FastQCFastQC Report
Thu 26 May 2016
SRR938143_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938143_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2049370
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162720.7940001073500637No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135180.659617345818471No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85950.41939718059696396No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33560.16375764259260162No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25050.12223268614257066No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23690.1155965003879241No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65200.056.5459181
GTATCAA116950.041.679261
TATCAAC144700.033.3385962
ATCAACG146250.032.9852643
TCAACGC147950.032.606254
CAACGCA149750.032.1508455
AACGCAG153200.031.45176
GTGGTAT26850.027.888471
ACGCAGA173000.027.7092767
CGCAGAG174050.027.5148168
TGGTATC27100.026.3070282
GTACATG111300.025.7542341
GAGTACT102950.025.5398512-13
GCAGAGT183600.025.4865959
TACATGG116050.023.9995022
GTACTTT110450.023.91486514-15
ACATGGG117950.022.84733
CAGAGTA178350.022.64064610-11
AGTACTT106750.021.76046212-13
GTATAGA4850.021.634671
Produced by FastQC (version 0.11.5)