.5 41.0 38-39 38.03177173560653 40.0 38.0 41.0 33.0 41.0 40-41 37.969424308505296 40.0 38.0 41.0 33.0 41.0 42-43 37.80611627087178 40.0 37.0 41.0 33.0 41.0 44-45 37.58075834246077 40.0 37.0 41.0 33.0 41.0 46-47 37.229434482784654 40.0 37.0 41.0 32.0 41.0 48-49 36.858825267133824 40.0 36.5 41.0 31.0 41.0 50-51 36.54459458375352 40.0 36.0 41.0 30.5 41.0 52-53 36.12515920906775 39.0 35.0 41.0 29.5 41.0 54-55 35.89912527624159 39.0 35.0 41.0 29.0 41.0 56-57 35.64857916377428 39.0 35.0 40.0 28.0 41.0 58-59 35.31162033893186 38.0 35.0 40.0 28.0 41.0 60-61 34.950063447674424 38.0 34.0 40.0 27.0 41.0 62-63 34.58103393518569 37.0 34.0 40.0 26.0 41.0 64-65 34.18704393297245 37.0 34.0 39.5 26.0 41.0 66-67 33.7648967541878 36.0 33.5 39.0 25.5 41.0 68-69 33.30282732652877 35.5 33.0 39.0 24.5 40.0 70-71 32.79602528280108 35.0 32.5 38.5 23.5 40.0 72-73 32.382774935596714 35.0 32.0 37.5 22.0 39.5 74-75 31.934820668888083 35.0 32.0 37.0 21.5 39.0 76-77 30.652279609583267 33.5 30.0 35.5 20.0 38.0 78-79 31.37386890186209 34.5 31.5 36.0 20.5 38.5 80-81 31.215264108908205 35.0 31.5 36.0 20.0 37.0 82-83 30.85543391932967 34.5 31.0 35.0 18.5 37.0 84-85 30.481942069842937 34.0 31.0 35.0 17.0 36.5 86-87 30.05846223034738 34.0 30.5 35.0 9.5 36.0 88-89 29.59907440484477 34.0 30.0 35.0 7.0 36.0 90-91 29.256279054622574 34.0 30.0 35.0 2.0 35.0 92-93 28.8511433558794 34.0 29.0 35.0 2.0 35.0 94-95 28.440411095627372 34.0 29.0 35.0 2.0 35.0 96-97 27.860487553732323 34.0 28.5 35.0 2.0 35.0 98-99 27.1791749112464 33.0 27.0 35.0 2.0 35.0 100-101 24.762318263350558 31.0 21.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10813795627458234 1101 2 0.08313439782228471 1101 3 0.15708052286030494 1101 4 0.08238846448810477 1101 5 0.021946031032236135 1101 6 0.024120962872665075 1101 7 0.11980633857744039 1101 8 0.05778558580458082 1101 9 0.05170703842731683 1101 10-11 0.11222016840020643 1101 12-13 0.1973349043507895 1101 14-15 0.1894082215417754 1101 16-17 0.17437230249303326 1101 18-19 0.316070593633917 1101 20-21 0.30766387055087563 1101 22-23 0.2993818816064575 1101 24-25 0.19397352781064114 1101 26-27 0.24225566060193415 1101 28-29 0.3415376080827386 1101 30-31 0.323522332345739 1101 32-33 0.2277189259723542 1101 34-35 0.08914416123187152 1101 36-37 0.11274555848277856 1101 38-39 0.28696856740621257 1101 40-41 0.3787671800061858 1101 42-43 0.37410862998269323 1101 44-45 0.34024742868782454 1101 46-47 0.30678533356397253 1101 48-49 0.40620656472803063 1101 50-51 0.3560594559961956 1101 52-53 0.30867557332403095 1101 54-55 0.346952839278579 1101 56-57 0.36868379563961895 1101 58-59 0.32924049570789293 1101 60-61 0.3656696749602588 1101 62-63 0.4039517891947142 1101 64-65 0.5500475134544516 1101 66-67 0.4933919012556842 1101 68-69 0.5434111833514095 1101 70-71 0.6323243604790321 1101 72-73 0.5005765118224659 1101 74-75 0.5883851642780975 1101 76-77 0.31865675483014044 1101 78-79 0.4530628822589833 1101 80-81 0.5475384166722534 1101 82-83 0.5767535807706636 1101 84-85 0.7658381559882912 1101 86-87 0.7095628331985218 1101 88-89 0.5911611508166388 1101 90-91 0.6411389260129532 1101 92-93 0.8472422592593389 1101 94-95 0.73224359276265 1101 96-97 0.8669165129105849 1101 98-99 0.9725413481457572 1101 100-101 1.0032002997878813 1102 1 0.11645753352825494 1102 2 0.1410495477189997 1102 3 0.07496289085803909 1102 4 0.03900660467351713 1102 5 0.03504300079804068 1102 6 0.029959321018651508 1102 7 0.0999222359147467 1102 8 0.13857216301033048 1102 9 0.09289522021200014 1102 10-11 0.15481295233801973 1102 12-13 0.14189848043101705 1102 14-15 0.18985144478307348 1102 16-17 0.20788912101444623 1102 18-19 0.24340206561431188 1102 20-21 0.21695006229091263 1102 22-23 0.2822636304767272 1102 24-25 0.14650708881297447 1102 26-27 0.12250754436995948 1102 28-29 0.17171108288923875 1102 30-31 0.16165955924751074 1102 32-33 0.09937768064247621 1102 34-35 0.1574461092811248 1102 36-37 0.12944427111317935 1102 38-39 0.13768488541778368 1102 40-41 0.1681501273797963 1102 42-43 0.030636380328331825 1102 44-45 0.17618474473286483 1102 46-47 0.18805732332329939 1102 48-49 0.07860697038810116 1102 50-51 0.14440761560863535 1102 52-53 0.2114143882392696 1102 54-55 0.26335775030290876 1102 56-57 0.33086078631448146 1102 58-59 0.17208613850485932 1102 60-61 0.08595134887039535 1102 62-63 0.12034293259710438 1102 64-65 0.16382319650994503 1102 66-67 0.10608764318230612 1102 68-69 0.4392099533515079 1102 70-71 0.3321745510234777 1102 72-73 0.19121943206527448 1102 74-75 0.264643274224607 1102 76-77 0.15456193719018785 1102 78-79 0.3247925526477111 1102 80-81 0.31735707465321994 1102 82-83 0.3742789546086911 1102 84-85 0.3716191998288849 1102 86-87 0.23895892214124004 1102 88-89 0.16328174410418939 1102 90-91 0.17503068369505215 1102 92-93 0.39369510210705627 1102 94-95 0.34132194050397047 1102 96-97 0.5182211543720641 1102 98-99 0.5987089989988661 1102 100-101 0.9483364648929715 1103 1 0.14566521731789805 1103 2 0.1887614520230585 1103 3 0.15848544557884736 1103 4 0.059025834040077996 1103 5 0.11325904604672843 1103 6 0.07948568182787596 1103 7 0.0935746883220645 1103 8 0.024216313635946562 1103 9 0.07800161165560127 1103 10-11 0.0958209689946301 1103 12-13 0.09411231098714978 1103 14-15 0.18474080961720318 1103 16-17 0.1622660563838778 1103 18-19 0.20575176159139374 1103 20-21 0.17696053898027486 1103 22-23 0.1662169230084416 1103 24-25 0.21471816094963003 1103 26-27 0.1670846640760857 1103 28-29 0.20722812077109154 1103 30-31 0.16935448866247071 1103 32-33 0.12560109935861874 1103 34-35 0.21352185972372695 1103 36-37 0.31448223253509155 1103 38-39 0.2799211944403339 1103 40-41 0.343754294184599 1103 42-43 0.27957034403125647 1103 44-45 0.4254391094675327 1103 46-47 0.3288739746285714 1103 48-49 0.4841092215501561 1103 50-51 0.47578821781934977 1103 52-53 0.5643882429315639 1103 54-55 0.5235499113521627 1103 56-57 0.4357120239562988 1103 58-59 0.3855092972930194 1103 60-61 0.34680858076992394 1103 62-63 0.49184540920689557 1103 64-65 0.3873080881955673 1103 66-67 0.3260343314212335 1103 68-69 0.4326293988826677 1103 70-71 0.43834903431713457 1103 72-73 0.2900486951826835 1103 74-75 0.20141035354906123 1103 76-77 0.28741656980982455 1103 78-79 0.22485143429427623 1103 80-81 0.2694242315582187 1103 82-83 0.4006629716896697 1103 84-85 0.3472903378758474 1103 86-87 0.532540026967812 1103 88-89 0.4563079822433167 1103 90-91 0.3444791987237963 1103 92-93 0.44791714161346263 1103 94-95 0.3802989382030795 1103 96-97 0.5042612973324623 1103 98-99 0.6418167298673048 1103 100-101 0.8904085949100136 1104 1 0.04411707618364602 1104 2 0.027960594851727194 1104 3 0.07277603662838317 1104 4 0.010755376549795415 1104 5 0.02621927728364426 1104 6 0.04428459541304619 1104 7 0.059433853078374455 1104 8 0.09448293378944328 1104 9 -9.89218604544817E-4 1104 10-11 -0.02187986824248611 1104 12-13 -0.003446121186676976 1104 14-15 0.14230908431071754 1104 16-17 0.12919755743042316 1104 18-19 0.07767342011825917 1104 20-21 0.0708188066968134 1104 22-23 0.11471973871659458 1104 24-25 0.07136597316929993 1104 26-27 0.024722833118239862 1104 28-29 0.032372145489517834 1104 30-31 0.030353566704931723 1104 32-33 0.04705442837287421 1104 34-35 0.18508868918650023 1104 36-37 0.18510928825121908 1104 38-39 0.09904371512208598 1104 40-41 0.11722733315600209 1104 42-43 0.16549965231391184 1104 44-45 0.18266900082605275 1104 46-47 0.22704999376542645 1104 48-49 0.18391174821217504 1104 50-51 0.1717551519367717 1104 52-53 0.057726975115365065 1104 54-55 0.1725842231233159 1104 56-57 0.18170923316590404 1104 58-59 0.2532184819835308 1104 60-61 0.2816254351504668 1104 62-63 0.13047430883811728 1104 64-65 0.20894643166212745 1104 66-67 0.09131677435953378 1104 68-69 0.16166520572010512 1104 70-71 0.07869564051667055 1104 72-73 0.0595130876874137 1104 74-75 -0.10576621377875384 1104 76-77 0.06446717780344358 1104 78-79 0.21271685341119095 1104 80-81 0.1747890277308528 1104 82-83 0.2924170582041725 1104 84-85 0.3617200946783967 1104 86-87 0.21587888374046216 1104 88-89 0.3279045884428555 1104 90-91 0.3317860859957982 1104 92-93 0.5532698526669826 1104 94-95 0.5181553869223059 1104 96-97 0.5736099467892544 1104 98-99 0.48944916782205183 1104 100-101 0.6375877786038231 1105 1 0.11831625299108595 1105 2 0.1289247143936052 1105 3 0.09656585409437923 1105 4 0.06657066526370414 1105 5 0.07730344573986514 1105 6 0.09107011796136533 1105 7 0.09226834961857833 1105 8 0.15642742794358355 1105 9 0.026003145986244647 1105 10-11 0.07986345077446089 1105 12-13 0.15582560692117653 1105 14-15 0.18606728641044867 1105 16-17 0.19279552032710967 1105 18-19 0.12692516492856498 1105 20-21 0.19335258443482672 1105 22-23 0.11505155093325214 1105 24-25 0.11570205047782167 1105 26-27 0.1716429446668002 1105 28-29 0.19073418617193738 1105 30-31 0.29107221977103137 1105 32-33 0.30833604373913204 1105 34-35 0.3264742475257947 1105 36-37 0.36519198032949163 1105 38-39 0.24343049631988833 1105 40-41 0.29489413403884157 1105 42-43 0.3751565062867144 1105 44-45 0.44506535611118636 1105 46-47 0.29677529798875213 1105 48-49 0.3157008498585583 1105 50-51 0.3448119999444046 1105 52-53 0.4244577672890628 1105 54-55 0.44003605442849647 1105 56-57 0.4585114581718699 1105 58-59 0.46912384466546797 1105 60-61 0.38977571253056453 1105 62-63 0.42761401296601065 1105 64-65 0.5128976067993207 1105 66-67 0.3705908139682137 1105 68-69 0.356638437049142 1105 70-71 0.5167045435231117 1105 72-73 0.4525875329272182 1105 74-75 0.3866505721911828 1105 76-77 0.3540945042171906 1105 78-79 0.28559810191989854 1105 80-81 0.384423512340728 1105 82-83 0.5378537081683845 1105 84-85 0.5948274974462322 1105 86-87 0.6688006953605985 1105 88-89 0.6179494762977846 1105 90-91 0.4783669288733954 1105 92-93 0.5946102994577238 1105 94-95 0.5666279322575463 1105 96-97 0.670085280838407 1105 98-99 0.678211172713489 1105 100-101 0.6265839161604951 1106 1 -0.10459340906331249 1106 2 -0.057635724633939844 1106 3 -0.021209292463659324 1106 4 0.006315508791445268 1106 5 -0.01760770344760232 1106 6 -0.08388960551809532 1106 7 0.010670678382744825 1106 8 -0.03983035795236134 1106 9 -0.10732156628583311 1106 10-11 -0.11125335382919133 1106 12-13 -0.1284259367876075 1106 14-15 -0.3103691170983254 1106 16-17 -0.24277046581453732 1106 18-19 -0.14841705079194156 1106 20-21 -0.02581802906144759 1106 22-23 -0.0807078298590227 1106 24-25 -0.12207220703328403 1106 26-27 -0.15075881564614235 1106 28-29 -0.0018148595843499038 1106 30-31 -0.0253564247826219 1106 32-33 -0.024447696939347452 1106 34-35 -0.028877141542693607 1106 36-37 -0.11713248678116628 1106 38-39 -0.0036693247930870143 1106 40-41 0.06793493051713284 1106 42-43 -0.0747638836007809 1106 44-45 0.08976919571634312 1106 46-47 0.1382461049200927 1106 48-49 0.012773595451037068 1106 50-51 0.11688730491444943 1106 52-53 0.05925748925984209 1106 54-55 -0.21542794725260705 1106 56-57 0.012551368297309295 1106 58-59 -0.0047954244840582305 1106 60-61 0.05627770345689953 1106 62-63 0.08249272541051056 1106 64-65 -0.07568042996950908 1106 66-67 -0.012042866362833138 1106 68-69 -0.26920649430565646 1106 70-71 -0.4218599554854663 1106 72-73 -0.25883187092639304 1106 74-75 -0.28354130030332314 1106 76-77 -0.1470201720619606 1106 78-79 -0.14206479572655084 1106 80-81 -0.3021988905645685 1106 82-83 -0.31928048942634035 1106 84-85 -0.29477375153001617 1106 86-87 -0.0584279163252468 1106 88-89 -0.13064353956496433 1106 90-91 -0.2573790219218992 1106 92-93 0.05571146491744727 1106 94-95 0.09585097258256425 1106 96-97 0.09549356891116645 1106 98-99 0.10596903673779678 1106 100-101 -0.006974170933780499 1107 1 0.12807136739769476 1107 2 0.10648949174232314 1107 3 0.03242459685410637 1107 4 0.03989354898113362 1107 5 0.09401213338449566 1107 6 0.08383947157431493 1107 7 0.07841661777232645 1107 8 0.1126971546959652 1107 9 -0.020937110624139166 1107 10-11 0.043803717654732566 1107 12-13 0.05055931288044491 1107 14-15 0.10983922118978029 1107 16-17 0.08095264369590893 1107 18-19 0.11063807777825474 1107 20-21 0.020783403869572226 1107 22-23 0.08101177735015597 1107 24-25 0.07474058311553478 1107 26-27 0.0777246681944419 1107 28-29 0.08765837467923632 1107 30-31 0.11606832577000858 1107 32-33 0.18669601032775063 1107 34-35 0.2434864532933716 1107 36-37 0.12388341198677466 1107 38-39 0.23454147620459054 1107 40-41 0.19802005405006895 1107 42-43 0.21595968892648898 1107 44-45 0.15696170037168145 1107 46-47 0.21116675549193786 1107 48-49 0.03911273931343118 1107 50-51 0.11029044001615063 1107 52-53 0.059861596589755095 1107 54-55 0.10975009782221434 1107 56-57 0.12759005194968154 1107 58-59 -0.007292844252624775 1107 60-61 -0.036029767997717954 1107 62-63 0.009771015663567084 1107 64-65 -0.010687415931371902 1107 66-67 -0.040477477395491235 1107 68-69 -5.534618882592213E-4 1107 70-71 0.06079030483027026 1107 72-73 -0.0029425475887450148 1107 74-75 -0.0012240221480546154 1107 76-77 -0.09123821463422388 1107 78-79 -0.0760457955386471 1107 80-81 0.21342658817361837 1107 82-83 0.1626162168977885 1107 84-85 0.19982409982337046 1107 86-87 0.1877401962780425 1107 88-89 0.27581206342070885 1107 90-91 0.27622944325124266 1107 92-93 0.3584003049692335 1107 94-95 0.33710984269723454 1107 96-97 0.5791387476118786 1107 98-99 0.6332834941542842 1107 100-101 0.7812871205382059 1108 1 -0.05152644340991941 1108 2 -0.044377087133739224 1108 3 -0.004735784950220534 1108 4 0.046589096899097626 1108 5 -0.05359924741149058 1108 6 -0.037355982859175185 1108 7 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-0.7213242648282971 2316 96-97 -0.7232942431287483 2316 98-99 -0.7884738417179094 2316 100-101 -0.7613704949978519 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 6.0 4 22.0 5 32.0 6 63.0 7 213.0 8 454.0 9 879.0 10 1484.0 11 2434.0 12 3696.0 13 5558.0 14 7466.0 15 10374.0 16 13661.0 17 19430.0 18 19517.0 19 13413.0 20 11163.0 21 10994.0 22 11446.0 23 12185.0 24 13900.0 25 16064.0 26 18890.0 27 23098.0 28 28142.0 29 34703.0 30 41878.0 31 52037.0 32 65473.0 33 86404.0 34 121944.0 35 186607.0 36 306435.0 37 500098.0 38 430174.0 39 48723.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.36829050870925 18.861349016950367 15.381471768970242 22.388888705370142 2 15.858423285867342 19.829235714184573 40.154716785213644 24.157624214734437 3 19.683735408854126 23.9715289189503 29.77013783423835 26.574597837957224 4 13.205893720308307 16.939608856530864 36.24978705271042 33.60471037045042 5 13.859341059682343 37.14843641240564 33.82678902857811 15.165433499333906 6 32.23821404588524 35.87891663744786 16.957069354513703 14.92579996215319 7 28.665418634315355 33.008552340674136 19.833662801919143 18.492366223091366 8 26.744864824561443 34.672196789049494 18.956367938440657 19.626570447948406 9 26.30102375485002 16.95075462633939 19.998140686775564 36.750080932035026 10-11 25.32560289447866 26.665389376183597 26.27092369337667 21.738084035961077 12-13 26.368442934137587 23.90585205106582 27.068019021560886 22.65768599323571 14-15 23.04500230997669 25.17945380730878 25.859115488924616 25.916428393789914 16-17 22.132303130790433 28.477930483979847 26.640587560884736 22.749178824344977 18-19 22.123913850846762 26.6315773577925 29.42485591549874 21.819652875861994 20-21 22.77585109309796 25.561107259839112 29.883760273969372 21.77928137309356 22-23 22.611698816791716 25.463588019078188 29.806934082844872 22.117779081285224 24-25 22.419647093996343 25.642966007448504 29.85309465846166 22.0842922400935 26-27 21.85621782537597 26.01528026925144 29.825647846741347 22.30285405863124 28-29 21.979086098659366 26.359641899343533 29.663120927161877 21.998151074835224 30-31 22.102159014170848 25.907650954263406 29.57900379836617 22.41118623319958 32-33 21.897190711256574 25.904794084356762 29.5875599528942 22.610455251492468 34-35 22.554398296466793 26.063510561297008 29.36104996777437 22.02104117446182 36-37 22.620887277113585 25.669771213954206 29.419343238531027 22.289998270401185 38-39 22.10487960175147 26.142021648415497 29.279545678741968 22.47355307109106 40-41 22.556685518968486 25.89207037542401 29.324941911531845 22.22630219407566 42-43 22.189262053790017 25.93209880184767 29.194376766109574 22.68426237825274 44-45 22.272984897199684 26.1833468537917 29.184676247551334 22.358992001457285 46-47 22.703743955482338 26.44449048143312 28.442750094499047 22.409015468585498 48-49 22.469159805556117 26.45834090348167 28.454689299876502 22.617809991085707 50-51 22.603464068418724 26.716189987206647 28.293368455675584 22.38697748869905 52-53 22.864309751121837 26.538777170540463 28.152150779422474 22.44476229891523 54-55 22.572577863203133 26.72116858865851 28.170838231317617 22.535415316820735 56-57 22.54143211004455 26.854552636086886 28.079807689131407 22.524207564737157 58-59 22.6402962810555 26.486702415070013 28.071903274504038 22.801098029370443 60-61 22.359606978347568 26.807574458406023 28.250708211955345 22.582110351291064 62-63 22.324308458101175 26.7394549210054 28.487203578552656 22.449033042340762 64-65 22.165229164316973 26.8498807731131 28.279824772105112 22.70506529046482 66-67 22.129152000241614 26.800023972791827 28.107980438579393 22.962843588387162 68-69 22.463258594466335 26.619663276338123 28.26116399339898 22.655914135796557 70-71 22.447829683696 26.696841867820254 27.922710397820932 22.932618050662814 72-73 22.48952252144692 26.709984431842475 27.872641240091756 22.92785180661885 74-75 22.258831590665523 27.211761391441243 27.751786279729444 22.777620738163787 76-77 22.415881691440912 26.827724816888924 27.840882009977026 22.91551148169314 78-79 22.549808430022807 26.99671412304177 27.83510116942865 22.618376277506773 80-81 22.600467469440588 27.00242417778751 27.665404005249478 22.731704347522427 82-83 22.50559089864464 27.105110782140645 27.68855096271051 22.7007473565042 84-85 22.557406308016258 27.152294128695466 27.5981846308054 22.692114932482877 86-87 22.60929292147549 27.149763492333527 27.4242950132004 22.81664857299058 88-89 22.621525123308928 27.276566868075964 27.24166945090955 22.860238557705557 90-91 22.510675116625812 27.169950310597113 27.360718543828444 22.95865602894864 92-93 22.557252295648524 26.91568704760381 27.51427694356493 23.01278371318273 94-95 22.450448758393428 26.851390870235935 27.556205107054087 23.141955264316547 96-97 22.51276917192625 26.7020627223707 27.620721085703458 23.16444701999959 98-99 22.36781813145301 26.822557453296707 27.336509570948376 23.47311484430191 100-101 22.414064855839992 26.681080229100612 27.554695009931248 23.350159905128148 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1610.0 1 1039.0 2 664.5 3 905.5 4 702.0 5 578.5 6 1241.5 7 2956.5 8 4769.0 9 5404.5 10 4320.5 11 2878.0 12 2593.5 13 2704.0 14 2819.5 15 2985.5 16 3125.0 17 3363.0 18 3627.0 19 3804.0 20 4147.5 21 4818.5 22 5569.0 23 6514.0 24 7878.0 25 9839.5 26 12507.0 27 15531.0 28 17939.0 29 20312.0 30 23420.5 31 27780.5 32 32986.0 33 35775.5 34 37226.5 35 39481.5 36 42998.0 37 47007.0 38 50568.0 39 53309.0 40 56034.0 41 60190.5 42 62657.5 43 64469.5 44 67787.5 45 71011.0 46 72496.0 47 75995.5 48 79452.5 49 78567.0 50 77281.5 51 75789.0 52 74335.5 53 75271.5 54 77403.0 55 74010.0 56 67769.5 57 62536.5 58 58529.0 59 53153.5 60 43077.5 61 35087.5 62 29422.5 63 21859.5 64 15487.0 65 11711.5 66 8417.5 67 6279.5 68 4848.5 69 3564.5 70 2594.0 71 1645.5 72 1108.0 73 820.5 74 535.5 75 359.5 76 254.0 77 142.0 78 85.0 79 53.5 80 30.0 81 17.0 82 15.0 83 8.0 84 3.0 85 2.0 86 1.5 87 1.5 88 2.5 89 1.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.016941402096864237 3 0.006937008658047473 4 0.0 5 0.0 6 0.0 7 0.0 8 3.7752428071006653E-4 9 3.303337456213082E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.651668728106541E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005639268943106619 26-27 0.021448098197840656 28-29 0.0 30-31 0.0 32-33 4.48310083343204E-4 34-35 0.019513286259201564 36-37 0.003798838074645044 38-39 0.01849868975479326 40-41 0.015006590158225147 42-43 0.008565082118609634 44-45 0.0027370510351479825 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 9.438107017751663E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0024067172895266744 86-87 2.1235740789941242E-4 88-89 4.7190535088758317E-5 90-91 2.5954794298817073E-4 92-93 2.5954794298817073E-4 94-95 0.0 96-97 2.3595267544379158E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2119069.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.594455020534284 #Duplication Level Percentage of deduplicated Percentage of total 1 77.0997894550872 42.092209874984206 2 13.045880171974117 14.244654365042416 3 4.130703193950829 6.765404691759776 4 1.8180816636536 3.9702871043997843 5 0.9812274623940381 2.6784789280292154 6 0.6102821189135725 1.999081181451803 7 0.42740480948590215 1.6333752852926677 8 0.2768526187162849 1.2091694271858673 9 0.2213125089396512 1.0874192233308644 >10 1.250216359229823 12.470947576210714 >50 0.08757970442070144 3.291914830889833 >100 0.04531146843495806 4.575413003604211 >500 0.003629927767305055 1.395946587234868 >1k 0.00146925647724251 1.2112201755727172 >5k 8.642685160250058E-5 0.30981190778381235 >10k+ 1.7285370320500117E-4 1.0646658372272222 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12016 0.5670414696265199 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10548 0.49776576411622275 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6566 0.3098530533927871 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2744 0.12949082828355282 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 4.7190535088758317E-5 0.0 0.0 0.0 0.0 3 4.7190535088758317E-5 0.0 0.0 0.0 0.0 4 4.7190535088758317E-5 0.0 0.0 9.438107017751663E-5 0.0 5 4.7190535088758317E-5 0.0 0.0 9.438107017751663E-5 0.0 6 4.7190535088758317E-5 0.0 0.0 9.438107017751663E-5 0.0 7 9.438107017751663E-5 0.0 0.0 9.438107017751663E-5 0.0 8 9.438107017751663E-5 0.0 0.0 9.438107017751663E-5 0.0 9 9.438107017751663E-5 0.0 0.0 9.438107017751663E-5 0.0 10-11 9.438107017751663E-5 0.0 0.0 1.4157160526627496E-4 4.7190535088758317E-5 12-13 9.438107017751663E-5 0.0 0.0 1.4157160526627496E-4 9.438107017751663E-5 14-15 9.438107017751663E-5 0.0 0.0 1.4157160526627496E-4 1.4157160526627496E-4 16-17 9.438107017751663E-5 0.0 0.0 1.8876214035503327E-4 1.8876214035503327E-4 18-19 9.438107017751663E-5 0.0 0.0 1.8876214035503327E-4 1.8876214035503327E-4 20-21 9.438107017751663E-5 0.0 0.0 3.0673847807692904E-4 1.8876214035503327E-4 22-23 9.438107017751663E-5 0.0 0.0 3.7752428071006653E-4 1.8876214035503327E-4 24-25 1.4157160526627496E-4 0.0 0.0 7.078580263313748E-4 1.8876214035503327E-4 26-27 1.4157160526627496E-4 0.0 0.0 0.0013213349824852329 2.1235740789941242E-4 28-29 3.0673847807692904E-4 0.0 0.0 0.002878622640414257 2.359526754437916E-4 30-31 3.303337456213082E-4 0.0 0.0 0.009131368539674735 2.359526754437916E-4 32-33 3.303337456213082E-4 0.0 0.0 0.024019982360177983 3.303337456213082E-4 34-35 4.0111954825444566E-4 0.0 0.0 0.04473662726414289 3.303337456213082E-4 36-37 4.2471481579882484E-4 0.0 0.0 0.0792565036815696 3.303337456213082E-4 38-39 4.2471481579882484E-4 0.0 0.0 0.1299627336344404 3.303337456213082E-4 40-41 4.2471481579882484E-4 0.0 0.0 0.183830729438258 3.303337456213082E-4 42-43 4.48310083343204E-4 0.0 0.0 0.23755715363680935 3.303337456213082E-4 44-45 5.190958859763415E-4 0.0 0.0 0.30690364494973976 3.303337456213082E-4 46-47 5.190958859763415E-4 0.0 0.0 0.388189341640126 3.303337456213082E-4 48-49 5.190958859763415E-4 0.0 0.0 0.4717401840147725 3.303337456213082E-4 50-51 5.190958859763415E-4 0.0 0.0 0.558830316521076 3.303337456213082E-4 52-53 5.190958859763415E-4 0.0 0.0 0.6416733008693912 3.539290131656874E-4 54-55 5.190958859763415E-4 0.0 0.0 0.733388105814393 3.7752428071006653E-4 56-57 5.190958859763415E-4 0.0 0.0 0.8400151198474424 3.7752428071006653E-4 58-59 5.190958859763415E-4 0.0 0.0 0.9549712633236578 3.7752428071006653E-4 60-61 5.190958859763415E-4 0.0 0.0 1.0781149646377726 3.7752428071006653E-4 62-63 5.190958859763415E-4 0.0 0.0 1.2003148552501122 3.7752428071006653E-4 64-65 5.190958859763415E-4 0.0 0.0 1.3248270820818009 3.7752428071006653E-4 66-67 5.190958859763415E-4 0.0 0.0 1.473807601357011 3.7752428071006653E-4 68-69 5.662864210650998E-4 0.0 0.0 1.6328632998736707 3.7752428071006653E-4 70-71 5.662864210650998E-4 0.0 0.0 1.802702035658112 4.48310083343204E-4 72-73 5.898816886094789E-4 0.0 0.0 1.9717621276135888 5.426911535207206E-4 74-75 6.134769561538581E-4 0.0 0.0 2.1443379144331782 5.662864210650998E-4 76-77 6.134769561538581E-4 0.0 0.0 2.329655145726732 5.662864210650998E-4 78-79 6.134769561538581E-4 0.0 0.0 2.5444900567183044 5.662864210650998E-4 80-81 8.022390965088914E-4 0.0 0.0 2.7640676164862965 6.134769561538581E-4 82-83 8.022390965088914E-4 0.0 0.0 2.9908417328553245 6.606674912426164E-4 84-85 8.258343640532705E-4 0.0 0.0 3.2204472813296783 6.606674912426164E-4 86-87 8.730248991420289E-4 0.0 0.0 3.4587358882603634 6.842627587869956E-4 88-89 8.96620166686408E-4 0.0 0.0 3.7238523143890077 7.078580263313748E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6680 0.0 45.51443 1 GTATCAA 10680 0.0 35.495823 1 TATCAAC 13150 0.0 28.756294 2 ATCAACG 13245 0.0 28.405233 3 TCAACGC 13470 0.0 27.926804 4 CAACGCA 13600 0.0 27.729702 5 AACGCAG 14005 0.0 26.927809 6 CGCAGAG 15665 0.0 23.922703 8 ACGCAGA 15730 0.0 23.884237 7 GAGTACT 9460 0.0 23.422009 12-13 GTACTTT 9715 0.0 22.53833 14-15 GTGTAAG 925 0.0 22.083725 1 GCAGAGT 17160 0.0 21.395666 9 TGGTATC 3720 0.0 21.326479 2 TACATGG 8805 0.0 21.203562 2 GTACATG 9020 0.0 21.066824 1 GTACCTG 1770 0.0 20.397923 1 AGTACTT 9860 0.0 20.352295 12-13 CAGAGTA 16405 0.0 20.179955 10-11 GTGGTAT 4010 0.0 20.139568 1 >>END_MODULE