##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938144_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2152016 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.854135378175627 33.0 31.0 34.0 30.0 34.0 2 31.9845939807139 33.0 31.0 34.0 30.0 34.0 3 31.994943810826687 34.0 31.0 34.0 30.0 34.0 4 35.52176238466628 37.0 35.0 37.0 33.0 37.0 5 35.520855792893734 37.0 35.0 37.0 33.0 37.0 6 35.55009209968699 37.0 35.0 37.0 33.0 37.0 7 35.47781057389908 37.0 35.0 37.0 33.0 37.0 8 35.47232873733281 37.0 35.0 37.0 33.0 37.0 9 37.083937572954845 39.0 37.0 39.0 33.0 39.0 10-11 36.97979685095278 39.0 37.0 39.0 33.0 39.0 12-13 36.81823578449231 39.0 37.0 39.0 33.0 39.0 14-15 37.950296605601444 40.0 38.0 41.0 33.0 41.0 16-17 37.75332316302481 40.0 38.0 41.0 32.5 41.0 18-19 37.95016324228072 40.0 38.0 41.0 33.0 41.0 20-21 37.950150695905606 40.0 38.0 41.0 33.0 41.0 22-23 37.82948291276645 40.0 38.0 41.0 32.5 41.0 24-25 37.69296836083004 40.0 38.0 41.0 32.0 41.0 26-27 37.49353327298682 40.0 37.0 41.0 32.0 41.0 28-29 37.29292649311158 40.0 37.0 41.0 31.0 41.0 30-31 37.04184355506651 40.0 36.5 41.0 31.0 41.0 32-33 36.71689197478086 39.0 36.0 41.0 30.0 41.0 34-35 36.44859122794626 39.0 35.0 41.0 30.0 41.0 36-37 36.154666136311256 39.0 35.0 40.5 29.5 41.0 38-39 35.83114553981011 39.0 35.0 40.0 28.5 41.0 40-41 35.68325026393856 39.0 35.0 40.0 27.5 41.0 42-43 35.42927376004639 38.5 35.0 40.0 26.5 41.0 44-45 35.048339092274404 38.0 34.5 40.0 24.5 41.0 46-47 34.604908838967745 38.0 34.0 40.0 23.5 41.0 48-49 34.14137859569817 38.0 33.5 40.0 22.0 41.0 50-51 33.39603794767325 37.5 32.5 39.5 20.0 40.5 52-53 33.455811666827756 38.0 33.0 39.5 20.0 40.5 54-55 33.50134339149895 38.0 33.0 40.0 18.5 41.0 56-57 33.145475451855376 37.5 33.0 40.0 14.0 41.0 58-59 32.701618854134914 37.0 32.0 40.0 10.5 41.0 60-61 32.157485353268754 36.0 31.0 39.5 7.5 41.0 62-63 31.56706595118252 36.0 31.0 39.0 3.5 40.5 64-65 30.991352991799317 35.0 30.0 39.0 2.0 40.0 66-67 30.362249165433713 35.0 29.0 38.0 2.0 40.0 68-69 29.632797804477292 34.5 28.0 37.5 2.0 40.0 70-71 28.998863623690532 34.0 27.5 37.0 2.0 39.0 72-73 28.265052397379947 34.0 26.0 36.0 2.0 39.0 74-75 27.54577916706939 33.5 25.5 36.0 2.0 38.5 76-77 26.76442995776983 33.0 23.0 35.0 2.0 37.5 78-79 25.96887499907064 32.0 20.5 35.0 2.0 37.0 80-81 25.20298478264102 31.5 18.5 35.0 2.0 36.0 82-83 24.41827175076765 31.0 11.0 34.5 2.0 36.0 84-85 23.64479817993918 30.5 4.5 34.0 2.0 35.0 86-87 22.853128415402114 30.0 2.0 34.0 2.0 35.0 88-89 21.92523475661891 29.0 2.0 34.0 2.0 35.0 90-91 20.98009935799734 29.0 2.0 34.0 2.0 35.0 92-93 19.993578811681697 26.5 2.0 33.5 2.0 35.0 94-95 18.871320891666233 25.5 2.0 33.0 2.0 35.0 96-97 17.57233124660783 22.0 2.0 32.5 2.0 34.0 98-99 16.017125569698365 6.5 2.0 32.0 2.0 34.0 100-101 12.360718507669088 2.0 2.0 27.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19216492268943242 1101 2 0.17561805424378463 1101 3 0.16882765723880766 1101 4 0.06830184783508741 1101 5 0.17439675824799394 1101 6 0.1487857083988402 1101 7 0.17490512979377115 1101 8 0.22668273286515728 1101 9 0.21986742227618805 1101 10-11 0.28745416685678293 1101 12-13 0.40627230428673755 1101 14-15 0.3850713533094421 1101 16-17 0.3117033736816879 1101 18-19 0.29123818625211584 1101 20-21 0.3240585520674273 1101 22-23 0.32796693852164793 1101 24-25 0.3294377429507733 1101 26-27 0.40483246498870784 1101 28-29 0.3562125899826114 1101 30-31 0.37482385011632857 1101 32-33 0.32853403214654975 1101 34-35 0.3758094927641906 1101 36-37 0.4493602085975894 1101 38-39 0.5455236395084171 1101 40-41 0.5262046448660911 1101 42-43 0.30922526058755295 1101 44-45 0.30044293808301603 1101 46-47 0.4188525988372831 1101 48-49 0.4708824366720066 1101 50-51 0.33975607597459145 1101 52-53 0.3214768832744852 1101 54-55 0.3979122002407536 1101 56-57 0.47388914117696856 1101 58-59 0.5259260586275261 1101 60-61 0.4748998529611086 1101 62-63 0.3158186408121537 1101 64-65 0.22704680105386643 1101 66-67 0.16436906544126373 1101 68-69 0.1713973796822721 1101 70-71 0.20555035222064433 1101 72-73 0.1895718406213831 1101 74-75 0.19592457396903384 1101 76-77 -0.1978438519795489 1101 78-79 -0.10355853398557358 1101 80-81 -0.075580461462458 1101 82-83 -0.06018769964429538 1101 84-85 -0.043683274518780735 1101 86-87 0.23414040659671187 1101 88-89 0.24232077592144208 1101 90-91 0.06099584632348254 1101 92-93 -0.08741056129347058 1101 94-95 -0.4508362574016722 1101 96-97 -0.5371244084205848 1101 98-99 -0.4550163168931167 1101 100-101 -0.624281926147269 1102 1 0.2119789636302336 1102 2 0.2648548998186584 1102 3 0.20201768056967495 1102 4 0.18101000561367186 1102 5 0.2758906217136854 1102 6 0.21045830809961075 1102 7 0.2702143067930862 1102 8 0.3623537389587028 1102 9 0.3267623531147805 1102 10-11 0.3826971346349666 1102 12-13 0.4632010562859108 1102 14-15 0.48343675942133046 1102 16-17 0.477340501290648 1102 18-19 0.3920375694458613 1102 20-21 0.4429029575315582 1102 22-23 0.5908178330626797 1102 24-25 0.5530881926575546 1102 26-27 0.4235851520512526 1102 28-29 0.3811195817910189 1102 30-31 0.4184565867068102 1102 32-33 0.45969163302246585 1102 34-35 0.4678503418472957 1102 36-37 0.401365115532073 1102 38-39 0.3110704818158254 1102 40-41 0.535185596100554 1102 42-43 0.6742458685659045 1102 44-45 0.5192085230457764 1102 46-47 0.6115426422315551 1102 48-49 0.4053192570243169 1102 50-51 0.4165235182349889 1102 52-53 0.3354252458143918 1102 54-55 0.3784105751053275 1102 56-57 0.48227936858127407 1102 58-59 0.46063631446453 1102 60-61 0.5276393669682236 1102 62-63 0.4246935426448033 1102 64-65 0.21865428068951687 1102 66-67 0.0030834027471513537 1102 68-69 0.06901155476123932 1102 70-71 -0.0598844216648331 1102 72-73 -0.11061417576181043 1102 74-75 -0.07183554801644121 1102 76-77 -0.468062313747307 1102 78-79 -0.5188339471483125 1102 80-81 -0.41592566659639374 1102 82-83 -0.7129661939720862 1102 84-85 -0.5313256530062276 1102 86-87 -0.276713033129969 1102 88-89 -0.41275211154581015 1102 90-91 -0.3984391109987975 1102 92-93 -0.5874317711738364 1102 94-95 -0.8450754819225885 1102 96-97 -0.9256598948433954 1102 98-99 -0.8080954756060805 1102 100-101 -0.8871090312278955 1103 1 0.42588024284797044 1103 2 0.2639936045269806 1103 3 0.39654986193730224 1103 4 0.23280559867508543 1103 5 0.38279973697723335 1103 6 0.28692605975723495 1103 7 0.2612035238582564 1103 8 0.3656234462790451 1103 9 0.43410278577166395 1103 10-11 0.4643895865805874 1103 12-13 0.3564138350683095 1103 14-15 0.4581665163029882 1103 16-17 0.488918426412873 1103 18-19 0.398503981608485 1103 20-21 0.5649671193425547 1103 22-23 0.5259206393047222 1103 24-25 0.38857141548255214 1103 26-27 0.430247800232884 1103 28-29 0.3892744274142146 1103 30-31 0.4197846960096072 1103 32-33 0.5353583666638784 1103 34-35 0.41153998085339083 1103 36-37 0.4250025142327658 1103 38-39 0.4996948204401619 1103 40-41 0.6020578752513188 1103 42-43 0.6399407316025361 1103 44-45 0.5458771989549618 1103 46-47 0.7461685882499012 1103 48-49 0.7447379161898198 1103 50-51 0.7556153406179575 1103 52-53 0.6899607759195447 1103 54-55 0.5903093092825387 1103 56-57 0.5793565904686275 1103 58-59 0.644587824745166 1103 60-61 0.6216538335143369 1103 62-63 0.5104192140666726 1103 64-65 0.4485464513411799 1103 66-67 0.34312559683998245 1103 68-69 0.38091765616101014 1103 70-71 0.21545078696151165 1103 72-73 0.3110119711807897 1103 74-75 0.244024742653977 1103 76-77 0.2568516236982781 1103 78-79 0.02250822685209286 1103 80-81 0.09892397508868811 1103 82-83 -0.03723516457016984 1103 84-85 0.06512468521626147 1103 86-87 -0.0031861427676354026 1103 88-89 -0.051975204439788314 1103 90-91 -0.06752356615920618 1103 92-93 -0.32505349778290693 1103 94-95 -0.3222397709856111 1103 96-97 -0.47450458039694254 1103 98-99 -0.8232813305387694 1103 100-101 -0.857525614176124 1104 1 0.3743466884979547 1104 2 0.21320146066521772 1104 3 0.26309572289771666 1104 4 0.2765126511163132 1104 5 0.24262342594649056 1104 6 0.26561703825834115 1104 7 0.30616007398885614 1104 8 0.3100058553608207 1104 9 0.310227961580388 1104 10-11 0.31517067960850653 1104 12-13 0.27426256422241835 1104 14-15 0.4142899340245023 1104 16-17 0.3411632526133914 1104 18-19 0.32952103505432717 1104 20-21 0.3535015025051038 1104 22-23 0.41890976427961135 1104 24-25 0.41112390694327416 1104 26-27 0.3460553901464962 1104 28-29 0.2810369098333396 1104 30-31 0.28189904702427526 1104 32-33 0.35937747958330846 1104 34-35 0.213716743963694 1104 36-37 0.36910651764846847 1104 38-39 0.44319680456715105 1104 40-41 0.30679604583600906 1104 42-43 0.328842429412461 1104 44-45 0.41057757066482736 1104 46-47 0.14505785387176218 1104 48-49 0.3345024580825253 1104 50-51 0.2172179626794346 1104 52-53 0.3806468066609483 1104 54-55 0.26333121002596016 1104 56-57 0.237719183396095 1104 58-59 0.27965086472908496 1104 60-61 0.3123615891904521 1104 62-63 0.3868132516395093 1104 64-65 0.2243917145519525 1104 66-67 0.1957421329058846 1104 68-69 0.11267888280356786 1104 70-71 -0.11243071796113213 1104 72-73 0.006978416830776268 1104 74-75 -0.11105512473601564 1104 76-77 -0.3012799063399001 1104 78-79 -0.27954823286259867 1104 80-81 -0.47945079887152886 1104 82-83 -0.7312366966175858 1104 84-85 -0.571669568350142 1104 86-87 -0.7122454405373766 1104 88-89 -0.6638268469955442 1104 90-91 -0.9712400369247227 1104 92-93 -1.28612555424262 1104 94-95 -1.715627730599838 1104 96-97 -1.5626638630973666 1104 98-99 -1.553234364494969 1104 100-101 -1.5835210682649326 1105 1 0.18919008521605107 1105 2 0.26511114696047144 1105 3 0.3801776574742206 1105 4 0.2658035388861961 1105 5 0.2615885566631917 1105 6 0.22663866725437742 1105 7 0.36190556347710157 1105 8 0.3471569804978216 1105 9 0.41612822447002884 1105 10-11 0.3540949206392199 1105 12-13 0.3689654673134797 1105 14-15 0.3006521986309778 1105 16-17 0.4308288347644904 1105 18-19 0.49026849334072153 1105 20-21 0.3486852522080994 1105 22-23 0.3803334161079519 1105 24-25 0.48548723307204966 1105 26-27 0.4509134115378899 1105 28-29 0.4652968956158432 1105 30-31 0.35574897670321093 1105 32-33 0.25437431321938675 1105 34-35 0.2629618463986887 1105 36-37 0.44770407710522164 1105 38-39 0.39351423135276775 1105 40-41 0.5106999426192473 1105 42-43 0.6314292193996636 1105 44-45 0.45935897562983996 1105 46-47 0.5314841882474539 1105 48-49 0.6205275121166949 1105 50-51 0.5858696664802281 1105 52-53 0.6524110078750027 1105 54-55 0.7202744090019948 1105 56-57 0.8930481355547784 1105 58-59 0.7430975644294122 1105 60-61 0.6922078983885172 1105 62-63 0.7126520985656981 1105 64-65 0.363186355013962 1105 66-67 0.37270355404282185 1105 68-69 0.2799135549196343 1105 70-71 0.13112278544570444 1105 72-73 0.30163229157881233 1105 74-75 0.1146453075373266 1105 76-77 -0.2732509990180745 1105 78-79 -0.4427707003462906 1105 80-81 -0.5105240381624156 1105 82-83 -0.6456048752829062 1105 84-85 -0.7209408735243947 1105 86-87 -0.7822430406155512 1105 88-89 -0.6471786467932041 1105 90-91 -0.7880034908577223 1105 92-93 -0.9206599741992392 1105 94-95 -0.9197328184340989 1105 96-97 -1.0517442188031403 1105 98-99 -1.1141940553888698 1105 100-101 -1.2635890947914934 1106 1 0.3214445467800253 1106 2 0.32839000019586706 1106 3 0.3210982101765225 1106 4 0.3163280988265029 1106 5 0.3281449950206934 1106 6 0.306144493259481 1106 7 0.2537159902947721 1106 8 0.07988747228191073 1106 9 0.3453821348924535 1106 10-11 0.381695894160039 1106 12-13 0.44100161829700113 1106 14-15 0.4745471110579942 1106 16-17 0.04831465384373956 1106 18-19 0.3135471514817638 1106 20-21 0.5069165268082827 1106 22-23 0.4460265619556125 1106 24-25 0.38141658940701006 1106 26-27 0.34930095671442274 1106 28-29 0.3340422926084585 1106 30-31 0.35072285844676543 1106 32-33 0.3063815902387361 1106 34-35 0.32532863353610963 1106 36-37 0.4777315366353321 1106 38-39 0.45535670241783066 1106 40-41 0.34658657329101317 1106 42-43 0.36790552275186883 1106 44-45 0.5213160646270083 1106 46-47 0.4671640189055637 1106 48-49 0.6648797744795729 1106 50-51 0.5179817091668681 1106 52-53 0.5664890163518521 1106 54-55 0.5179640041324447 1106 56-57 0.38220088955543474 1106 58-59 0.5632742223655995 1106 60-61 0.8112081373791042 1106 62-63 0.7024852441207088 1106 64-65 0.3271426733884333 1106 66-67 0.11055283390079396 1106 68-69 0.08818701867354406 1106 70-71 0.017636400592827783 1106 72-73 0.0480754776646819 1106 74-75 0.033790891820526525 1106 76-77 -0.4324138491514731 1106 78-79 -0.5243933000760812 1106 80-81 -0.29383761819255483 1106 82-83 -0.26964830328576994 1106 84-85 -0.16657539351912476 1106 86-87 -0.22521940005738728 1106 88-89 -0.8065902422260436 1106 90-91 -0.5335394882044397 1106 92-93 -0.714052616215735 1106 94-95 -0.8368908790011425 1106 96-97 -1.122356181013366 1106 98-99 -1.2016475375791948 1106 100-101 -1.2202537617410467 1107 1 0.11885727050853845 1107 2 0.049635478716886894 1107 3 0.12290158968887965 1107 4 0.13220826269427732 1107 5 0.10548912778278208 1107 6 0.12315672079802198 1107 7 0.26389622400441937 1107 8 0.17514552233871683 1107 9 0.14328896375903355 1107 10-11 0.1640696836545743 1107 12-13 0.11203392276583202 1107 14-15 0.08995714535347332 1107 16-17 0.10321974187576899 1107 18-19 0.06581596969485304 1107 20-21 0.09064649939274716 1107 22-23 0.3615616861594688 1107 24-25 0.20914172518168073 1107 26-27 0.12806694476834224 1107 28-29 0.14576321702288197 1107 30-31 0.16696265580111458 1107 32-33 0.26358207808937806 1107 34-35 0.0396368342220228 1107 36-37 0.04165456464505013 1107 38-39 -0.12209167193456238 1107 40-41 0.0746035855185525 1107 42-43 -0.0163081308116233 1107 44-45 0.09049703845193591 1107 46-47 0.10824354481480469 1107 48-49 -0.05568292162491417 1107 50-51 0.024179914126683855 1107 52-53 -0.07651063965090543 1107 54-55 -0.027689642759597177 1107 56-57 0.10587102746705312 1107 58-59 -0.0673748900172555 1107 60-61 -0.029954781802395303 1107 62-63 -0.1501119351018545 1107 64-65 -0.24387295242595286 1107 66-67 -0.21249393930077787 1107 68-69 -0.24323556630941212 1107 70-71 -0.3151131798347677 1107 72-73 -0.25866895322835504 1107 74-75 -0.5560403410447599 1107 76-77 -0.5379813924899146 1107 78-79 -0.6632472680322188 1107 80-81 -0.5410423799484008 1107 82-83 -0.6744663495898848 1107 84-85 -0.6020383825424851 1107 86-87 -0.5247211252557094 1107 88-89 -0.8676851958906582 1107 90-91 -0.9193230201180036 1107 92-93 -0.7557013012080738 1107 94-95 -0.7423677321560156 1107 96-97 -0.9355260635330929 1107 98-99 -0.9596360918525768 1107 100-101 -1.1674856263103717 1108 1 0.03797160223156837 1108 2 -0.035720209452101415 1108 3 0.0708536594056568 1108 4 0.1803014709920916 1108 5 0.12062032035643 1108 6 0.1316591362569568 1108 7 0.0900532761972741 1108 8 0.0036971942151993176 1108 9 0.14402023211178516 1108 10-11 0.17975832626137134 1108 12-13 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66-67 -0.7893171918273545 2316 68-69 -0.8862635244268837 2316 70-71 -0.9847017138734309 2316 72-73 -0.9166625557232351 2316 74-75 -0.7166949559193263 2316 76-77 -0.9460904852537375 2316 78-79 -0.7441458333297604 2316 80-81 -0.6937547618167912 2316 82-83 -0.9438858379352055 2316 84-85 -0.9372997111541999 2316 86-87 -0.9526690416946728 2316 88-89 -1.0550606886369955 2316 90-91 -0.8820812337713555 2316 92-93 -0.9246785018988284 2316 94-95 -1.0339183620698158 2316 96-97 -1.247388407876258 2316 98-99 -1.2500660654027556 2316 100-101 -1.0066087746406822 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8042.0 3 2620.0 4 2539.0 5 2818.0 6 3363.0 7 4023.0 8 5231.0 9 6494.0 10 8150.0 11 9912.0 12 12381.0 13 14964.0 14 17186.0 15 21085.0 16 24813.0 17 30491.0 18 30515.0 19 25800.0 20 26806.0 21 28471.0 22 30935.0 23 34691.0 24 38743.0 25 42773.0 26 48049.0 27 54755.0 28 61644.0 29 71064.0 30 82423.0 31 96532.0 32 115847.0 33 140233.0 34 175927.0 35 224005.0 36 266003.0 37 258632.0 38 117699.0 39 6357.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.0167310664426 18.45164106575851 15.0260817929121 22.505546074886787 2 15.622169820969964 19.56610275397922 40.64481125321025 24.166916171840565 3 19.617744816346438 24.068841115910747 29.486395498084022 26.82701856965879 4 12.923941045947293 16.64702431107908 36.522870355335094 33.90616428763853 5 13.867414195505608 37.046907248413206 33.75514519686183 15.33053335921936 6 32.097892850890425 35.71560936725929 17.212450692263854 14.974047089586428 7 28.57008542791688 33.031243512849564 19.880864500357927 18.517806558875634 8 26.703831015676734 34.47881557703325 19.117272307909793 19.700081099380224 9 26.53678785597605 16.94089851893863 20.01426486400225 36.50804876108307 10-11 25.27426063185128 26.57217992809184 26.311554123811444 21.842005316245437 12-13 26.3929545071664 23.911241645251394 27.023987587363607 22.6718162602186 14-15 23.17382778873348 25.106628113340822 25.845998572397765 25.87354552552793 16-17 22.20256023570808 28.3674693441298 26.54910267046795 22.880867749694175 18-19 22.203209665895745 26.541430402372647 29.376788049495513 21.8785718822361 20-21 22.886233175958807 25.47380450458897 29.82526099642202 21.8147013230302 22-23 22.647363507904473 25.419550384456908 29.747722706818724 22.185363400819895 24-25 22.34482469579633 25.63122552283914 29.800247740301106 22.22370204106342 26-27 21.866651774963092 25.961684112064727 29.769771935886563 22.401892177085625 28-29 22.013719681704128 26.35513727358547 29.586180938073003 22.044962106637396 30-31 22.16220427575541 25.872332013512178 29.4649200839569 22.500543626775507 32-33 21.920312022380898 25.917738200641782 29.524136092452867 22.637813684524456 34-35 22.591594916549866 26.064088941540138 29.236652245712957 22.107663896197042 36-37 22.627124812310523 25.62641253990109 29.338484266639632 22.407978381148755 38-39 22.14185573969721 26.124285506068247 29.175481732038595 22.55837702219595 40-41 22.614839428569965 25.91335556940628 29.22289132958217 22.248913672441585 42-43 22.24595919333356 25.940882620096634 29.09136687988093 22.721791306688875 44-45 22.313705163725615 26.187103702188587 29.03758902173711 22.461602112348686 46-47 22.699510719174008 26.464657782373248 28.346580427279218 22.48925107117353 48-49 22.509139577998813 26.43413739305392 28.373578158732993 22.68314487021427 50-51 22.61308343343014 26.75405755301024 28.168578289031537 22.464280724528084 52-53 22.829900410054375 26.57130958036127 28.04212748591397 22.55666252367039 54-55 22.515598880548872 26.755198057721145 28.112319843710154 22.616883218019826 56-57 22.521731734048753 26.920205887903204 27.96221824972972 22.595844128318323 58-59 22.63732682245991 26.52139477935257 27.917206275526084 22.924072122661435 60-61 22.363736943151867 26.761924905981395 28.17880502238317 22.695533128483568 62-63 22.354225470309032 26.700809392279325 28.371154410325484 22.573810727086162 64-65 22.21009946805024 26.821717570612456 28.20798059450255 22.760202366834747 66-67 22.200282874253446 26.764739038622682 27.998995877752762 23.035982209371113 68-69 22.476942531691304 26.479057339039468 28.162533267644722 22.8814668616245 70-71 22.487844738105395 26.602047826001048 27.82676819395416 23.083339241939402 72-73 22.48196016067028 26.61829696849802 27.75481672601877 23.144926144812935 74-75 22.319404848103076 27.062303366150676 27.705641088818357 22.91265069692789 76-77 22.519958894692984 26.688145785334928 27.654032191720656 23.13786312825144 78-79 22.628171133741752 26.87158648364882 27.55820552279117 22.942036859818263 80-81 22.716785251482232 26.870127781568442 27.38644582065216 23.026641146297163 82-83 22.63004432356083 26.9157396861679 27.406917152611506 23.047298837659756 84-85 22.675285996733592 26.912787611812245 27.322064809504376 23.08986158194979 86-87 22.727559003879946 26.884527742368803 27.181072278082546 23.206840975668708 88-89 22.766475664018234 26.89931232536696 27.002842487681235 23.33136952293357 90-91 22.664358372648067 26.86394625703519 27.001294868279075 23.470400502037666 92-93 22.82517552765261 26.49466499180786 27.133875383621575 23.54628409691796 94-95 22.652899362688256 26.509136491299945 27.134357239169898 23.703606906841898 96-97 22.829134485653658 26.191421140039633 27.181409424325764 23.79803494998095 98-99 22.667302717349862 26.304043385351868 26.79593652883709 24.232717368461184 100-101 23.471845952161324 25.152446081744273 26.879458775987835 24.49624919010656 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1526.0 1 1019.0 2 689.0 3 865.5 4 713.0 5 672.5 6 1164.5 7 2639.0 8 4459.5 9 5083.0 10 4130.5 11 2931.5 12 2624.5 13 2704.5 14 2856.0 15 3034.0 16 3210.0 17 3405.5 18 3638.5 19 3908.0 20 4360.5 21 5070.5 22 5957.0 23 7054.0 24 8511.0 25 10474.5 26 13059.0 27 16125.5 28 18479.0 29 20841.5 30 23746.5 31 27777.0 32 32875.0 33 35826.0 34 37382.0 35 39804.5 36 43758.5 37 47943.5 38 51059.5 39 53588.0 40 56334.5 41 59888.0 42 62270.0 43 64459.5 44 67158.5 45 70342.5 46 72508.0 47 74915.0 48 77742.5 49 77960.0 50 77110.5 51 75800.5 52 74945.0 53 75420.5 54 76382.0 55 73865.0 56 68726.0 57 63992.0 58 60172.5 59 54581.0 60 45688.5 61 38038.5 62 31682.0 63 24399.5 64 18267.5 65 14310.0 66 10763.0 67 8047.5 68 6228.5 69 4638.0 70 3399.5 71 2359.5 72 1657.5 73 1178.0 74 832.0 75 578.0 76 399.0 77 271.0 78 173.0 79 104.0 80 66.0 81 41.0 82 27.0 83 21.5 84 17.5 85 13.5 86 7.5 87 5.5 88 5.0 89 3.0 90 2.5 91 5.0 92 4.0 93 2.0 94 1.0 95 1.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14865131114266808 2 0.05074311715154534 3 0.1506494375506502 4 0.12774068594285543 5 0.1923312837822767 6 0.198883279678218 7 0.16440398212652696 8 0.07286191180734716 9 0.1244879220228846 10-11 0.10178827666708797 12-13 0.15046356532665184 14-15 0.13745250964676842 16-17 0.1355473193507855 18-19 0.13250366168281277 20-21 0.13459472420279403 22-23 0.19428294213425923 24-25 0.21403186593408227 26-27 0.14723403543468078 28-29 0.12523141091887793 30-31 0.0977455557951242 32-33 0.12467379424688292 34-35 0.09384223909115919 36-37 0.08622147790722745 38-39 0.051370435907539716 40-41 0.06071051516345603 42-43 0.06331272629943271 44-45 0.039776655935643605 46-47 0.05130073382354035 48-49 0.04330822819161196 50-51 0.05490200816350808 52-53 0.02760202526375269 54-55 0.05299681786752515 56-57 0.025743303023769343 58-59 0.05355443453952015 60-61 0.03931197537564776 62-63 0.053112988007524106 64-65 0.04056661288763653 66-67 0.04105452747563215 68-69 0.06809893606738983 70-71 0.055691965115501 72-73 0.05350796648352057 74-75 0.04500431223559676 76-77 0.021793518263804733 78-79 0.021328837703808893 80-81 0.024721005791778498 82-83 0.02098032728381202 84-85 0.019423647407825963 86-87 0.0264170898357633 88-89 0.06668166035940254 90-91 0.11043133508301053 92-93 0.08294547995925682 94-95 0.07692786670731072 96-97 0.06902829718738152 98-99 0.05780626166348205 100-101 0.1307378755548286 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2152016.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.83827393506182 #Duplication Level Percentage of deduplicated Percentage of total 1 78.74065890791688 44.754831408354455 2 12.209582960395705 13.879432418716684 3 3.8071551658543648 6.491763846903482 4 1.6799333075125285 3.819380381001262 5 0.9202177576903324 2.615179449575573 6 0.5469486641532778 1.8652570800936081 7 0.37548561817328213 1.4939368097086327 8 0.25580209851013636 1.163147979862624 9 0.2063385921265207 1.0555136480395955 >10 1.1324680272869032 11.702142054213192 >50 0.07961781834839107 3.1281766042574195 >100 0.041124416807363486 4.324180761546345 >500 0.003029238829760941 1.246373217505792 >1k 0.0013918124352955436 1.133760344657659 >5k 8.187131972326727E-5 0.28902419556184206 >10k+ 1.6374263944653455E-4 1.0378998000018236 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12103 0.5624028817629608 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10201 0.47402063925175275 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6211 0.28861309581341404 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2710 0.1259284317588717 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 4.646805599958365E-5 0.0 0.0 0.0 0.0 6 4.646805599958365E-5 0.0 0.0 0.0 0.0 7 9.29361119991673E-5 0.0 0.0 0.0 0.0 8 9.29361119991673E-5 0.0 0.0 4.646805599958365E-5 0.0 9 9.29361119991673E-5 0.0 0.0 4.646805599958365E-5 0.0 10-11 1.3940416799875093E-4 0.0 0.0 4.646805599958365E-5 0.0 12-13 1.3940416799875093E-4 0.0 0.0 4.646805599958365E-5 4.646805599958365E-5 14-15 1.3940416799875093E-4 0.0 0.0 4.646805599958365E-5 4.646805599958365E-5 16-17 1.3940416799875093E-4 0.0 0.0 9.29361119991673E-5 4.646805599958365E-5 18-19 1.3940416799875093E-4 0.0 0.0 9.29361119991673E-5 9.29361119991673E-5 20-21 1.3940416799875093E-4 0.0 0.0 1.3940416799875093E-4 9.29361119991673E-5 22-23 1.858722239983346E-4 0.0 0.0 2.3234027999791823E-4 9.29361119991673E-5 24-25 2.7880833599750187E-4 0.0 0.0 6.970208399937547E-4 1.1617013999895912E-4 26-27 3.0204236399729366E-4 0.0 0.0 0.0011384673719897994 1.3940416799875093E-4 28-29 3.717444479966692E-4 0.0 0.0 0.0026719132199760597 1.3940416799875093E-4 30-31 4.6468055999583646E-4 0.0 0.0 0.009525951479914647 1.3940416799875093E-4 32-33 4.6468055999583646E-4 0.0 0.0 0.024256325231782665 1.858722239983346E-4 34-35 5.111486159954202E-4 0.0 0.0 0.044632567787600094 2.3234027999791823E-4 36-37 6.040847279945874E-4 0.0 0.0 0.07811280213530011 2.3234027999791823E-4 38-39 6.040847279945874E-4 0.0 0.0 0.12936706790284086 2.3234027999791823E-4 40-41 6.040847279945874E-4 0.0 0.0 0.18313060869435915 2.555743079977101E-4 42-43 6.040847279945874E-4 0.0 0.0 0.23712648976587536 2.7880833599750187E-4 44-45 6.50552783994171E-4 0.0 0.0 0.3061547869532568 3.0204236399729366E-4 46-47 6.737868119939628E-4 0.0 0.0 0.38666069397253555 3.252763919970855E-4 48-49 6.970208399937547E-4 0.0 0.0 0.4716275343677742 4.182125039962528E-4 50-51 6.970208399937547E-4 0.0 0.0 0.558034884499 4.182125039962528E-4 52-53 6.970208399937547E-4 0.0 0.0 0.642188533914246 4.879145879956283E-4 54-55 6.970208399937547E-4 0.0 0.0 0.7331032854774313 5.111486159954202E-4 56-57 6.970208399937547E-4 0.0 0.0 0.8416061962364592 5.111486159954202E-4 58-59 6.970208399937547E-4 0.0 0.0 0.9568934431714262 5.111486159954202E-4 60-61 6.970208399937547E-4 0.0 0.0 1.0805914082423178 5.111486159954202E-4 62-63 6.970208399937547E-4 0.0 0.0 1.199874907993249 5.111486159954202E-4 64-65 6.970208399937547E-4 0.0 0.0 1.326662998788113 5.576166719950037E-4 66-67 7.434888959933384E-4 0.0 0.0 1.4679491230548471 6.273187559943793E-4 68-69 7.434888959933384E-4 0.0 0.0 1.6286124266734077 6.50552783994171E-4 70-71 7.434888959933384E-4 0.0 0.0 1.7999866171998722 6.50552783994171E-4 72-73 7.434888959933384E-4 0.0 0.0 1.9722204667623289 6.50552783994171E-4 74-75 7.434888959933384E-4 0.0 0.0 2.1422703176928053 6.50552783994171E-4 76-77 7.434888959933384E-4 2.3234027999791824E-5 0.0 2.324657437491171 6.50552783994171E-4 78-79 7.434888959933384E-4 4.646805599958365E-5 0.0 2.5289774797213402 6.50552783994171E-4 80-81 7.434888959933384E-4 4.646805599958365E-5 0.0 2.7461227054073944 6.970208399937547E-4 82-83 7.434888959933384E-4 4.646805599958365E-5 0.0 2.971887755481372 7.89956951992922E-4 84-85 7.434888959933384E-4 9.29361119991673E-5 0.0 3.1919140006394002 7.89956951992922E-4 86-87 7.434888959933384E-4 1.3940416799875096E-4 0.0 3.4229531750693303 7.89956951992922E-4 88-89 7.89956951992922E-4 1.858722239983346E-4 0.0 3.674810038587074 7.89956951992922E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6560 0.0 48.036556 1 GTATCAA 10845 0.0 38.054607 1 TATCAAC 13420 0.0 30.437075 2 ATCAACG 13495 0.0 30.198149 3 TCAACGC 13710 0.0 29.759266 4 CAACGCA 13920 0.0 29.276152 5 AACGCAG 14315 0.0 28.49424 6 ACGCAGA 16025 0.0 25.539692 7 CGCAGAG 16120 0.0 25.209318 8 GAGTACT 9665 0.0 24.12307 12-13 GTACATG 9675 0.0 24.058887 1 TACATGG 9575 0.0 23.58941 2 GCAGAGT 17115 0.0 22.966173 9 GTACTTT 10240 0.0 22.558567 14-15 TATAACG 190 3.2556636E-7 22.524294 2 TAAGGTG 1110 0.0 21.848398 5 ACATGGG 9990 0.0 21.705597 3 GTAAGGT 1075 0.0 21.232698 4 AGTACTT 10140 0.0 21.071096 12-13 AGAGTAC 14785 0.0 20.991661 10-11 >>END_MODULE