FastQCFastQC Report
Thu 26 May 2016
SRR938144_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938144_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2152016
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121030.5624028817629608No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102010.47402063925175275No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62110.28861309581341404No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27100.1259284317588717No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65600.048.0365561
GTATCAA108450.038.0546071
TATCAAC134200.030.4370752
ATCAACG134950.030.1981493
TCAACGC137100.029.7592664
CAACGCA139200.029.2761525
AACGCAG143150.028.494246
ACGCAGA160250.025.5396927
CGCAGAG161200.025.2093188
GAGTACT96650.024.1230712-13
GTACATG96750.024.0588871
TACATGG95750.023.589412
GCAGAGT171150.022.9661739
GTACTTT102400.022.55856714-15
TATAACG1903.2556636E-722.5242942
TAAGGTG11100.021.8483985
ACATGGG99900.021.7055973
GTAAGGT10750.021.2326984
AGTACTT101400.021.07109612-13
AGAGTAC147850.020.99166110-11
Produced by FastQC (version 0.11.5)