FastQCFastQC Report
Thu 26 May 2016
SRR938143_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938143_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2049370
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157470.7683824785177884No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137080.6688884876815803No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89320.4358412585331102No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34580.16873478190858654No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25960.12667307514016504No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23550.11491336361906342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68200.052.8661461
GTATCAA115500.039.576211
TATCAAC147300.030.9353892
ATCAACG147900.030.7066573
TCAACGC149050.030.5639614
CAACGCA151850.029.9057065
AACGCAG155700.029.1618616
GTGGTAT31100.026.9181421
ACGCAGA177350.025.4680257
CGCAGAG177600.025.4054368
GTACATG112200.025.0545961
TGGTATC33450.024.4582372
GAGTACT104150.024.35162512-13
TACATGG115350.024.2466952
GCAGAGT188800.023.5461469
GTACTTT109200.023.16023414-15
ACATGGG117750.022.8587683
AGTACTT107200.021.4213912-13
CAGAGTA184350.021.33207910-11
AGAGTAC174950.020.38787510-11
Produced by FastQC (version 0.11.5)