FastQCFastQC Report
Thu 26 May 2016
SRR938144_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938144_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2152016
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116650.5420498732351432No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105080.48828633244362496No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64150.29809257923732907No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27220.12648604843086667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68200.045.1308171
GTATCAA110300.035.8286441
TATCAAC134250.029.1524642
ATCAACG134450.029.0697213
TCAACGC136050.028.7271824
CAACGCA136600.028.6115175
AACGCAG141100.027.86
ACGCAGA160800.024.3055557
CGCAGAG161750.024.0453648
GAGTACT97700.023.5035812-13
GTACTTT101950.022.33744414-15
GCAGAGT176150.021.8370579
GTACATG91750.021.3809281
TAAGGTG11550.021.3802665
TACCTGG18050.021.3142572
GTGTACG2701.0022632E-921.1105751
TACATGG90050.020.8868942
GTACCTG19950.020.713761
AGTACTT101150.020.3542612-13
CAGAGTA167300.020.21208410-11
Produced by FastQC (version 0.11.5)